Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 399,815 C→T 5.2% intergenic (+10/+14) yaiZ → / ← ddlA DUF2754 family putative inner membrane protein/D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913399,8150CT 5.2% 423.3 / 7.0 136intergenic (+10/+14)yaiZ/ddlADUF2754 family putative inner membrane protein/D‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base C (71/57);  new base T (5/2);  total (76/60)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.61e-01

Only 100 of 139 total aligned reads displayed.

ATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCC  >  NC_000913/399666‑399963
                                                                                                                                                     |                                                                                                                                                    
aTGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATc                                                                                                                                                      >  1:1329180/1‑150 (MQ=255)
aTGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATc                                                                                                                                                      >  1:1321817/1‑150 (MQ=255)
aTGGCGTGGGGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGGGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATc                                                                                                                                                      <  2:353316/150‑1 (MQ=255)
  ggCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTTTTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCgg                                                                                                                                                    >  1:186234/1‑150 (MQ=255)
     gtggtgGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCg                                                                                                                                                 >  2:1223530/1‑150 (MQ=255)
         tggGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTaa                                                                                                                                             >  1:678767/1‑150 (MQ=255)
          ggggTGACTGGGATTTGAATCAAACGGTTGTCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCGATAATATTCTTATTGGGCGttttc                                                                                                                                            <  2:878776/150‑4 (MQ=255)
           ggggTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCTGTCTGGAGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTaaaa                                                                                                                                           <  1:1198369/150‑1 (MQ=255)
            ggTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAat                                                                                                                                          >  2:787962/1‑150 (MQ=255)
             gTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAata                                                                                                                                         >  1:1325269/1‑150 (MQ=255)
                    ggATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCTGGCGTTAAAATATTACAtt                                                                                                                                  >  2:73493/1‑150 (MQ=255)
                     gATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACAttt                                                                                                                                 >  2:774518/1‑149 (MQ=255)
                      aTTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATtgt                                                                                                                                <  2:615061/150‑1 (MQ=255)
                        ttGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTgg                                                                                                                              <  2:9259/150‑1 (MQ=255)
                        ttGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTgg                                                                                                                              >  1:437573/1‑150 (MQ=255)
                        atGAACCAAAAGCGTTGCAGACCTATGCCGTCTGTCTGGTGACGTGTGTTATTATTTTCTGGATGGCGGGGTTGATTATTTGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTgg                                                                                                                              <  2:1190896/149‑1 (MQ=255)
                         tGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCGGGGGACGTGGGGGAGTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGCAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGt                                                                                                                             >  1:1076410/1‑150 (MQ=255)
                          gAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGtt                                                                                                                            >  1:1699098/1‑150 (MQ=255)
                           aaGCAAAAGGTTTGCAGACCTATGCCGTCGGGCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGttt                                                                                                                           <  2:344099/150‑1 (MQ=255)
                            aGCTAAAGGTTGGCAGTCCTATGCGGTCGGTCTGGTGACGGGGGTGTTTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGGCGCGATGCAGAAACTGAAAACGCCCAATATGAATATTATCGGTCGTTAAAATATTACATTGTGGtttt                                                                                                                          <  2:1176215/150‑1 (MQ=255)
                             gCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTc                                                                                                                         >  2:372559/1‑150 (MQ=255)
                             gCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTc                                                                                                                         >  1:1607283/1‑150 (MQ=255)
                              cAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCa                                                                                                                        >  2:943499/1‑150 (MQ=255)
                               aaaaGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCaa                                                                                                                       >  2:1277439/1‑150 (MQ=255)
                               aaaaGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCaa                                                                                                                       >  2:1688510/1‑150 (MQ=255)
                               aaaaGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCaa                                                                                                                       >  2:1058338/1‑150 (MQ=255)
                               aaaaGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCaa                                                                                                                       >  1:677872/1‑150 (MQ=255)
                               aaaaCGTTGGCAGACCTTTGCCGTCGGTCGGGTGACGTGGGTGATTAGTTTGTGGCTGGCGGGGTTGAATATTCGTCGTCGCGATGATCAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATAATACAATGTGCTTTTCaa                                                                                                                       >  2:451720/1‑150 (MQ=255)
                                    gTTGTCAGACCTATGCCGTCGGTCTGGTGTCGTGGTTGATTAGTTTCTTGCTGGCGGGGTTGATTATTCGTCGTCGCTATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGt                                                                                                                  <  1:1183487/150‑1 (MQ=255)
                                    gTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGt                                                                                                                  >  1:454423/1‑150 (MQ=255)
                                    gTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGt                                                                                                                  <  1:550511/150‑1 (MQ=255)
                                     gtGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGtt                                                                                                                 <  2:1566624/149‑1 (MQ=255)
                                        gCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATc                                                                                                              >  1:1389629/1‑150 (MQ=255)
                                          aGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTACAATGCGTTATCCg                                                                                                            >  2:306743/1‑150 (MQ=255)
                                           gACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTATCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGc                                                                                                           >  1:303875/1‑150 (MQ=255)
                                            aCCTATGCCGTCTGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCa                                                                                                          <  1:1474143/150‑1 (MQ=255)
                                            aCCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGTCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCa                                                                                                          <  2:1811899/150‑1 (MQ=255)
                                            aCCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCa                                                                                                          <  1:1596928/150‑1 (MQ=255)
                                               tATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACTCCCAATAAGAATATTATCGGTCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCg                                                                                                       >  2:287690/1‑150 (MQ=255)
                                                aTGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGACCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCTCAGCGt                                                                                                      >  2:1791081/1‑150 (MQ=255)
                                                      tCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCt                                                                                                <  1:1727005/150‑1 (MQ=255)
                                                       cGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTc                                                                                               <  1:577736/150‑1 (MQ=255)
                                                        ggTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTcc                                                                                              >  1:1238358/1‑150 (MQ=255)
                                                           cttgttaCGTGGGTGATTAGTTTCTGGCTGTCGGGGTTGATTATTCGTCGTATCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCTGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAgc                                                                                           <  2:594886/144‑1 (MQ=255)
                                                             ggTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAgcgc                                                                                         >  1:1422725/1‑150 (MQ=255)
                                                              gTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGGGTTAAAATATTACATTGTGGTTTTCAATGCGTTATACGAAGCGTGACGCTCCAGCGcc                                                                                        >  1:1821671/1‑150 (MQ=255)
                                                                gACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAGTGCGTTATCCGCAGCGTGACGCTCCAGCGCCAg                                                                                      <  2:1485706/150‑1 (MQ=255)
                                                                  cGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGtt                                                                                    <  1:816755/150‑1 (MQ=255)
                                                                   gTGGGTGATTAGTTTCTGGCTGGCTGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtc                                                                                   <  1:780121/150‑1 (MQ=255)
                                                                     gggTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAAAAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaa                                                                                 <  1:1198186/150‑1 (MQ=255)
                                                                        tGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTtcaatca                                                                              <  1:1223530/150‑1 (MQ=255)
                                                                           ttAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAAc                                                                           <  2:678767/150‑1 (MQ=255)
                                                                           ttAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAAc                                                                           <  2:1793127/150‑1 (MQ=255)
                                                                                 tCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGa                                                                     <  2:551563/150‑1 (MQ=255)
                                                                                      ctggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGAGATCAGa                                                                >  1:1716255/1‑150 (MQ=255)
                                                                                       tggcGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGAt                                                               >  2:293217/1‑150 (MQ=255)
                                                                                          cGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCgg                                                            <  1:787962/150‑1 (MQ=255)
                                                                                             ggTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGgtgt                                                         >  1:579336/1‑150 (MQ=255)
                                                                                              gTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTa                                                        <  1:1033125/150‑1 (MQ=255)
                                                                                              gTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTa                                                        >  1:249326/1‑150 (MQ=255)
                                                                                                tGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAAc                                                      <  2:1245767/150‑1 (MQ=255)
                                                                                                 tattattCGTCTTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCCGCGCCAGTTCAATCAAACGTGTGATCAGATCGTTGTAAcc                                                     >  1:287368/2‑150 (MQ=255)
                                                                                                   taattCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCa                                                   >  2:671489/3‑150 (MQ=255)
                                                                                                      ttCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCATCGTGACTCTCCATCGCCAGTTCAATCAAACGTGTGATCATATCGGTGTAACCCAGAc                                                >  2:1174737/1‑150 (MQ=255)
                                                                                                      ttCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGAc                                                <  1:1589654/150‑1 (MQ=255)
                                                                                                      ttCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGAc                                                <  1:797859/150‑1 (MQ=255)
                                                                                                            gtcgCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTgg                                          >  2:182411/1‑150 (MQ=255)
                                                                                                            gtcgCGATGAAAAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCATCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTgg                                          >  1:1459395/1‑150 (MQ=255)
                                                                                                             tcgCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGc                                         <  2:1026938/150‑1 (MQ=255)
                                                                                                              cgcgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCt                                        <  2:78087/150‑1 (MQ=255)
                                                                                                                cgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTg                                      <  2:186234/150‑1 (MQ=255)
                                                                                                                cgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTg                                      >  2:1723129/1‑150 (MQ=255)
                                                                                                                cgATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTg                                      <  1:1563080/150‑1 (MQ=255)
                                                                                                                 gATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGc                                     <  1:36604/150‑1 (MQ=255)
                                                                                                                  aTGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGcc                                    <  1:34797/150‑1 (MQ=255)
                                                                                                                  aTGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGcc                                    <  1:966346/150‑1 (MQ=255)
                                                                                                                    gaagaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCcac                                  >  2:584196/1‑150 (MQ=255)
                                                                                                                      agaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCTCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAg                                >  2:883173/1‑150 (MQ=255)
                                                                                                                      agaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACACAGACCGCTGTCTTCCCACAg                                >  1:88660/1‑150 (MQ=255)
                                                                                                                       gaaACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGc                               <  1:289474/150‑1 (MQ=255)
                                                                                                                           cTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCg                           <  2:1607283/150‑1 (MQ=255)
                                                                                                                            ttttaaCGCCCGATAATAATCTTATTGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTTCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCgg                          >  2:751197/5‑150 (MQ=255)
                                                                                                                             gAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACATCTTCGGa                         >  2:1119233/1‑150 (MQ=255)
                                                                                                                             gAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGa                         <  2:1407654/150‑1 (MQ=255)
                                                                                                                             gAAAACGCCCAATAAGAAGATTATCGGGCGTGAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTATCCCCGACCGCTGGCTTGCCCCAGCTTCGGa                         >  1:1635025/1‑150 (MQ=255)
                                                                                                                              ttaaCGCCCGATAATATTCTTATTGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCTATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGat                        >  2:1314584/3‑150 (MQ=255)
                                                                                                                              aaaaCGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGat                        <  2:755897/150‑1 (MQ=255)
                                                                                                                              aaaaCGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGat                        <  2:1321817/150‑1 (MQ=255)
                                                                                                                              aaaaCGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGGGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGnt                        <  2:1329180/150‑1 (MQ=255)
                                                                                                                               aaaCGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCCGACCGCTGGCTTGCCACAGCTTCGGata                       >  1:208150/1‑150 (MQ=255)
                                                                                                                                aaCGCCCGATAATATTCTTATTGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatac                      >  2:364332/1‑150 (MQ=255)
                                                                                                                                   gCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatacata                   >  2:1139593/1‑150 (MQ=255)
                                                                                                                                    cccAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGatacatac                  >  1:1088543/1‑150 (MQ=255)
                                                                                                                                      cAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTg                <  2:1389629/150‑1 (MQ=255)
                                                                                                                                       aaTAAGAATATTATGGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGa               <  2:231909/150‑1 (MQ=255)
                                                                                                                                              atatTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAAAGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGt        <  1:306743/150‑1 (MQ=255)
                                                                                                                                               tattatCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTg       >  1:1455707/1‑150 (MQ=255)
                                                                                                                                                 ttatCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTGAAGCGCCAGTTCAATCAAACGTGAGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGaa     >  1:1485898/1‑150 (MQ=255)
                                                                                                                                                   atCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGc   >  1:1395962/1‑150 (MQ=255)
                                                                                                                                                    tCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGcc  <  1:566261/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATGGCGTGGTGGGGTTACTGGGATTTGAAGCAAAAGGTTGGCAGACCTATGCCGTCGGTCTGGTGACGTGGGTGATTAGTTTCTGGCTGGCGGGGTTGATTATTCGTCGTCGCGATGAAGAAACTGAAAACGCCCAATAAGAATATTATCGGGCGTTAAAATATTACATTGTGGTTTTCAATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCC  >  NC_000913/399666‑399963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: