Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,370,713 G→T 7.2% D108E (GAC→GAA) ‡ nanE ← putative N‑acetylmannosamine‑6‑P epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,370,7130GT 7.2% 356.7 / 12.6 118D108E (GAC→GAA) ‡nanEputative N‑acetylmannosamine‑6‑P epimerase
Reads supporting (aligned to +/- strand):  ref base G (56/53);  new base T (4/5);  total (60/58)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 131 total aligned reads displayed.

GTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCACGCGTGGCTTG  >  NC_000913/3370564‑3370862
                                                                                                                                                     |                                                                                                                                                     
gTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACgcgggcgg                                                                                                                                                       >  1:575487/1‑150 (MQ=255)
 tataGCCAGAAAGCGTAGTGCCAATAATTTCGGCTACCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGGAAACCGTGAGGGTGAATACGTGCCAGCAGCGTTTCAACCGGCACCGGCCGCGGGcggt                                                                                                                                                      >  1:925623/1‑150 (MQ=255)
   taGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcg                                                                                                                                                    >  2:1194685/1‑150 (MQ=255)
   taGCCAGAAAGCGTAGTGCCAAAAAATCCGGCGCCCAGCTTTTGGCATGCCAGGCCGTCTACCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGGGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcg                                                                                                                                                    <  1:1125153/150‑1 (MQ=255)
    aGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcgg                                                                                                                                                   >  2:855109/1‑150 (MQ=255)
     gCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGcggtcggt                                                                                                                                                  >  2:1195306/1‑150 (MQ=255)
      ccAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCATTCGGTCATCGCCAGTAAACCGTTATGGTGAATACGTGCCATCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTg                                                                                                                                                 <  2:1497657/150‑1 (MQ=255)
       cAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGc                                                                                                                                                >  2:1176620/1‑150 (MQ=255)
        aGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGACCGCCCGCGTCCGGTGcc                                                                                                                                               <  2:1239175/150‑1 (MQ=255)
          aaaGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGt                                                                                                                                             <  1:1756877/150‑1 (MQ=255)
           aaGCGTAGTGCCAATACTTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTc                                                                                                                                            <  2:1162546/150‑1 (MQ=255)
           aaGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTc                                                                                                                                            <  2:1531623/150‑1 (MQ=255)
            aGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGACCGCCCGCGTCCGGTGcctgtt                                                                                                                                           <  1:1351554/150‑5 (MQ=255)
              cGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAAt                                                                                                                                         <  2:1014050/150‑1 (MQ=255)
                  gTGCCAATATTTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTTAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCg                                                                                                                                     <  2:1753438/150‑1 (MQ=255)
                   tGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGa                                                                                                                                    >  2:1465493/1‑150 (MQ=255)
                      cAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATaa                                                                                                                                 <  1:1488741/150‑1 (MQ=255)
                      cAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATaa                                                                                                                                 >  2:1336721/1‑150 (MQ=255)
                       aataatTTCGGCTCCCAGCTTTTGGCACGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAAt                                                                                                                                >  1:457991/1‑150 (MQ=255)
                               cGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCgcgc                                                                                                                        >  2:1704553/1‑150 (MQ=255)
                                     ccAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCt                                                                                                                  >  1:1189974/1‑150 (MQ=255)
                                      cAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTg                                                                                                                 >  2:220158/1‑150 (MQ=255)
                                        gCTTTTGGCATGCCAGGACGTCTTCCTGCTTTTAGCAGTCGGTCATCGCCCGTAAACAGTGATGCTTAATACGTGCCAGCAGCGTTTCTACAGTCACAGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTgcg                                                                                                               <  2:211109/150‑1 (MQ=255)
                                              ggCATGCCATGCCGTCTTCCGGCGGTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgcg                                                                                                         <  1:364247/150‑1 (MQ=255)
                                              ggCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgcg                                                                                                         >  2:207298/1‑150 (MQ=255)
                                              ggCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAgcg                                                                                                         <  2:1299840/150‑1 (MQ=255)
                                               gCATGCCAGCCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAAAGGCGATAATGTCCGCGCCCGCCTGCGCCAgctc                                                                                                        >  2:494473/1‑148 (MQ=255)
                                                cATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCa                                                                                                       >  1:978791/1‑150 (MQ=255)
                                                  tGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATc                                                                                                     <  1:1195306/150‑1 (MQ=255)
                                                  tGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGGGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATc                                                                                                     <  1:951456/150‑1 (MQ=255)
                                                   gCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCa                                                                                                    >  1:1295211/1‑150 (MQ=255)
                                                    ccAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACAGTGATGGTGAATACGTGCCAGCAGCGTTTCCACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCaa                                                                                                   >  1:1039490/1‑150 (MQ=255)
                                                     cAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAAc                                                                                                  >  1:276666/1‑150 (MQ=255)
                                                        gCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATc                                                                                               <  2:307547/150‑1 (MQ=255)
                                                         ccGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCt                                                                                              >  2:1750962/1‑150 (MQ=255)
                                                          cGTCTTCCCGAGTTGAGCAGTCGGTCAACGACAGTAAACATTGATGGATAAAACGTGCCAGCAGCGTTTCAACAGGCTCCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCtt                                                                                             <  2:1174943/150‑1 (MQ=255)
                                                             cTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCaa                                                                                          <  2:1786609/150‑1 (MQ=255)
                                                             cTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCaa                                                                                          <  1:1046185/150‑1 (MQ=255)
                                                              ttCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAat                                                                                         <  2:711499/150‑1 (MQ=255)
                                                               tCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAatc                                                                                        <  1:1058719/150‑2 (MQ=255)
                                                               tCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAata                                                                                        <  1:1058721/150‑1 (MQ=255)
                                                                ccGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAatat                                                                                       >  2:1113031/1‑150 (MQ=255)
                                                                ccGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAatat                                                                                       <  2:1806823/150‑1 (MQ=255)
                                                                 cGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGCTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAAtata                                                                                      >  1:391467/1‑150 (MQ=255)
                                                                        gAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTg                                                                               >  1:986405/1‑150 (MQ=255)
                                                                          gCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGAt                                                                             >  2:568481/1‑150 (MQ=255)
                                                                           cAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATg                                                                            <  2:1667378/150‑1 (MQ=255)
                                                                           cAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATg                                                                            <  2:104494/150‑1 (MQ=255)
                                                                            aGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGGCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGc                                                                           <  1:707497/150‑1 (MQ=255)
                                                                               cGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTa                                                                        >  2:1611535/1‑150 (MQ=255)
                                                                                ggTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTAc                                                                       <  1:455416/150‑1 (MQ=255)
                                                                                     tCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGGCGTGATGCGTACCGgag                                                                  <  1:214613/150‑1 (MQ=255)
                                                                                     tCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGgag                                                                  >  2:434225/1‑150 (MQ=255)
                                                                                     tCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGgag                                                                  >  1:837543/1‑150 (MQ=255)
                                                                                      cGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGgaga                                                                 >  2:1022262/1‑150 (MQ=255)
                                                                                       gCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGaa                                                                >  1:582024/1‑150 (MQ=255)
                                                                                          aGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAAtcc                                                             <  1:1499405/150‑1 (MQ=255)
                                                                                               aCCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAg                                                        >  1:40523/1‑150 (MQ=255)
                                                                                                ccGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGa                                                       >  2:411242/1‑150 (MQ=255)
                                                                                                ccGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGa                                                       >  1:1505214/1‑150 (MQ=255)
                                                                                                 cGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGAt                                                      >  2:1477082/1‑150 (MQ=255)
                                                                                                    gATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGCCGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCCGCGCAGCAACATCTTCAAGATAGGCCGTCATGCGTACCGGAGAATCCTCCAGATcgc                                                   >  1:243826/1‑150 (MQ=255)
                                                                                                         tGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTc                                              <  1:1541425/150‑1 (MQ=255)
                                                                                                            aTACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTcaca                                           <  1:428879/150‑1 (MQ=255)
                                                                                                              aCGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAAt                                         >  2:748339/1‑150 (MQ=255)
                                                                                                                gTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTc                                       <  1:1128065/150‑1 (MQ=255)
                                                                                                                 tGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTcc                                      <  1:1704553/150‑1 (MQ=255)
                                                                                                                 tGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTcc                                      <  2:575487/150‑1 (MQ=255)
                                                                                                                 tGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGGGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTcc                                      >  2:489079/1‑150 (MQ=255)
                                                                                                                  gCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCAAGCGCATCAAAAACTTCAATATAGGCCGAGATGCGAACCGGAGACTCCTCCAGATAGCAATTAACAATTCCa                                     >  2:946027/1‑150 (MQ=255)
                                                                                                                   ccAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCATCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCaa                                    <  1:855109/150‑1 (MQ=255)
                                                                                                                     agcagcGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCCGCGCATCAACATCTGCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGCTCGCGTTTCACAATTCCaata                                  >  2:537883/1‑150 (MQ=255)
                                                                                                                         gcGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCg                              >  2:1806807/1‑150 (MQ=255)
                                                                                                                         gcGTTGCAACAGGCACCGGACGCGGGCGGTCGTTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATAAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCg                              <  2:1060096/150‑1 (MQ=255)
                                                                                                                          cGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCgg                             <  1:1176620/150‑1 (MQ=255)
                                                                                                                           gTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGc                            >  1:771596/1‑150 (MQ=255)
                                                                                                                           gTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGGGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGc                            <  1:1098073/150‑1 (MQ=255)
                                                                                                                            tttCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCa                           <  1:1721151/150‑1 (MQ=255)
                                                                                                                             ttCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCAc                          >  2:70257/1‑150 (MQ=255)
                                                                                                                              tCAACAGGCACCAGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGGGATGCGTACCGGAGAATCCTCCAGATCGCGGTTCACAATTCCAATAATCGGCACg                         >  2:40298/1‑150 (MQ=255)
                                                                                                                              attgacggCACCGACCGCCCGCGTCCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAAGATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCGAATAATCGGCCCg                         >  2:1134139/7‑150 (MQ=255)
                                                                                                                                aaCAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCCTCACCATCTTCAATATAGGCCGTGATGCGGACCGGAGAATCCGCCAGCTCGCGTTTCACAATTCCAATAATCGGCACGcg                       >  1:313214/1‑149 (MQ=255)
                                                                                                                                aaCAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGAAAAAGACCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGACCCGGACAATCCTCCAGATCGCGGTTCACAATTACAATACTCGGCACGcg                       >  2:337589/1‑149 (MQ=255)
                                                                                                                                 gacggCACCGACCGCCCGCGTCCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTc                      >  1:568554/4‑150 (MQ=255)
                                                                                                                                 gacggCACCGACCGCCCGCGTCCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTc                      >  2:813324/4‑150 (MQ=255)
                                                                                                                                 aCAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTc                      >  1:758390/1‑150 (MQ=255)
                                                                                                                                   cggCACCGACCGCCCGCGTCCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTcac                    >  1:1480666/2‑150 (MQ=255)
                                                                                                                                    ggCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCGGCGCATCAACATGGTCAAGATAGGCCGGGATGCGTACCGGAGAAGACTCCAGATCGCGTTTCACATTTCCAATAATCGGCACGCTcacc                   >  1:960278/1‑150 (MQ=255)
                                                                                                                                    ggCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTcacc                   >  2:481267/1‑150 (MQ=255)
                                                                                                                                    ggCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTcacc                   >  2:1240322/1‑150 (MQ=255)
                                                                                                                                    ggCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGCCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAGTCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTcccc                   >  1:1295724/1‑150 (MQ=255)
                                                                                                                                      cACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCTGCGCATCAACATCTTCAATTTAGGCCGTGATGCGTTCCGGAGAATCCTCCAGATCGCGTTACACAATTCCAATAATCGGCACGCTcacccc                 >  2:960359/1‑148 (MQ=255)
                                                                                                                                          ggACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCa             <  1:458935/150‑1 (MQ=255)
                                                                                                                                            aCGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCAcg           <  2:178346/150‑1 (MQ=255)
                                                                                                                                            aCGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCAcg           <  1:195697/150‑1 (MQ=255)
                                                                                                                                                gggcggTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACACCGCACGCGTgg      <  2:927197/150‑1 (MQ=255)
                                                                                                                                                  gcggTCGGTGCCGTCAATTGCGATAATGTACGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTATCGGATAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCACGCGTggg     <  2:354521/150‑2 (MQ=255)
                                                                                                                                                   cggtcggtGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCACGCGTGGCt    <  2:1361699/150‑1 (MQ=255)
                                                                                                                                                    ggtcggtGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCACGCGTGGCtt   >  2:867832/1‑150 (MQ=255)
                                                                                                                                                     gtcggtGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATACCAATAATCGGCACGCTCACCACCGCACGCGTGGCTTg  >  2:409068/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTATAGCCAGAAAGCGTAGTGCCAATAATTTCGGCTCCCAGCTTTTGGCATGCCAGGCCGTCTTCCGGCGTTGAGCAGTCGGTCATCGCCAGTAAACCGTGATGGTGAATACGTGCCAGCAGCGTTTCAACAGGCACCGGACGCGGGCGGTCGGTGCCGTCAATGGCGATAATGTCCGCGCCCGCCTGCGCCAGCGCATCAACATCTTCAATATAGGCCGTGATGCGTACCGGAGAATCCTCCAGATCGCGTTTCACAATTCCAATAATCGGCACGCTCACCACCGCACGCGTGGCTTG  >  NC_000913/3370564‑3370862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: