Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,510,359 C→A 5.5% L206F (TTG→TTT yhfX ← putative pyridoxal 5'‑phosphate binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,510,3590CA 5.5% 311.8 / 4.9 103L206F (TTG→TTTyhfXputative pyridoxal 5'‑phosphate binding protein
Reads supporting (aligned to +/- strand):  ref base C (43/54);  new base A (3/3);  total (46/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 104 total aligned reads displayed.

GGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAATC  >  NC_000913/3510213‑3510508
                                                                                                                                                  |                                                                                                                                                     
ggTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCaaa                                                                                                                                                    <  1:1323021/150‑1 (MQ=255)
 gTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCaaaa                                                                                                                                                   <  1:498282/150‑1 (MQ=255)
 gTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGAAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCACTACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCaaaa                                                                                                                                                   >  1:1306984/1‑150 (MQ=255)
    cGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACtt                                                                                                                                                >  1:1408748/1‑150 (MQ=255)
       cATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTc                                                                                                                                             <  1:1536220/150‑1 (MQ=255)
         tGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCg                                                                                                                                           <  2:1427619/150‑1 (MQ=255)
         tGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCg                                                                                                                                           <  2:32103/150‑1 (MQ=255)
            gTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGAcc                                                                                                                                        >  1:680765/1‑149 (MQ=255)
            gTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACa                                                                                                                                        <  2:75035/150‑1 (MQ=255)
            gTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACa                                                                                                                                        <  2:1255085/150‑1 (MQ=255)
             tCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGGTGCTCTAGTGCAATTCCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGTGTGTGAAGATTCGGTGTCGGAAAAGTTTTGCCGACac                                                                                                                                       <  1:237718/150‑2 (MQ=255)
              cacaCCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGc                                                                                                                                      <  2:589594/150‑1 (MQ=255)
               acacCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAgac                                                                                                                                     >  1:937299/1‑148 (MQ=255)
               acacCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATAACAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGGGTGAAGATTCGGTTTCGGCCAAACTTTGCCGACCGcc                                                                                                                                     >  1:1215902/1‑150 (MQ=255)
                cacCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCACTACCTGATGTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCt                                                                                                                                    <  1:328843/150‑1 (MQ=255)
                 acCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTc                                                                                                                                   >  2:405829/1‑150 (MQ=255)
                 acCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGAAAAGTTTTGCCGACaccgaa                                                                                                                                   <  1:1053018/150‑6 (MQ=255)
                 acCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGAAAAGTTTTGCCGACaccgaa                                                                                                                                   <  2:629917/150‑6 (MQ=255)
                 acCGTATTGCGCCAGTAATGGCAGCGAAGGGCAGCTGGTCGCTGAAGGCGCGTGCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTc                                                                                                                                   <  1:358745/150‑1 (MQ=255)
                     tATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATcc                                                                                                                               <  2:817848/150‑1 (MQ=255)
                     tATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATcc                                                                                                                               >  2:1568165/1‑150 (MQ=255)
                      aTTGCGCCAGTAATGGCAGCGAAGTGAAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTTTCGGCAAAACTTTTCCGACAGGCTCATcac                                                                                                                              >  1:260240/1‑148 (MQ=255)
                         gcgcCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCaaa                                                                                                                           >  1:313466/1‑150 (MQ=255)
                          cgcCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAg                                                                                                                          <  2:911167/150‑1 (MQ=255)
                          cgcCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAg                                                                                                                          >  2:1504005/1‑150 (MQ=255)
                            ccAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCa                                                                                                                        >  1:1236670/1‑150 (MQ=255)
                            ccAGTAAGGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTGCAGATGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGTCAGCCTCATCCCAAAGCa                                                                                                                        <  2:911809/150‑1 (MQ=255)
                                tAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCa                                                                                                                    <  1:68873/150‑1 (MQ=255)
                                   tGGCAGCGAAGTGCAGCTGGGCGCTGAAGGCGCGTTCAGTTGCTCAAGTTCTATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAATCTTTTCCGACAGCCTCATCCCAAAGCAGGCAAgg                                                                                                                 <  1:1146523/150‑1 (MQ=255)
                                    ggCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAggg                                                                                                                <  1:405829/150‑1 (MQ=255)
                                      cAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGaa                                                                                                              >  1:400290/1‑150 (MQ=255)
                                       aGCGAAGTGCAGCTGGTCGCTGCAGGCGCGTTCAGTTGCTCAAGTGCTATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCCATGCGTTTCCGACCGCCTCATCCCAGAGCAGGCAAGGGaaa                                                                                                             >  1:1118828/1‑150 (MQ=255)
                                        gCGACGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGGGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAAt                                                                                                            <  2:74775/150‑1 (MQ=255)
                                        gCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGatat                                                                                                            >  1:1785010/1‑150 (MQ=255)
                                          gAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGACCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATgg                                                                                                          <  1:1426760/150‑1 (MQ=255)
                                           aaGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATggg                                                                                                         <  1:1256931/150‑1 (MQ=255)
                                           aaGTGCAGCTGGGCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGGGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATggg                                                                                                         <  2:335761/150‑1 (MQ=255)
                                             gTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCACCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTa                                                                                                       <  1:1262491/150‑1 (MQ=255)
                                                cAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCCGTTTCGCCAAAACTATTAATACATACTCATCCCAAAGCAGGCAAGTGAAATGGGTAAGt                                                                                                    >  2:780776/1‑150 (MQ=255)
                                                    tGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCgg                                                                                                >  1:563223/1‑150 (MQ=255)
                                                     ggTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGc                                                                                               <  1:1816533/150‑1 (MQ=255)
                                                       tCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTa                                                                                             <  1:46389/150‑1 (MQ=255)
                                                            gAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCAGACATCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTATATGc                                                                                        >  2:715724/1‑150 (MQ=255)
                                                             aaGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTGCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTATCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCa                                                                                       <  1:228836/150‑1 (MQ=255)
                                                               ggCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATAACGTTTCTCCAAAAATTTTAAAAAAAAAACATCCCAAAGCAGGCAAGGGAAATGGGAAAGTCCGGCTAAATGCAGc                                                                                     >  2:1077510/1‑150 (MQ=255)
                                                                 cgcgTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGccc                                                                                   <  2:1408748/150‑1 (MQ=255)
                                                                 cgcgTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGccc                                                                                   <  2:1094099/150‑1 (MQ=255)
                                                                   ctttCAGTTGCTCAAGTGCAATAACAGATATCTCCAGTTGATCCCGTGCCTGTATCAGATTGTTAAGATTCGTTGTCGGCAAAACTTTTACGACAGACTCATCCAAAAGCATGAAAGTTAAATGGGTAAGTCCGGCTAAATGCAGCCCTg                                                                                 <  2:1687731/148‑1 (MQ=255)
                                                                    gTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTgg                                                                                >  1:1733878/1‑150 (MQ=255)
                                                                     ttCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGc                                                                               <  1:214046/150‑1 (MQ=255)
                                                                       cAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGGCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAg                                                                             <  1:665090/150‑1 (MQ=255)
                                                                         gTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGAt                                                                           <  1:307578/150‑1 (MQ=255)
                                                                         gTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGAt                                                                           <  1:499612/150‑1 (MQ=255)
                                                                             cTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCCTCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTg                                                                       >  1:68033/1‑150 (MQ=255)
                                                                             cTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGAAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTcgg                                                                       >  1:338369/1‑148 (MQ=255)
                                                                              tCAAGTGGATTTCCAGATTTCGCCAGTTGATCCCGTTGCTGTATGAGCGTGTGAAGATTCGGTGTCGGCAAAACTTATCCGACAGCCGCATCCCAACGCAGGCATGGGAAATGGGTAAGGCCGGCTAAATGCAGCCCTGGCAGATTCTgg                                                                      <  1:130096/150‑1 (MQ=255)
                                                                              tCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTgg                                                                      <  2:313466/150‑1 (MQ=255)
                                                                              tCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTgg                                                                      <  2:710572/150‑1 (MQ=255)
                                                                              gcAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGGGAAGATTCGGGGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTgg                                                                      <  1:381791/149‑1 (MQ=255)
                                                                               cAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGa                                                                     <  2:522515/150‑1 (MQ=255)
                                                                                 aGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCTCCAAAACTTTTCATACAACCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGTAtt                                                                   >  2:1133762/1‑150 (MQ=255)
                                                                                  gTGCAATACCAGATTTCGCCAGTTGATCCCGTTCCTGTATCAGCGTTTGAAGATTCTTTGTATGCAAAACTTTTCATAAAGAATAATAAAAAATCAGGCAATGGAAATGTGTAATTCCTCCTAAATGCATCCCTTGCAGATTCTTGAttt                                                                  >  2:957531/1‑150 (MQ=255)
                                                                                   tggaaTACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTTAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTc                                                                 <  1:1077510/147‑1 (MQ=255)
                                                                                    gCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGTAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCg                                                                >  2:1564962/1‑150 (MQ=255)
                                                                                    gCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAATTCCGGCAAAATGCAGCCCTGGCAGATTCTGGATTTCg                                                                >  1:795626/1‑150 (MQ=255)
                                                                                     cAATACCAGATTTCGCCAGTTGATCCCATGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCgg                                                               <  1:1183342/150‑1 (MQ=255)
                                                                                      aaTACCAGATTTCGCCAGTTGATCCCGTGCCTGTCTCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGc                                                              <  1:1300854/150‑1 (MQ=255)
                                                                                       aTACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCg                                                             >  1:1007499/1‑150 (MQ=255)
                                                                                          ccAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACa                                                          >  1:458595/1‑150 (MQ=255)
                                                                                          ccAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACa                                                          >  1:966475/1‑150 (MQ=255)
                                                                                          ccAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACa                                                          >  1:251757/1‑150 (MQ=255)
                                                                                             gATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAAtc                                                       <  2:499248/150‑1 (MQ=255)
                                                                                                 tCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCg                                                   <  2:1701426/150‑1 (MQ=255)
                                                                                                 tCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCg                                                   <  2:1208198/150‑1 (MQ=255)
                                                                                                  cGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAATTGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCgg                                                  >  1:1197607/1‑150 (MQ=255)
                                                                                                  cGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAACTGCAGCCCTGGCAGCTTCTGGATTTCGGCGACAATCTCCgg                                                  >  1:1580325/1‑150 (MQ=255)
                                                                                                   gCCAGTTTATCCCGTGCCTGTATCAGCGTGTGAAGATTCCGTTTCTGCAAAAATTTTAAAAAAAACTAATAACAAAGAAGGCAAGGGAAATGGGTAAGTCCGCAAAAATGCAGCACTGGCAGATTCTGGAGTTCGGCGCCAATCTCCCGc                                                 >  2:980317/1‑150 (MQ=255)
                                                                                                   gCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGc                                                 >  1:1741625/1‑150 (MQ=255)
                                                                                                   gCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGc                                                 >  1:1158205/1‑150 (MQ=255)
                                                                                                    ccAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACATTTCCGACAGCCTCATCCCAAAGAAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCa                                                >  1:1198367/1‑150 (MQ=255)
                                                                                                        ttGATCCCGTGCCTGTATCAGCGTGTGAAGATTCCGTGTCGCCAAAACTAATCAAACACCCTCATCCAAAAGCAGGCAAGGGAAATGGGTACGTCCTGCTAACTGCAGCCCGGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAacac                                            >  2:523886/1‑150 (MQ=255)
                                                                                                         tGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTAATACATCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACAcc                                           >  2:966937/1‑150 (MQ=255)
                                                                                                           aTCCCGTGCCTGTATCAGCGTGTGAAGATAACGTTTCTCCAAAACTAATAAAAAAAAATAATACCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCtt                                         >  2:926959/1‑150 (MQ=255)
                                                                                                              ccGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAg                                      >  1:38019/1‑150 (MQ=255)
                                                                                                                              gtgtGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTcc                      <  1:412282/150‑1 (MQ=255)
                                                                                                                               tgtgAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGCTTTCGGCGACAATCTCCGGCAACCCCTTGCGGGCAGACCCGCTCTccg                     >  1:822303/1‑149 (MQ=255)
                                                                                                                                gtgAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTg                    <  2:1236670/150‑1 (MQ=255)
                                                                                                                                     taTTCGGTGTCGGCAAAACTTTTCCGACAGCCTAATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCg               <  2:201997/149‑1 (MQ=255)
                                                                                                                                     gATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCg               <  2:400290/150‑1 (MQ=255)
                                                                                                                                         ggctgtCGGAAAAGTTTTGCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATa           >  1:1419476/4‑150 (MQ=255)
                                                                                                                                         cGGTTTCGGCAAAACTTTTCCGACAGCCTCATCACTAAGCAGGCAAGGGAAATGGGTACGTCCGTCTAAATTCAGCCCTGGCAGATGCTGGATTTCGGTGACACTCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATa           <  1:1134670/150‑1 (MQ=255)
                                                                                                                                          ggTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCCGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGTTaa          >  1:1597993/1‑150 (MQ=255)
                                                                                                                                            tgtCGGAAAAGTTTTGCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAg        >  2:334831/1‑150 (MQ=255)
                                                                                                                                            tgtCGGAAAAGTTTTGCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAAAACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAg        >  2:1812334/1‑150 (MQ=255)
                                                                                                                                               cGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGaaa     >  2:1446573/1‑150 (MQ=255)
                                                                                                                                                ggCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGATATCGGTAAGTCCGGCTAAATGAAGCCCTGGCAGAATGTTGATTTCGGCGACAATCTCCGGCATCACCTAGCGGGCAAAACCGCTCTCCGGGCCCGGATAAAGaaat    >  1:90905/1‑149 (MQ=255)
                                                                                                                                                ggCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGaaaa    <  1:1568165/150‑1 (MQ=255)
                                                                                                                                                 gCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAat   <  2:1531435/150‑1 (MQ=255)
                                                                                                                                                 gCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAat   <  2:1306984/150‑1 (MQ=255)
                                                                                                                                                  cAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGCCAAAACCGCTCTCCTGGCCCGGAGAAAGAAAatc  >  2:761737/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAATC  >  NC_000913/3510213‑3510508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: