Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,370,708 G→C 6.5% P110R (CCG→CGG) ‡ nanE ← putative N‑acetylmannosamine‑6‑P epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,370,7080GC 6.5% 383.4 / 15.7 123P110R (CCG→CGG) ‡nanEputative N‑acetylmannosamine‑6‑P epimerase
Reads supporting (aligned to +/- strand):  ref base G (60/54);  new base C (4/4);  total (64/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.59e-01

Only 100 of 129 total aligned reads displayed.

TGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCACGCGTGG  >  NC_000913/3370562‑3370858
                                                                                                                                                  |                                                                                                                                                      
 ggTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACTGGACgcgggcg                                                                                                                                                    >  2:1166678/1‑150 (MQ=255)
  gTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACgcgggcgg                                                                                                                                                   >  1:575487/1‑150 (MQ=255)
   tataGCCAGAAAGCGTAGTGCCAATAATTTCGGCTACCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGGAAACCGTGAGGGTGAATACGTGCCAGCAGCGTTTCAACCGGCACCGGCCGCGGGcggt                                                                                                                                                  >  1:925623/1‑150 (MQ=255)
     taGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcg                                                                                                                                                >  2:1194685/1‑150 (MQ=255)
     taGCCAGAAAGCGTAGTGCCAAAAAATCCGGCGCCCAGCTTTTGGCATGCCAGGCCGTCTACCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGGGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcg                                                                                                                                                <  1:1125153/150‑1 (MQ=255)
      aGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcgg                                                                                                                                               >  2:855109/1‑150 (MQ=255)
       gCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcggt                                                                                                                                              >  2:1195306/1‑150 (MQ=255)
        ccAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCATTCGGTCATCGCCAGTAAACCGTTATGGTGAATACGTGCCATCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTg                                                                                                                                             <  2:1497657/150‑1 (MQ=255)
         cAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGc                                                                                                                                            >  2:1176620/1‑150 (MQ=255)
         cAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGc                                                                                                                                            <  2:645859/150‑1 (MQ=255)
          aGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGACCGCCCGCGTCCGGTGcc                                                                                                                                           <  2:1239175/150‑1 (MQ=255)
            aaaGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGt                                                                                                                                         <  1:1756877/150‑1 (MQ=255)
              aGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGACCGCCCGCGTCCGGTGcctgtt                                                                                                                                       <  1:1351554/150‑5 (MQ=255)
                cGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAAt                                                                                                                                     <  2:1014050/150‑1 (MQ=255)
                       ccAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATa                                                                                                                              >  2:195697/1‑150 (MQ=255)
                        cAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATaa                                                                                                                             <  1:1488741/150‑1 (MQ=255)
                        cAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATaa                                                                                                                             >  2:1336721/1‑150 (MQ=255)
                         aataatTTCGGCTCCCAGCTTTTGGCACGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAAt                                                                                                                            >  1:457991/1‑150 (MQ=255)
                                 cGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCgcgc                                                                                                                    >  2:1704553/1‑150 (MQ=255)
                                      cccAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCTCCGGACGCGTGCGGTCGGTGCCGTCAATGGCGATAATGTCCGcgcccgcc                                                                                                               <  1:1365170/150‑1 (MQ=255)
                                       ccAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCt                                                                                                              >  1:1189974/1‑150 (MQ=255)
                                       ccAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGCCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCt                                                                                                              >  2:1458029/1‑150 (MQ=255)
                                        cAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTg                                                                                                             >  2:428879/1‑150 (MQ=255)
                                          gCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTgcg                                                                                                           >  1:1293442/1‑150 (MQ=255)
                                          gCTTTTGGCATGCCAGGACGTCTTCCTGCTTTTAGCAGTCGGTCATCGCCCGTAAACAGTGATGCTTAATACGTGCCAGCAGCGTTTCTACAGTCACAGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTgcg                                                                                                           <  2:211109/150‑1 (MQ=255)
                                                ggCATGCCATGCCGTCTTCCGGCGGTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgcg                                                                                                     <  1:364247/150‑1 (MQ=255)
                                                ggCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgcg                                                                                                     <  2:1299840/150‑1 (MQ=255)
                                                ggCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgcg                                                                                                     >  2:207298/1‑150 (MQ=255)
                                                ggCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgcg                                                                                                     >  2:1139736/1‑150 (MQ=255)
                                                 gCATGCCAGCCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAAAGGCGATAATGTCCGCGCCCGCCTGCGCCAgctc                                                                                                    >  2:494473/1‑148 (MQ=255)
                                                  cATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCa                                                                                                   >  1:978791/1‑150 (MQ=255)
                                                    tGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATc                                                                                                 <  1:1195306/150‑1 (MQ=255)
                                                    tGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGGGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATc                                                                                                 <  1:951456/150‑1 (MQ=255)
                                                     gCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGGTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCa                                                                                                <  2:361031/150‑1 (MQ=255)
                                                      ccAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACAGTGATGGTGAATACGTGCCAGCAGCGTTTCCACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCaa                                                                                               >  1:1039490/1‑150 (MQ=255)
                                                       cAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAAc                                                                                              >  1:276666/1‑150 (MQ=255)
                                                          gCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATc                                                                                           <  2:307547/150‑1 (MQ=255)
                                                           ccGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCt                                                                                          >  2:1750962/1‑150 (MQ=255)
                                                            cGTCTTCCCGAGTTGAGCAGTCGGTCAACGACAGTAAACATTGATGGATAAAACGTGCCAGCAGCGTTTCAACAGGCTCCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCtt                                                                                         <  2:1174943/150‑1 (MQ=255)
                                                               cTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCaa                                                                                      <  1:1046185/150‑1 (MQ=255)
                                                               cTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCaa                                                                                      <  2:1786609/150‑1 (MQ=255)
                                                                ttCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAat                                                                                     <  2:711499/150‑1 (MQ=255)
                                                                 tCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAatc                                                                                    <  1:1058719/150‑2 (MQ=255)
                                                                 tCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAata                                                                                    <  1:1058721/150‑1 (MQ=255)
                                                                  ccGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAatat                                                                                   <  2:1806823/150‑1 (MQ=255)
                                                                  ccGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAatat                                                                                   >  2:1113031/1‑150 (MQ=255)
                                                                  ccGGCGTTGAGCAGTCGGTCATCGCCAGGAAACCGTGAGGGTGAATACGTGCCATCAGCGTTTCAACAGGCACCGGACGCGGGCGTTCGTTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAatat                                                                                   <  1:338915/150‑1 (MQ=255)
                                                                   cGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGCTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAAtata                                                                                  >  1:391467/1‑150 (MQ=255)
                                                                          gAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTg                                                                           >  1:986405/1‑150 (MQ=255)
                                                                            gCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGAt                                                                         <  1:220158/150‑1 (MQ=255)
                                                                             cAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACCGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGGGATg                                                                        >  2:753981/1‑150 (MQ=255)
                                                                             cAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATg                                                                        <  2:1667378/150‑1 (MQ=255)
                                                                             cAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATg                                                                        <  2:104494/150‑1 (MQ=255)
                                                                              aGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGGCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGc                                                                       <  1:707497/150‑1 (MQ=255)
                                                                               gTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCg                                                                      <  2:517536/150‑1 (MQ=255)
                                                                                 cGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTa                                                                    >  2:1611535/1‑150 (MQ=255)
                                                                                  ggTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTAc                                                                   <  1:455416/150‑1 (MQ=255)
                                                                                     cATCGCCAGTAAACCGTGATGGTTAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACAgg                                                                <  1:1512709/150‑1 (MQ=255)
                                                                                       tCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGGCGTGATGCGTACCGgag                                                              <  1:214613/150‑1 (MQ=255)
                                                                                       tCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGgag                                                              >  2:434225/1‑150 (MQ=255)
                                                                                        cGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGgaga                                                             >  2:1022262/1‑150 (MQ=255)
                                                                                         gCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGaa                                                            <  1:774254/150‑1 (MQ=255)
                                                                                         gCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGaa                                                            >  1:582024/1‑150 (MQ=255)
                                                                                            aGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAAtcc                                                         <  1:1499405/150‑1 (MQ=255)
                                                                                              tAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAAtcctc                                                       <  2:1524834/150‑1 (MQ=255)
                                                                                                 aCCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAg                                                    >  1:40523/1‑150 (MQ=255)
                                                                                                  ccGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGa                                                   >  1:1505214/1‑150 (MQ=255)
                                                                                                  ccGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGa                                                   >  2:411242/1‑150 (MQ=255)
                                                                                                   cGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGAt                                                  >  2:1477082/1‑150 (MQ=255)
                                                                                                           tGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTc                                          <  1:1541425/150‑1 (MQ=255)
                                                                                                           tGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGGCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTc                                          <  2:1770942/150‑1 (MQ=255)
                                                                                                              aTACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTcaca                                       <  1:428879/150‑1 (MQ=255)
                                                                                                                  gTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTc                                   <  1:1128065/150‑1 (MQ=255)
                                                                                                                   tGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTcc                                  <  2:575487/150‑1 (MQ=255)
                                                                                                                   tGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTcc                                  <  1:1704553/150‑1 (MQ=255)
                                                                                                                    gCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCAAGCGCATCAAAAACTTCAATATAGGCCGAGATGCGAACCGGAGACTCCTCCAGATAGCAATTAACAATTCCa                                 >  2:946027/1‑150 (MQ=255)
                                                                                                                     ccAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCATCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCaa                                <  1:855109/150‑1 (MQ=255)
                                                                                                                       agcagcGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCCGCGCATCAACATCTGCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGCTCGCGTTTCACAATTCCaata                              >  2:537883/1‑150 (MQ=255)
                                                                                                                           gcGTTGCAACAGGCACCGGACGCGGGCGGTCGTTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATAAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCg                          <  2:1060096/150‑1 (MQ=255)
                                                                                                                            cGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCgg                         <  1:1176620/150‑1 (MQ=255)
                                                                                                                             gTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGc                        >  1:771596/1‑150 (MQ=255)
                                                                                                                             gTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGGGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGc                        <  1:1098073/150‑1 (MQ=255)
                                                                                                                              tttCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCa                       <  1:1721151/150‑1 (MQ=255)
                                                                                                                               ttCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCAc                      >  2:70257/1‑150 (MQ=255)
                                                                                                                                attgacggCACCGACCGCCCGCGTCCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAAGATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCGAATAATCGGCCCg                     >  2:1134139/7‑150 (MQ=255)
                                                                                                                                  aaCAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCCTCACCATCTTCAATATAGGCCGTGATGCGGACCGGAGAATCCGCCAGCTCGCGTTTCACAATTCCAATAATCGGCACGcg                   >  1:313214/1‑149 (MQ=255)
                                                                                                                                  aaCAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGAAAAAGACCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGACCCGGACAATCCTCCAGATCGCGGTTCACAATTACAATACTCGGCACGcg                   >  2:337589/1‑149 (MQ=255)
                                                                                                                                   gacggCACCGACCGCCCGCGTCCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTc                  >  2:813324/4‑150 (MQ=255)
                                                                                                                                   gacggCACCGACCGCCCGCGTCCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTc                  >  1:568554/4‑150 (MQ=255)
                                                                                                                                   aCAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTc                  >  1:758390/1‑150 (MQ=255)
                                                                                                                                      ggCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCGGCGCATCAACATGGTCAAGATAGGCCGGGATGCGTACCGGAGAAGACTCCAGATCGCGTTTCACATTTCCAATAATCGGCACGCTcacc               >  1:960278/1‑150 (MQ=255)
                                                                                                                                      ggCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTcacc               >  2:1240322/1‑150 (MQ=255)
                                                                                                                                      ggCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTcacc               >  2:481267/1‑150 (MQ=255)
                                                                                                                                      ggCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGCCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAGTCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTcccc               >  1:1295724/1‑150 (MQ=255)
                                                                                                                                        cACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCTGCGCATCAACATCTTCAATTTAGGCCGTGATGCGTTCCGGAGAATCCTCCAGATCGCGTTACACAATTCCAATAATCGGCACGCTcacccc             >  2:960359/1‑148 (MQ=255)
                                                                                                                                           cGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGc          >  1:940026/1‑150 (MQ=255)
                                                                                                                                            ggACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCa         <  1:458935/150‑1 (MQ=255)
                                                                                                                                              aCGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCAcg       <  1:195697/150‑1 (MQ=255)
                                                                                                                                              aCGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCAcg       <  2:178346/150‑1 (MQ=255)
                                                                                                                                                  gggcggTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACACCGCACGCGTgg  <  2:927197/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
TGGTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCACGCGTGG  >  NC_000913/3370562‑3370858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: