Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,528,479 T→G 6.6% Y671D (TAC→GAC) ‡ yrfF → putative RcsCDB‑response attenuator, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,528,4790TG 6.6% 366.4 / 13.7 122Y671D (TAC→GAC) ‡yrfFputative RcsCDB‑response attenuator, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (61/53);  new base G (4/4);  total (65/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.31e-01

Only 100 of 125 total aligned reads displayed.

CGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAGGTTATG  >  NC_000913/3528330‑3528626
                                                                                                                                                     |                                                                                                                                                   
cGTTTAACGCCGAGGGTATCGTCACAAAATTCTACACTGACGCCTATGGTTCGCAGCATCTTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCGACTGACGATGCTGGGTAGCGCCAttt                                                                                                                                                     <  1:72607/150‑1 (MQ=255)
cGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCAttt                                                                                                                                                     >  2:1296711/1‑150 (MQ=255)
     aaCGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGTCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAAt                                                                                                                                                >  2:837255/1‑150 (MQ=255)
         ccGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCg                                                                                                                                            <  1:607420/150‑1 (MQ=255)
          cGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGt                                                                                                                                           <  2:1381874/150‑1 (MQ=255)
           gAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTa                                                                                                                                          >  2:1037365/1‑150 (MQ=255)
           gAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTGTAAATGGCgct                                                                                                                                          <  2:894262/150‑3 (MQ=255)
           gAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTGTAAATGGCgct                                                                                                                                          <  1:863676/150‑3 (MQ=255)
            aGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTAc                                                                                                                                         >  1:907444/1‑150 (MQ=255)
            aGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTAc                                                                                                                                         <  2:950347/150‑1 (MQ=255)
             gggTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTGTAAATGGCgctac                                                                                                                                        <  1:724508/150‑5 (MQ=255)
              ggTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAg                                                                                                                                       <  2:1601447/150‑1 (MQ=255)
               gTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAgg                                                                                                                                      >  2:373299/1‑150 (MQ=255)
                 aTCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGcc                                                                                                                                    <  2:1068112/150‑1 (MQ=255)
                  tCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCt                                                                                                                                   >  1:540064/1‑150 (MQ=255)
                   cGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTg                                                                                                                                  >  1:1787968/1‑150 (MQ=255)
                      cacaAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGcg                                                                                                                               >  2:798647/1‑150 (MQ=255)
                      cacaAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGcg                                                                                                                               <  1:556137/150‑1 (MQ=255)
                      cacaAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCCTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCGACTGACGATGCTGGGTAGCGCCAGTTACAATGGCGTACAGGCCTGGcg                                                                                                                               >  2:1140275/1‑150 (MQ=255)
                       acaAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGcgt                                                                                                                              <  1:54622/150‑1 (MQ=255)
                         aaaaaTCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGcgtcg                                                                                                                            >  2:377525/1‑150 (MQ=255)
                         aaaaaTCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGcgtcg                                                                                                                            <  2:1752391/150‑1 (MQ=255)
                         aaaaaTCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGcgtcg                                                                                                                            <  1:272548/150‑1 (MQ=255)
                            aaTCTTCACTGACGCCACTGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGGTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTa                                                                                                                         <  2:1649942/150‑1 (MQ=255)
                            aaTCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTa                                                                                                                         <  1:1165228/150‑1 (MQ=255)
                             aTCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTAc                                                                                                                        <  1:1467120/150‑1 (MQ=255)
                               cTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCa                                                                                                                      >  2:1419642/1‑150 (MQ=255)
                                    cTGCCGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGAGCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTAGTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTc                                                                                                                 <  2:14181/150‑1 (MQ=255)
                                    cTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTc                                                                                                                 <  1:1715991/150‑1 (MQ=255)
                                         gCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGCCGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGc                                                                                                            >  1:247765/1‑150 (MQ=255)
                                         gCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGc                                                                                                            <  2:455813/150‑1 (MQ=255)
                                             aTGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTc                                                                                                        >  2:786185/1‑150 (MQ=255)
                                             aTGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGATGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTc                                                                                                        >  2:38888/1‑150 (MQ=255)
                                                gTACGCAGCATATTGGCCTTCATCCGATCCCGGCTCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCa                                                                                                     <  1:75236/150‑1 (MQ=255)
                                                gTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCa                                                                                                     <  1:1317602/150‑1 (MQ=255)
                                                 tACGCAGCATATTGTCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCGATCTCAGCACCACATTGCTGCTACTTACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCat                                                                                                    <  1:873598/150‑1 (MQ=255)
                                                  aCGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCatg                                                                                                   >  1:1175009/1‑150 (MQ=255)
                                                  aCGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCatg                                                                                                   <  1:1036850/150‑1 (MQ=255)
                                                  aCGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCatg                                                                                                   >  2:1087902/1‑150 (MQ=255)
                                                    gcagcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCatgat                                                                                                 >  1:124087/1‑150 (MQ=255)
                                                    gcagcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCATGGCGTCGTTACCAGCGTCATCGCACTCGCatgat                                                                                                 >  2:836448/1‑150 (MQ=255)
                                                     cagcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGCCGTCGTTACCAGAGTCATCGCACTCGCatgatg                                                                                                >  2:248227/1‑150 (MQ=255)
                                                      agcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATgg                                                                                               <  2:529725/150‑1 (MQ=255)
                                                      agcaTATTGCCCTTCATCCGATCCCGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGga                                                                                              <  1:1821218/150‑1 (MQ=255)
                                                       gcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGga                                                                                              <  1:1620721/150‑1 (MQ=255)
                                                       gcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGga                                                                                              <  1:805343/150‑1 (MQ=255)
                                                         atatTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGgaga                                                                                            >  2:916824/1‑150 (MQ=255)
                                                          tatTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGcc                                                                                                                                    <  1:1298093/109‑1 (MQ=255)
                                                          tatTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGcc                                                                                                                                    >  2:1298093/1‑109 (MQ=255)
                                                             tGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCa                                                                                        >  2:1726394/1‑150 (MQ=255)
                                                              ggCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAg                                                                                       >  1:667570/1‑150 (MQ=255)
                                                              ggCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAg                                                                                       >  2:362667/1‑150 (MQ=255)
                                                               gCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAgg                                                                                      >  2:377104/1‑150 (MQ=255)
                                                               gCCTTCATCCGATCCCGGATCGTTCCGGCCTGGGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAgg                                                                                      <  1:1260959/150‑1 (MQ=255)
                                                                       ccGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGCTTCAGGCCtattat                                                                              >  2:1288060/1‑150 (MQ=255)
                                                                       ccGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCtattat                                                                              >  1:788943/1‑150 (MQ=255)
                                                                        cGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCGTCGCACTCGCATGATGGAGATTCAGGCCTATTATg                                                                             >  1:1312446/1‑150 (MQ=255)
                                                                        cGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATg                                                                             >  2:221258/1‑150 (MQ=255)
                                                                        cGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATg                                                                             >  1:1743697/1‑150 (MQ=255)
                                                                        cGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATg                                                                             >  1:30899/1‑150 (MQ=255)
                                                                           tCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGGTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGGCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGaaa                                                                          <  2:553230/150‑1 (MQ=255)
                                                                             ccGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGc                                                                        <  2:1207617/150‑1 (MQ=255)
                                                                              cGGATCGTTCCGGCCTGTGGCGCTATCTCAGCCCCACATTGCTGCTACTGACGATGCTGGGTAGCGCCAGTTACAATGGCGTACAGGCCTGGCGTCGCTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAACGCt                                                                       >  2:1622152/1‑150 (MQ=255)
                                                                                 aTCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGcc                                                                    <  1:1087902/150‑1 (MQ=255)
                                                                                   cGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTg                                                                  <  2:1725412/150‑1 (MQ=255)
                                                                                    gTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTTCCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGa                                                                 >  2:1435086/1‑150 (MQ=255)
                                                                                       ccGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAAcc                                                              >  2:1298174/1‑150 (MQ=255)
                                                                                       ccGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAAcc                                                              <  1:430192/150‑1 (MQ=255)
                                                                                        cGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAAccc                                                             >  2:312904/1‑150 (MQ=255)
                                                                                           ccTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCa                                                          >  2:85867/1‑150 (MQ=255)
                                                                                             tgtgGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAAc                                                        >  2:1561278/1‑150 (MQ=255)
                                                                                              gtgGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACt                                                       <  1:1037365/150‑1 (MQ=255)
                                                                                                     tATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTCGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCAcc                                                <  2:115320/150‑1 (MQ=255)
                                                                                                     tATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCAcc                                                <  2:1230612/150‑1 (MQ=255)
                                                                                                         tcAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTTCCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTTTGTAAGCTGCCTGAACCCGCAACTGATCACCCCtt                                            >  1:987934/1‑150 (MQ=255)
                                                                                                          cAGCACCACATTGCTGCTACTGACGAAGCTGGGTAGCGCCATTTACAATGGCGGACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGAATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTc                                           <  2:221831/150‑1 (MQ=255)
                                                                                                           aGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCa                                          >  2:1062548/1‑150 (MQ=255)
                                                                                                           aGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCa                                          <  1:402558/150‑1 (MQ=255)
                                                                                                           aGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCa                                          <  1:1688407/150‑1 (MQ=255)
                                                                                                                cacaTTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAg                                     <  2:481359/150‑1 (MQ=255)
                                                                                                                  caTTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGcc                                   <  2:1787968/150‑1 (MQ=255)
                                                                                                                       ctgctACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATc                              <  1:1496118/150‑1 (MQ=255)
                                                                                                                        tgctACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCg                             <  2:605732/150‑1 (MQ=255)
                                                                                                                           tACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAAt                          >  1:1638070/1‑150 (MQ=255)
                                                                                                                            aCTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGCACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATa                         >  1:474469/1‑150 (MQ=255)
                                                                                                                            aCTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATa                         <  1:618238/150‑1 (MQ=255)
                                                                                                                               gACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGCTCCCCCCTTCAGAAAGCCTTATCGAATAaca                      >  1:1516797/1‑150 (MQ=255)
                                                                                                                               gACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAaca                      >  1:193709/1‑150 (MQ=255)
                                                                                                                                 cGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCTGAAAGCCTTATCGAATAACACg                    >  2:461701/1‑150 (MQ=255)
                                                                                                                                        gggTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTgcgc             >  2:459422/1‑150 (MQ=255)
                                                                                                                                        gggTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTgcgc             <  1:786185/150‑1 (MQ=255)
                                                                                                                                        gggTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTgcgc             >  1:498726/1‑150 (MQ=255)
                                                                                                                                        cctgtacgcCATTGTAAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTgcgc             >  1:1501972/7‑150 (MQ=255)
                                                                                                                                        cctgtacgcCATTGTAAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTATgcgc             >  1:810053/7‑150 (MQ=255)
                                                                                                                                           gtacgcCATTGTAAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGc          >  1:641671/4‑150 (MQ=255)
                                                                                                                                            aGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCACCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGTACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCa         <  1:1296711/150‑1 (MQ=255)
                                                                                                                                             acgcCATTGTAAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAg        >  1:592262/2‑150 (MQ=255)
                                                                                                                                              cgcCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCCCCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAgg       >  1:1684535/1‑150 (MQ=255)
                                                                                                                                                  aTTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAGGTTAt   >  1:1018980/1‑150 (MQ=255)
                                                                                                                                                   tttACTATGGCGTACAGGCCTGGCGTCGTTAACAGCTTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTCCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAGGTTATg  <  1:38888/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAGGTTATG  >  NC_000913/3528330‑3528626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: