Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,392,189 T→G 5.1% intergenic (‑161/+269) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,392,1890TG 5.1% 297.5 / 3.3 126intergenic (‑161/+269)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base T (52/65);  new base G (4/4);  total (57/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGC  >  NC_000913/3392041‑3392335
                                                                                                                                                    |                                                                                                                                                  
tCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGGGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAGGACTCACAGCGTCGCATCAGGAAACGGCTTTCGGATGCGGCGTAAACGCCtg                                                                                                                                                   <  2:295800/150‑2 (MQ=255)
        taCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                           <  2:469558/150‑1 (MQ=255)
         aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATAGGGCGTTCACGCCGCATCCggcagc                                                                                                                                         <  1:1816946/150‑6 (MQ=11)
          cTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCCGCCTACGGTTATGTTCAGGTTTGTAGGCGTGATAAGACGCACAGCGTCGCATAAGGCACCGGCTGTAGGATGCGGCGTAAACGCCTTATcctaccta                                                                                                                                         >  1:1780483/1‑150 (MQ=18)
          cTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATAAGGCGTTTACGCCGCATCCGAcagc                                                                                                                                         <  2:1453545/150‑4 (MQ=18)
          cTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCACCCTACGGTTATGTTCAGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCACCGGATGACGGATGCGGCCTAAACACCTTAACCGACCTa                                                                                                                                         >  2:642319/1‑150 (MQ=18)
           tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAc                                                                                                                                        <  1:1237286/150‑1 (MQ=255)
            gCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCCGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACg                                                                                                                                       >  1:200417/1‑150 (MQ=255)
            gCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCATATCCAGCCTACGGTTATGTTCCGTTTTGTAGGCATAAAAAAACGCACACCGTAGCATCATGAAACGAATTTCGGATACGTCGTAAACGCCTAATCCTACATAca                                                                                                                                       >  2:153921/1‑149 (MQ=18)
             cAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                                                                                                      <  2:144856/150‑1 (MQ=255)
              aGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                                                                                     <  2:1063430/150‑1 (MQ=255)
              aGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCGGATAAGACGCACAGCGTCGCATCAGGCAACGGCGGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                                                                                     <  2:74384/150‑1 (MQ=255)
                 agagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                                                                                                                  <  2:973004/150‑1 (MQ=255)
                  gagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATg                                                                                                                                 <  1:1211824/150‑1 (MQ=255)
                  caggccgttttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTCGGCCTCATAAGCCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATg                                                                                                                                 <  2:280222/143‑1 (MQ=17)
                  cagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATg                                                                                                                                 <  1:112554/149‑1 (MQ=255)
                   agGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGt                                                                                                                                <  2:1303932/150‑1 (MQ=255)
                    ggATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGtt                                                                                                                               >  1:1763649/1‑150 (MQ=255)
                    ggATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGAGTAAACGCCTTATCCGACCTACGGTTATGtt                                                                                                                               >  1:1731520/1‑150 (MQ=255)
                    agATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGTTTTGTAGGCATGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACGGATGCGGCGTAAACGCCTTATCCGACCTACGGATATgat                                                                                                                               >  1:743592/2‑148 (MQ=17)
                     gATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTCTCGGATGCGGCGTAAACGCCCTATCCGACCTACGGTTATGTTc                                                                                                                              >  2:29821/1‑150 (MQ=255)
                      aTTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                                                                                                             <  1:833743/150‑1 (MQ=255)
                         ttttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGtt                                                                                                                          >  1:842031/1‑150 (MQ=255)
                             aCGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTa                                                                                                                      <  1:1267130/150‑1 (MQ=255)
                               ggCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAgg                                                                                                                    <  1:237246/150‑1 (MQ=255)
                                gcggCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTAGGTTCCGGTGTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                                                                                                                   <  1:110503/147‑1 (MQ=255)
                                 cTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGcc                                                                                                                  <  1:39349/150‑1 (MQ=255)
                                  tGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                 <  2:113668/150‑1 (MQ=255)
                                   gCCTGATGCGGCGTGAACGCCCTATCCAGCCTTCGGTTTTGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAATCGCCTTATCCGACCTACTGTTATGTTCCGTTTGTAGGCCTg                                                                                                                <  1:282520/150‑1 (MQ=14)
                                   gCCGTATGCGGCGTGAACGCCTTATCCAGCCTACGGTAATGTTCCGGTTTGTCGGCCTGATACGAGGCACAGGGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTg                                                                                                                <  1:1668075/150‑1 (MQ=14)
                                   gCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTg                                                                                                                >  1:1534477/1‑150 (MQ=255)
                                      ggttGCGGCGTGAACGGCCTATCTTGCCTTCTGTTTTGTCCCGGTTTGTCGGACTGTTAAGACTCACATCGAGCGTTCAGGGAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATa                                                                                                             <  1:289294/147‑1 (MQ=14)
                                          gcggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGAc                                                                                                         <  1:1047803/150‑1 (MQ=255)
                                            ggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGAAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGc                                                                                                       <  1:372542/150‑1 (MQ=255)
                                              cgTGAACTAGCTATCCAGCCTACGGGGCTGCGCCGGTCTGGCGGCCGGATACGCCGCACCGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAACCGCCGTATCCGACCTACGGTTAGGTTCCGTCTGTAGGCCTGATAAGACGcac                                                                                                     <  2:291560/150‑1 (MQ=9)
                                              cgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAATCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTTATAAGACGcac                                                                                                     >  2:544004/1‑150 (MQ=255)
                                              cgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcac                                                                                                     <  1:1467729/150‑1 (MQ=255)
                                              cgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAAGGTTATGTTCCGTATGTAGGCCTGATAAGACGcac                                                                                                     >  2:551199/1‑150 (MQ=255)
                                                 gAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGc                                                                                                  >  2:198133/1‑150 (MQ=255)
                                                    cGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                               >  2:692288/1‑150 (MQ=255)
                                                     gCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg                                                                                              >  1:439816/1‑150 (MQ=255)
                                                       ccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCa                                                                                            >  1:1019803/1‑150 (MQ=255)
                                                       ccTATCCAGCCTACGGTTATGTTCAGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACTCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATACGACGCACACCCTCGCa                                                                                            >  2:1617889/1‑150 (MQ=14)
                                                        cTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGTTTGTAGGCCTGATAATACTCACAGCGTCGAAt                                                                                           >  2:713643/1‑150 (MQ=255)
                                                          aaccAGCCTACGGTTCTGTCCCGGTTGGCAGGCCTGTTAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                                         <  1:1386731/148‑1 (MQ=14)
                                                          aTCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                                         <  2:360254/150‑1 (MQ=255)
                                                           tCCAGCCTACGTTTCTGTACAGGTTTGGCGGCCTGATTAGTCGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                                        <  1:278442/150‑1 (MQ=14)
                                                            ccAGCCTACGGTTATGTTCCGTTTTGATGGCCTGATAAGACGCACACGGTCGCATCAGTCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                                                                       <  1:364542/150‑1 (MQ=11)
                                                            ccAGCCTACGGTTATGTTCCGGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                                                                       <  1:1525265/150‑1 (MQ=255)
                                                            ccAGACTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                                                                       <  1:1037038/150‑1 (MQ=38)
                                                                 cTACGGATTTGTGCCGGTTTGTAGGCCTGCTTAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACg                                                                                  <  1:342519/150‑1 (MQ=11)
                                                                   aCGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTATGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGc                                                                                >  2:1321087/1‑150 (MQ=37)
                                                                   aCGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGTCCGGATAAGACGCACAGCGTCGCAACAGGCAACGGc                                                                                >  2:304171/1‑150 (MQ=255)
                                                                    cGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCt                                                                               >  2:1462608/1‑150 (MQ=37)
                                                                    cGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTGTGTAGGCCTGATACGACGCACAGCGTCGCATCAGGCAACGTCt                                                                               >  2:950466/1‑150 (MQ=255)
                                                                       ttAGGTTCCGGTTTGTAGCCCTGATATTCCGCACAGCGGCGCATCAGGCAACGGCTGGCGGAGGCGGCGTAAACGCCTTACCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGcc                                                                            <  2:1316713/150‑1 (MQ=11)
                                                                         ttGTTCCGGTTTTTCGGCCTTTTAAGACCCACAGCGTCGCTTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGGGTCGAATCAGGCAACGGCTGCCgg                                                                          <  2:564991/149‑1 (MQ=11)
                                                                           gTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAt                                                                        >  1:1535039/1‑150 (MQ=37)
                                                                           gTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGAACTACGGTTATGTTCCGTTTGTAGGCCTGATAAGAAGCACAGCGTAGCATCAGGCAACGGCTGCCGGAt                                                                        >  1:576876/1‑150 (MQ=255)
                                                                            ttCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGTTTGTAGGCCTGATAAGACCCACAGAGTCGCCTCAGGCAACGGCTGCCGGATg                                                                       >  1:918355/1‑150 (MQ=255)
                                                                             tCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAACCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgc                                                                      >  1:1141894/1‑150 (MQ=25)
                                                                              ccGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcg                                                                     >  1:1004520/1‑150 (MQ=34)
                                                                               gggTTTGTAGGCCTTATAAGACGCACTGCGTCGAATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAGGTTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcgg                                                                    <  2:372018/149‑1 (MQ=11)
                                                                                ggTTTGTAGGCCTGATATGACTCACAGCGTCGAATGAGTAAAGGGCTGTCGTTTGCGGCGTAAACGCGTTATCCGACCTACGGTTATGTTCCGTTTGTTGGCCTGTTAAGACGCACAGCGTCGCATCAGGAAACGGCTGCCGGATgcggc                                                                   <  2:1556738/150‑1 (MQ=11)
                                                                                 gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggcg                                                                  >  2:1763565/1‑150 (MQ=32)
                                                                                  tttGTAGGCCTGATAAGACGCACAGCGTCTCATCAGGCAACGTCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCCGGCAGCCGTTGCCTGATgcgacgc                                                                 <  1:1078372/150‑2 (MQ=11)
                                                                                    tGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGGTCTAGGCCTGATAAGACGCACAGCGTCGCCTCAGGCAACGGCTGCCGGATGCGGCGTaa                                                               >  1:278728/1‑150 (MQ=255)
                                                                                        ggCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGc                                                           >  2:398522/1‑150 (MQ=34)
                                                                                          ccTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGccc                                                         >  1:47436/1‑149 (MQ=18)
                                                                                          ccTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCt                                                         >  2:1178507/1‑150 (MQ=35)
                                                                                          ccTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCt                                                         >  1:1523739/1‑150 (MQ=35)
                                                                                           cTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTg                                                                                                                >  1:1642414/1‑94 (MQ=33)
                                                                                           cTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTg                                                                                                                <  2:1642414/94‑1 (MQ=33)
                                                                                               aaaGACGCACAGCGTCGATTAAGTCAAGGGCTGTCGTTTGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATcc                                                    <  2:1753459/149‑1 (MQ=11)
                                                                                                   aCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAcc                                                >  2:1699187/1‑150 (MQ=37)
                                                                                                      cacaGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGACTGCCGGATGCGGCGTAAACGCCCTATCCGACCTAc                                             >  1:1224739/1‑150 (MQ=17)
                                                                                                       acaGCGTAGAATCTGGAAAGGGCTTTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCTTCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACg                                            <  2:1731520/150‑1 (MQ=11)
                                                                                                        caGCGTCGCATCTGGCAACGGCTGTCGTATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                           <  1:1805206/150‑1 (MQ=255)
                                                                                                           cGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTa                                        <  2:1287877/150‑1 (MQ=37)
                                                                                                            gTCGCATCAGGAAACGGGTGGCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                       <  1:1607918/150‑1 (MQ=255)
                                                                                                                  tctggCAACGGCGGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCg                                 <  1:692840/147‑1 (MQ=255)
                                                                                                                   cAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTATCTAGGCCTGATAAGACGCACAGCGTCGCATCAGTCAACGGCTGCCGGATGCGGCGTAAAAGCATAATCCGACCTACGGTTATGTTCAGt                                >  2:1467105/1‑150 (MQ=11)
                                                                                                                    tgggaaCGGCTGTCGTATGCGGATTAAATGCTTTATACGACATAGGTTTATGTTCCGTTTGTAGGCCTGTTAAGACGCACAGCGTCTCATAAGGCTACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGtt                               <  2:360786/146‑1 (MQ=14)
                                                                                                                    aGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGTTTGTAGGCCTGATAAGACGCCCAGCGTCGCCTCAGGCAAATGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGtt                               >  2:173669/1‑150 (MQ=11)
                                                                                                                       aaaCGGCTGTCGGCGGCGGCGTAAACGCCTTTTCCGACATAGGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGATTCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGt                            <  1:1314375/149‑1 (MQ=14)
                                                                                                                        aaCGGCGGTCGGCTGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCAGAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTa                           <  2:1733314/150‑1 (MQ=255)
                                                                                                                         aCGTCTGTGGGAGGCGGCGTAAACGCCTTATCCGACCTAGGGTTATGTTCCGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAg                          <  1:489994/150‑1 (MQ=14)
                                                                                                                          cGGCTGTCGGATGCGGCGTAAACGCATTATCCGACCTACGGTTATGTTCGGGTTGTAGGCCTGATAAGACGCACAGCGACGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCGTATCCGACCTAGGGTTATGTTCCGTTTggtgg                         >  2:272701/1‑146 (MQ=14)
                                                                                                                            gCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTCCGGTTATGTTCCGTGTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCCCCGGCTGCCGGATGCGGCGTAACCGCCATATCCTACCGACGGTGATGTTCCGGTTGGAGGcc                       >  2:930917/1‑150 (MQ=14)
                                                                                                                             cTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                      <  1:680363/150‑1 (MQ=255)
                                                                                                                               gTCGGATAAGGCGTTTACGCCGCATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACTGTTATGTTCGGGTTGTTGGcagga                    >  2:1743874/1‑146 (MQ=11)
                                                                                                                                 cGGATAAGGCGTTTACGCCGCATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATa                  >  2:1311348/1‑150 (MQ=14)
                                                                                                                                  ggataaggcgTTTACGCCGCATCCGCCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGGAAACGCCGTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATaa                 >  1:1579024/7‑150 (MQ=11)
                                                                                                                                  ggagggggcgTAAACGCGTTATCTGCCCTCGGTTTATTTTCGGTTTGTAGGCGTGATAAGACGCACAGGGTCGCATATGTCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa                 <  2:1652082/144‑1 (MQ=14)
                                                                                                                                       cggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGc            >  2:488034/1‑150 (MQ=255)
                                                                                                                                              aaCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGTTTGTAGGCCTGATAAGACGCACAGCGt     >  2:28037/1‑150 (MQ=255)
                                                                                                                                               aCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc    <  2:534794/150‑1 (MQ=255)
                                                                                                                                                cGCCTAATCCGACTTACGGTTTTTTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg   <  2:1763649/150‑1 (MQ=255)
                                                                                                                                                 gCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc  >  2:1642230/1‑150 (MQ=255)
                                                                                                                                                 gCCTTATCCGACCTACGGTTATGTTCAGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAAGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTAAGGTTATGTTAAGTTTGTAGGCCTGATAAGACGCACAGCGTCGc  >  2:452514/1‑150 (MQ=14)
                                                                                                                                                    |                                                                                                                                                  
TCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGC  >  NC_000913/3392041‑3392335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: