Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,263,502 T→C 9.2% A53A (GCA→GCG tdcD ← propionate kinase/acetate kinase C, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,263,5020TC 9.2% 293.1 / 17.5 109A53A (GCA→GCGtdcDpropionate kinase/acetate kinase C, anaerobic
Reads supporting (aligned to +/- strand):  ref base T (51/46);  new base C (7/3);  total (60/49)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

Only 100 of 111 total aligned reads displayed.

TCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAA  >  NC_000913/3263353‑3263651
                                                                                                                                                     |                                                                                                                                                      
tCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCCCACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGt                                                                                                                                                        >  2:1274686/1‑150 (MQ=255)
 cATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTg                                                                                                                                                       >  1:479699/1‑150 (MQ=255)
  aTCGGTAATAATGGCGGACTCGGTAAAAAAACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCACTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGc                                                                                                                                                      <  2:272513/150‑1 (MQ=255)
     ggTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGTGGTGCTgg                                                                                                                                                   >  2:681389/1‑150 (MQ=255)
      gTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGc                                                                                                                                                  >  2:1304274/1‑150 (MQ=255)
        aataatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCAGCACCGCTGGctca                                                                                                                                               <  2:1633077/150‑2 (MQ=255)
        aataatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGAGAGCCAGCACCGCTGGctc                                                                                                                                                <  2:778485/150‑1 (MQ=255)
          taatGGCGGACTCGGTAAAAATTCTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGctcac                                                                                                                                              <  2:475881/150‑3 (MQ=255)
             tGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTGCAGTTCAAATGCAATTGCCTTCACTGCACCTTCGTAGCTGTGGTGAGCCCGCGGTGCTGGGTCTcccc                                                                                                                                          >  2:1133349/1‑150 (MQ=255)
             tGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcccc                                                                                                                                          <  2:1012610/150‑1 (MQ=255)
             tGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcccc                                                                                                                                          >  2:647184/1‑150 (MQ=255)
             tGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcccc                                                                                                                                          <  1:447070/150‑1 (MQ=255)
                  gACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCCGCGGTGCTGGCGCTCCCCCAgtt                                                                                                                                     >  2:618859/1‑150 (MQ=255)
                     tCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACg                                                                                                                                  <  1:1363735/150‑1 (MQ=255)
                      cGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCCGGCGTGCTGGGGCTCCCCCATTTACgg                                                                                                                                 >  1:1666343/1‑150 (MQ=255)
                      cGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACgg                                                                                                                                 >  2:87279/1‑150 (MQ=255)
                      cGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACgg                                                                                                                                 <  2:979832/150‑1 (MQ=255)
                       ggTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGa                                                                                                                                >  1:77548/1‑150 (MQ=255)
                       ggTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCATCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGa                                                                                                                                <  1:1064199/150‑1 (MQ=255)
                          aaaaaTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATaa                                                                                                                             >  1:1053892/1‑150 (MQ=255)
                          aaaaaTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAAATCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATaa                                                                                                                             <  2:1297201/150‑1 (MQ=255)
                            aaaTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCCGCGGTGGTGGGGCACCCCCCTATAAGGagaaga                                                                                                                           >  1:724457/1‑150 (MQ=255)
                             aaTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCCCCCCTGCTGGCGCTGCACCAATTAAGGAaaaaaa                                                                                                                          >  2:1380132/1‑150 (MQ=255)
                              aTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAAt                                                                                                                         <  2:1694210/150‑1 (MQ=255)
                               tACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATg                                                                                                                        <  1:542333/150‑1 (MQ=255)
                                 cTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGGCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCa                                                                                                                      <  1:156034/150‑1 (MQ=255)
                                   gccgccGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCACAGGTGATGGCTATCCCCCAATTACCGTTAATAATTCAtt                                                                                                                    >  2:176152/1‑150 (MQ=255)
                                      gccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGGGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTc                                                                                                                 <  2:55846/150‑1 (MQ=255)
                                          tGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAg                                                                                                             <  1:794076/150‑1 (MQ=255)
                                            aGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTGTTCCAGTTCACATGAAATTGCCTTCACTGCACCTTCGTAGCTGTGGTGCGCCAGCGGTGCTGGCTCGCCCCCATGTCCGGCTAAGAATGCATTTTCCGAGtt                                                                                                           >  1:80102/1‑150 (MQ=255)
                                             gCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTTGCTCTCCCCCATTTAAGGATAAGAAAGCATTTTCCGAGTTa                                                                                                          >  2:138533/1‑150 (MQ=255)
                                             gCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTa                                                                                                          <  2:933339/150‑1 (MQ=255)
                                             gCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTa                                                                                                          <  1:1627258/150‑1 (MQ=255)
                                             gCGATGCGGTGGCCAATTAAGGCCACACCGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTa                                                                                                          >  2:945640/1‑150 (MQ=255)
                                              cGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTaa                                                                                                         <  1:597393/150‑1 (MQ=255)
                                               gATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAAt                                                                                                        <  2:157010/150‑1 (MQ=255)
                                                  gCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCAGCGGTGCTGGCTCTCCCCCCTTTACGGATAAGAATGCATTTTGCGAGTTAATTcc                                                                                                     >  1:421817/1‑150 (MQ=255)
                                                   cGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAACTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCGAGCGGTTCTGGCTCTACCCCATTTACGGTTAAGAATGCATTTTGCGAGTTAATACCg                                                                                                    >  1:86099/1‑150 (MQ=255)
                                                      tGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCg                                                                                                 <  2:611258/150‑1 (MQ=255)
                                                       ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCAGCGGGTGTGGGGCTCCCCCAATTAAGGTTAAAAAAAAATTTTACGAGTTtataacctagg                                                                                                >  1:671875/1‑143 (MQ=255)
                                                       ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                                >  2:956955/1‑150 (MQ=255)
                                                        gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGc                                                                                               <  1:1506949/150‑1 (MQ=255)
                                                            aTTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTTGTGGCTCTACCCCAATTTAGGATAATAAATCATTTTACGAGTTAAAAACGTAGGGaaaa                                                                                           >  2:1420519/1‑148 (MQ=255)
                                                            aTTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGACAGCGGTGCTTGCTATACCCCATTTACGGATAAGAATGGATTTTACGAGTTAATAACCTACGCAATa                                                                                           >  1:315142/1‑150 (MQ=255)
                                                              tAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCACCGGTGCGTGCTCTACCCCCATTTAGGTTAATAATTCATTTTACCAGTTTATACCCTACGCAATaaa                                                                                         >  2:1628519/1‑148 (MQ=255)
                                                              acccgCCACACTCGCATTTAAATTCCCTTATTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCGCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATAcc                                                                                         <  2:1200293/146‑1 (MQ=255)
                                                                aGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTg                                                                                       <  1:1516781/150‑1 (MQ=255)
                                                                 ggCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTTGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                      >  2:1233151/1‑150 (MQ=255)
                                                                  gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTTAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGAc                                                                                     <  1:1380132/150‑1 (MQ=255)
                                                                  gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGAc                                                                                     <  1:136160/150‑1 (MQ=255)
                                                                  gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCACCGGTGGTGGGGCTCCCCCCGTTACGGATTAGAATGGATTTTGCGAGTTAATACCGTCGGCAAAACCAGaa                                                                                     >  2:469893/1‑149 (MQ=255)
                                                                        cTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCCGTGCTGGGTGTACCCCATTTAAGGATTAGAATGCAATTTACGAGTTAATTACGTCGGCAATACCAGACATTAAt                                                                               >  1:529375/1‑150 (MQ=255)
                                                                             aTTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCAGCGGTGCTGGCTCTCCCCCAATTACGCATAATAAATGATTTTACGAGTTAATAACGTCGGCAAAAACTGAAAataataaata                                                                          >  2:1818467/1‑146 (MQ=255)
                                                                             aTTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATACACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCATTTAAGGATAAGAATGCATTTTACGAGTTAATTCCGTCGGCAATTACTGAAAATAATACttaa                                                                         >  1:1194402/1‑148 (MQ=255)
                                                                             aTTTAAATTCCGATTTTCCATTTCAAATGCAATTGCCTTCAATGCAACTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTc                                                                          <  2:888550/150‑1 (MQ=255)
                                                                               ttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGATCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcac                                                                        <  1:324946/150‑1 (MQ=255)
                                                                                tAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCCGCGGGTCGGGCACACCCCCCTTTAAGGATTATAATTCAATTTAAACGTTTATTACGTCGGGAATAACTGTCATTAATaaatgaaa                                                                       >  2:1148260/1‑143 (MQ=255)
                                                                                   aTTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTc                                                                    >  2:1233370/1‑150 (MQ=255)
                                                                                      ccGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCCTTTAAGGATAAGAATTCATTTTACGAGTTAATACCGTCGGCAATACCTGACATTAAAAATTAAAAGTCGcg                                                                 >  2:1728866/1‑149 (MQ=255)
                                                                                       cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCAGCGGTGCTGGCTCGCCCCCCTTTACGGATAATAAAGCATTTTCCGAGTTTATACCGTCGGCAATACCGTACATTATTAAATAACAGTCGCTg                                                                >  1:1228974/1‑150 (MQ=255)
                                                                                       cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTg                                                                <  2:479699/150‑1 (MQ=255)
                                                                                       cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTg                                                                >  2:216468/1‑150 (MQ=255)
                                                                                       cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTg                                                                >  2:25630/1‑150 (MQ=255)
                                                                                       cGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAATTACGGATAAGAATGCATTTTACGAGTTAATACCGTCGGCAATACCGGACATTAATACTTAAAAGTCGCTg                                                                >  2:1002403/1‑150 (MQ=255)
                                                                                             tCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCg                                                          >  1:1023541/1‑150 (MQ=255)
                                                                                                aGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGAATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGc                                                       <  1:647184/150‑1 (MQ=255)
                                                                                                 gTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCa                                                      >  2:1065701/1‑150 (MQ=255)
                                                                                                  ttCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTATCCCCCATTTACGGATAAGAAGGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCAc                                                     >  2:1044149/1‑150 (MQ=255)
                                                                                                  ttCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGACCCAGCGGTGCTGGCTCACCCCAATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGGCGGCAATACCTGACATTTATACTTCACAGTCGCTTGCATAGAGCAc                                                     >  2:1030368/1‑150 (MQ=255)
                                                                                                    cAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCCGCGGTGGTGGCGGTACCCCAATTAAGGATAAAAAAGCATTTTACGACTTAATACCCGCGGGAATAACAGACATTAAAAATTAACACTAGCTGGCGTagaggcagg                                                   >  2:908642/1‑145 (MQ=255)
                                                                                                       aTGCAATTGCCTTCAATGCACCTTCGTAGCTTTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGaaa                                                <  2:31177/150‑1 (MQ=255)
                                                                                                         gCAATTTCCTTCAATGCACCTACTTAGCTGTCGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAAGGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAAc                                              <  1:1155478/150‑1 (MQ=255)
                                                                                                         gCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAAc                                              <  1:1739498/150‑1 (MQ=255)
                                                                                                          cAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACt                                             <  2:1023541/150‑1 (MQ=255)
                                                                                                           aaTTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACtt                                            <  2:394559/150‑1 (MQ=255)
                                                                                                             ttGCCTTCAATGCACCTTCGTAGCTGTGGGGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTaa                                          <  2:637497/150‑1 (MQ=255)
                                                                                                                ccTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCg                                       <  1:24162/150‑1 (MQ=255)
                                                                                                                   tCAATGCACCTTCGTAGCTGTGGTGCGCCAGCGGTGCTGGCGCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTATTACCGTCGGCACGCCCTGACGTTAATACTTCACAGTCGCGGGCCTCGAGCCCGGAAACCGAAATCGCAg                                    >  1:287860/1‑150 (MQ=255)
                                                                                                                      aTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACg                                 <  2:1603823/150‑1 (MQ=255)
                                                                                                                        gCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGaa                               >  1:1032632/1‑150 (MQ=255)
                                                                                                                          aCCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAcc                             <  1:176152/150‑1 (MQ=255)
                                                                                                                                   gCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACCGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATAACTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATAGAAGACGCACCACAGTtaat                    >  2:1033145/1‑150 (MQ=255)
                                                                                                                                    tgggggagAGCCAGCACCGCTGGCTCACCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaata                   >  1:1240814/8‑150 (MQ=255)
                                                                                                                                     tgtgGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaataa                  >  1:1077882/1‑150 (MQ=255)
                                                                                                                                      ggggagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAc                 >  2:1436669/6‑150 (MQ=255)
                                                                                                                                       tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAcc                <  1:1042653/150‑1 (MQ=255)
                                                                                                                                       gggagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAcc                >  1:731369/5‑150 (MQ=255)
                                                                                                                                        ggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCa               >  1:1628800/1‑150 (MQ=255)
                                                                                                                                        ggagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCCAAACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGCACCACAGTTAATAACCa               >  1:245098/4‑150 (MQ=255)
                                                                                                                                        ggagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCa               >  2:1817194/4‑150 (MQ=255)
                                                                                                                                        ggagAGCCAGCACCGCTGGCTCACCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCa               >  1:1449002/4‑150 (MQ=255)
                                                                                                                                             gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaaca          >  2:1537479/1‑150 (MQ=255)
                                                                                                                                             gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaaca          <  1:530442/150‑1 (MQ=255)
                                                                                                                                             gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaaca          >  1:1231758/1‑150 (MQ=255)
                                                                                                                                             gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTAACAGTCGCTGGCATCGAGCACGGATAACTTAATAGAAGACGAACCACATTTAATAAACAAaacc          >  2:930556/1‑149 (MQ=255)
                                                                                                                                              ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCCCGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaacac         >  1:1030636/1‑149 (MQ=255)
                                                                                                                                               cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaacaac        >  1:799601/1‑150 (MQ=255)
                                                                                                                                                 gCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCg      <  2:1043417/150‑1 (MQ=255)
                                                                                                                                                 gCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCg      <  2:174574/150‑1 (MQ=255)
                                                                                                                                                     tGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGaaa  >  1:1165658/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAA  >  NC_000913/3263353‑3263651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: