Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,392,320 A→C 5.5% intergenic (‑292/+138) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,392,3200AC 5.5% 231.2 / 4.1 96intergenic (‑292/+138)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base A (57/33);  new base C (2/4);  total (59/37)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 101 total aligned reads displayed.

GGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTT  >  NC_000913/3392171‑3392469
                                                                                                                                                     |                                                                                                                                                     
ggataaggcgTTTACGCCGCATCCGCCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGGAAACGCCGTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATaa                                                                                                                                                       >  1:1579024/7‑150 (MQ=11)
ggagggggcgTAAACGCGTTATCTGCCCTCGGTTTATTTTCGGTTTGTAGGCGTGATAAGACGCACAGGGTCGCATATGTCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa                                                                                                                                                       <  2:1652082/144‑1 (MQ=14)
    gcggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACg                                                                                                                                                   >  2:491938/1‑150 (MQ=255)
     cggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGc                                                                                                                                                  >  2:488034/1‑150 (MQ=255)
     cggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGACATATCCGACCTACTGTTATGTTCAGGATGTAGGCCGTTTAAGAccc                                                                                                                                                  >  1:1176611/1‑148 (MQ=11)
      ggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCCTATGTAGGCCTGATAAGACGca                                                                                                                                                 >  2:1293125/1‑150 (MQ=255)
      ggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTCTAGGCCTGATAAGACGCACAGCGTCGCATCAGTCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTATGTAGGCCTGATAAGACGca                                                                                                                                                 >  2:570039/1‑150 (MQ=255)
            aaCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGTTTGTAGGCCTGATAAGACGCACAGCGt                                                                                                                                           >  2:28037/1‑150 (MQ=255)
             aCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                                                                          <  2:534794/150‑1 (MQ=255)
              cGCCTAATCCGACTTACGGTTTTTTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg                                                                                                                                         <  2:1763649/150‑1 (MQ=255)
               gCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                                                                                                                        >  2:1642230/1‑150 (MQ=255)
               gCCTTATCCGACCTACGGTTATGTTCAGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAAGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTAAGGTTATGTTAAGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                                                                                                                        >  2:452514/1‑150 (MQ=14)
                    aTCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCGGGTTGTAGGCCGGATCAGACGCACAGCGACGCAACAg                                                                                                                                   >  1:22615/1‑150 (MQ=14)
                     tCCGACCTACGGTTATGTTCCGTTTGTAGTCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                                                                                                                  <  2:474835/150‑1 (MQ=255)
                      ccGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAGGc                                                                                                                                 <  2:753121/150‑1 (MQ=14)
                      ccGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGc                                                                                                                                 <  2:430927/150‑1 (MQ=39)
                      ccGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCATCGCATCAGGc                                                                                                                                 >  2:1557706/1‑150 (MQ=255)
                       cGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGcc                                                                                                                                >  2:1572841/1‑149 (MQ=38)
                        gACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAGGcct                                                                                                                               <  1:446016/150‑3 (MQ=11)
                        gACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCaa                                                                                                                               >  2:1422512/1‑150 (MQ=38)
                             aCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCAGGTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCt                                                                                                                          >  1:921231/1‑150 (MQ=255)
                              cGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACCGCTg                                                                                                                         >  1:927841/1‑150 (MQ=25)
                                ttttttttCGGTTTGTTGGCTTTATATGACGCACAGGGTGGCATTTGGAAACGGCTGCCGGTTTCGGCGTAAACGCCTTATCCGACCTACGGTTTTTTTCCGTTTGTAGGCCTTATAAGACGCACTGCGTCGCTTATGGAAACGGCTGcc                                                                                                                       <  2:743592/144‑1 (MQ=2)
                                     gTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATg                                                                                                                  >  2:1007668/1‑150 (MQ=35)
                                       tCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcg                                                                                                                >  1:1068679/1‑150 (MQ=34)
                                            ttGTAGGCTTCTTAAGACGCACAGCGTCGCATCTGGCAACGGCTGCGGTTTGCGGCTTAAACGCCTTATCCGACCTACGTTTATTATCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTg                                                                                                           <  2:576876/150‑1 (MQ=11)
                                                   ccTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGccc                                                                                                    >  1:1007600/1‑150 (MQ=35)
                                                     tGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTa                                                                                                  >  1:853906/1‑150 (MQ=37)
                                                        tAAGACGCACAGCGTCGCTTTATTCAACGGATGACGGATGGTGCGTAAACGCCTTTTCTGACATAGGTTTATTTTCCGTTTGTAGGCCTGATAAGACTCACATGGTGTCTTCTGGAAACGGCTGCCGGATGCGGCGTGAACGCCCTATcc                                                                                               <  2:650185/150‑1 (MQ=2)
                                                         aaGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCg                                                                                              >  2:1541748/1‑150 (MQ=37)
                                                             cGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATAAGGCGTTTACGCCGCATCCggcag                                                                                          <  1:1276007/150‑5 (MQ=11)
                                                               cacaGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTAc                                                                                        >  2:730591/1‑150 (MQ=37)
                                                                     gTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTAt                                                                                  >  1:1821335/1‑150 (MQ=37)
                                                                      tCGCATCAGGCAACGGCTGCCGTATGCGGCGTAAACACCATATCCTACCTACGGTAATGTTCCCTATGTAGGCCTGATAAGACGCACAGCCTCGCATCATGCAACTGCTGCCTGATGCTGCGTCACAGACCTAACCGACATACGCTTATg                                                                                 >  1:1209208/1‑150 (MQ=11)
                                                                      ccGCATCAGGCAACGGCTGCCGGATGCGGCGGAAACGCCTTATCCGACATAGGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCTTCAGGAAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATg                                                                                 <  2:1780417/149‑1 (MQ=11)
                                                                        gCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGtt                                                                               >  1:1402851/1‑150 (MQ=37)
                                                                        gCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGACCTATCCGACCTACGGTTATGtt                                                                               >  2:1183677/1‑150 (MQ=37)
                                                                         cATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTc                                                                              >  1:1008582/1‑150 (MQ=37)
                                                                                cAAGGGCTGCCGTATGCGGCGTAAACGCCTTATCCGACCTACGGTTATTTTCCGTTTGTTGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCg                                                                       <  1:591489/150‑1 (MQ=14)
                                                                                   cGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCGCTATCCGACGTACGGTGAGGTTCTGGCTCGTTg                                                                    >  1:1340765/1‑150 (MQ=255)
                                                                                   cGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGGTAGGTTCTGGCTCGTTg                                                                    >  1:668644/1‑150 (MQ=255)
                                                                                     gttGCCTGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGc                                                                  >  1:477979/3‑150 (MQ=255)
                                                                                          cGGATGCGGCGTAAACTCCTTATCCGACCTCCGGGTATGTTCCGGTTGTAGGCCTGATCAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                             <  1:272701/150‑1 (MQ=17)
                                                                                          cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                             <  1:452514/150‑1 (MQ=255)
                                                                                          cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                             <  1:1763565/150‑1 (MQ=255)
                                                                                           ggATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGGGTGGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTgg                                                            <  2:1811011/150‑1 (MQ=255)
                                                                                           ggATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTgg                                                            <  1:713643/150‑1 (MQ=255)
                                                                                           ggAGGCGGCGTAAACGCCTTATCCGCCCTACGGTTATGCGCCGTTTGTAGGCCTGATCAGACGCACAGCGGCGCTTCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTgg                                                            <  1:173669/150‑1 (MQ=17)
                                                                                            gATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGc                                                           <  2:477979/150‑1 (MQ=255)
                                                                                              tGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCaa                                                         >  2:1358197/1‑150 (MQ=255)
                                                                                                 ggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGa                                                      >  2:604008/1‑150 (MQ=255)
                                                                                                 ggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGACTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGa                                                      >  2:607386/1‑150 (MQ=255)
                                                                                                  ggtcaaaCGCCTTAGCCGACCTACGTTTAGGTTCCGGTTGTCGCCCTGATACTACGCCCAGCGTCGCTTCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTGCTGGCTCGTTGGCCTTTGGCAACGAt                                                     <  1:930917/146‑1 (MQ=17)
                                                                                                   cgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGCCCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGAtt                                                    >  2:121724/1‑150 (MQ=255)
                                                                                                    gTAAACGCCTTATCCGACCTACGGTTATTTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTa                                                   <  2:842031/150‑1 (MQ=255)
                                                                                                    gTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTa                                                   <  1:198133/150‑1 (MQ=255)
                                                                                                      aaaCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGTCCTTTGGCAACGGGTATc                                                 >  2:865870/1‑150 (MQ=255)
                                                                                                      aaaCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATc                                                 >  2:815604/1‑150 (MQ=255)
                                                                                                        aCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCTACGATTATCCt                                               >  2:392364/1‑150 (MQ=255)
                                                                                                         cGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTa                                              <  1:488034/150‑1 (MQ=255)
                                                                                                          gCCTTAACCGACCTACGGTTATGTTCCTTTTGTAGGCCTGATACGACCCACAGCGTCGCATCAGGAAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTAt                                             <  1:1617889/150‑1 (MQ=18)
                                                                                                           ccTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATg                                            >  2:1710250/1‑150 (MQ=255)
                                                                                                           cattATTCGACTTACGGTTTTGTTTCGGTTGTTGGACTGTTTAGTCTCAGATGGTGTCTTCTTGTAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATg                                            <  2:174370/148‑1 (MQ=16)
                                                                                                            cTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATga                                           >  1:1639409/1‑150 (MQ=255)
                                                                                                                 ccGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTg                                      >  1:471296/1‑150 (MQ=255)
                                                                                                                  cGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGTCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTgg                                     >  2:151215/1‑150 (MQ=255)
                                                                                                                  cGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTgg                                     >  2:1074428/1‑150 (MQ=255)
                                                                                                                    cccTACGGTTATGCTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTgggg                                   <  1:1467105/149‑1 (MQ=255)
                                                                                                                    aCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAATGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTgggg                                   <  2:1019803/150‑1 (MQ=255)
                                                                                                                    aCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTgggg                                   <  2:47436/150‑1 (MQ=255)
                                                                                                                    aCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCATCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTgggg                                   <  1:1557706/150‑1 (MQ=255)
                                                                                                                     ccTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGc                                  <  2:588377/150‑1 (MQ=255)
                                                                                                                     ccTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGc                                  <  2:1534477/150‑1 (MQ=255)
                                                                                                                     ccTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGGTATGTTCTGGCTCGTTGGCCTTGGGCACCGAGTATCCTATGAGATTGGGGc                                  >  2:348608/1‑150 (MQ=255)
                                                                                                                     ccTACGGTTATGTTCCGTTTGTAGGCCGGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGc                                  <  2:1060429/150‑1 (MQ=255)
                                                                                                                      ccaCGTTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGACGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCa                                 <  1:385436/148‑1 (MQ=255)
                                                                                                                      cTACGGAGATGTGCCGTATGTAGGCGTGTTAAGACGCACAGCGTCTGATCAGGAAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGct                                 <  1:385439/150‑2 (MQ=21)
                                                                                                                        aCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACTCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAAt                               >  1:1220698/1‑150 (MQ=255)
                                                                                                                         cGGTTATTTTCCGTTTTTAGTCCTGATAAGTCTCACAGCGTCGCATCTGGAAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAAtt                              <  2:1094092/150‑1 (MQ=21)
                                                                                                                          ggTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTa                             >  1:1366353/1‑150 (MQ=255)
                                                                                                                           gTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTAc                            >  1:890346/1‑150 (MQ=255)
                                                                                                                               tGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGTCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACgcgc                        >  1:1275268/1‑150 (MQ=255)
                                                                                                                                gTTCCGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCCGCCGGATGCGGCGTGAACGCCCTATCCTACCTACGTTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAAATTGGGGCAATTACGCGcc                       >  1:1280577/1‑150 (MQ=25)
                                                                                                                                gTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGcc                       >  2:1260714/1‑150 (MQ=255)
                                                                                                                                gTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGATCGTTGGCCTTTGGCAACGATTATCCTATGACATTGGGGCAATTACGCGcc                       >  1:508545/1‑150 (MQ=255)
                                                                                                                                  tCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGCCCTACGGTTATGTTCTGGCTCGTTGGCCGTTGGCAACGATTATACGAGGAGATTGGGGCAATTACGCGcccg                     >  2:1467124/1‑149 (MQ=21)
                                                                                                                                        tGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCaa               >  2:126799/1‑150 (MQ=255)
                                                                                                                                        ccgttgCCTGATGCGACGCTGTGCGTCTTATCAGGCATCGGCTGCCGGATGCGGCGTGAGCGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTAGGCTTTGCGATTGGGGCAATTACGCGCCCTCGTCaa               >  2:116125/6‑150 (MQ=25)
                                                                                                                                          tAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAAt             >  2:657347/1‑150 (MQ=255)
                                                                                                                                           ttgCCTGATGCGACGCTGTGCGTCTTATCAGGCAACGGCTGCCGGATGCGGCGTGACCGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATc            >  1:1494236/3‑150 (MQ=37)
                                                                                                                                           aGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATc            >  2:1237412/1‑150 (MQ=255)
                                                                                                                                            ggCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCCACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCa           >  2:71145/1‑150 (MQ=255)
                                                                                                                                                tGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTgc       >  1:401564/1‑150 (MQ=255)
                                                                                                                                                 gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTgcg      <  1:398522/150‑1 (MQ=255)
                                                                                                                                                 aaTAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTgcg      >  2:1456275/2‑150 (MQ=255)
                                                                                                                                                   tAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTTTCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCt    >  1:824009/1‑150 (MQ=255)
                                                                                                                                                   tAAGACGCACAGCGTCGCATCAGGAAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTTGCTCGTTGGACTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAACTCATTGCGCt    >  2:1755274/1‑150 (MQ=255)
                                                                                                                                                     aGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCttt  <  1:1699187/150‑1 (MQ=255)
                                                                                                                                                     aGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCAGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCttt  >  1:1826083/1‑150 (MQ=255)
                                                                                                                                                     aGACGCACAGCGTCGCATAAGGCAACGGCTGCCGGATGCGCCGTGAACGAACTATCCGACCTAGGGGTATGTTCTGGCTCGTTGGCCTTTGGCAACGATGAGCATATGAGATTGGGGAAATTACGCTCCCTCGTCAACTCATTGAGCatt  >  2:330836/1‑150 (MQ=25)
                                                                                                                                                     |                                                                                                                                                     
GGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTT  >  NC_000913/3392171‑3392469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: