Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,530,674 G→T 6.1% intergenic (+22/+41) hslO → / ← yhgE heat shock protein Hsp33/DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,530,6740GT 6.1% 443.0 / 10.6 149intergenic (+22/+41)hslO/yhgEheat shock protein Hsp33/DUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (67/71);  new base T (5/5);  total (73/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

GCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCT  >  NC_000913/3530525‑3530823
                                                                                                                                                     |                                                                                                                                                     
gCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAggg                                                                                                                                                       <  2:1715169/150‑1 (MQ=255)
 cATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGc                                                                                                                                                      <  2:598370/150‑1 (MQ=255)
     cTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTg                                                                                                                                                  >  1:1499442/1‑150 (MQ=255)
        gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACTACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCg                                                                                                                                               <  1:341931/150‑1 (MQ=255)
        gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCg                                                                                                                                               <  2:76863/150‑1 (MQ=255)
        gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTAAGTCCGGCAGAGCCCTCTCTGCCg                                                                                                                                               <  1:1145346/150‑1 (MQ=255)
         ctgAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCgg                                                                                                                                              <  1:1777199/148‑1 (MQ=255)
         cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATTTCCGGCAGAGAGGGCTCTGCCgg                                                                                                                                              <  1:1770333/150‑1 (MQ=255)
         cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTg                                                                                                                                                  >  1:749291/1‑146 (MQ=255)
         cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCgg                                                                                                                                              >  1:794075/1‑150 (MQ=255)
         cGGAAGATGGCGAAAGTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATGGCGGAAAGCCGCCACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCgg                                                                                                                                              <  1:989803/150‑1 (MQ=255)
         cGGAAGAAGGCGAAATTGACATGCATTGTGATTACTGCCGTCACCACTAGCTGTTCAATGCGATGGATCTAGCTGACATCCGCAACAACGCGTCTCCGGCAGATCCGCAATTTCATTAATGAGTATGTCCGGCAGAGAGTGCTCTGCCgg                                                                                                                                              <  2:1478234/150‑1 (MQ=255)
          ggAAGATGGCGAAATTGCGACGCAGTGAGATTACTGCGGCTACCACTATCTGTTCAATGCGCTGGATTTTGCTGAAATCCGCAACTACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCGGa                                                                                                                                             <  2:297194/150‑1 (MQ=255)
                tGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTAc                                                                                                                                       >  2:1289763/1‑150 (MQ=255)
                tGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTAc                                                                                                                                       >  2:1571648/1‑150 (MQ=255)
                tGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAAGGCGATGGATATTGCTGAAATCAGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTATTGAGTATGTCCTGCAGAGAGGGCTCTGCCGGATATTAc                                                                                                                                       >  1:260516/1‑150 (MQ=255)
                 ggCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACg                                                                                                                                      <  1:1076117/150‑1 (MQ=255)
                  gCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGCTCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGa                                                                                                                                     >  2:539897/1‑150 (MQ=255)
                  gCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGa                                                                                                                                     >  1:1394655/1‑150 (MQ=255)
                  gCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGAGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATTTCCGGCAGAGAGGGCTCTGCCGGATTTTACGa                                                                                                                                     >  2:125339/1‑150 (MQ=255)
                     aaaTTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTa                                                                                                                                  >  2:67166/1‑150 (MQ=255)
                       aTTTACATGCATTGTGCTTACTGCGGTAACCACTACCTGGTCAATGCGATGGAAATAGCTGAAATCCGCAACAAAGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAAt                                                                                                                                <  1:308147/150‑1 (MQ=255)
                       aTTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAAAGCGATGGATATTGCTGAAATCCGCAACAACGCGGCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAAt                                                                                                                                <  1:1226223/150‑1 (MQ=255)
                        ttGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATa                                                                                                                               <  1:1606969/150‑1 (MQ=255)
                         tGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATac                                                                                                                              <  1:403149/150‑1 (MQ=255)
                            caacctttGTGATTACGGCGGGAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCACCAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACAcc                                                                                                                           <  1:271188/144‑1 (MQ=255)
                            cATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACt                                                                                                                                   >  1:1500161/1‑142 (MQ=255)
                            cATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACt                                                                                                                                   <  2:1500161/142‑1 (MQ=255)
                            cATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACAcc                                                                                                                           <  2:898389/150‑1 (MQ=255)
                                  ttGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGCATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATgc                                                                                                                     >  2:470221/1‑150 (MQ=255)
                                      gATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCtt                                                                                                                 >  1:506335/1‑150 (MQ=255)
                                      gATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCtt                                                                                                                 >  1:1422856/1‑150 (MQ=255)
                                        ttACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTg                                                                                                               <  1:87350/150‑1 (MQ=255)
                                        ttACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGAAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTg                                                                                                               <  1:500290/150‑1 (MQ=255)
                                          aCTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTc                                                                                                             <  2:752028/150‑1 (MQ=255)
                                                gTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCAt                                                                                                       >  2:1749219/1‑150 (MQ=255)
                                                 tAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCGGCCGGATTTTACGACTAATACACCTGATGCGCTTTGCCACTCAtt                                                                                                      >  2:192016/1‑150 (MQ=255)
                                                  aaCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTc                                                                                                     <  1:1610592/150‑1 (MQ=255)
                                                   aCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCa                                                                                                    >  1:870634/1‑150 (MQ=255)
                                                      aCTATCTGTTCAATGCGTTGGATATTGCGTAAATCCGCAACAACGCGTCTCCGGCAGATCCGCATGTTCATTAATGAGTTTTTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTATTACACCTGATGCGCTTTGTCACTCATTCAGAt                                                                                                 <  2:246759/150‑1 (MQ=255)
                                                      aCTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGAt                                                                                                 <  2:386345/150‑1 (MQ=255)
                                                       cTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGGATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGAtt                                                                                                <  2:1364350/150‑1 (MQ=255)
                                                       cTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGGCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGAtt                                                                                                <  2:562275/150‑1 (MQ=255)
                                                         aTCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTca                                                                                              <  2:134148/150‑1 (MQ=255)
                                                         aTCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGAAGCGCTTTGTCACTCATTCAGATTca                                                                                              >  1:78462/1‑150 (MQ=255)
                                                             gTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCGGGAGTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCCCAtt                                                                                          >  2:1619931/1‑150 (MQ=255)
                                                                cAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAg                                                                                       >  2:738721/1‑150 (MQ=255)
                                                                   tGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGTCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGCTGCGCTTTGTCACTCCTTCAGATTAACATTCAGCCt                                                                                    >  2:1208045/1‑150 (MQ=255)
                                                                    gCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCtt                                                                                   >  2:1112065/1‑150 (MQ=255)
                                                                     cGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGCCTGATACCCCGGATGCGCTTTGGCACTCATTCAGATTCACATTCAGCCttt                                                                                  >  2:112066/1‑150 (MQ=255)
                                                                     cGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCttt                                                                                  >  1:1250697/1‑150 (MQ=255)
                                                                       aTGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCa                                                                                <  1:577320/150‑1 (MQ=255)
                                                                        tGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCAc                                                                               <  2:870634/150‑1 (MQ=255)
                                                                        tGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCAc                                                                               <  1:237383/150‑1 (MQ=255)
                                                                            tatTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATc                                                                           <  2:1406738/150‑1 (MQ=255)
                                                                              ttGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCa                                                                         <  2:1792831/150‑1 (MQ=255)
                                                                                   gAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCAATAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACa                                                                    <  1:284447/150‑1 (MQ=255)
                                                                                     aaTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTACGCCTTTCACCATCCACTAAAAg                                                                  >  1:1390155/1‑150 (MQ=255)
                                                                                      aTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGa                                                                 >  1:204971/1‑150 (MQ=255)
                                                                                      aTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGa                                                                 <  1:792238/150‑1 (MQ=255)
                                                                                       tCCGCAACAACGCGTCTCCGTCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGAt                                                                <  1:921716/150‑1 (MQ=255)
                                                                                       tCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGAt                                                                >  1:1797628/1‑150 (MQ=255)
                                                                                        ccGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATc                                                               >  1:942876/1‑150 (MQ=255)
                                                                                          gCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATcgc                                                             >  2:836213/1‑150 (MQ=255)
                                                                                               aacGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCAATACAAGATCCCGCCAc                                                        >  2:367974/1‑150 (MQ=255)
                                                                                                 cgcgTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGc                                                      <  2:188075/150‑1 (MQ=255)
                                                                                                  gcgTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCt                                                     <  1:502100/150‑1 (MQ=255)
                                                                                                    gTCTCCGGCAGATCCGCAAGTGCATTAATGAGTATGTCCGGCAGAGAGGACTCTGCCGGATTATACGACTAATACAACTGATGCGCTTTGTCACGCATTCAGATTCACATTCCTGGTTTCCCCATCCACTACAAGATCGCGCCAcccaat                                                   >  2:106238/1‑145 (MQ=255)
                                                                                                       tcCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAgg                                                <  2:1520354/150‑1 (MQ=255)
                                                                                                       accGGCAGATCCGCAAGTTCATTAATGAGTATGGCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAgg                                                <  2:705893/149‑1 (MQ=255)
                                                                                                        ccGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGc                                               >  2:366046/1‑150 (MQ=255)
                                                                                                        ccGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGc                                               <  2:1378517/150‑1 (MQ=255)
                                                                                                        ccGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTGTAGGc                                               >  2:617980/1‑150 (MQ=255)
                                                                                                            cAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACAACGGATGCGCTTTGTCGCTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCACCGCTTTAGGCTTCg                                           >  2:298159/1‑150 (MQ=255)
                                                                                                                tCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCGGCCGGATTTTACGACTAATACCCCTGATGCGCGTTGTCACTCATTCAGCTTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACCCGTTAGGCTTCGTccc                                       >  1:639433/1‑150 (MQ=255)
                                                                                                                 ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcccc                                      <  2:1436665/150‑1 (MQ=255)
                                                                                                                 ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcccc                                      <  1:125339/150‑1 (MQ=255)
                                                                                                                 ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACGACAAGATCGCGCCACGCTTTAGGCTTCGTcccc                                      >  2:1500198/1‑150 (MQ=255)
                                                                                                                  cGCAAGTTCATTAATGAGTATGTCCTGCAGAGAGGGCTCTGCCGGATTTAACGACTAATACTCCTGATGAGCTTTGTCACTCATACAGAATCACATTCAGCCATTCACCATCCACTACAAGATAGCGCCACGCTTTAGGATTCGTaccag                                     >  2:107807/1‑148 (MQ=255)
                                                                                                                  cGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCa                                     >  1:1723269/1‑150 (MQ=255)
                                                                                                                  cGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCa                                     >  1:297272/1‑150 (MQ=255)
                                                                                                                       gTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGtttt                                <  1:63104/150‑1 (MQ=255)
                                                                                                                        ttCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGCTGCGCGTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGAGCGCGCCACGCTGTAGGCTTCGTCCCCAGTTTTc                               <  1:210294/150‑1 (MQ=255)
                                                                                                                         tCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCTCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCAGTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGttttgc                              >  1:262626/1‑148 (MQ=255)
                                                                                                                              aaTGAGTATGTCCGGCAGAGAGGGCTCTGCCGGAGTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGGTTTCCATCCt                         >  1:1120809/1‑150 (MQ=255)
                                                                                                                                 gAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCg                      <  1:757400/150‑1 (MQ=255)
                                                                                                                                  aGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGc                     <  1:1289094/150‑1 (MQ=255)
                                                                                                                                    tATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGTCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAg                   >  2:1541190/1‑150 (MQ=255)
                                                                                                                                       atCCGGCAGAGCCCTCTCTGCCGGATTATACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTGCGCAGCtg                >  1:333942/2‑150 (MQ=255)
                                                                                                                                         ccGGCAGAGCCCTCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTATACCATCCGTCGCAGCtgtg              >  1:1224624/1‑150 (MQ=255)
                                                                                                                                         ccGGCAGAGAGGTCTCTGCCGGATTTTACGACTAATACCCCTGATGCGCGTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCCCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg              <  2:266680/150‑1 (MQ=255)
                                                                                                                                         ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg              <  2:1548847/150‑1 (MQ=255)
                                                                                                                                         ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTAAGCCTTTCACCTTCCACTACAAGATCGCGCCACTCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg              <  2:1133428/150‑1 (MQ=255)
                                                                                                                                           ggCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAg            <  2:41344/150‑1 (MQ=255)
                                                                                                                                           ggCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAg            <  1:1796931/150‑1 (MQ=255)
                                                                                                                                             ctgagagGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTa          >  1:1418520/3‑150 (MQ=255)
                                                                                                                                               gagagGGCGCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAt        <  2:899820/150‑1 (MQ=255)
                                                                                                                                                    ggCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTtttc   <  2:1336391/150‑1 (MQ=255)
                                                                                                                                                     gCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTtttct  <  2:936126/150‑1 (MQ=255)
                                                                                                                                                     gCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTAGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTAAGGCTTCGTCCCCAGATTTCCATCCTTCGCAGCTGAGAGTAAATattct  >  1:1154884/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCT  >  NC_000913/3530525‑3530823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: