Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,530,672 G→C 5.5% intergenic (+20/+43) hslO → / ← yhgE heat shock protein Hsp33/DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,530,6720GC 5.5% 459.1 / 15.1 146intergenic (+20/+43)hslO/yhgEheat shock protein Hsp33/DUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (67/71);  new base C (4/4);  total (71/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01

Only 100 of 153 total aligned reads displayed.

AGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAT  >  NC_000913/3530524‑3530817
                                                                                                                                                    |                                                                                                                                                 
aGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAgg                                                                                                                                                  >  1:68756/1‑150 (MQ=255)
 gCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAggg                                                                                                                                                 <  2:1715169/150‑1 (MQ=255)
 gCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAggg                                                                                                                                                 >  1:1239973/1‑150 (MQ=255)
  cATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGc                                                                                                                                                >  2:910347/1‑150 (MQ=255)
      cTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTg                                                                                                                                            >  1:1499442/1‑150 (MQ=255)
      cTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTAAATGAGAAGGATATTGCTTAAATCCGCAACAACGCGTCTCAGTCAGATCCGCAAGTTCATTAATGATTATGTCCGACAGACAGATCTCTg                                                                                                                                            >  1:146939/1‑150 (MQ=255)
         gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACTACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCg                                                                                                                                         <  1:341931/150‑1 (MQ=255)
         gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCg                                                                                                                                         <  1:1594419/150‑1 (MQ=255)
         gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTAAGTCCGGCAGAGCCCTCTCTGCCg                                                                                                                                         <  1:1145346/150‑1 (MQ=255)
          cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATTTCCGGCAGAGAGGGCTCTGCCgg                                                                                                                                        <  1:1770333/150‑1 (MQ=255)
          cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTg                                                                                                                                            <  2:749291/146‑1 (MQ=255)
          cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTg                                                                                                                                            >  1:749291/1‑146 (MQ=255)
          cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCgg                                                                                                                                        >  1:794075/1‑150 (MQ=255)
          cGGAAGATGGCGAAAGTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATGGCGGAAAGCCGCCACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCgg                                                                                                                                        <  1:989803/150‑1 (MQ=255)
                 tGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTAc                                                                                                                                 >  2:1571648/1‑150 (MQ=255)
                 tGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTAc                                                                                                                                 >  2:1289763/1‑150 (MQ=255)
                 tGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAAGGCGATGGATATTGCTGAAATCAGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTATTGAGTATGTCCTGCAGAGAGGGCTCTGCCGGATATTAc                                                                                                                                 >  1:260516/1‑150 (MQ=255)
                  ggCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACg                                                                                                                                <  1:1076117/150‑1 (MQ=255)
                   gCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGAGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATTTCCGGCAGAGAGGGCTCTGCCGGATTTTACGa                                                                                                                               >  2:125339/1‑150 (MQ=255)
                      aaaTTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTa                                                                                                                            >  2:792238/1‑150 (MQ=255)
                        aTTTACATGCATTGTGCTTACTGCGGTAACCACTACCTGGTCAATGCGATGGAAATAGCTGAAATCCGCAACAAAGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAAt                                                                                                                          <  1:308147/150‑1 (MQ=255)
                        aTTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAAAGCGATGGATATTGCTGAAATCCGCAACAACGCGGCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAAt                                                                                                                          <  1:1226223/150‑1 (MQ=255)
                         ttGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATa                                                                                                                         <  1:1606969/150‑1 (MQ=255)
                         ttGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATa                                                                                                                         >  2:365358/1‑150 (MQ=255)
                          tGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATac                                                                                                                        <  1:403149/150‑1 (MQ=255)
                             caacctttGTGATTACGGCGGGAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCACCAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACAcc                                                                                                                     <  1:271188/144‑1 (MQ=255)
                             cATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACt                                                                                                                             <  2:1500161/142‑1 (MQ=255)
                             cATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACAcc                                                                                                                     <  2:898389/150‑1 (MQ=255)
                                 cATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGAt                                                                                                                 <  1:1407369/150‑1 (MQ=255)
                                   ttGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGCATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATgc                                                                                                               >  2:470221/1‑150 (MQ=255)
                                    tgtgATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGGCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATgcg                                                                                                              <  1:1195670/150‑1 (MQ=255)
                                       gATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCtt                                                                                                           >  1:506335/1‑150 (MQ=255)
                                         ttACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTg                                                                                                         <  1:87350/150‑1 (MQ=255)
                                         ttACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGAAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTg                                                                                                         <  1:500290/150‑1 (MQ=255)
                                           aCTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTc                                                                                                       <  2:133788/150‑1 (MQ=255)
                                           aCTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTc                                                                                                       <  2:752028/150‑1 (MQ=255)
                                                  tAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCGGCCGGATTTTACGACTAATACACCTGATGCGCTTTGCCACTCAtt                                                                                                >  2:192016/1‑150 (MQ=255)
                                                   aaCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTc                                                                                               <  2:482139/150‑1 (MQ=255)
                                                    aCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCTGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCa                                                                                              <  2:871909/150‑1 (MQ=255)
                                                    aCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCa                                                                                              >  1:870634/1‑150 (MQ=255)
                                                     ccACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAg                                                                                             >  2:565524/1‑150 (MQ=255)
                                                        cTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGGCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGAtt                                                                                          <  2:562275/150‑1 (MQ=255)
                                                          aTCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTca                                                                                        <  2:134148/150‑1 (MQ=255)
                                                          aTCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGAAGCGCTTTGTCACTCATTCAGATTca                                                                                        >  1:78462/1‑150 (MQ=255)
                                                              gTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACAtt                                                                                    >  2:284526/1‑150 (MQ=255)
                                                                    tGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGTCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGCTGCGCTTTGTCACTCCTTCAGATTAACATTCAGCCt                                                                              >  2:1208045/1‑150 (MQ=255)
                                                                     gCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCtt                                                                             >  2:1112065/1‑150 (MQ=255)
                                                                      cGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGCCTGATACCCCGGATGCGCTTTGGCACTCATTCAGATTCACATTCAGCCttt                                                                            >  2:112066/1‑150 (MQ=255)
                                                                       gATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCCGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTc                                                                           <  2:37252/150‑1 (MQ=255)
                                                                        aTGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCa                                                                          <  1:577320/150‑1 (MQ=255)
                                                                         tGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCAc                                                                         <  1:237383/150‑1 (MQ=255)
                                                                           gATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCa                                                                       <  2:1537869/150‑1 (MQ=255)
                                                                             tatTTCTGAAATCATAAACAACGCGTCTCCGGCAGATCCGCTAGTTCATTAATGTGTATGTCCGGCAGAGAGGGGTCTGCCGGATTTTAAGACTTATACAGCTGATGCGCTTTGTCACTCATTCAGTTTCACATTCAGGCTTTCACCATc                                                                     <  2:403117/150‑1 (MQ=255)
                                                                               ttGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCa                                                                   <  2:1792831/150‑1 (MQ=255)
                                                                                    gAAATCCGCAACAACGCGTCTCCGGCAGCTCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCGTTCACCATCCACTACa                                                              >  2:147865/1‑150 (MQ=255)
                                                                                    gAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACa                                                              <  2:1503403/150‑1 (MQ=255)
                                                                                    gAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCAATAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACa                                                              <  1:284447/150‑1 (MQ=255)
                                                                                      aaTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTACGCCTTTCACCATCCACTAAAAg                                                            >  1:1390155/1‑150 (MQ=255)
                                                                                       aTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGa                                                           >  1:204971/1‑150 (MQ=255)
                                                                                       aTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGa                                                           <  1:792238/150‑1 (MQ=255)
                                                                                        tCCGCAACAACGCGTCTCCGTCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGAt                                                          <  1:921716/150‑1 (MQ=255)
                                                                                         ccGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATc                                                         >  1:942876/1‑150 (MQ=255)
                                                                                          cGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATcg                                                        >  2:1431506/1‑150 (MQ=255)
                                                                                           gCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATcgc                                                       >  2:836213/1‑150 (MQ=255)
                                                                                                aacGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCAATACAAGATCCCGCCAc                                                  >  2:367974/1‑150 (MQ=255)
                                                                                                  cgcgTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGc                                                <  2:1777025/150‑1 (MQ=255)
                                                                                                  cgcgTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGc                                                <  2:188075/150‑1 (MQ=255)
                                                                                                  cgcgTCTCCGGCAGATCCGCAACTTCATTAATGAGTATGTCCGACAGAGAGGGCTCTGCAGGATTTTACGACTAAAACAACTGATGCGCTTTGCCACTCATTCAGATTCACCTTCAGCCTTTAACCATCCACTACAAGATCGCGCCACGc                                                >  1:1788545/1‑150 (MQ=255)
                                                                                                   gcgTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCt                                               <  1:502100/150‑1 (MQ=255)
                                                                                                     gTCTCCGGCAGATCCGCAAGTGCATTAATGAGTATGTCCGGCAGAGAGGACTCTGCCGGATTATACGACTAATACAACTGATGCGCTTTGTCACGCATTCAGATTCACATTCCTGGTTTCCCCATCCACTACAAGATCGCGCCAcccaat                                             >  2:106238/1‑145 (MQ=255)
                                                                                                      tctcCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCGCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTa                                            <  1:173835/150‑1 (MQ=255)
                                                                                                        accGGCAGATCCGCAAGTTCATTAATGAGTATGGCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAgg                                          <  2:705893/149‑1 (MQ=255)
                                                                                                         ccGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGc                                         <  2:1378517/150‑1 (MQ=255)
                                                                                                         ccGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTGTAGGc                                         >  2:617980/1‑150 (MQ=255)
                                                                                                          cGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCt                                        <  1:1289763/150‑1 (MQ=255)
                                                                                                             cAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACAACGGATGCGCTTTGTCGCTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCACCGCTTTAGGCTTCg                                     >  2:298159/1‑150 (MQ=255)
                                                                                                               gATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTc                                   <  2:154979/150‑1 (MQ=255)
                                                                                                                 tCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCGGCCGGATTTTACGACTAATACCCCTGATGCGCGTTGTCACTCATTCAGCTTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACCCGTTAGGCTTCGTccc                                 >  1:639433/1‑150 (MQ=255)
                                                                                                                  ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcccc                                <  2:1436665/150‑1 (MQ=255)
                                                                                                                  ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcccc                                <  1:125339/150‑1 (MQ=255)
                                                                                                                   cGCAAGTTCATTAATGAGTATGTCCTGCAGAGAGGGCTCTGCCGGATTTAACGACTAATACTCCTGATGAGCTTTGTCACTCATACAGAATCACATTCAGCCATTCACCATCCACTACAAGATAGCGCCACGCTTTAGGATTCGTaccag                               >  2:107807/1‑148 (MQ=255)
                                                                                                                   cGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCa                               >  1:297272/1‑150 (MQ=255)
                                                                                                                        gTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGtttt                          <  1:778159/150‑1 (MQ=255)
                                                                                                                        gTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGtttt                          <  1:63104/150‑1 (MQ=255)
                                                                                                                          tCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCTCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCAGTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGttttgc                        >  1:262626/1‑148 (MQ=255)
                                                                                                                           cATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCa                       >  2:1667357/1‑150 (MQ=255)
                                                                                                                               aaTGAGTATGTCCGGCAGAGAGGGCTCTGCCGGAGTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGGTTTCCATCCt                   >  1:1120809/1‑150 (MQ=255)
                                                                                                                                  gAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCg                >  2:67130/1‑150 (MQ=255)
                                                                                                                                  gAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCg                >  2:716887/1‑150 (MQ=255)
                                                                                                                                     tATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGTCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAg             >  2:1541190/1‑150 (MQ=255)
                                                                                                                                      aTGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGc            <  2:1367151/150‑1 (MQ=255)
                                                                                                                                        atCCGGCAGAGCCCTCTCTGCCGGATTATACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTGCGCAGCtg          >  1:333942/2‑150 (MQ=255)
                                                                                                                                          ccGGCAGAGCCCTCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGTCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg        >  1:1684190/1‑150 (MQ=255)
                                                                                                                                          ccGGCAGAGCCCTCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg        >  1:1825625/1‑150 (MQ=255)
                                                                                                                                          ccGGCAGAGAGGTCTCTGCCGGATTTTACGACTAATACCCCTGATGCGCGTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCCCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg        <  2:266680/150‑1 (MQ=255)
                                                                                                                                          ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTAAGCCTTTCACCTTCCACTACAAGATCGCGCCACTCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg        <  2:1133428/150‑1 (MQ=255)
                                                                                                                                            ggCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAg      <  1:1796931/150‑1 (MQ=255)
                                                                                                                                             gCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGt     >  1:1278133/1‑150 (MQ=255)
                                                                                                                                              ctgagagGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTa    >  1:1418520/3‑150 (MQ=255)
                                                                                                                                               agagagGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTaa   >  1:219705/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAT  >  NC_000913/3530524‑3530817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: