Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,430,942 A→C 5.5% F158L (TTT→TTG)  tsaC ← tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,430,9420AC 5.5% 303.2 / 6.2 101F158L (TTT→TTG) tsaCtRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase
Reads supporting (aligned to +/- strand):  ref base A (51/44);  new base C (4/2);  total (55/46)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 103 total aligned reads displayed.

TGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCC  >  NC_000913/3430796‑3431091
                                                                                                                                                  |                                                                                                                                                     
tGCTGTGGGCTATCGGAATACCAAAAACACCAAAGGTTTCCAGAATGTTACCCCACTCGAAACAGTTCACCCGTCAGAGCATCGCGGAATTCTGAAGGATTTAAACGCCACCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCaaaa                                                                                                                                                    >  2:871428/1‑149 (MQ=255)
      gggCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGGTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTgcg                                                                                                                                              <  1:1188506/150‑1 (MQ=255)
          tATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGaa                                                                                                                                          <  2:632708/150‑1 (MQ=255)
          tATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGaa                                                                                                                                          <  2:1489165/150‑1 (MQ=255)
           aTCGGATTACCAAAACCAGCATATGTTTCCATTATGTTACCCCTGTCGCAACAGGTCACCCGTCCGGGCATCGCGGATTTCTGAAGGATGTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAAc                                                                                                                                         <  1:1482203/150‑1 (MQ=255)
            tctgATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCACAATTTGGcgcggcgt                                                                                                                                        <  2:903847/147‑5 (MQ=255)
            tCGGAGTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGGCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCACAATTTGGcgcggcgt                                                                                                                                        <  1:1323829/150‑5 (MQ=255)
             cGGATTACCAAAAACAGCATAGAGTTCCTTTATGTTACCACAGTCGAAACAGTTCACACGTCAGGGCATCGCGGAGTTCTGAACGATTTAAACGCCCCCCAGTTTCACCAGGCACAACCGGGAACGCCGCGCCCAAATGAGCGCCAACtt                                                                                                                                       >  2:1636328/1‑150 (MQ=255)
              ggATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTc                                                                                                                                      >  2:129595/1‑150 (MQ=255)
                 ttACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGACCTTCGTc                                                                                                                                   >  1:1794805/1‑150 (MQ=255)
                   aCCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTa                                                                                                                                 >  1:1406595/1‑150 (MQ=255)
                    ccAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTAc                                                                                                                                >  2:1321448/1‑150 (MQ=255)
                    ccAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTAc                                                                                                                                >  2:856200/1‑150 (MQ=255)
                    ccAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTAc                                                                                                                                <  2:585097/150‑1 (MQ=255)
                    ccAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTAc                                                                                                                                >  1:1068019/1‑150 (MQ=255)
                    ccAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTAc                                                                                                                                >  1:1643115/1‑150 (MQ=255)
                      aaaaaCAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTg                                                                                                                              >  2:708151/1‑150 (MQ=255)
                      aaaaaCAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAAATTCGTCTACTg                                                                                                                              >  1:1604134/1‑150 (MQ=255)
                            aGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGAc                                                                                                                        <  2:811426/150‑1 (MQ=255)
                            aGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGAc                                                                                                                        >  1:6385/1‑150 (MQ=255)
                             gCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCTCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACa                                                                                                                       >  2:1148853/1‑150 (MQ=255)
                             gCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACa                                                                                                                       <  1:569705/150‑1 (MQ=255)
                             gCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACa                                                                                                                       >  2:1818184/1‑150 (MQ=255)
                                   gTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTgg                                                                                                                 <  1:1723037/150‑1 (MQ=255)
                                   gTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTgg                                                                                                                 <  1:1363201/150‑1 (MQ=255)
                                   gTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCAACAGGCACAACCGGGAACGCCGAGCCAAATTGTGAGCGAAGTTCGTCTACTGTTCGACAAGGTgg                                                                                                                 >  2:184734/1‑150 (MQ=255)
                                    tttCCATTATGTTACCCCTGTCGAACCAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCGCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGc                                                                                                                <  2:1557669/150‑1 (MQ=255)
                                      tCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCaa                                                                                                              <  1:1103378/150‑1 (MQ=255)
                                      tCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCaa                                                                                                              <  2:1212277/150‑1 (MQ=255)
                                       ccATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAAt                                                                                                             <  1:540587/150‑1 (MQ=255)
                                       ccATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAAt                                                                                                             >  1:391314/1‑150 (MQ=255)
                                        cATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATc                                                                                                            <  2:1139576/150‑1 (MQ=255)
                                         aTTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATcc                                                                                                           >  2:571083/1‑150 (MQ=255)
                                         aTTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCACCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATcc                                                                                                           <  2:1341095/150‑1 (MQ=255)
                                         aTTATGTTACCCCTGGCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATcc                                                                                                           <  2:1693544/150‑1 (MQ=255)
                                            tttttACCCCTGGCGAAACAGTTCAACCGTCAGGGCATCGCGGTTTTCTGAAGTATTTAAACGCCCCCCCGGTTCACCAGGCACATCCGGGAACGCCGCGCCAAATTGTGCGCGAAGTTCGTCTACTGTTCGACAAGGTGGCAATCCCCt                                                                                                        <  1:921796/147‑1 (MQ=255)
                                              gTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCa                                                                                                      <  2:1608093/150‑1 (MQ=255)
                                              gTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCa                                                                                                      <  2:190507/150‑1 (MQ=255)
                                                tACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAACCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAg                                                                                                    >  1:1643790/1‑150 (MQ=255)
                                                tACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAg                                                                                                    <  2:48693/150‑1 (MQ=255)
                                                  ccccTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGACCGCCGCGCCAAATTGTGCGCGAACGTCGTCTACTGTTCGACAAGGTGGAAATCCACACAAGtt                                                                                                  >  1:1715524/1‑150 (MQ=255)
                                                   cccTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACATCCGGGAACGCCTCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGTCAAGGTGGCAATCCACTCAAGTTg                                                                                                 >  1:131327/1‑150 (MQ=255)
                                                    ccTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCGCCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTgg                                                                                                >  2:747601/1‑150 (MQ=255)
                                                      tGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCa                                                                                              >  2:1539536/1‑150 (MQ=255)
                                                          gAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCTAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTgg                                                                                          >  2:211290/1‑150 (MQ=255)
                                                                 ttCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAAc                                                                                   >  1:619188/1‑150 (MQ=255)
                                                                 ttCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAAc                                                                                   >  2:1380150/1‑150 (MQ=255)
                                                                 ttCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAAc                                                                                   >  1:340737/1‑150 (MQ=255)
                                                                   cACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCa                                                                                 >  2:1353743/1‑150 (MQ=255)
                                                                   cACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCa                                                                                 >  1:1006881/1‑150 (MQ=255)
                                                                    aCCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAg                                                                                <  2:1399881/150‑1 (MQ=255)
                                                                     cccGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGc                                                                               <  1:708151/150‑1 (MQ=255)
                                                                          cAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGttt                                                                          <  2:1208976/150‑1 (MQ=255)
                                                                            gggCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCCCCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGCACTTCGTCTACTGTTCGCCAAGGTGGCACTCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTAc                                                                        >  1:1011542/1‑150 (MQ=255)
                                                                            gggCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTAc                                                                        <  1:1321448/150‑1 (MQ=255)
                                                                            gggCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTAc                                                                        <  1:360555/150‑1 (MQ=255)
                                                                             ggCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTAcc                                                                       >  1:1120106/1‑150 (MQ=255)
                                                                             ggCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACAAGGCACAACCGGGAACGCCGCGCCAACTTGTGCGCTAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTAcc                                                                       <  1:1150738/150‑1 (MQ=255)
                                                                              gCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCCCAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCa                                                                      >  1:821664/1‑150 (MQ=255)
                                                                                aTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGATCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATa                                                                    <  1:222116/150‑1 (MQ=255)
                                                                                   gcgGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCGATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGc                                                                 >  1:912476/1‑150 (MQ=255)
                                                                                   gcgGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGc                                                                 <  1:1035090/150‑1 (MQ=255)
                                                                                      gATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTAAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGGCTg                                                              >  1:354474/1‑150 (MQ=255)
                                                                                             gAAGGATTTAAACGACCCCCCGTTTCACCCGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTAGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAACCCAGCGGTTTACCATAACCCTGGccctaa                                                       >  1:884616/1‑145 (MQ=255)
                                                                                              aaGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAg                                                      <  1:906491/150‑1 (MQ=255)
                                                                                              aaGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAg                                                      <  1:1421315/150‑1 (MQ=255)
                                                                                                ggATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACCTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCa                                                    >  1:634867/1‑150 (MQ=255)
                                                                                                 gagttAAACGCCCCCCCGTTTCACGAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCaa                                                   <  1:320017/147‑1 (MQ=255)
                                                                                                  aTTTAAGCGCCCCCCCGTTTCACCAGGCCCAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGCCAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAACCCGGCGGTTTACCATACGCCTGGCACAAAGCAac                                                  >  1:549931/1‑150 (MQ=255)
                                                                                                   tttAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAACCCAGCGGTTTACCATAAGCCTGGCACAAAGCAacc                                                 >  1:1332425/1‑150 (MQ=255)
                                                                                                     tAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCCAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAaccac                                               >  1:1789415/1‑150 (MQ=255)
                                                                                                      aaaCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCAAAAAGcacccacc                                              >  1:1553017/1‑150 (MQ=255)
                                                                                                       aaCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAAccacca                                             <  2:1752850/150‑1 (MQ=255)
                                                                                                         cGCCCCCCCGTTTCACCTGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAAc                                           <  2:892279/150‑1 (MQ=255)
                                                                                                             cccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGAt                                       >  2:1150116/1‑150 (MQ=255)
                                                                                                             caaccGTTTCACCAGGCACAACCGGGAACGCCGAGCCAAATTCTGCGCGAACTTCGTCTACTGTTCGACAAGGTGTCAAACCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCATGGCACAAAGCAAACACAAACGGAt                                       >  2:825717/4‑150 (MQ=255)
                                                                                                                    ttCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGt                                <  2:1061967/150‑1 (MQ=255)
                                                                                                                    ttCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGt                                <  2:436021/150‑1 (MQ=255)
                                                                                                                    ttCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGt                                <  2:639281/150‑1 (MQ=255)
                                                                                                                    ttCACCAGGCACAAACGGGAACTCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGt                                <  2:429268/150‑1 (MQ=255)
                                                                                                                     tCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTg                               >  2:1472743/1‑150 (MQ=255)
                                                                                                                       aCCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGAc                             >  2:477327/1‑150 (MQ=255)
                                                                                                                       aCCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGAc                             <  1:717627/150‑1 (MQ=255)
                                                                                                                        ccAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGAcg                            >  1:989240/1‑149 (MQ=255)
                                                                                                                         cAGGCACAACCGGGAATGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTc                           >  2:395989/1‑150 (MQ=255)
                                                                                                                           ggCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAACCCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGt                         >  1:1562241/1‑150 (MQ=255)
                                                                                                                             cacaACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTAc                       <  2:1406595/150‑1 (MQ=255)
                                                                                                                              acaACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACa                      >  2:1547964/1‑150 (MQ=255)
                                                                                                                                     ggAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCg               >  1:10878/1‑150 (MQ=255)
                                                                                                                                        agttcgcgCACAATTTGGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAAt            >  1:870135/5‑150 (MQ=255)
                                                                                                                                         gttcgcgCACAATTTGGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCGTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATc           >  1:625192/4‑150 (MQ=255)
                                                                                                                                         gttcgcgCACAATTTGGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATc           >  2:185942/4‑150 (MQ=255)
                                                                                                                                         cgccgcGCCAATTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGGGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATc           <  2:1148627/150‑1 (MQ=255)
                                                                                                                                         cgccgcGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATc           >  2:896108/1‑150 (MQ=255)
                                                                                                                                         cgccgcGCCAAATACTGCGCGAACTTCGTCTACTGTGCGACAAGGTGGCAATCCGCTCAAGTTGGCACTGGTAGAACCCAGCGGTTTACCATACGCCTTGCACAAAGCAACCACACACGGATGGTCCGTGACTCGTACAGCCAGCGACTc           >  2:42809/1‑150 (MQ=255)
                                                                                                                                          gccgcGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCa          >  2:1668665/1‑150 (MQ=255)
                                                                                                                                           ccgcGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCaa         >  1:1074129/1‑150 (MQ=255)
                                                                                                                                              cgcCAAATTGTGCGCGAACTTCGTCGACTGTTCAACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGc      >  2:438764/1‑150 (MQ=255)
                                                                                                                                               gcCAAATTGTGCGCGAACATCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCTACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCg     <  1:76303/150‑1 (MQ=255)
                                                                                                                                                  aaaTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGcc  <  1:1148853/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCC  >  NC_000913/3430796‑3431091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑