Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,282,928 T→A 5.3% I318N (ATC→AAC) ‡ agaS → tagatose‑6‑phosphate ketose/aldose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,282,9280TA 5.3% 331.2 / 6.2 113I318N (ATC→AAC) ‡agaStagatose‑6‑phosphate ketose/aldose isomerase
Reads supporting (aligned to +/- strand):  ref base T (65/42);  new base A (2/4);  total (67/46)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01

Only 100 of 116 total aligned reads displayed.

AATCGCTGGTCGATGACGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTG  >  NC_000913/3282781‑3283077
                                                                                                                                                   |                                                                                                                                                     
aaTCGCTGGTCGATGACGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACAtcg                                                                                                                                                     >  1:1313094/1‑150 (MQ=255)
 aTCGCTGGTCGATGACGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACAtcgt                                                                                                                                                    <  2:1042013/150‑1 (MQ=255)
    gCTGGTCGATGACGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGc                                                                                                                                                 >  2:938181/1‑150 (MQ=255)
       ggTCGATGACGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACGATGTCGCTGc                                                                                                                                              <  2:1171594/150‑1 (MQ=255)
        gTCGATGACGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACGATGTCGCTGct                                                                                                                                             <  1:14549/150‑2 (MQ=255)
         tCGATGACGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTTCTGGCTGACCTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCg                                                                                                                                            >  1:1044944/1‑150 (MQ=255)
          cGATGACGAAACGCTGGTGGTGGTCTTTGTCACCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCgg                                                                                                                                           <  1:1098724/150‑1 (MQ=255)
          cGATGACGAAACGCTGGTGGTGGTATTTGTCTCCATCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGATGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAACGCAGCGACATCGTCGCTGCCgg                                                                                                                                           >  2:954019/1‑150 (MQ=255)
          cGATGACGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCgg                                                                                                                                           <  2:1450249/150‑1 (MQ=255)
            aTGACGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTc                                                                                                                                         >  1:742/1‑150 (MQ=255)
                cGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCcaca                                                                                                                                     <  1:1800584/150‑1 (MQ=255)
                 gAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACat                                                                                                                                    <  1:493073/150‑1 (MQ=255)
                  aaaCGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACata                                                                                                                                   >  1:1301242/1‑150 (MQ=255)
                    aCGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCCGGCAATGCGTGTAATCGCCATCGCCGCGGAACGCAGCGACATCGTCGCTGCCGGTCCACATatc                                                                                                                                 <  2:1408394/150‑1 (MQ=255)
                        tggtggtggTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATcc                                                                                                                             >  2:172228/1‑150 (MQ=255)
                         ggtgggggtATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGCTCTGCTGGCTGACCTTCGCCGTGACAACCAGGCAATGCGTTTAATCGCCATCGCCGCGGAAAGCACCGACATCGTCGCTGCCGGTCCACATATCATCCt                                                                                                                            <  2:262228/150‑1 (MQ=255)
                          gtggtggtATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTg                                                                                                                           <  2:354478/150‑1 (MQ=255)
                              tggtATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACACCCAGGCAATGCGAGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGACGGTCCACATATCATCCTGCCAc                                                                                                                       >  2:1709981/1‑150 (MQ=255)
                                gtATTTGGCTCCAGCCACCCTTACACCCGTAAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCAGCGCCGCGGAAAGCAGCGACATCGGCGCTGCCGGTCCACATATCATCCTGCCACcg                                                                                                                     <  2:659672/150‑1 (MQ=255)
                                 tATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCCCcgt                                                                                                                    >  1:1110688/1‑150 (MQ=255)
                                  aTTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtc                                                                                                                   <  2:1232343/150‑1 (MQ=255)
                                    ttGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtcac                                                                                                                 >  2:223129/1‑150 (MQ=255)
                                     tGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtcacg                                                                                                                >  2:1441400/1‑150 (MQ=255)
                                      gTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCCGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtcacgt                                                                                                               >  1:1328347/1‑150 (MQ=255)
                                      gTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtcacgt                                                                                                               <  2:1085830/150‑1 (MQ=255)
                                      gTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtcacgt                                                                                                               >  2:118234/1‑150 (MQ=255)
                                       tctcCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtcacgtc                                                                                                              <  2:1071541/150‑1 (MQ=255)
                                        ctcCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCCGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtcacgtca                                                                                                             >  2:710835/1‑150 (MQ=255)
                                            aGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACttt                                                                                                         >  1:837647/1‑150 (MQ=255)
                                               cACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCGGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATc                                                                                                      <  2:1816582/150‑1 (MQ=255)
                                                aCCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCg                                                                                                     >  2:834860/1‑150 (MQ=255)
                                                    ttACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGt                                                                                                 <  2:1313094/150‑1 (MQ=255)
                                                     tACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGtt                                                                                                >  2:1744277/1‑150 (MQ=255)
                                                      acacCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTg                                                                                               <  2:471450/150‑1 (MQ=255)
                                                       cacCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGa                                                                                              >  1:901444/1‑150 (MQ=255)
                                                         cccGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGc                                                                                            >  1:1794823/1‑150 (MQ=255)
                                                                 tATGATCTTGATCTGCTGTCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCAttt                                                                                    <  2:689270/150‑1 (MQ=255)
                                                                 tATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCAttt                                                                                    <  1:434396/150‑1 (MQ=255)
                                                                    gatcttgatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCCCCGTCACGTCCCTTTATCGACGTTGAGCAGGCATTTTGc                                                                                 >  1:1344585/1‑150 (MQ=255)
                                                                      tcttgatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAACGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCtt                                                                               >  2:343000/1‑150 (MQ=255)
                                                                      tcttgatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCtt                                                                               <  1:613155/150‑1 (MQ=255)
                                                                       cttgatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGTGACATCGTCGCTGCCGGTCCAAATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTc                                                                              >  2:827063/1‑150 (MQ=255)
                                                                       cttgatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTc                                                                              >  2:376799/1‑150 (MQ=255)
                                                                       cttgatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTc                                                                              >  2:1470844/1‑150 (MQ=255)
                                                                        ttgatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTcc                                                                             <  1:759158/150‑1 (MQ=255)
                                                                         agatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGATGCCGGTCCACATATCATCCTGCCACCGTCAAGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCt                                                                            >  2:1732394/2‑150 (MQ=255)
                                                                          gatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCTTTTTGCTTCCTg                                                                           >  1:1519638/1‑150 (MQ=255)
                                                                          gatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTg                                                                           <  2:938679/150‑1 (MQ=255)
                                                                           atctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa                                                                          <  1:460218/150‑1 (MQ=255)
                                                                           atctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa                                                                          <  1:1540885/150‑1 (MQ=255)
                                                                             ctgctgGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATg                                                                        <  1:664176/150‑1 (MQ=255)
                                                                             ctgctgGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATg                                                                        >  2:149399/1‑150 (MQ=255)
                                                                             ctgctgGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATg                                                                        <  2:1150129/150‑1 (MQ=255)
                                                                                ctggctgAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTACACATATCATCCTGCCCCCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTAc                                                                     >  1:373855/1‑150 (MQ=255)
                                                                                   gctgAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGcc                                                                  <  1:554547/150‑1 (MQ=255)
                                                                                    ctgAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGccc                                                                 >  1:1583822/1‑150 (MQ=255)
                                                                                    ctgAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGccc                                                                 >  2:1694519/1‑150 (MQ=255)
                                                                                    ctgAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGccc                                                                 >  2:1782981/1‑150 (MQ=255)
                                                                                    ctgAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGccc                                                                 >  2:859842/1‑150 (MQ=255)
                                                                                        aCTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGAc                                                             <  1:709624/150‑1 (MQ=255)
                                                                                         cTTCGCCGTGACAACCCGGCAATGCGGGTAATCGCCATCGCCGCGGAACGCCGCGACATCGTCGCGGCCGGTCCACATATCATCCTGCCCCCGTCCCGTCACTGTATCGACGGTGAGCAGGCAGTTTGCTTCCTGATGTACGACCAGACg                                                            >  1:267341/1‑150 (MQ=255)
                                                                                          ttCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGt                                                           >  2:1034661/1‑150 (MQ=255)
                                                                                             gCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTGTATCGACGTTGAGCAGGGATTTTGCGTACTGATGTACCCCCAGCCGTTTg                                                        >  2:907056/1‑150 (MQ=255)
                                                                                              ccGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGc                                                       >  2:805535/1‑150 (MQ=255)
                                                                                                gTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCAc                                                     <  1:1232819/150‑1 (MQ=255)
                                                                                                gTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCAc                                                     >  1:1701043/1‑150 (MQ=255)
                                                                                                 tGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACt                                                    <  1:1425885/150‑1 (MQ=255)
                                                                                                 tGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACt                                                    >  1:247411/1‑150 (MQ=255)
                                                                                                  gACAACCTGGCAATGCGTGGAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTg                                                   <  2:1602324/150‑1 (MQ=255)
                                                                                                    cAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGAt                                                 >  1:1106781/1‑150 (MQ=255)
                                                                                                       ccAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCa                                              >  1:22442/1‑150 (MQ=255)
                                                                                                          ggCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTc                                           <  2:1747634/150‑1 (MQ=255)
                                                                                                           gCAATGCGTGTAAACGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCTGTCCACATATCTTCGTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCg                                          <  1:118234/150‑1 (MQ=255)
                                                                                                            cAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGc                                         <  1:375811/150‑1 (MQ=255)
                                                                                                             aaTGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCt                                        >  2:369115/1‑150 (MQ=255)
                                                                                                             aaTGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCt                                        <  1:402634/150‑1 (MQ=255)
                                                                                                               tGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGc                                      <  2:1162469/150‑1 (MQ=255)
                                                                                                               tGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGc                                      <  1:1738162/150‑1 (MQ=255)
                                                                                                               tGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGc                                      >  1:979353/1‑150 (MQ=255)
                                                                                                                 cGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGcac                                    >  1:1103034/1‑150 (MQ=255)
                                                                                                                   tgtAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACAt                                  >  1:1120131/1‑150 (MQ=255)
                                                                                                                      aaTCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATggg                               >  1:1559461/1‑150 (MQ=255)
                                                                                                                       atcgccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGc                              >  1:642828/1‑150 (MQ=255)
                                                                                                                        tcgccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGATGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTTCACTGATGCAGTCGCTGCACATGGGCa                             >  2:1207233/1‑150 (MQ=255)
                                                                                                                          gccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAAt                           >  1:1432099/1‑150 (MQ=255)
                                                                                                                               cgccgcGGAAAGCAGCGACATCGTCGCTGCCTGTCCACATATCATCCAGCCCCCGTCACCTCACATTATCGACGTTGAGCAGGCAATTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACAAGGGAAATACGcc                      >  1:182162/1‑150 (MQ=255)
                                                                                                                                gccgcGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCg                     <  2:500137/150‑1 (MQ=255)
                                                                                                                                 ccgcGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGATGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCgg                    >  2:1202865/1‑150 (MQ=255)
                                                                                                                                    cgGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATa                 <  2:901444/150‑1 (MQ=255)
                                                                                                                                     ggAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATAc                <  1:1694519/150‑1 (MQ=255)
                                                                                                                                     ggAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATAc                <  2:1745104/150‑1 (MQ=255)
                                                                                                                                     ggAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGATTGCACTGATGCAGACGCTGCACAAGGGCAATACGCCGGATAc                >  1:1110468/1‑150 (MQ=255)
                                                                                                                                      gAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATAcc               >  1:1228085/1‑150 (MQ=255)
                                                                                                                                        cggcagcGACGATGTCGCTGCTGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATAcccc             >  1:199506/3‑150 (MQ=255)
                                                                                                                                           cagcGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATc          >  2:15292/1‑150 (MQ=255)
                                                                                                                                           cagcGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATc          >  1:1458941/1‑150 (MQ=255)
                                                                                                                                            agcGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCCCTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGCTGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCa         >  2:1482515/1‑150 (MQ=255)
                                                                                                                                             gcGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAg        >  2:1033748/1‑150 (MQ=255)
                                                                                                                                               gACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGcc      >  1:661983/1‑150 (MQ=255)
                                                                                                                                                   tcgtcgCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTg  <  1:427042/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AATCGCTGGTCGATGACGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTG  >  NC_000913/3282781‑3283077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: