Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,370,704 A→C 7.6% R111R (CGT→CGG nanE ← putative N‑acetylmannosamine‑6‑P epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,370,7040AC 7.6% 324.3 / 15.8 129R111R (CGT→CGGnanEputative N‑acetylmannosamine‑6‑P epimerase
Reads supporting (aligned to +/- strand):  ref base A (63/56);  new base C (6/4);  total (69/60)
Fisher's exact test for biased strand distribution p-value = 7.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

Only 100 of 131 total aligned reads displayed.

CAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCACG  >  NC_000913/3370556‑3370853
                                                                                                                                                    |                                                                                                                                                     
cAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGAGGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGAc                                                                                                                                                      <  1:1600035/150‑1 (MQ=255)
 aGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGAcg                                                                                                                                                     >  2:1541425/1‑150 (MQ=255)
      tGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCTGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACgcgggc                                                                                                                                                >  1:951339/1‑150 (MQ=255)
       ggTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACTGGACgcgggcg                                                                                                                                               >  2:1166678/1‑150 (MQ=255)
       ggTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACgcgggcg                                                                                                                                               <  1:35491/150‑1 (MQ=255)
       ggTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACgcgggcg                                                                                                                                               <  1:1527641/150‑1 (MQ=255)
        gTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACgcgggcgg                                                                                                                                              >  1:575487/1‑150 (MQ=255)
         tataGCCAGAAAGCGTAGTGCCAATAATTTCGGCTACCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGGAAACCGTGAGGGTGAATACGTGCCAGCAGCGTTTCAACCGGCACCGGCCGCGGGcggt                                                                                                                                             >  1:925623/1‑150 (MQ=255)
           taGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcg                                                                                                                                           >  2:1194685/1‑150 (MQ=255)
           taGCCAGAAAGCGTAGTGCCAAAAAATCCGGCGCCCAGCTTTTGGCATGCCAGGCCGTCTACCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGGGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcg                                                                                                                                           <  1:1125153/150‑1 (MQ=255)
             gCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcggt                                                                                                                                         >  2:1195306/1‑150 (MQ=255)
              ccAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCATTCGGTCATCGCCAGTAAACCGTTATGGTGAATACGTGCCATCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTg                                                                                                                                        <  2:1497657/150‑1 (MQ=255)
               cAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGc                                                                                                                                       <  2:645859/150‑1 (MQ=255)
               cAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGc                                                                                                                                       >  2:1176620/1‑150 (MQ=255)
                aGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGACCGCCCGCGTCCGGTGcc                                                                                                                                      <  2:1239175/150‑1 (MQ=255)
                  aaaGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGt                                                                                                                                    <  1:1756877/150‑1 (MQ=255)
                   aaGCGTAGTGCCAATACTTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTc                                                                                                                                   <  2:1162546/150‑1 (MQ=255)
                   aaGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTc                                                                                                                                   <  2:1531623/150‑1 (MQ=255)
                   aaGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGGCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTc                                                                                                                                   <  1:612526/150‑1 (MQ=255)
                    aGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGACCGCCCGCGTCCGGTGcctgtt                                                                                                                                  <  1:1351554/150‑5 (MQ=255)
                      cGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAAt                                                                                                                                <  2:1014050/150‑1 (MQ=255)
                          gTGCCAATATTTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTTAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCg                                                                                                                            <  2:1753438/150‑1 (MQ=255)
                           tGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGa                                                                                                                           >  2:1465493/1‑150 (MQ=255)
                             ccAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATa                                                                                                                         >  2:195697/1‑150 (MQ=255)
                              cAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATaa                                                                                                                        >  2:1336721/1‑150 (MQ=255)
                              cAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATaa                                                                                                                        <  1:1488741/150‑1 (MQ=255)
                               aataatTTCGGCTCCCAGCTTTTGGCACGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAAt                                                                                                                       >  1:457991/1‑150 (MQ=255)
                                       cGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCgcgc                                                                                                               >  2:1704553/1‑150 (MQ=255)
                                             ccAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCt                                                                                                         >  1:1189974/1‑150 (MQ=255)
                                              cAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTg                                                                                                        >  2:428879/1‑150 (MQ=255)
                                                gCTTTTGGCATGCCAGGACGTCTTCCTGCTTTTAGCAGTCGGTCATCGCCCGTAAACAGTGATGCTTAATACGTGCCAGCAGCGTTTCTACAGTCACAGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTgcg                                                                                                      <  2:211109/150‑1 (MQ=255)
                                                      ggCATGCCATGCCGTCTTCCGGCGGTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgcg                                                                                                <  1:364247/150‑1 (MQ=255)
                                                      ggCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgcg                                                                                                >  2:207298/1‑150 (MQ=255)
                                                      ggCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgcg                                                                                                <  2:1299840/150‑1 (MQ=255)
                                                       gCATGCCAGCCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAAAGGCGATAATGTCCGCGCCCGCCTGCGCCAgctc                                                                                               >  2:494473/1‑148 (MQ=255)
                                                        cATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCa                                                                                              >  1:978791/1‑150 (MQ=255)
                                                          tGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATc                                                                                            <  1:1195306/150‑1 (MQ=255)
                                                           gCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCa                                                                                           >  1:1295211/1‑150 (MQ=255)
                                                           gCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGGTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCa                                                                                           <  2:361031/150‑1 (MQ=255)
                                                            ccAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCaa                                                                                          <  1:520169/150‑1 (MQ=255)
                                                            ccAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACAGTGATGGTGAATACGTGCCAGCAGCGTTTCCACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCaa                                                                                          >  1:1039490/1‑150 (MQ=255)
                                                                gCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATc                                                                                      <  2:307547/150‑1 (MQ=255)
                                                                 ccGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCt                                                                                     >  2:1750962/1‑150 (MQ=255)
                                                                  cGTCTTCCCGAGTTGAGCAGTCGGTCAACGACAGTAAACATTGATGGATAAAACGTGCCAGCAGCGTTTCAACAGGCTCCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCtt                                                                                    <  2:1174943/150‑1 (MQ=255)
                                                                     cTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCaa                                                                                 <  1:1046185/150‑1 (MQ=255)
                                                                     cTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCaa                                                                                 <  2:1786609/150‑1 (MQ=255)
                                                                       tCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAatc                                                                               <  1:1058719/150‑2 (MQ=255)
                                                                       tCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAata                                                                               <  1:1058721/150‑1 (MQ=255)
                                                                        ccGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAatat                                                                              >  2:1113031/1‑150 (MQ=255)
                                                                        ccGGCGTTGAGCAGTCGGTCATCGCCAGGAAACCGTGAGGGTGAATACGTGCCATCAGCGTTTCAACAGGCACCGGACGCGGGCGTTCGTTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAatat                                                                              <  1:338915/150‑1 (MQ=255)
                                                                          ggCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAg                                                                            >  1:42985/1‑150 (MQ=255)
                                                                                gAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTg                                                                      >  1:986405/1‑150 (MQ=255)
                                                                                 aGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGa                                                                     >  2:1287365/1‑150 (MQ=255)
                                                                                  gCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGAt                                                                    <  1:220158/150‑1 (MQ=255)
                                                                                   cAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACCGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGGGATg                                                                   >  2:753981/1‑150 (MQ=255)
                                                                                   cAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATg                                                                   <  2:104494/150‑1 (MQ=255)
                                                                                   cAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATg                                                                   <  2:1667378/150‑1 (MQ=255)
                                                                                     gTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCg                                                                 <  2:517536/150‑1 (MQ=255)
                                                                                             tCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGGCGTGATGCGTACCGgag                                                         <  1:214613/150‑1 (MQ=255)
                                                                                             tCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGgag                                                         >  2:434225/1‑150 (MQ=255)
                                                                                              cGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGgaga                                                        >  2:1022262/1‑150 (MQ=255)
                                                                                               gCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGaa                                                       <  1:774254/150‑1 (MQ=255)
                                                                                               gCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGaa                                                       >  1:582024/1‑150 (MQ=255)
                                                                                                  aGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAAtcc                                                    <  1:1499405/150‑1 (MQ=255)
                                                                                                    tAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAAtcctc                                                  <  2:1524834/150‑1 (MQ=255)
                                                                                                       aCCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAg                                               >  1:40523/1‑150 (MQ=255)
                                                                                                        ccGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGa                                              >  2:411242/1‑150 (MQ=255)
                                                                                                        ccGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGa                                              >  1:1505214/1‑150 (MQ=255)
                                                                                                         cGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGAt                                             >  2:1477082/1‑150 (MQ=255)
                                                                                                            gATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGCCGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCCGCGCAGCAACATCTTCAAGATAGGCCGTCATGCGTACCGGAGAATCCTCCAGATcgc                                          >  1:243826/1‑150 (MQ=255)
                                                                                                                 tGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTc                                     <  1:1541425/150‑1 (MQ=255)
                                                                                                                    aTACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTcaca                                  <  1:428879/150‑1 (MQ=255)
                                                                                                                      aCGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAAt                                >  2:748339/1‑150 (MQ=255)
                                                                                                                        gTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTc                              <  1:1128065/150‑1 (MQ=255)
                                                                                                                         tGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTcc                             <  2:575487/150‑1 (MQ=255)
                                                                                                                          gCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCAAGCGCATCAAAAACTTCAATATAGGCCGAGATGCGAACCGGAGACTCCTCCAGATAGCAATTAACAATTCCa                            >  2:946027/1‑150 (MQ=255)
                                                                                                                           ccAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCATCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCaa                           <  1:855109/150‑1 (MQ=255)
                                                                                                                             agcagcGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCCGCGCATCAACATCTGCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGCTCGCGTTTCACAATTCCaata                         >  2:537883/1‑150 (MQ=255)
                                                                                                                                 gcGTTTCAACAGGCACCGGACGCTGGCGGTCTGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATTTAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCg                     <  2:925623/150‑1 (MQ=255)
                                                                                                                                 gcGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCg                     >  2:1806807/1‑150 (MQ=255)
                                                                                                                                 gcGTTGCAACAGGCACCGGACGCGGGCGGTCGTTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATAAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCg                     <  2:1060096/150‑1 (MQ=255)
                                                                                                                                  cGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCgg                    <  1:1176620/150‑1 (MQ=255)
                                                                                                                                   gTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGc                   >  1:771596/1‑150 (MQ=255)
                                                                                                                                   gTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGGGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGc                   <  1:1098073/150‑1 (MQ=255)
                                                                                                                                    tttCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCa                  <  1:1721151/150‑1 (MQ=255)
                                                                                                                                     ttCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCAc                 >  2:70257/1‑150 (MQ=255)
                                                                                                                                      attgacggCACCGACCGCCCGCGTCCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAAGATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCGAATAATCGGCCCg                >  2:1134139/7‑150 (MQ=255)
                                                                                                                                        aaCAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCCTCACCATCTTCAATATAGGCCGTGATGCGGACCGGAGAATCCGCCAGCTCGCGTTTCACAATTCCAATAATCGGCACGcg              >  1:313214/1‑149 (MQ=255)
                                                                                                                                        aaCAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGAAAAAGACCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGACCCGGACAATCCTCCAGATCGCGGTTCACAATTACAATACTCGGCACGcg              >  2:337589/1‑149 (MQ=255)
                                                                                                                                         gacggCACCGACCGCCCGCGTCCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTc             >  2:813324/4‑150 (MQ=255)
                                                                                                                                         aCAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTc             >  1:758390/1‑150 (MQ=255)
                                                                                                                                           cggCACCGACCGCCCGCGTCCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTcac           >  1:1480666/2‑150 (MQ=255)
                                                                                                                                            ggCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCGGCGCATCAACATGGTCAAGATAGGCCGGGATGCGTACCGGAGAAGACTCCAGATCGCGTTTCACATTTCCAATAATCGGCACGCTcacc          >  1:960278/1‑150 (MQ=255)
                                                                                                                                            ggCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTcacc          >  2:481267/1‑150 (MQ=255)
                                                                                                                                            ggCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGCCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAGTCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTcccc          >  1:1295724/1‑150 (MQ=255)
                                                                                                                                              cACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCTGCGCATCAACATCTTCAATTTAGGCCGTGATGCGTTCCGGAGAATCCTCCAGATCGCGTTACACAATTCCAATAATCGGCACGCTcacccc        >  2:960359/1‑148 (MQ=255)
                                                                                                                                              cACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTcaccac        >  1:1745165/1‑150 (MQ=255)
                                                                                                                                                 cGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGc     >  1:940026/1‑150 (MQ=255)
                                                                                                                                                  ggACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCa    <  1:458935/150‑1 (MQ=255)
                                                                                                                                                    aCGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCAcg  <  2:178346/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGGCGTGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCACG  >  NC_000913/3370556‑3370853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: