Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,397,967 C→T 5.6% V138I (GTC→ATC) ‡ yhdE ← dTTP/UTP pyrophosphatase; m(5)UTP/m(5)CTP/pseudo‑UTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,397,9670CT 5.6% 402.3 / 7.9 125V138I (GTC→ATC) ‡yhdEdTTP/UTP pyrophosphatase; m(5)UTP/m(5)CTP/pseudo‑UTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (56/62);  new base T (2/5);  total (58/67)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-01

Only 100 of 125 total aligned reads displayed.

TTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCA  >  NC_000913/3397828‑3398115
                                                                                                                                           |                                                                                                                                                    
ttACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCcgcg                                                                                                                                            <  2:444339/150‑1 (MQ=255)
  aCTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGAt                                                                                                                                          <  2:36721/150‑1 (MQ=255)
  aCTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGAt                                                                                                                                          <  2:1049554/150‑1 (MQ=255)
  aCTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGAt                                                                                                                                          <  1:427873/150‑1 (MQ=255)
  aCTAAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGAt                                                                                                                                          <  2:1690833/150‑1 (MQ=255)
   cTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATg                                                                                                                                         <  2:1791859/150‑1 (MQ=255)
   cTTAATAATTCATACGTTGCAACCAGCGGTAAGCCGACTACGGAGGGATAGCTTCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTGCATCGCTGGCGACATATCCCGCGATg                                                                                                                                         <  1:74126/150‑1 (MQ=255)
     taataaTTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGCCATAGCCCGCGATGTc                                                                                                                                       >  1:1272720/1‑150 (MQ=255)
     taataaTTCATACGTTTCAACCACCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTc                                                                                                                                       <  2:984762/150‑1 (MQ=255)
      aataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATCCCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCt                                                                                                                                      >  1:867921/1‑150 (MQ=255)
      aataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCt                                                                                                                                      <  2:20891/150‑1 (MQ=255)
      aataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCCAACGGTTCATCGCGGGCTATGTCGCCAGCGATgaac                                                                                                                                      <  2:136882/150‑4 (MQ=255)
      aataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCACTGCATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGAGATGTCt                                                                                                                                      >  2:1064958/1‑150 (MQ=255)
      aataaaTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCAACCCAGCACCTGAAAACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCt                                                                                                                                      >  2:1418565/1‑150 (MQ=255)
       ataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCtt                                                                                                                                     <  1:130481/150‑1 (MQ=255)
       ataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGAGTGATAGGTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCtt                                                                                                                                     <  2:1358270/150‑1 (MQ=255)
        taatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTc                                                                                                                                    <  1:75932/150‑1 (MQ=255)
          atTCATACGTTTCAACCATCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGt                                                                                                                                  >  2:1376521/1‑150 (MQ=255)
          atTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGAATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGATgaaccgtt                                                                                                                                  <  1:194808/150‑8 (MQ=255)
           ttCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTc                                                                                                                                 <  1:600884/150‑1 (MQ=255)
           ttCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTc                                                                                                                                 <  1:436928/150‑1 (MQ=255)
             cATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCGCCTGAATACCGTATGCACCTGCTTTATCTAACGCTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTg                                                                                                                               <  2:784189/150‑1 (MQ=255)
               tACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGtt                                                                                                                             >  1:57017/1‑150 (MQ=255)
               tACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGtt                                                                                                                             <  1:1658336/150‑1 (MQ=255)
                 cGTTTCACCCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCCCCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTaa                                                                                                                           >  1:1421456/1‑150 (MQ=255)
                 cGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTaa                                                                                                                           >  2:801552/1‑150 (MQ=255)
                 cGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTGTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCGGTTaa                                                                                                                           >  1:226724/1‑150 (MQ=255)
                  gTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTTGCGACATAGCCCGCGATGTCTTCGTCTGTTAAc                                                                                                                          >  2:345013/1‑150 (MQ=255)
                  gTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAAc                                                                                                                          >  2:1305925/1‑150 (MQ=255)
                      cAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGAACCTGCTTTATCTAACGGTTCATCGCTGGCGCCATAGCCCGCGATGTCTTCGTCTGTTAACGtta                                                                                                                      >  1:1154471/1‑149 (MQ=255)
                       aaCCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCt                                                                                                                     <  1:314687/150‑1 (MQ=255)
                           aGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTg                                                                                                                               <  2:220411/136‑1 (MQ=255)
                           aGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGaaa                                                                                                                 >  2:1180626/1‑150 (MQ=255)
                              ggTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGATCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTc                                                                                                              <  2:1583961/150‑1 (MQ=255)
                                   gCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATc                                                                                                         >  2:1025339/1‑150 (MQ=255)
                                    ccGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCg                                                                                                        >  2:583649/1‑150 (MQ=255)
                                     cGACTACGGCGTGATTTCTGCCATTTATCTTCCTGACAATACAGCCACCCAGCCCCTGTATTCCGTATGCACCTGCTTTTTCTATCGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCgg                                                                                                       <  1:252794/150‑1 (MQ=255)
                                         tACGGCGTGATACCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCAGATGAATACCTTATGCACCTGCTTTATCTAACGGTTCTTCGCTGGCGACATATCCCTCGATGTCATCGTCTGTTAACGTTCTGAATGTCACATCGGGGac                                                                                                   <  1:82862/150‑1 (MQ=255)
                                            ggCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGaccac                                                                                                >  1:547067/1‑150 (MQ=255)
                                               gTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAg                                                                                             <  1:1307311/150‑1 (MQ=255)
                                                      cTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCg                                                                                      <  1:1435820/150‑1 (MQ=255)
                                                      cTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCg                                                                                      >  1:1808616/1‑150 (MQ=255)
                                                          cATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGCCAACCAGGCAATCGAGaa                                                                                  >  1:1635138/1‑150 (MQ=255)
                                                          cATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGaa                                                                                  >  1:574312/1‑150 (MQ=255)
                                                              tATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGCCATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGCCCACCAGGCATTCGAGAAtgtg                                                                              >  1:8694/1‑150 (MQ=255)
                                                                 cTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgctg                                                                           <  1:665758/150‑1 (MQ=255)
                                                                 cTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgctg                                                                           >  1:1622794/1‑150 (MQ=255)
                                                                   tCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgctggc                                                                         <  1:746036/150‑1 (MQ=255)
                                                                   tCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgctggc                                                                         <  2:46859/150‑1 (MQ=255)
                                                                      tGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGt                                                                      >  1:1566262/1‑150 (MQ=255)
                                                                      tGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGt                                                                      >  1:124233/1‑150 (MQ=255)
                                                                      tGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGt                                                                      >  1:509016/1‑150 (MQ=255)
                                                                       gACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGAACACCAGGCAATCGAGAATGTGCTGGCTGTc                                                                     >  1:1242201/1‑150 (MQ=255)
                                                                        aCAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCg                                                                    <  1:100317/150‑1 (MQ=255)
                                                                         cAAAACAGCCACCCAGCACCTTAATACCGTATGCACCTGCTTTATCTAACGGTTCATCTCTGGCGACATAGCCCGCTATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGTTGACCAACAGGCAATCGAGAATGTGCTGGATGTCgg                                                                   <  2:1711095/150‑1 (MQ=255)
                                                                          aaaaCAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGc                                                                  >  2:1262235/1‑150 (MQ=255)
                                                                          aaaaCAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGc                                                                  <  2:442397/150‑1 (MQ=255)
                                                                           aaaCAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTATGAAAGTCCCATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGcc                                                                 >  2:305843/1‑150 (MQ=255)
                                                                            aaCAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCa                                                                >  1:1197357/1‑150 (MQ=255)
                                                                            aaCAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCa                                                                >  1:645776/1‑150 (MQ=255)
                                                                                gCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGc                                                            >  2:1046300/1‑150 (MQ=255)
                                                                                 ccACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTAATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCAACAGGCAATCGAGAATGTGCTGGCAGTCGGCCAACGcc                                                           >  2:524094/1‑150 (MQ=255)
                                                                                   aCCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCAc                                                         >  1:1313193/1‑150 (MQ=255)
                                                                                   aCCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCAc                                                         <  2:466916/150‑1 (MQ=255)
                                                                                   aCCCAGCCCCTGAATACCGTATGCACCTGCTTTAGCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCAc                                                         <  1:114725/150‑1 (MQ=255)
                                                                                        gCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCActgctg                                                    <  2:1284173/150‑1 (MQ=255)
                                                                                         ccccTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGt                                                   >  2:438531/1‑150 (MQ=255)
                                                                                              gAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCAGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGtcatca                                              >  1:150749/1‑150 (MQ=255)
                                                                                                aTACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTTACGTTCTGAAAGTCACATCGGTGACCACCACGCAATCGAGAAAGTGCTGGCTGTCGGCCAACGCCCCTGCTGTCATCAcc                                            >  2:627151/1‑150 (MQ=255)
                                                                                                aTACCGTATGCACCTGCATTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCAcc                                            <  1:659146/150‑1 (MQ=255)
                                                                                                 tACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCt                                           >  2:123961/1‑150 (MQ=255)
                                                                                                     gTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATg                                       <  1:1305925/150‑1 (MQ=255)
                                                                                                     gTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATg                                       <  1:1710284/150‑1 (MQ=255)
                                                                                                      tATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATgg                                      <  1:966264/150‑1 (MQ=255)
                                                                                                         gCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTc                                   <  1:908903/150‑1 (MQ=255)
                                                                                                         gCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTc                                   <  1:801552/150‑1 (MQ=255)
                                                                                                            ccTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTTAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACTACACTTCTGTCATCACCTGATGGGTCTgc                                >  1:77113/1‑149 (MQ=255)
                                                                                                              tGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCTAGAATGTGCTGGATGTCGGCCAACGCCACTGCTATCATCACCTGATGTGTCTGAcc                              <  1:176831/150‑1 (MQ=255)
                                                                                                              tGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGagc                              >  2:912749/1‑148 (MQ=255)
                                                                                                                 tttATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGa                           >  1:407629/1‑150 (MQ=255)
                                                                                                                 tttATATAACGGTTCATCGCTGGCGACATACCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACAACAGTGAACACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCCCCGCCACTGCTGTCATCACGGGAGGGGAGTGCCCCGa                           >  2:299507/1‑150 (MQ=255)
                                                                                                                      cTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCCCCTGCTGGGTCTGACCCGATAAtt                      >  1:1212958/1‑150 (MQ=255)
                                                                                                                      cTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAAtt                      <  1:1046300/150‑1 (MQ=255)
                                                                                                                       tAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACGTCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAAttt                     <  1:199049/150‑1 (MQ=255)
                                                                                                                        aaCGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTg                    <  2:505730/150‑1 (MQ=255)
                                                                                                                         aCGGTTCATCGCTGGCGACATAGCCCTCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGAGCTGTCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgc                   <  1:1064958/150‑1 (MQ=255)
                                                                                                                         aCGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGCCCCGATAATTTgc                   >  1:1167706/1‑150 (MQ=255)
                                                                                                                         aCGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgc                   >  1:412230/1‑150 (MQ=255)
                                                                                                                         aCGGTTCATCGCTGGCGACATAGACCGCGATGTCTTCGTCTGTTAACGTTGAGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgc                   <  1:1418565/150‑1 (MQ=255)
                                                                                                                          cGGCTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACCTCGGTGACCACCAGGCAAGCGAGCATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgcg                  <  1:278298/150‑1 (MQ=255)
                                                                                                                            gTTCATCGCTGGCGACATCGCCCGCGATGTCTTCGTCTGTTAACGTGCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCa                <  1:142207/150‑1 (MQ=255)
                                                                                                                            gTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGCTGGGTCTGACCCGATAATTTGCGCa                >  1:964778/1‑150 (MQ=255)
                                                                                                                            gTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCa                >  2:1571303/1‑150 (MQ=255)
                                                                                                                            gTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCa                <  2:1651400/150‑1 (MQ=255)
                                                                                                                              acATCGCGGGCTATGTCGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAAc              >  1:1465802/2‑150 (MQ=255)
                                                                                                                               cATCGCGGGCTATGTCGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCTGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACa             >  2:679430/1‑150 (MQ=255)
                                                                                                                                aTCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACAt            >  2:683382/1‑150 (MQ=255)
                                                                                                                                    cTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTgc        <  1:1068172/150‑1 (MQ=255)
                                                                                                                                    cTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTgc        <  1:1557631/150‑1 (MQ=255)
                                                                                                                                          aCATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCa  <  2:323300/150‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
TTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCA  >  NC_000913/3397828‑3398115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: