Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,528,481 C→A 6.3% Y671* (TAC→TAA) ‡ yrfF → putative RcsCDB‑response attenuator, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,528,4810CA 6.3% 371.4 / 9.8 122Y671* (TAC→TAA) ‡yrfFputative RcsCDB‑response attenuator, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (60/53);  new base A (4/4);  total (65/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 123 total aligned reads displayed.

AACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAGGTTATGCTACCC  >  NC_000913/3528335‑3528632
                                                                                                                                                  |                                                                                                                                                       
aaCGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGTCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAAt                                                                                                                                                      >  2:837255/1‑150 (MQ=255)
 aCGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATg                                                                                                                                                     <  2:111938/150‑1 (MQ=255)
    ccGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCg                                                                                                                                                  <  1:607420/150‑1 (MQ=255)
    ccGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCg                                                                                                                                                  <  1:1568027/150‑1 (MQ=255)
     cGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGt                                                                                                                                                 <  2:1381874/150‑1 (MQ=255)
     cGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGt                                                                                                                                                 >  2:1010222/1‑150 (MQ=255)
      gtgggTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCATCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTGTAAATGGCgct                                                                                                                                                <  2:123117/148‑3 (MQ=255)
      gAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTa                                                                                                                                                >  2:1037365/1‑150 (MQ=255)
      gAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTGTAAATGGCgct                                                                                                                                                <  2:894262/150‑3 (MQ=255)
       aGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTAc                                                                                                                                               <  2:950347/150‑1 (MQ=255)
       aGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTAc                                                                                                                                               >  1:907444/1‑150 (MQ=255)
        gggTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTGTAAATGGCgctac                                                                                                                                              <  1:724508/150‑5 (MQ=255)
         ggTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAg                                                                                                                                             <  2:1601447/150‑1 (MQ=255)
          gTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAgg                                                                                                                                            >  2:373299/1‑150 (MQ=255)
            aTCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGcc                                                                                                                                          <  2:1068112/150‑1 (MQ=255)
             tCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCt                                                                                                                                         >  1:540064/1‑150 (MQ=255)
             tCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCt                                                                                                                                         >  1:560870/1‑150 (MQ=255)
              cGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTg                                                                                                                                        >  1:1787968/1‑150 (MQ=255)
                 cacaAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGcg                                                                                                                                     >  2:798647/1‑150 (MQ=255)
                  acaAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGcgt                                                                                                                                    <  1:54622/150‑1 (MQ=255)
                   caAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGcgtc                                                                                                                                   >  1:1127020/1‑150 (MQ=255)
                   caAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGcgtc                                                                                                                                   >  1:19027/1‑150 (MQ=255)
                       aaTCTTCACTGACGCCACTGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGGTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTa                                                                                                                               <  2:1649942/150‑1 (MQ=255)
                        aTCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTAc                                                                                                                              <  1:1467120/150‑1 (MQ=255)
                               cTGCCGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGAGCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTAGTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTc                                                                                                                       <  2:14181/150‑1 (MQ=255)
                               cTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTc                                                                                                                       <  1:1715991/150‑1 (MQ=255)
                                    gCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGCCGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGc                                                                                                                  >  1:247765/1‑150 (MQ=255)
                                    gCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGc                                                                                                                  <  2:455813/150‑1 (MQ=255)
                                        aTGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTc                                                                                                              >  2:786185/1‑150 (MQ=255)
                                        aTGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGATGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTc                                                                                                              >  2:38888/1‑150 (MQ=255)
                                         tGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCg                                                                                                             <  2:353674/150‑1 (MQ=255)
                                           gTACGCAGCATATTGGCCTTCATCCGATCCCGGCTCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCa                                                                                                           <  1:75236/150‑1 (MQ=255)
                                           gTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCa                                                                                                           <  1:1420109/150‑1 (MQ=255)
                                           gTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCa                                                                                                           <  1:1317602/150‑1 (MQ=255)
                                            tACGCAGCATATTGTCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCGATCTCAGCACCACATTGCTGCTACTTACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCat                                                                                                          <  1:873598/150‑1 (MQ=255)
                                             aCGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCatg                                                                                                         >  2:1087902/1‑150 (MQ=255)
                                             aCGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCatg                                                                                                         <  1:1036850/150‑1 (MQ=255)
                                             aCGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCatg                                                                                                         >  1:1175009/1‑150 (MQ=255)
                                               gcagcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCatgat                                                                                                       >  1:124087/1‑150 (MQ=255)
                                               gcagcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCATGGCGTCGTTACCAGCGTCATCGCACTCGCatgat                                                                                                       >  2:836448/1‑150 (MQ=255)
                                                cagcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCatgatg                                                                                                      >  1:481359/1‑150 (MQ=255)
                                                cagcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGCCGTCGTTACCAGAGTCATCGCACTCGCatgatg                                                                                                      >  2:248227/1‑150 (MQ=255)
                                                 agcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATgg                                                                                                     <  2:529725/150‑1 (MQ=255)
                                                 agcaTATTGCCCTTCATCCGATCCCGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGga                                                                                                    <  1:1821218/150‑1 (MQ=255)
                                                  gcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGga                                                                                                    <  1:1620721/150‑1 (MQ=255)
                                                  gcaTATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGga                                                                                                    <  1:805343/150‑1 (MQ=255)
                                                    atatTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGgaga                                                                                                  >  2:916824/1‑150 (MQ=255)
                                                     tatTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGcc                                                                                                                                          <  1:1298093/109‑1 (MQ=255)
                                                     tatTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGcc                                                                                                                                          >  2:1298093/1‑109 (MQ=255)
                                                        tGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCa                                                                                              >  2:1726394/1‑150 (MQ=255)
                                                         ggCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAg                                                                                             >  1:667570/1‑150 (MQ=255)
                                                         ggCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAg                                                                                             >  2:362667/1‑150 (MQ=255)
                                                          gCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAgg                                                                                            >  2:377104/1‑150 (MQ=255)
                                                          gCCTTCATCCGATCCCGGATCGTTCCGGCCTGGGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAgg                                                                                            <  1:1260959/150‑1 (MQ=255)
                                                           ccTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGc                                                                                           >  1:1280502/1‑150 (MQ=255)
                                                               cATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCtat                                                                                       >  2:1688407/1‑150 (MQ=255)
                                                                  ccGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCtattat                                                                                    >  1:788943/1‑150 (MQ=255)
                                                                   cGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCGTCGCACTCGCATGATGGAGATTCAGGCCTATTATg                                                                                   >  1:1312446/1‑150 (MQ=255)
                                                                   cGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATg                                                                                   >  2:221258/1‑150 (MQ=255)
                                                                   cGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATg                                                                                   >  1:30899/1‑150 (MQ=255)
                                                                      tCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGGTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGGCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGaaa                                                                                <  2:553230/150‑1 (MQ=255)
                                                                      tCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGaaa                                                                                <  2:467523/150‑1 (MQ=255)
                                                                        ccGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGc                                                                              <  2:1207617/150‑1 (MQ=255)
                                                                        ccGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGc                                                                              >  1:1704818/1‑150 (MQ=255)
                                                                            aTCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGcc                                                                          <  1:1087902/150‑1 (MQ=255)
                                                                              cGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTg                                                                        <  2:1725412/150‑1 (MQ=255)
                                                                               gTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTTCCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGa                                                                       >  2:1435086/1‑150 (MQ=255)
                                                                                  ccGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAAcc                                                                    >  2:1298174/1‑150 (MQ=255)
                                                                                  ccGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAAcc                                                                    <  1:430192/150‑1 (MQ=255)
                                                                                      ccTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCa                                                                >  2:85867/1‑150 (MQ=255)
                                                                                        tgtgGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAAc                                                              >  2:1561278/1‑150 (MQ=255)
                                                                                         gtgGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACt                                                             <  1:1037365/150‑1 (MQ=255)
                                                                                                tATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTCGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCAcc                                                      <  2:115320/150‑1 (MQ=255)
                                                                                                tATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCAcc                                                      <  2:1230612/150‑1 (MQ=255)
                                                                                                 aTCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCAccc                                                     <  1:765708/150‑1 (MQ=255)
                                                                                                    tcAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTTCCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTTTGTAAGCTGCCTGAACCCGCAACTGATCACCCCtt                                                  >  1:987934/1‑150 (MQ=255)
                                                                                                     cAGCACCACATTGCTGCTACTGACGAAGCTGGGTAGCGCCATTTACAATGGCGGACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGAATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTc                                                 <  2:221831/150‑1 (MQ=255)
                                                                                                      aGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCa                                                <  1:1688407/150‑1 (MQ=255)
                                                                                                      aGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCa                                                <  1:402558/150‑1 (MQ=255)
                                                                                                          ccacATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGaaa                                            >  2:382732/1‑150 (MQ=255)
                                                                                                           cacaTTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAg                                           <  2:481359/150‑1 (MQ=255)
                                                                                                             caTTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGcc                                         <  2:1787968/150‑1 (MQ=255)
                                                                                                                   tgctACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCg                                   <  2:605732/150‑1 (MQ=255)
                                                                                                                       aCTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATa                               <  1:618238/150‑1 (MQ=255)
                                                                                                                       aCTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATa                               >  2:1327340/1‑150 (MQ=255)
                                                                                                                          gACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGCTCCCCCCTTCAGAAAGCCTTATCGAATAaca                            >  1:1516797/1‑150 (MQ=255)
                                                                                                                          gACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAaca                            >  1:193709/1‑150 (MQ=255)
                                                                                                                                   gggTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTgcgc                   >  2:459422/1‑150 (MQ=255)
                                                                                                                                   gggTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTgcgc                   >  1:498726/1‑150 (MQ=255)
                                                                                                                                   cctgtacgcCATTGTAAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTgcgc                   >  1:1501972/7‑150 (MQ=255)
                                                                                                                                   cctgtacgcCATTGTAAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTATgcgc                   >  1:810053/7‑150 (MQ=255)
                                                                                                                                      gtacgcCATTGTAAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGc                >  1:641671/4‑150 (MQ=255)
                                                                                                                                       aGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCa               >  2:1813831/1‑150 (MQ=255)
                                                                                                                                        gcgcCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAg              <  2:1529085/150‑1 (MQ=255)
                                                                                                                                        acgcCATTGTAAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAg              >  1:592262/2‑150 (MQ=255)
                                                                                                                                         cgcCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCCCCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAgg             >  1:1684535/1‑150 (MQ=255)
                                                                                                                                         cgcCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAgg             <  1:837255/150‑1 (MQ=255)
                                                                                                                                             aTTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAGGTTAt         >  1:1018980/1‑150 (MQ=255)
                                                                                                                                              tttACTATGGCGTACAGGCCTGGCGTCGTTAACAGCTTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTCCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAGGTTATg        <  1:38888/150‑1 (MQ=255)
                                                                                                                                                  cAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTAT‑‑TGAAAGCTGCCTGAACCCGCAACTGATCACACCTTAAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAGGTTATGCTAccc  >  2:1114239/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                       
AACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCCTTCATCCGATCCCGGATCGTTCCGGCCTGTGGCGCTATCTCAGCACCACATTGCTGCTACTGACGATGCTGGGTAGCGCCATTTACAATGGCGTACAGGCCTGGCGTCGTTACCAGCGTCATCGCACTCGCATGATGGAGATTCAGGCCTATTATGAAAGCTGCCTGAACCCGCAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAGGTTATGCTACCC  >  NC_000913/3528335‑3528632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: