Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 399,810 A→C 5.3% intergenic (+5/+19) yaiZ → / ← ddlA DUF2754 family putative inner membrane protein/D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913399,8100AC 5.3% 382.5 / 10.4 133intergenic (+5/+19)yaiZ/ddlADUF2754 family putative inner membrane protein/D‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base A (69/57);  new base C (5/2);  total (74/59)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.46e-01

Only 100 of 139 total aligned reads displayed.

CTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTG  >  NC_000913/399662‑399958
                                                                                                                                                    |                                                                                                                                                    
cTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAatat                                                                                                                                                     >  2:208789/1‑150 (MQ=255)
cTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAatat                                                                                                                                                     >  2:373677/1‑150 (MQ=255)
 ttAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAAtatt                                                                                                                                                    >  2:1577824/1‑150 (MQ=255)
    aTGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATc                                                                                                                                                 >  1:1329180/1‑150 (MQ=255)
    aTGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATc                                                                                                                                                 >  1:1321817/1‑150 (MQ=255)
        cGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGc                                                                                                                                             >  1:1400956/1‑150 (MQ=255)
         gtggtgGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCg                                                                                                                                            >  2:1223530/1‑150 (MQ=255)
             tggGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTaa                                                                                                                                        >  1:678767/1‑150 (MQ=255)
              ggggTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTaaa                                                                                                                                       >  2:1447576/1‑150 (MQ=255)
              ggggTGACTGGGATTTGAATCAAACGGTTGTCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCGATAATATTCTTATTGGGCGttttc                                                                                                                                       <  2:878776/150‑4 (MQ=255)
               ggggTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCTGTCTGGAGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTaaaa                                                                                                                                      <  1:1198369/150‑1 (MQ=255)
               gggTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCGATAATATTCTTATTGGGCGttttca                                                                                                                                      <  2:1796787/150‑5 (MQ=255)
                ggTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAat                                                                                                                                     >  2:787962/1‑150 (MQ=255)
                 gTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAata                                                                                                                                    >  1:1325269/1‑150 (MQ=255)
                   tACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATAtt                                                                                                                                  <  1:56480/150‑1 (MQ=255)
                      tGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACa                                                                                                                               >  2:34797/1‑150 (MQ=255)
                       gggATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGAATAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGAGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACAt                                                                                                                              >  1:176586/1‑150 (MQ=255)
                        ggATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCTGGCGTTAAAATATTACAtt                                                                                                                             >  2:73493/1‑150 (MQ=255)
                         gATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACAttt                                                                                                                            >  2:774518/1‑149 (MQ=255)
                          aTTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATtgt                                                                                                                           <  2:615061/150‑1 (MQ=255)
                            ttGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTgg                                                                                                                         >  1:437573/1‑150 (MQ=255)
                            ttGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTgg                                                                                                                         <  2:9259/150‑1 (MQ=255)
                            atGAACCAAAAGCGTTGCAGACCTATGCCGTCTGTCTGGTGACGTGTGTTATTATTTTCTGGATGGCGGGGTTGATTATTTGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTgg                                                                                                                         <  2:1190896/149‑1 (MQ=255)
                             tGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCGGGGGACGTGGGGGAGTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGCAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGt                                                                                                                        >  1:1076410/1‑150 (MQ=255)
                              gAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGtt                                                                                                                       >  1:1699098/1‑150 (MQ=255)
                               aaGCAAAAGGTTTGCAGACCTATGCCGTCGGGCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGttt                                                                                                                      <  2:344099/150‑1 (MQ=255)
                                aGCTAAAGGTTGGCAGTCCTATGCGGTCGGTCTGGTGACGGGGGTGTTTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGGCGCGATGCAGAAACTGAAAACGCCCAATATGAATATTATCGGTCGTTAAAATATTACATTGTGGtttt                                                                                                                     <  2:1176215/150‑1 (MQ=255)
                                 gCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTc                                                                                                                    >  1:1607283/1‑150 (MQ=255)
                                 gCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTc                                                                                                                    >  2:372559/1‑150 (MQ=255)
                                  cAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCa                                                                                                                   >  2:943499/1‑150 (MQ=255)
                                   aaaaGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCaa                                                                                                                  >  2:1277439/1‑150 (MQ=255)
                                   aaaaGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCaa                                                                                                                  >  1:677872/1‑150 (MQ=255)
                                   aaaaCGTTGGCAGACCTTTGCCGTCGGTCGGGTGACGTGGGTGATTAGTTTGTGGCTGGCGGGGTTGAATATTCGTCGTCGCGATGATCAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATAATACAATGTGCTTTTCaa                                                                                                                  >  2:451720/1‑150 (MQ=255)
                                      aGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGc                                                                                                               <  1:1707269/150‑1 (MQ=255)
                                      aGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGc                                                                                                               >  1:602300/1‑150 (MQ=255)
                                        gTTGTCAGACCTATGCCGTCGGTCTGGTGTCGTGGTTGATTAGTTTCTTGCTGGCGGGGTTGATTATTCGTCGTCGCTATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGt                                                                                                             <  1:1183487/150‑1 (MQ=255)
                                        gTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGt                                                                                                             <  1:550511/150‑1 (MQ=255)
                                         gtGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGtt                                                                                                            <  2:1566624/149‑1 (MQ=255)
                                            gCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATc                                                                                                         >  1:1389629/1‑150 (MQ=255)
                                              aGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTACAATGCGTTATCCg                                                                                                       >  2:306743/1‑150 (MQ=255)
                                                aCCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGTCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCa                                                                                                     <  2:1811899/150‑1 (MQ=255)
                                                aCCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCa                                                                                                     <  1:1596928/150‑1 (MQ=255)
                                                   tATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACTCCCAATAAGAATATTATCGGTCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCg                                                                                                  >  2:287690/1‑150 (MQ=255)
                                                    aTGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGACCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCTCAGCGt                                                                                                 >  2:1791081/1‑150 (MQ=255)
                                                           cGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTc                                                                                          <  1:577736/150‑1 (MQ=255)
                                                           cGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTc                                                                                          <  1:325237/150‑1 (MQ=255)
                                                            ggTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTcc                                                                                         >  1:1238358/1‑150 (MQ=255)
                                                               cttgttaCGTGGGTGATTAGTTTCTGGCTGTCGGGGTTGATTATTCGTCGTATCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCTGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAgc                                                                                      <  2:594886/144‑1 (MQ=255)
                                                                  gTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTTATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGcc                                                                                   <  2:1325269/150‑1 (MQ=255)
                                                                  gTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGGGTTAAAATATTACATTGTGGTTTTCAATGCGTTATACGAAGCGTGACGCTCCAGCGcc                                                                                   >  1:1821671/1‑150 (MQ=255)
                                                                    gACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAGTGCGTTATCCGCAGCGTGACGCTCCAGCGCCAg                                                                                 <  2:1485706/150‑1 (MQ=255)
                                                                      cGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGtt                                                                               <  1:816755/150‑1 (MQ=255)
                                                                       gTGGGTGATTAGTTTCTGGCTGGCTGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtc                                                                              <  1:780121/150‑1 (MQ=255)
                                                                         gggTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAAAAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaa                                                                            <  1:1198186/150‑1 (MQ=255)
                                                                           gTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCAATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaatc                                                                          >  2:289474/1‑150 (MQ=255)
                                                                            tGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaatca                                                                         <  1:1223530/150‑1 (MQ=255)
                                                                               ttAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAAc                                                                      <  2:678767/150‑1 (MQ=255)
                                                                                tAGTTTCTGTCTGGCGGTGTTGATTATTCGTCTTCGCGATGAAGAAACTGAAAACGACCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACg                                                                     <  2:1082952/150‑1 (MQ=255)
                                                                                     tCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGa                                                                <  2:551563/150‑1 (MQ=255)
                                                                                          ctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGAGATCAGa                                                           >  1:1716255/1‑150 (MQ=255)
                                                                                              cGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCgg                                                       <  1:787962/150‑1 (MQ=255)
                                                                                                  gTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTa                                                   <  1:1033125/150‑1 (MQ=255)
                                                                                                  gTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTa                                                   >  1:249326/1‑150 (MQ=255)
                                                                                                    tGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAAc                                                 <  2:1245767/150‑1 (MQ=255)
                                                                                                     gATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGTCGTTTAAATATTTCATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCATATCGGTGTAAcc                                                >  1:287375/1‑150 (MQ=255)
                                                                                                       taattCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCa                                              >  2:671489/3‑150 (MQ=255)
                                                                                                          ttCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCATCGTGACTCTCCATCGCCAGTTCAATCAAACGTGTGATCATATCGGTGTAACCCAGAc                                           >  2:1174737/1‑150 (MQ=255)
                                                                                                               cgtcgCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTg                                      <  1:803011/150‑1 (MQ=255)
                                                                                                                gtcgCGATGAAAAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCATCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTgg                                     >  1:1459395/1‑150 (MQ=255)
                                                                                                                 tcgCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGc                                    <  2:1026938/150‑1 (MQ=255)
                                                                                                                  cgcgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCt                                   <  2:78087/150‑1 (MQ=255)
                                                                                                                    cgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTg                                 >  2:1723129/1‑150 (MQ=255)
                                                                                                                    cgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTg                                 <  2:186234/150‑1 (MQ=255)
                                                                                                                    cgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTg                                 <  1:1563080/150‑1 (MQ=255)
                                                                                                                      aTGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGcc                               <  1:34797/150‑1 (MQ=255)
                                                                                                                      aTGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGcc                               <  1:966346/150‑1 (MQ=255)
                                                                                                                       tGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGccc                              >  1:1470637/1‑149 (MQ=255)
                                                                                                                        gaagaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCcac                             >  2:584196/1‑150 (MQ=255)
                                                                                                                          agaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCTCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAg                           >  2:883173/1‑150 (MQ=255)
                                                                                                                          agaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACACAGACCGCTGTCTTCCCACAg                           >  1:88660/1‑150 (MQ=255)
                                                                                                                           gaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGc                          <  1:289474/150‑1 (MQ=255)
                                                                                                                               cTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCg                      <  2:1607283/150‑1 (MQ=255)
                                                                                                                                ttttaaCGCCCGATAATAATCTTATTGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTTCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCgg                     >  2:751197/5‑150 (MQ=255)
                                                                                                                                 tttaaCGCCCGATAATATTCTTATTGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGa                    >  1:1599450/4‑150 (MQ=255)
                                                                                                                                 gAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACATCTTCGGa                    >  2:1119233/1‑150 (MQ=255)
                                                                                                                                 gAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGa                    <  2:19264/150‑1 (MQ=255)
                                                                                                                                  ttaaCGCCCGATAATATTCTTATTGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCTATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGat                   >  2:1314584/3‑150 (MQ=255)
                                                                                                                                  aaaaCGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGat                   <  2:755897/150‑1 (MQ=255)
                                                                                                                                  aaaaCGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGat                   <  1:373677/150‑1 (MQ=255)
                                                                                                                                  aaaaCGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTAATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGat                   <  1:208789/150‑1 (MQ=255)
                                                                                                                                  aaaaCGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGGGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGnt                   <  2:1329180/150‑1 (MQ=255)
                                                                                                                                   aaaCGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCCGACCGCTGGCTTGCCACAGCTTCGGata                  >  1:208150/1‑150 (MQ=255)
                                                                                                                                    aaCGCCCGATAATATTCTTATTGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatac                 >  2:364332/1‑150 (MQ=255)
                                                                                                                                    aaCGCCCGATAATATTCTTATTGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatac                 >  1:1496895/1‑150 (MQ=255)
                                                                                                                                       gCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatacata              >  2:1139593/1‑150 (MQ=255)
                                                                                                                                        cccAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatacatac             >  1:1088543/1‑150 (MQ=255)
                                                                                                                                          cAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTg           <  2:1389629/150‑1 (MQ=255)
                                                                                                                                               aGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTACAGCGCCAGTTCAATCAAACGTGTGAGCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATAtt      <  2:1736397/150‑1 (MQ=255)
                                                                                                                                                  atatTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAAAGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGt   <  1:306743/150‑1 (MQ=255)
                                                                                                                                                   tattatCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTg  >  1:1455707/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTTAATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTG  >  NC_000913/399662‑399958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: