Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,510,364 C→T 5.8% V205I (GTT→ATT) ‡ yhfX ← putative pyridoxal 5'‑phosphate binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,510,3640CT 5.8% 323.8 / 5.8 104V205I (GTT→ATT) ‡yhfXputative pyridoxal 5'‑phosphate binding protein
Reads supporting (aligned to +/- strand):  ref base C (43/53);  new base T (3/3);  total (48/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

Only 100 of 104 total aligned reads displayed.

CGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAATCATC  >  NC_000913/3510217‑3510511
                                                                                                                                                   |                                                                                                                                                   
cGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACtt                                                                                                                                                   >  1:1408748/1‑150 (MQ=255)
   cATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTc                                                                                                                                                <  1:1536220/150‑1 (MQ=255)
     tGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCg                                                                                                                                              <  2:32103/150‑1 (MQ=255)
     tGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCg                                                                                                                                              <  2:1427619/150‑1 (MQ=255)
        gTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGAcc                                                                                                                                           >  1:680765/1‑149 (MQ=255)
        gTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACa                                                                                                                                           <  2:75035/150‑1 (MQ=255)
        gTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACa                                                                                                                                           <  2:1255085/150‑1 (MQ=255)
         tCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGGTGCTCTAGTGCAATTCCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGTGTGTGAAGATTCGGTGTCGGAAAAGTTTTGCCGACac                                                                                                                                          <  1:237718/150‑2 (MQ=255)
          cacaCCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGc                                                                                                                                         <  2:589594/150‑1 (MQ=255)
           acacCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAgac                                                                                                                                        >  1:937299/1‑148 (MQ=255)
           acacCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATAACAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGGGTGAAGATTCGGTTTCGGCCAAACTTTGCCGACCGcc                                                                                                                                        >  1:1215902/1‑150 (MQ=255)
            cacCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCACTACCTGATGTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCt                                                                                                                                       <  1:328843/150‑1 (MQ=255)
             acCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTc                                                                                                                                      >  2:405829/1‑150 (MQ=255)
             acCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGAAAAGTTTTGCCGACaccgaa                                                                                                                                      <  1:1053018/150‑6 (MQ=255)
             acCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGAAAAGTTTTGCCGACaccgaa                                                                                                                                      <  2:629917/150‑6 (MQ=255)
             acCGTATTGCGCCAGTAATGGCAGCGAAGGGCAGCTGGTCGCTGAAGGCGCGTGCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTc                                                                                                                                      <  1:358745/150‑1 (MQ=255)
                 tATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATcc                                                                                                                                  <  2:817848/150‑1 (MQ=255)
                 tATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATcc                                                                                                                                  >  2:1568165/1‑150 (MQ=255)
                  aTTGCGCCAGTAATGGCAGCGAAGTGAAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTTTCGGCAAAACTTTTCCGACAGGCTCATcac                                                                                                                                 >  1:260240/1‑148 (MQ=255)
                     gcgcCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCaaa                                                                                                                              >  1:313466/1‑150 (MQ=255)
                      cgcCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAg                                                                                                                             >  2:1504005/1‑150 (MQ=255)
                      cgcCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAg                                                                                                                             <  2:911167/150‑1 (MQ=255)
                        ccAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCa                                                                                                                           >  1:1236670/1‑150 (MQ=255)
                        ccAGTAAGGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTGCAGATGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGTCAGCCTCATCCCAAAGCa                                                                                                                           <  2:911809/150‑1 (MQ=255)
                            tAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCa                                                                                                                       <  1:68873/150‑1 (MQ=255)
                               tGGCAGCGAAGTGCAGCTGGGCGCTGAAGGCGCGTTCAGTTGCTCAAGTTCTATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAATCTTTTCCGACAGCCTCATCCCAAAGCAGGCAAgg                                                                                                                    <  1:1146523/150‑1 (MQ=255)
                                ggCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAggg                                                                                                                   <  1:405829/150‑1 (MQ=255)
                                  cAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGaa                                                                                                                 >  1:400290/1‑150 (MQ=255)
                                   aGCGAAGTGCAGCTGGTCGCTGCAGGCGCGTTCAGTTGCTCAAGTGCTATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCCATGCGTTTCCGACCGCCTCATCCCAGAGCAGGCAAGGGaaa                                                                                                                >  1:1118828/1‑150 (MQ=255)
                                    gCGACGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGGGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAAt                                                                                                               <  2:74775/150‑1 (MQ=255)
                                    gCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGatat                                                                                                               >  1:1785010/1‑150 (MQ=255)
                                      gAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGACCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATgg                                                                                                             <  1:1426760/150‑1 (MQ=255)
                                       aaGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATggg                                                                                                            <  1:1256931/150‑1 (MQ=255)
                                       aaGTGCAGCTGGGCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGGGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATggg                                                                                                            <  2:335761/150‑1 (MQ=255)
                                         gTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCACCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTa                                                                                                          <  1:1262491/150‑1 (MQ=255)
                                            cAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCCGTTTCGCCAAAACTATTAATACATACTCATCCCAAAGCAGGCAAGTGAAATGGGTAAGt                                                                                                       >  2:780776/1‑150 (MQ=255)
                                                tGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCgg                                                                                                   >  1:563223/1‑150 (MQ=255)
                                                 ggTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGc                                                                                                  <  1:1816533/150‑1 (MQ=255)
                                                   tCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTa                                                                                                <  1:46389/150‑1 (MQ=255)
                                                        gAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCAGACATCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTATATGc                                                                                           >  2:715724/1‑150 (MQ=255)
                                                         aaGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTGCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTATCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCa                                                                                          <  1:228836/150‑1 (MQ=255)
                                                           ggCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATAACGTTTCTCCAAAAATTTTAAAAAAAAAACATCCCAAAGCAGGCAAGGGAAATGGGAAAGTCCGGCTAAATGCAGc                                                                                        >  2:1077510/1‑150 (MQ=255)
                                                             cgcgTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGccc                                                                                      <  2:1408748/150‑1 (MQ=255)
                                                             cgcgTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGccc                                                                                      <  2:1094099/150‑1 (MQ=255)
                                                               ctttCAGTTGCTCAAGTGCAATAACAGATATCTCCAGTTGATCCCGTGCCTGTATCAGATTGTTAAGATTCGTTGTCGGCAAAACTTTTACGACAGACTCATCCAAAAGCATGAAAGTTAAATGGGTAAGTCCGGCTAAATGCAGCCCTg                                                                                    <  2:1687731/148‑1 (MQ=255)
                                                                gTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTgg                                                                                   >  1:1733878/1‑150 (MQ=255)
                                                                 ttCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGc                                                                                  <  1:214046/150‑1 (MQ=255)
                                                                   cAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGGCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAg                                                                                <  1:665090/150‑1 (MQ=255)
                                                                     gTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGAt                                                                              <  1:499612/150‑1 (MQ=255)
                                                                     gTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGAt                                                                              <  1:307578/150‑1 (MQ=255)
                                                                         cTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGAAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTcgg                                                                          >  1:338369/1‑148 (MQ=255)
                                                                          tCAAGTGGATTTCCAGATTTCGCCAGTTGATCCCGTTGCTGTATGAGCGTGTGAAGATTCGGTGTCGGCAAAACTTATCCGACAGCCGCATCCCAACGCAGGCATGGGAAATGGGTAAGGCCGGCTAAATGCAGCCCTGGCAGATTCTgg                                                                         <  1:130096/150‑1 (MQ=255)
                                                                          tCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCGGATTCTgg                                                                         <  1:678496/150‑1 (MQ=255)
                                                                          tCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTgg                                                                         <  2:313466/150‑1 (MQ=255)
                                                                          tCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTgg                                                                         <  2:710572/150‑1 (MQ=255)
                                                                          gcAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGGGAAGATTCGGGGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTgg                                                                         <  1:381791/149‑1 (MQ=255)
                                                                           cAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGa                                                                        <  2:522515/150‑1 (MQ=255)
                                                                             aGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCTCCAAAACTTTTCATACAACCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGTAtt                                                                      >  2:1133762/1‑150 (MQ=255)
                                                                              gTGCAATACCAGATTTCGCCAGTTGATCCCGTTCCTGTATCAGCGTTTGAAGATTCTTTGTATGCAAAACTTTTCATAAAGAATAATAAAAAATCAGGCAATGGAAATGTGTAATTCCTCCTAAATGCATCCCTTGCAGATTCTTGAttt                                                                     >  2:957531/1‑150 (MQ=255)
                                                                               tggaaTACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTTAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTc                                                                    <  1:1077510/147‑1 (MQ=255)
                                                                                gCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGTAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCg                                                                   >  2:1564962/1‑150 (MQ=255)
                                                                                gCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAATTCCGGCAAAATGCAGCCCTGGCAGATTCTGGATTTCg                                                                   >  1:795626/1‑150 (MQ=255)
                                                                                 cAATACCAGATTTCGCCAGTTGATCCCATGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCgg                                                                  <  1:1183342/150‑1 (MQ=255)
                                                                                  aaTACCAGATTTCGCCAGTTGATCCCGTGCCTGTCTCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGc                                                                 <  1:1300854/150‑1 (MQ=255)
                                                                                   aTACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCg                                                                >  1:1007499/1‑150 (MQ=255)
                                                                                      ccAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACa                                                             >  1:966475/1‑150 (MQ=255)
                                                                                      ccAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACa                                                             >  1:251757/1‑150 (MQ=255)
                                                                                      ccAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACa                                                             >  1:458595/1‑150 (MQ=255)
                                                                                         gATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAAtc                                                          <  2:499248/150‑1 (MQ=255)
                                                                                             tCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCg                                                      <  2:1208198/150‑1 (MQ=255)
                                                                                             tCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCg                                                      <  2:1701426/150‑1 (MQ=255)
                                                                                              cGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAATTGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCgg                                                     >  1:1197607/1‑150 (MQ=255)
                                                                                              cGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAACTGCAGCCCTGGCAGCTTCTGGATTTCGGCGACAATCTCCgg                                                     >  1:1580325/1‑150 (MQ=255)
                                                                                               gCCAGTTTATCCCGTGCCTGTATCAGCGTGTGAAGATTCCGTTTCTGCAAAAATTTTAAAAAAAACTAATAACAAAGAAGGCAAGGGAAATGGGTAAGTCCGCAAAAATGCAGCACTGGCAGATTCTGGAGTTCGGCGCCAATCTCCCGc                                                    >  2:980317/1‑150 (MQ=255)
                                                                                               gCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGc                                                    >  1:1741625/1‑150 (MQ=255)
                                                                                               gCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGc                                                    >  1:1158205/1‑150 (MQ=255)
                                                                                                ccAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACATTTCCGACAGCCTCATCCCAAAGAAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCa                                                   >  1:1198367/1‑150 (MQ=255)
                                                                                                    ttGATCCCGTGCCTGTATCAGCGTGTGAAGATTCCGTGTCGCCAAAACTAATCAAACACCCTCATCCAAAAGCAGGCAAGGGAAATGGGTACGTCCTGCTAACTGCAGCCCGGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAacac                                               >  2:523886/1‑150 (MQ=255)
                                                                                                     tGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTAATACATCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACAcc                                              >  2:966937/1‑150 (MQ=255)
                                                                                                       aTCCCGTGCCTGTATCAGCGTGTGAAGATAACGTTTCTCCAAAACTAATAAAAAAAAATAATACCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCtt                                            >  2:926959/1‑150 (MQ=255)
                                                                                                          ccGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAg                                         >  1:38019/1‑150 (MQ=255)
                                                                                                                          gtgtGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTcc                         <  1:412282/150‑1 (MQ=255)
                                                                                                                           tgtgAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGCTTTCGGCGACAATCTCCGGCAACCCCTTGCGGGCAGACCCGCTCTccg                        >  1:822303/1‑149 (MQ=255)
                                                                                                                            gtgAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTg                       <  2:1236670/150‑1 (MQ=255)
                                                                                                                                 taTTCGGTGTCGGCAAAACTTTTCCGACAGCCTAATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCg                  <  2:201997/149‑1 (MQ=255)
                                                                                                                                 gATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCg                  <  2:400290/150‑1 (MQ=255)
                                                                                                                                     ggctgtCGGAAAAGTTTTGCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATa              >  1:1419476/4‑150 (MQ=255)
                                                                                                                                     cGGTTTCGGCAAAACTTTTCCGACAGCCTCATCACTAAGCAGGCAAGGGAAATGGGTACGTCCGTCTAAATTCAGCCCTGGCAGATGCTGGATTTCGGTGACACTCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATa              <  1:1134670/150‑1 (MQ=255)
                                                                                                                                      ggTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCCGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGTTaa             >  1:1597993/1‑150 (MQ=255)
                                                                                                                                        tgtCGGAAAAGTTTTGCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAg           >  2:334831/1‑150 (MQ=255)
                                                                                                                                        tgtCGGAAAAGTTTTGCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAAAACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAg           >  2:1812334/1‑150 (MQ=255)
                                                                                                                                           cGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGaaa        >  2:1446573/1‑150 (MQ=255)
                                                                                                                                            ggCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGATATCGGTAAGTCCGGCTAAATGAAGCCCTGGCAGAATGTTGATTTCGGCGACAATCTCCGGCATCACCTAGCGGGCAAAACCGCTCTCCGGGCCCGGATAAAGaaat       >  1:90905/1‑149 (MQ=255)
                                                                                                                                            ggCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGaaaa       <  1:1568165/150‑1 (MQ=255)
                                                                                                                                             gCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAat      <  2:1531435/150‑1 (MQ=255)
                                                                                                                                             gCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAat      <  2:1306984/150‑1 (MQ=255)
                                                                                                                                              cAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGCCAAAACCGCTCTCCTGGCCCGGAGAAAGAAAatc     >  2:761737/1‑150 (MQ=255)
                                                                                                                                               aaaaCTTTTCCGACAGCCTCATACCAAAGCAGTCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCTACAATCTCCGGCAACACCTTGAGGTCAAAACCGCTCTCCTGGCCCGGATAAAGAAAatca    <  1:1504005/150‑1 (MQ=255)
                                                                                                                                                aaaCTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAatcat   >  1:1284336/1‑150 (MQ=255)
                                                                                                                                                 aaCTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAatcatc  >  1:919839/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAATCATC  >  NC_000913/3510217‑3510511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: