Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,600,995 C→T 6.2% intergenic (‑212/+33) rpoH ← / ← ftsX RNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,600,9950CT 6.2% 408.6 / 9.5 129intergenic (‑212/+33)rpoH/ftsXRNA polymerase, sigma 32 (sigma H) factor/putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (58/62);  new base T (6/2);  total (65/64)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 130 total aligned reads displayed.

AGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGAT  >  NC_000913/3600849‑3601144
                                                                                                                                                  |                                                                                                                                                     
agCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCa                                                                                                                                                    <  1:1813457/150‑1 (MQ=255)
  cAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCAtt                                                                                                                                                  >  1:1069437/1‑150 (MQ=255)
   aaCGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACACACAAAATGTGACATAGAGATGAATTACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTg                                                                                                                                                 <  1:297685/150‑1 (MQ=255)
     cGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCa                                                                                                                                               <  2:1787621/150‑1 (MQ=255)
        ggTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAggg                                                                                                                                            <  2:1157476/150‑1 (MQ=255)
         gTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAAACCATTGCAGGGa                                                                                                                                           >  1:1735452/1‑150 (MQ=255)
         gTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTAGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGa                                                                                                                                           <  2:1196783/150‑1 (MQ=255)
           tATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGaaa                                                                                                                                         <  2:919472/150‑1 (MQ=255)
           tATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGaaa                                                                                                                                         >  2:375832/1‑150 (MQ=255)
           tATCATTCACTGTTTTATCAGACCGTGATTTTATCCAAAAGTTCTATGCAAGCTTGTGAATTAATTTCGCACAAAATGTGTCATAGAGATGAATTACAGGGAATAGACAACGGGGTCGCTTTCCCTGCAATGGGTTCCGTAGCAGGGaaa                                                                                                                                         <  1:346020/150‑1 (MQ=255)
            aTCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAag                                                                                                                                        <  2:1320772/150‑1 (MQ=255)
            aTCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCAATGGGTTCCGTAGCAGGGAAag                                                                                                                                        <  2:1666355/150‑1 (MQ=255)
              cATTCACTGTTTTATCAGACAGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGAAATAGACATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAagag                                                                                                                                      >  1:497458/1‑150 (MQ=255)
                ttCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGta                                                                                                                                    <  1:300849/150‑1 (MQ=255)
                 tCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGtat                                                                                                                                   <  1:1431211/150‑1 (MQ=255)
                  cACTGTTATATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGAGGAAATACCGGGAAGAGACAACGGTGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGtata                                                                                                                                  >  1:286383/1‑150 (MQ=255)
                   aCTGTTTTATCAGACCGTGATTTTATCCACAAGATCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGATACGGAACACATTGCAGGGAAAGAGTATaa                                                                                                                                 >  2:1191433/1‑150 (MQ=255)
                    cTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAac                                                                                                                                >  1:723189/1‑150 (MQ=255)
                     tGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAaca                                                                                                                               <  1:1514814/150‑1 (MQ=255)
                      gTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAacac                                                                                                                              >  1:1194600/1‑150 (MQ=255)
                         ttATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCt                                                                                                                           <  1:947429/150‑1 (MQ=255)
                              aGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTttat                                                                                                                      >  1:176582/1‑150 (MQ=255)
                              aGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTttat                                                                                                                      >  2:701504/1‑150 (MQ=255)
                                 ccGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTtattat                                                                                                                   <  2:1351581/150‑1 (MQ=255)
                                  cGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTattatt                                                                                                                  <  1:973978/150‑1 (MQ=255)
                                   gTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTattatta                                                                                                                 >  1:1295521/1‑149 (MQ=255)
                                   gTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTc                                                                                                                 <  1:1217484/150‑1 (MQ=255)
                                    tGATTTTATCCACAAGTTCAATGCAAGTTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGTGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCa                                                                                                                <  2:13547/150‑1 (MQ=255)
                                     taTTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAg                                                                                                               <  1:728445/149‑1 (MQ=255)
                                      aTTTTATCCACAAGTTCAATTCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAgg                                                                                                              <  1:519392/150‑1 (MQ=255)
                                      aTTTTATACACAAGTTCCATGCAAGCTTGTGAATAAATTACGCACAAAAGGTGACATAGAGATGAAATACCGTGAAGAGACAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAgg                                                                                                              <  2:1268567/150‑1 (MQ=255)
                                         ttATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGt                                                                                                           <  2:839179/150‑1 (MQ=255)
                                          tATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTa                                                                                                          >  2:1026339/1‑150 (MQ=255)
                                            tCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTaaa                                                                                                        >  2:1287858/1‑150 (MQ=255)
                                             ccACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAg                                                                                                       >  1:25132/1‑150 (MQ=255)
                                              cacaAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGt                                                                                                      >  2:863053/1‑150 (MQ=255)
                                                caAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTgg                                                                                                    <  1:750792/150‑1 (MQ=255)
                                                   gTTCAATGCAAGCTTGTGAATAAATTCCGCACAAAATGGGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGt                                                                                                 <  2:734788/150‑1 (MQ=255)
                                                   gTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGt                                                                                                 >  1:1099294/1‑150 (MQ=255)
                                                        aTGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGTAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtg                                                                                            <  2:137702/150‑1 (MQ=255)
                                                         tGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgt                                                                                           >  1:1770781/1‑150 (MQ=255)
                                                          gCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgtt                                                                                          >  2:786594/1‑150 (MQ=255)
                                                          gCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgtt                                                                                          <  2:944668/150‑1 (MQ=255)
                                                           cAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgttg                                                                                         <  2:442619/150‑1 (MQ=255)
                                                           cAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgttg                                                                                         <  2:1069437/150‑1 (MQ=255)
                                                           cAAGATTGTGAATAAATAACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGTCAACGGGGTCTCTTTCCCTGCTACGGATCCCATTGCAGTGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAATGTGGCGTAAAtgttg                                                                                         <  1:1191433/150‑1 (MQ=255)
                                                            aaGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgttgt                                                                                        >  2:802411/1‑150 (MQ=255)
                                                            aaGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAAtgttgt                                                                                        <  1:1183434/150‑1 (MQ=255)
                                                                ttGTGAACAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTATTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTAAGGCGTAAAGTTTCGTAAATGTTGTACCg                                                                                    >  1:773396/1‑150 (MQ=255)
                                                                   tgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTgg                                                                                 >  2:1619857/1‑150 (MQ=255)
                                                                   tgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTgg                                                                                 <  1:253390/150‑1 (MQ=255)
                                                                   tgAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGACACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCATTGAAATAGTAAAACACGCTTTTATTATTCAGGCGGAAAGTGGCGTAAATGTTGTACCGTgg                                                                                 <  2:280389/150‑1 (MQ=255)
                                                                             aCGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGc                                                                       <  1:199339/150‑1 (MQ=255)
                                                                              cGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCt                                                                      >  2:1240382/1‑150 (MQ=255)
                                                                               gCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTg                                                                     <  2:1303780/150‑1 (MQ=255)
                                                                               ccacaAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTg                                                                     <  2:463936/149‑1 (MQ=255)
                                                                                cacaAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTTAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGc                                                                    >  1:763208/1‑150 (MQ=255)
                                                                                cacaAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGc                                                                    >  2:1199078/1‑150 (MQ=255)
                                                                                       tgtgACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAg                                                             <  2:135591/150‑1 (MQ=255)
                                                                                          gACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGAGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCa                                                          >  2:1065384/1‑150 (MQ=255)
                                                                                                 agaTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCg                                                   <  2:746082/150‑1 (MQ=255)
                                                                                                   aTGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAg                                                 <  1:1512804/150‑1 (MQ=255)
                                                                                                    tGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGc                                                >  1:384940/1‑150 (MQ=255)
                                                                                                    tGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGc                                                >  1:1651745/1‑150 (MQ=255)
                                                                                                    tGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGc                                                >  1:1374838/1‑150 (MQ=255)
                                                                                                    tGAAATACCGGGAAGAGACAAAGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCCAGCCACGCTGCCACCCAGCCAATCATCCAGc                                                >  2:136482/1‑150 (MQ=255)
                                                                                                          aCCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACCCGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATCTTGTACCGTGGCCAGCCACGCTGACCCCCAGCCAATCATCGAGCATACca                                          >  2:330032/1‑150 (MQ=255)
                                                                                                          aCCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACca                                          <  1:735987/150‑1 (MQ=255)
                                                                                                           ccGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACcag                                         >  2:1199300/1‑150 (MQ=255)
                                                                                                             gggAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACcagca                                       >  2:1485121/1‑150 (MQ=255)
                                                                                                               gAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCagcagc                                     <  1:1089580/150‑1 (MQ=255)
                                                                                                               gAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGAATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCagcagc                                     >  1:522089/1‑150 (MQ=255)
                                                                                                                 agagACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCaa                                   <  2:1304712/150‑1 (MQ=255)
                                                                                                                  gagaCAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCATGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGATGCCACCCATCCAATAAGAGAGAATACCAGCAGCAAt                                  >  2:93156/1‑150 (MQ=255)
                                                                                                                   agaCAACGGTGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATa                                 <  2:1736762/150‑1 (MQ=255)
                                                                                                                      cAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCa                              <  2:689066/150‑1 (MQ=255)
                                                                                                                      cAACGGGGGCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGGGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCa                              <  2:1706635/150‑1 (MQ=255)
                                                                                                                       aaCGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAg                             <  2:204497/150‑1 (MQ=255)
                                                                                                                        aCGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAgg                            <  1:1273546/150‑1 (MQ=255)
                                                                                                                            ggTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGTCGTAAAGTTGCGTAAATGTTGTACCGTGGCAAGCCAAGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCAtt                        <  2:286383/150‑1 (MQ=255)
                                                                                                                            atactctTTCCCTGCAATGGGTTCCGTAGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCAtt                        >  2:394828/4‑150 (MQ=38)
                                                                                                                              tctcTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCa                      <  2:885453/150‑1 (MQ=255)
                                                                                                                               ctctTTCCCTGCTACGGAACCCATTGCAGGGTAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACAGTGGCAAGCCACGCTGCCACCCAGCCACTCATCGAGCATACCAGCAGCAATAGCAGGCATTCAt                     >  1:45390/1‑150 (MQ=255)
                                                                                                                               ctctTTCCCTGCAATGGGTTCCGTAGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCAt                     >  2:150487/1‑150 (MQ=39)
                                                                                                                                tctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCACGCCACGCTGCCACCCAGCCACTCATCGAGCATACCAGCAGCAATAGCAGGCATTCATc                    >  1:767457/1‑150 (MQ=255)
                                                                                                                                tctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATc                    <  2:513865/150‑1 (MQ=255)
                                                                                                                                tctTTCCCTGCAATGGGTTCCGTAGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATc                    >  1:1332484/1‑150 (MQ=39)
                                                                                                                                 ctTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTCCCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCGTACCTGCAGCAATAGCAGGCATTCATCg                   >  1:1132172/1‑150 (MQ=255)
                                                                                                                                 ctTTCCCTGCAATGGGTTCCGTAGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCg                   >  2:1508648/1‑150 (MQ=255)
                                                                                                                                  tttCCCTGCAATGGGTTCCGTAGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGa                  >  1:372465/1‑150 (MQ=255)
                                                                                                                                      ccTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGa              >  2:862323/1‑150 (MQ=255)
                                                                                                                                      ccTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGa              >  2:1940/1‑150 (MQ=255)
                                                                                                                                       cTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGAt             >  1:1703161/1‑150 (MQ=255)
                                                                                                                                       cTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGAt             >  1:1092682/1‑150 (MQ=255)
                                                                                                                                              ggAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCAAt      >  1:1100355/1‑150 (MQ=255)
                                                                                                                                               gAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGAGTAAATGTTGTACCGTGGCAAGCCACGCAGCCACACAGCCAATCATCGAGCATACCAGCAGCAAAATCATGCATTCATCCAATGATAAGCCaat     >  1:516182/1‑148 (MQ=255)
                                                                                                                                                aaCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTg    <  2:367180/150‑1 (MQ=255)
                                                                                                                                                aaCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGACAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTg    >  2:1227422/1‑150 (MQ=255)
                                                                                                                                                 aCCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGa   <  2:666854/150‑1 (MQ=255)
                                                                                                                                                  cccATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGat  >  2:1374307/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AGCAACGAGGTTATCATTCACTGTTTTATCAGACCGTGATTTTATCCACAAGTTCAATGCAAGCTTGTGAATAAATTACGCACAAAATGTGACATAGAGATGAAATACCGGGAAGAGACAACGGGGTCTCTTTCCCTGCTACGGAACCCATTGCAGGGAAAGAGTATAACACGCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGAT  >  NC_000913/3600849‑3601144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: