Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,530,671 A→C 5.1% intergenic (+19/+44) hslO → / ← yhgE heat shock protein Hsp33/DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,530,6710AC 5.1% 443.1 / 10.0 150intergenic (+19/+44)hslO/yhgEheat shock protein Hsp33/DUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (70/72);  new base C (4/4);  total (74/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

Only 100 of 156 total aligned reads displayed.

ATAGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAT  >  NC_000913/3530522‑3530817
                                                                                                                                                     |                                                                                                                                                  
aTAGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCagaga                                                                                                                                                    >  1:1520354/1‑150 (MQ=255)
aTAGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGAAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCagaga                                                                                                                                                    >  1:1703835/1‑150 (MQ=255)
  aGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAgg                                                                                                                                                  >  1:68756/1‑150 (MQ=255)
   gCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAggg                                                                                                                                                 >  1:1239973/1‑150 (MQ=255)
    cATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGc                                                                                                                                                >  2:910347/1‑150 (MQ=255)
    cATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGc                                                                                                                                                <  2:598370/150‑1 (MQ=255)
        cTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTAAATGAGAAGGATATTGCTTAAATCCGCAACAACGCGTCTCAGTCAGATCCGCAAGTTCATTAATGATTATGTCCGACAGACAGATCTCTg                                                                                                                                            >  1:146939/1‑150 (MQ=255)
           gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTAAGTCCGGCAGAGCCCTCTCTGCCg                                                                                                                                         <  1:1145346/150‑1 (MQ=255)
            cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATTTCCGGCAGAGAGGGCTCTGCCgg                                                                                                                                        <  1:1770333/150‑1 (MQ=255)
            cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTg                                                                                                                                            <  2:749291/146‑1 (MQ=255)
            cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCgg                                                                                                                                        >  1:794075/1‑150 (MQ=255)
            cGGAAGATGGCGAAAGTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATGGCGGAAAGCCGCCACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCgg                                                                                                                                        <  1:989803/150‑1 (MQ=255)
            cGGAAGAAGGCGAAATTGACATGCATTGTGATTACTGCCGTCACCACTAGCTGTTCAATGCGATGGATCTAGCTGACATCCGCAACAACGCGTCTCCGGCAGATCCGCAATTTCATTAATGAGTATGTCCGGCAGAGAGTGCTCTGCCgg                                                                                                                                        <  2:1478234/150‑1 (MQ=255)
             ggAAGATGGCGAAATTGCGACGCAGTGAGATTACTGCGGCTACCACTATCTGTTCAATGCGCTGGATTTTGCTGAAATCCGCAACTACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCGGa                                                                                                                                       <  2:297194/150‑1 (MQ=255)
                aGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATAGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGTAttt                                                                                                                                    >  1:888069/1‑150 (MQ=255)
                   tGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAAGGCGATGGATATTGCTGAAATCAGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTATTGAGTATGTCCTGCAGAGAGGGCTCTGCCGGATATTAc                                                                                                                                 >  1:260516/1‑150 (MQ=255)
                   tGGCGAAATTGACATCCATTGTTATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTAc                                                                                                                                 <  1:329697/150‑1 (MQ=255)
                    ggCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACg                                                                                                                                <  1:1076117/150‑1 (MQ=255)
                     gCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGCTCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGa                                                                                                                               >  2:539897/1‑150 (MQ=255)
                     gCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGa                                                                                                                               >  1:1394655/1‑150 (MQ=255)
                     gCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGAGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATTTCCGGCAGAGAGGGCTCTGCCGGATTTTACGa                                                                                                                               >  2:125339/1‑150 (MQ=255)
                        aaaTTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTa                                                                                                                            >  2:792238/1‑150 (MQ=255)
                        aaaTTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTa                                                                                                                            >  2:67166/1‑150 (MQ=255)
                          aTTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAAAGCGATGGATATTGCTGAAATCCGCAACAACGCGGCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAAt                                                                                                                          <  1:1226223/150‑1 (MQ=255)
                           ttGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATa                                                                                                                         <  1:1606969/150‑1 (MQ=255)
                           ttGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATa                                                                                                                         >  2:365358/1‑150 (MQ=255)
                               caacctttGTGATTACGGCGGGAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCACCAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACAcc                                                                                                                     <  1:271188/144‑1 (MQ=255)
                               cATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACt                                                                                                                             >  1:1500161/1‑142 (MQ=255)
                               cATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACAcc                                                                                                                     <  2:898389/150‑1 (MQ=255)
                                   cATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGAt                                                                                                                 <  1:1407369/150‑1 (MQ=255)
                                      tgtgATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGGCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATgcg                                                                                                              <  1:1195670/150‑1 (MQ=255)
                                         gATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCtt                                                                                                           >  1:506335/1‑150 (MQ=255)
                                         gATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCtt                                                                                                           >  1:1422856/1‑150 (MQ=255)
                                           ttACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTg                                                                                                         <  1:87350/150‑1 (MQ=255)
                                           ttACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGAAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTg                                                                                                         <  1:500290/150‑1 (MQ=255)
                                            tACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGt                                                                                                        >  1:362146/1‑150 (MQ=255)
                                             aCTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTc                                                                                                       <  2:133788/150‑1 (MQ=255)
                                             aCTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTc                                                                                                       <  1:590185/150‑1 (MQ=255)
                                             aCTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTc                                                                                                       <  2:752028/150‑1 (MQ=255)
                                                   gTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCAt                                                                                                 >  2:1749219/1‑150 (MQ=255)
                                                    tAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCGGCCGGATTTTACGACTAATACACCTGATGCGCTTTGCCACTCAtt                                                                                                >  2:192016/1‑150 (MQ=255)
                                                     aaCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTc                                                                                               <  2:482139/150‑1 (MQ=255)
                                                     aaCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTc                                                                                               <  1:1610592/150‑1 (MQ=255)
                                                      aCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCTGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCa                                                                                              <  2:871909/150‑1 (MQ=255)
                                                       ccACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAg                                                                                             >  2:565524/1‑150 (MQ=255)
                                                         aCTATCTGTTCAATGCGTTGGATATTGCGTAAATCCGCAACAACGCGTCTCCGGCAGATCCGCATGTTCATTAATGAGTTTTTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTATTACACCTGATGCGCTTTGTCACTCATTCAGAt                                                                                           <  2:246759/150‑1 (MQ=255)
                                                         aCTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGAt                                                                                           <  1:243240/150‑1 (MQ=255)
                                                          cTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGGATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGAtt                                                                                          <  2:1364350/150‑1 (MQ=255)
                                                            aTCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGAAGCGCTTTGTCACTCATTCAGATTca                                                                                        >  1:78462/1‑150 (MQ=255)
                                                               tGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACAt                                                                                     >  1:651328/1‑150 (MQ=255)
                                                                gTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCGGGAGTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCCCAtt                                                                                    >  2:1619931/1‑150 (MQ=255)
                                                                gTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACAtt                                                                                    >  2:284526/1‑150 (MQ=255)
                                                                      tGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGTCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGCTGCGCTTTGTCACTCCTTCAGATTAACATTCAGCCt                                                                              >  2:1208045/1‑150 (MQ=255)
                                                                       gCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCtt                                                                             >  2:1112065/1‑150 (MQ=255)
                                                                        cGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGCCTGATACCCCGGATGCGCTTTGGCACTCATTCAGATTCACATTCAGCCttt                                                                            >  2:112066/1‑150 (MQ=255)
                                                                         gATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCCGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTc                                                                           <  2:37252/150‑1 (MQ=255)
                                                                             gATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCa                                                                       <  2:1537869/150‑1 (MQ=255)
                                                                              tttttgttgAAATCCGCTAGATCGCGTCTCCGGAAGTTCAGAAAGTTAATTATTGATTATGTCCGGCAGAGAGGGCTATGGCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCAt                                                                      <  1:796348/147‑1 (MQ=255)
                                                                               tatTTCTGAAATCATAAACAACGCGTCTCCGGCAGATCCGCTAGTTCATTAATGTGTATGTCCGGCAGAGAGGGGTCTGCCGGATTTTAAGACTTATACAGCTGATGCGCTTTGTCACTCATTCAGTTTCACATTCAGGCTTTCACCATc                                                                     <  2:403117/150‑1 (MQ=255)
                                                                               tatTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATc                                                                     <  2:1406738/150‑1 (MQ=255)
                                                                                 ttGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCa                                                                   <  2:1792831/150‑1 (MQ=255)
                                                                                      gAAATCCGCAACAACGCGTCTCCGGCAGCTCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCGTTCACCATCCACTACa                                                              >  2:147865/1‑150 (MQ=255)
                                                                                         aTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGa                                                           >  1:504980/1‑150 (MQ=255)
                                                                                         aTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGa                                                           >  1:204971/1‑150 (MQ=255)
                                                                                          tCCGCAACAACGCGTCTCCGTCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGAt                                                          <  1:921716/150‑1 (MQ=255)
                                                                                            cGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATcg                                                        >  2:1431506/1‑150 (MQ=255)
                                                                                             gCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATcgc                                                       >  2:836213/1‑150 (MQ=255)
                                                                                                    cgcgTCTCCGGCAGATCCGCAACTTCATTAATGAGTATGTCCGACAGAGAGGGCTCTGCAGGATTTTACGACTAAAACAACTGATGCGCTTTGCCACTCATTCAGATTCACCTTCAGCCTTTAACCATCCACTACAAGATCGCGCCACGc                                                >  1:1788545/1‑150 (MQ=255)
                                                                                                      cgTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCtt                                              >  1:491058/1‑150 (MQ=255)
                                                                                                       gTCTCCGGCAGATCCGCAAGTGCATTAATGAGTATGTCCGGCAGAGAGGACTCTGCCGGATTATACGACTAATACAACTGATGCGCTTTGTCACGCATTCAGATTCACATTCCTGGTTTCCCCATCCACTACAAGATCGCGCCAcccaat                                             >  2:106238/1‑145 (MQ=255)
                                                                                                        tctcCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCGCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTa                                            <  1:173835/150‑1 (MQ=255)
                                                                                                         ctcCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCAACGCTTTAg                                           >  1:982158/1‑150 (MQ=255)
                                                                                                          tcCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAgg                                          <  2:1520354/150‑1 (MQ=255)
                                                                                                           ccGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGc                                         >  2:366046/1‑150 (MQ=255)
                                                                                                            cGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCt                                        <  1:1289763/150‑1 (MQ=255)
                                                                                                                 gATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTc                                   <  2:154979/150‑1 (MQ=255)
                                                                                                                    ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcccc                                <  1:125339/150‑1 (MQ=255)
                                                                                                                    ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACGACAAGATCGCGCCACGCTTTAGGCTTCGTcccc                                >  2:1500198/1‑150 (MQ=255)
                                                                                                                     cGCAAGTTCATTAATGAGTATGTCCTGCAGAGAGGGCTCTGCCGGATTTAACGACTAATACTCCTGATGAGCTTTGTCACTCATACAGAATCACATTCAGCCATTCACCATCCACTACAAGATAGCGCCACGCTTTAGGATTCGTaccag                               >  2:107807/1‑148 (MQ=255)
                                                                                                                     cGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCa                               >  1:297272/1‑150 (MQ=255)
                                                                                                                          gTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGtttt                          <  1:63104/150‑1 (MQ=255)
                                                                                                                            tCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTTATACACCTGATGCGGTTTGTCACTCCTTCAGAGTCACCTTCAGCCTTTCACCATGCACTACAAGATCGCGCCACGCTGTAGGCTTGGTCGCCAGTTTTcc                        >  1:266470/1‑150 (MQ=255)
                                                                                                                            tCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTcc                        >  2:1822165/1‑150 (MQ=255)
                                                                                                                            tCATTAATGAGTATGTCCGGCAGAGAGGGCTATGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTAACATTAAGCCTTTAAACATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTcc                        >  2:1330997/1‑150 (MQ=255)
                                                                                                                             cATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCa                       >  2:1667357/1‑150 (MQ=255)
                                                                                                                                 aaTGAGTATGTCCGGCAGAGAGGGCTCTGCCGGAGTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGGTTTCCATCCt                   >  1:1120809/1‑150 (MQ=255)
                                                                                                                                    gAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCg                <  1:757400/150‑1 (MQ=255)
                                                                                                                                    gAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCg                >  2:67130/1‑150 (MQ=255)
                                                                                                                                    gAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCg                >  2:716887/1‑150 (MQ=255)
                                                                                                                                        aTGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGc            <  2:1367151/150‑1 (MQ=255)
                                                                                                                                         tGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGcg           >  1:784920/1‑149 (MQ=255)
                                                                                                                                          atCCGGCAGAGCCCTCTCTGCCGGATTATACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTGCGCAGCtg          >  1:333942/2‑150 (MQ=255)
                                                                                                                                            ccGGCAGAGCCCTCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg        >  1:1825625/1‑150 (MQ=255)
                                                                                                                                            ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg        <  1:284526/150‑1 (MQ=255)
                                                                                                                                            ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg        <  2:1548847/150‑1 (MQ=255)
                                                                                                                                            ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTAAGCCTTTCACCTTCCACTACAAGATCGCGCCACTCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg        <  2:1133428/150‑1 (MQ=255)
                                                                                                                                              ggCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAg      <  2:41344/150‑1 (MQ=255)
                                                                                                                                              ggCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAg      <  1:1796931/150‑1 (MQ=255)
                                                                                                                                               gCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGt     >  1:1278133/1‑150 (MQ=255)
                                                                                                                                                 agagagGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTaa   >  1:219705/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATAGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAT  >  NC_000913/3530522‑3530817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: