Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,570,891 C→T 5.3% P143P (CCG→CCA glgX ← glycogen debranching enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,570,8910CT 5.3% 351.2 / 4.3 112P143P (CCG→CCAglgXglycogen debranching enzyme
Reads supporting (aligned to +/- strand):  ref base C (54/52);  new base T (4/2);  total (58/54)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCAATTTGCCGCGCGCAAGGAT  >  NC_000913/3570744‑3571039
                                                                                                                                                   |                                                                                                                                                    
gCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGcggcgg                                                                                                                                                    >  1:1452422/1‑150 (MQ=255)
 cccAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCggg                                                                                                                                                   >  2:263424/1‑150 (MQ=255)
 cccAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCggg                                                                                                                                                   <  2:144764/150‑1 (MQ=255)
  ccAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCgggg                                                                                                                                                  >  2:797821/1‑150 (MQ=255)
  ccAATTGTTTCAAATAGTTGAGCATCACCGGATGCCCGACGGCTTTATAAGTGCCACGGAGCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCGTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCgggg                                                                                                                                                  <  2:1581117/150‑1 (MQ=255)
   cAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGc                                                                                                                                                 >  1:560893/1‑150 (MQ=255)
   cAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGc                                                                                                                                                 <  2:619218/150‑1 (MQ=255)
    aaTTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGca                                                                                                                                                <  1:1729897/150‑1 (MQ=255)
     aTTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGATAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGcag                                                                                                                                               <  1:1419101/150‑2 (MQ=255)
     aTTGTTGCAAATACTTGAGCCTCCCCGGCTGCCCGAGGGCCTTCTAAGTGCCCCGGAGCTCGCCCGGGATCTCCGGGGGCACGTCCGGTCATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGcat                                                                                                                                               <  1:1681917/150‑1 (MQ=255)
      ttGTTTCAAATAGTTGATCCTCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGAGCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGcatc                                                                                                                                              <  1:1669647/150‑1 (MQ=255)
      ttGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGcatc                                                                                                                                              <  2:1807791/150‑1 (MQ=255)
      ttGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGcatc                                                                                                                                              <  1:840304/150‑1 (MQ=255)
       tGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGcatca                                                                                                                                             <  1:922901/150‑1 (MQ=255)
       tGTTTCAAATAGTGGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGgcagca                                                                                                                                             <  2:1534151/150‑1 (MQ=255)
          ttCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCt                                                                                                                                          >  2:1634560/1‑150 (MQ=255)
          ttCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCt                                                                                                                                          >  1:1779117/1‑150 (MQ=255)
             aaaTAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCTCGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTcc                                                                                                                                       <  2:627373/150‑1 (MQ=255)
             aaaTAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCGTCATCTTcc                                                                                                                                       >  1:1203428/1‑150 (MQ=255)
             aaaTAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTcc                                                                                                                                       <  2:1393435/150‑1 (MQ=255)
             aaaTAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTcc                                                                                                                                       >  1:462431/1‑150 (MQ=255)
             aaaTAGTTGATCATCACCGGATGCCCGAGGGCTTAATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTcc                                                                                                                                       <  1:492375/150‑1 (MQ=255)
              aaTAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTccc                                                                                                                                      >  2:840064/1‑150 (MQ=255)
                tAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAg                                                                                                                                    <  2:1309002/150‑1 (MQ=255)
                tAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAg                                                                                                                                    >  1:118769/1‑150 (MQ=255)
                tAGTTGATCATCACCGGATGCACGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAg                                                                                                                                    <  2:895539/150‑1 (MQ=255)
                     gATCATCCCCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATa                                                                                                                               >  2:1252682/1‑150 (MQ=255)
                     gATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATa                                                                                                                               >  2:45486/1‑150 (MQ=255)
                      atcatcACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAg                                                                                                                              <  2:819033/150‑1 (MQ=255)
                        catcaCCGGATGCACGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTg                                                                                                                            <  1:1634386/150‑1 (MQ=255)
                          tcaCCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGAt                                                                                                                          >  2:305073/1‑150 (MQ=255)
                           caCCGGATGCCCGAGGGCTTTATAAGTGCCCCGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATc                                                                                                                         <  2:645974/150‑1 (MQ=255)
                            aCCGGATGCCCGAGGGCTTTATAAGTTCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCa                                                                                                                        <  2:1305272/150‑1 (MQ=255)
                               ggATGCCCGAGGGGTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAacc                                                                                                                     <  2:331543/150‑1 (MQ=255)
                                  tGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAaccacc                                                                                                                  <  1:1323089/150‑1 (MQ=255)
                                  tGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAaccacc                                                                                                                  <  1:115893/150‑1 (MQ=255)
                                  tGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAaccacc                                                                                                                  <  1:1060700/150‑1 (MQ=255)
                                    cccGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACcaccac                                                                                                                >  2:156244/1‑150 (MQ=255)
                                     ccGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCACCCACCCCt                                                                                                               >  2:1433889/1‑150 (MQ=255)
                                           gCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCAt                                                                                                         >  1:1549820/1‑150 (MQ=255)
                                            cTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGGAACTACGTTAATCCTTTGACATGGTATTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCTTCTTGCGAGTCATAGTGGTCGGCCCCCAGTACGCAtt                                                                                                        >  1:298507/1‑150 (MQ=255)
                                            cTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCAtt                                                                                                        >  2:1318456/1‑150 (MQ=255)
                                               tataAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCg                                                                                                     >  1:1350377/1‑150 (MQ=255)
                                                ataAGTGCCACGGATCTCGACCGGGATCTCCGGGGGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCgg                                                                                                    <  2:1393266/150‑1 (MQ=255)
                                                   aGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGgcgc                                                                                                 <  1:49167/150‑1 (MQ=255)
                                                    gTGCCACGGATCTCGCCCGGGATCTCCGGGTGAAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTTCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCa                                                                                                >  1:1172794/1‑150 (MQ=255)
                                                       ccACGGATCTCGACCGGGATCTCCGGGTGCAAGTACATTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATg                                                                                             >  1:1461316/1‑150 (MQ=255)
                                                          cGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTGCCCAGTCATTGTGATCAACCACCACTACGCATTTCGGCGCAATggcg                                                                                          >  2:19352/1‑150 (MQ=255)
                                                           ggATCTCGACCGGGATCTCCGGGTGCAAGTAGGTTAATCCTTTGTCATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTACCAGTCATAGTGATCAACCACCACTAGGCATTTCGGCGCAATggcgg                                                                                         <  2:1041473/150‑1 (MQ=255)
                                                                tcGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTg                                                                                    >  1:258301/1‑150 (MQ=255)
                                                                   aCCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGTCGTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTcg                                                                                 <  1:78694/150‑1 (MQ=255)
                                                                    ccGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTcgc                                                                                <  1:515799/150‑1 (MQ=255)
                                                                      gggATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGa                                                                              >  1:88862/1‑150 (MQ=255)
                                                                         aTCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGTTCAACCACCACTACGAATTTCGGCGCATTGGCGGCGTTGTCGAGCTAg                                                                           >  1:287465/1‑150 (MQ=255)
                                                                          tctcCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGt                                                                          >  2:1342936/1‑150 (MQ=255)
                                                                           ctcCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTc                                                                         >  2:470838/1‑150 (MQ=255)
                                                                            tcCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGCGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCa                                                                        <  1:136287/150‑1 (MQ=255)
                                                                             ccGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAg                                                                       <  2:541921/150‑1 (MQ=255)
                                                                                 gTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTc                                                                   >  1:1301398/1‑150 (MQ=255)
                                                                                   gCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCAt                                                                 <  2:48527/150‑1 (MQ=255)
                                                                                    cAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTCCGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCAtt                                                                >  1:1168670/1‑150 (MQ=255)
                                                                                    cAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCAtt                                                                <  2:649291/150‑1 (MQ=255)
                                                                                    cAAGTACGTTAATCCTTTGACATGGGCATCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCAtt                                                                <  2:556271/150‑1 (MQ=255)
                                                                                     aaGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTa                                                               >  2:733458/1‑150 (MQ=255)
                                                                                     aaGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGGGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTa                                                               <  1:515619/150‑1 (MQ=255)
                                                                                      aGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTAt                                                              >  1:1269053/1‑150 (MQ=255)
                                                                                      aGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTAt                                                              >  1:1551348/1‑150 (MQ=255)
                                                                                       gTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATg                                                             <  2:1390007/150‑1 (MQ=255)
                                                                                         aCGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGAc                                                           <  1:407036/150‑1 (MQ=255)
                                                                                              aaTCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCg                                                      <  1:460295/150‑1 (MQ=255)
                                                                                                  cTTTGACATGGGCTGCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTgca                                                  <  2:868156/150‑1 (MQ=255)
                                                                                                          tGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCggg                                          <  1:743665/150‑1 (MQ=255)
                                                                                                          tGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCggg                                          <  1:1664758/150‑1 (MQ=255)
                                                                                                           gggCTTCATAAATGTTGGTGCTGCCGCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGAACAACCACCACTACGCATTTCGGCGCCATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGt                                         <  1:285105/150‑1 (MQ=255)
                                                                                                           gggCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGt                                         <  1:1108035/150‑1 (MQ=255)
                                                                                                             gCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCAGTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCAGTATGACCGGCGTGCAGCAGCGGGttt                                       >  1:984638/1‑149 (MQ=255)
                                                                                                                tCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCt                                    <  1:446115/150‑1 (MQ=255)
                                                                                                                 cgtAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCtt                                   >  2:1005089/3‑150 (MQ=255)
                                                                                                                 aaTAAATGATGGTGCTGCCCCAAGGCGTGCGGGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCtt                                   >  2:669670/2‑150 (MQ=255)
                                                                                                                   tctatgatgGAGCTGCCCCACGGCGTGCGCGTCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACGACTCCGCCTTTCGGCGCAAGGGCGGCGTTGTCGCGATAGTAAGGGTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTa                                 <  1:263424/147‑1 (MQ=255)
                                                                                                                   tAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATGAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATGATGACCGGCGTGCAGCAGCGGGTTATCTTTa                                 >  1:277192/1‑150 (MQ=255)
                                                                                                                     aaTGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTATaaa                               >  2:170728/1‑150 (MQ=255)
                                                                                                                           ggTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGAAAGTCAGGTTCATTAAGACCGGCGTGCAGCAGCGGGTTAGCTTTAAACTccgc                         >  2:1116271/1‑148 (MQ=255)
                                                                                                                           gatgatgcCCCGCCGCGCACGCCGTGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTcccc                         >  2:782758/6‑150 (MQ=255)
                                                                                                                            gTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCg                        >  1:288803/1‑150 (MQ=255)
                                                                                                                            atgatgcCCCGCCGCGCACGCCGTGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCg                        >  1:214888/5‑150 (MQ=255)
                                                                                                                             tgctgcCCCGCCGCGCACGCCGTGGGGCATCATCTTCCCAGGCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTTTCGCGATAGGCAGGTGCATTATGCCCGGCGTGCAGCAGCGGGGTATCTTTATACTCcgcgg                       >  2:259712/1‑149 (MQ=255)
                                                                                                                             tgatgcCCCGCCGCGCACGCCGTGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGt                       >  2:1255488/4‑150 (MQ=255)
                                                                                                                              gctgcCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTc                      >  1:1163823/1‑150 (MQ=255)
                                                                                                                              gctgcCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTc                      >  1:1236255/1‑150 (MQ=255)
                                                                                                                              gctgcCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTc                      >  2:1217852/1‑150 (MQ=255)
                                                                                                                                tgcCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCaa                    <  2:1806730/150‑1 (MQ=255)
                                                                                                                                tgcCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCaa                    >  2:1578495/1‑150 (MQ=255)
                                                                                                                                   cccACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATATTTAAACTCCCCGTAAAttt                 >  2:1695090/1‑150 (MQ=255)
                                                                                                                                    ccACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCAATTTg                >  2:447862/1‑150 (MQ=255)
                                                                                                                                      aCGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCAATTTGcc              <  2:1350377/150‑1 (MQ=255)
                                                                                                                                            tGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCAATTTGCCgcgcgc        >  1:1066245/1‑150 (MQ=255)
                                                                                                                                            tGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCAATATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCAATTTGCCgcgcgc        >  1:581445/1‑150 (MQ=255)
                                                                                                                                                 ggcggGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCAATTTGCCGCGCGCAAGGa   >  1:593255/1‑150 (MQ=255)
                                                                                                                                                  gcggGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTGCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCAATTTGCCGCGCGCAAGGat  <  2:258301/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCACGGCGTGCGCGGCGGGGCATCATCTTCCCAGTCATAGTGATCAACCACCACTACGCATTTCGGCGCAATGGCGGCGTTGTCGCGATAGTCAGGTTCATTATGACCGGCGTGCAGCAGCGGGTTATCTTTAAACTCCCCGTCAATTTGCCGCGCGCAAGGAT  >  NC_000913/3570744‑3571039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: