Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,616,399 T→G 5.9% V73G (GTG→GGG)  nikC → nickel ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,616,3990TG 5.9% 405.8 / 7.4 155V73G (GTG→GGG) nikCnickel ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (72/70);  new base G (4/7);  total (78/77)
Fisher's exact test for biased strand distribution p-value = 5.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

GATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGT  >  NC_000913/3616250‑3616548
                                                                                                                                                     |                                                                                                                                                     
gATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCGCGCGGCTGATGGCAGCGACCCGCgt                                                                                                                                                       >  2:927426/1‑150 (MQ=255)
 aTTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCgtg                                                                                                                                                      <  2:1653074/150‑1 (MQ=255)
   tGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTc                                                                                                                                                    >  1:682593/1‑150 (MQ=255)
     cgcTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCCGCGACCCGCGTGTCGc                                                                                                                                                  >  1:1164461/1‑150 (MQ=255)
     cgcTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGGGTCGc                                                                                                                                                  >  2:639474/1‑150 (MQ=255)
       cTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtc                                                                                                                                                <  2:449540/150‑1 (MQ=255)
       cTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtc                                                                                                                                                >  1:1496436/1‑150 (MQ=255)
       cTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtc                                                                                                                                                >  2:8997/1‑150 (MQ=255)
       cTCACCAGCCAGGGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtc                                                                                                                                                <  2:1517668/150‑1 (MQ=255)
        tCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcg                                                                                                                                               >  1:614506/1‑150 (MQ=255)
          aCCAGCGAGTGGTGGCTGCCGTATGACCCTCAGGCGATTGATTTGCCGTCGGGCCTGCTTTCGGCGGATGCGCAGCACTGTCTGGGCACCGAGCACTTAGGTCTCGATATTTTCTCGCGGCTGATGGCAGCGACACGCGGGTCGCtgcaa                                                                                                                                             <  2:218364/150‑5 (MQ=255)
           ccagccagTGGTGGCTGCCGTATGACCCTCAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggtt                                                                                                                                            >  1:868492/1‑150 (MQ=255)
            cagccagTGGTGGCTGCCGTATGACCCACAGTCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggttc                                                                                                                                           <  2:1363279/150‑1 (MQ=255)
               ccagTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggttcggt                                                                                                                                        <  1:789937/150‑1 (MQ=255)
                cagTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTa                                                                                                                                       <  2:1404235/150‑1 (MQ=255)
                 agTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTaa                                                                                                                                      >  1:737599/1‑150 (MQ=255)
                 agTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACGTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGCCCCGCGTGTCGCTCCGTTCGGTaa                                                                                                                                      >  1:1125350/1‑150 (MQ=255)
                   gggtggCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATg                                                                                                                                    <  2:829317/149‑1 (MQ=255)
                    ggtggCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATgg                                                                                                                                   <  2:1070691/150‑1 (MQ=255)
                         cTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGgcctgc                                                                                                                              >  1:681482/1‑150 (MQ=255)
                          tGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGgcctgcc                                                                                                                             <  2:1602483/150‑1 (MQ=255)
                                aTGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGTCCGCGACCCGCGTGTAGCACGGTACGGTCATGGCCTTCctgctgc                                                                                                                       >  2:602245/1‑150 (MQ=255)
                                aTGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCctgctgc                                                                                                                       <  1:845756/150‑1 (MQ=255)
                                aTGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCctgctgc                                                                                                                       <  2:178057/150‑1 (MQ=255)
                                 tGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCctgctgct                                                                                                                      >  2:1323773/1‑150 (MQ=255)
                                 tGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCctgctgct                                                                                                                      <  2:1457615/150‑1 (MQ=255)
                                  gACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCctgctgctg                                                                                                                     <  1:12712/150‑1 (MQ=255)
                                   aCCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCCGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTgg                                                                                                                    >  1:240102/1‑150 (MQ=255)
                                    cccACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGt                                                                                                                   >  2:45086/1‑150 (MQ=255)
                                    cccACAGGCGATTGATTTGCAGTCGCGCCTGGTTTCGCCGGATGCGCAGCACTGGCTGGGAAACGATCACTTAGGTACCGATATTTTCTAGCAGCTGATGGCAGCGACACCCGAGTCGCACGGTACGTTAATGGGATGCCTGCTGCTggg                                                                                                                   >  2:361484/1‑149 (MQ=255)
                                     ccACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTGCGGTAATGGCCTGCCTGCTGCTGGTg                                                                                                                  <  1:190263/150‑1 (MQ=255)
                                     ccACAGGCGATTGATTGGCCGGCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTTGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTg                                                                                                                  <  2:1652209/150‑1 (MQ=255)
                                      cacaGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGc                                                                                                                 >  2:793770/1‑150 (MQ=255)
                                      cacaGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGc                                                                                                                 <  1:1598016/150‑1 (MQ=255)
                                       acaGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCGGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGcg                                                                                                                >  1:999556/1‑149 (MQ=255)
                                        caGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTg                                                                                                               <  2:1087953/150‑1 (MQ=255)
                                        caGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTg                                                                                                               <  1:1338214/150‑1 (MQ=255)
                                          ggCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGAc                                                                                                             >  1:63890/1‑150 (MQ=255)
                                          ggCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGAc                                                                                                             <  2:105137/150‑1 (MQ=255)
                                          ggCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATAACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGAc                                                                                                             >  2:735364/1‑150 (MQ=255)
                                              attgattTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGGTCGGTAATGGCCTGCCTGCTGCTGGTGCTGCCagtt                                                                                                         >  1:1115931/1‑147 (MQ=255)
                                                tgattTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAgg                                                                                                       <  1:8997/150‑1 (MQ=255)
                                                 gattTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAggg                                                                                                      >  2:1393551/1‑150 (MQ=255)
                                                  attTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGc                                                                                                     <  2:1239290/150‑1 (MQ=255)
                                                    ttGCCGTCGCGCCTGCTTTCGCCGGCTGGGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTg                                                                                                   <  2:637083/150‑1 (MQ=255)
                                                     tGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTgg                                                                                                  <  2:565516/150‑1 (MQ=255)
                                                     tGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCGCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTgg                                                                                                  <  1:1122981/150‑1 (MQ=255)
                                                      gCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCAGATGGCATCGACCCGCGTGTCGCTCGGTTCGGTAAAGGCCTCCCGGCTGCTCGTGCTGAACTTAGGGcgtgt                                                                                                 >  2:1149220/1‑146 (MQ=255)
                                                       ccGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCGGGTGCTGACATTAGGGCTGGtt                                                                                                >  1:1085333/1‑150 (MQ=255)
                                                        cGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGttt                                                                                               >  2:539856/1‑149 (MQ=255)
                                                          tCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTAtt                                                                                             <  1:1278069/150‑1 (MQ=255)
                                                           cgcgCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTg                                                                                            >  2:105456/1‑150 (MQ=255)
                                                           cgcgCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCGGGTTATTg                                                                                            >  1:886722/1‑150 (MQ=255)
                                                              gcCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTggcg                                                                                         <  1:248917/150‑1 (MQ=255)
                                                              gcCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTggcg                                                                                         <  1:1683071/150‑1 (MQ=255)
                                                                cTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGCTGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTggcggc                                                                                       >  1:312753/1‑150 (MQ=255)
                                                                 tGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCa                                                                                      <  2:663768/150‑1 (MQ=255)
                                                                   cTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAgc                                                                                    >  2:1482717/1‑150 (MQ=255)
                                                                      tCGCCGGCTGCGCAGCACTGGCGGGGCACCTATCACTTCGGGCTCGATATTTTCTCGCGGCTGATGTCAGCGACACGCGTGTAGCTCGGTTCGTTTATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTAATGGCGGCAGCGcc                                                                                 <  1:1142810/150‑1 (MQ=255)
                                                                       cGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCCGGTTATTGGCGGCAGCGCCg                                                                                <  1:931539/150‑1 (MQ=255)
                                                                        gCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGCCCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCGGCTGCTGGTGCTGACAGTAGGGCTGGTTATTGGCGGCCGCGCCgg                                                                               >  1:829854/1‑150 (MQ=255)
                                                                        gCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACAATAGGGCTGGTTATTGGCGGCAGCGCCgg                                                                               >  1:51082/1‑150 (MQ=255)
                                                                          cGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCGCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGt                                                                             <  2:1007619/150‑1 (MQ=255)
                                                                                cgcAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTg                                                                       >  2:533847/1‑150 (MQ=255)
                                                                                 gcagcaCTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGTCCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCGGGTTATTGGCGGCCGCGCCGGGTTGATTgg                                                                      >  2:1826823/1‑150 (MQ=255)
                                                                                 gcagcaCTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGACGGGTTGATTgg                                                                      >  2:396166/1‑150 (MQ=255)
                                                                                        tggctggGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGgcgc                                                               >  1:1006541/1‑150 (MQ=255)
                                                                                        tggctggGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGgcgc                                                               >  1:1000003/1‑150 (MQ=255)
                                                                                         ggctggGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGcgcg                                                              >  2:595494/1‑150 (MQ=255)
                                                                                            ggggCACCGATCACTTAGGTCGCGATATTTTCTCTCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGGTCGGTAATGGCCTGCCGGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTg                                                           <  2:995042/149‑1 (MQ=255)
                                                                                               gCACCGATCACTTCGGGCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATc                                                        <  1:1154499/150‑1 (MQ=255)
                                                                                               gCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCTGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATc                                                        <  2:586526/150‑1 (MQ=255)
                                                                                               gCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTGATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATc                                                        <  1:378281/150‑1 (MQ=255)
                                                                                                cACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCa                                                       >  1:932411/1‑150 (MQ=255)
                                                                                                    gATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGcc                                                   >  2:730662/1‑150 (MQ=255)
                                                                                                     aTCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGGCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGcca                                                  <  2:1192767/150‑1 (MQ=255)
                                                                                                             tgTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCTCCATgcgc                                          >  1:139642/2‑150 (MQ=255)
                                                                                                              gTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGTTTATTGGCGGCAGCGCCGGGTGGATTGGCGGGCGCGTTGATCAGGCCACCATGcgcg                                         >  1:585656/1‑150 (MQ=255)
                                                                                                              gTCGCGATATTTTCTCGCGGCTGAGGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGcgcg                                         <  2:228615/150‑1 (MQ=255)
                                                                                                                 gcgATTATTTCTCGCGGCTGATGGCAGCGACCCGCGTGGCGCTCGGATCGGTAATGGACTGCCTGCGGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCg                                      <  1:1149220/150‑1 (MQ=255)
                                                                                                                 gcgATATTTTCTCGCGGCTGATCGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGACTGATGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGACGGGTTGATTGCCGGGAGCGTTGATCAGGACACCATGCGCGTCg                                      >  2:1203878/1‑150 (MQ=255)
                                                                                                                  cgATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGc                                     >  1:1067964/1‑150 (MQ=255)
                                                                                                                         ttCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGCTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATg                              <  1:735364/150‑1 (MQ=255)
                                                                                                                           ctcGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGtt                            <  2:682593/150‑1 (MQ=255)
                                                                                                                           ctcGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGAGCGTTGATCAGGCCACCATGCGCGTCGCCGCTATggt                            >  2:306984/1‑148 (MQ=255)
                                                                                                                             cgcgGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTGTTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTa                          <  1:1617482/150‑1 (MQ=255)
                                                                                                                             cgcgGCTGATGGCAGCGACCCGCGGGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTa                          <  2:1093852/150‑1 (MQ=255)
                                                                                                                               cgGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATg                        <  1:380981/150‑1 (MQ=255)
                                                                                                                               cgGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATg                        <  2:1445791/150‑1 (MQ=255)
                                                                                                                                ggCTGATGGCAGCGACCCGCGTGTCGGTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATAAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCAGATATGGTTATGa                       >  2:1269490/1‑150 (MQ=255)
                                                                                                                                 gCTGATGGCAGCGACCCGCGTGGCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGGGCTGACATTAGGGCTGGGTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGAc                      <  1:1246751/150‑1 (MQ=255)
                                                                                                                                        gCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGGTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGGCGCCGAGATGTTTATGACCTTCCCg               >  2:703687/1‑150 (MQ=255)
                                                                                                                                        gCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCg               >  1:157414/1‑150 (MQ=255)
                                                                                                                                         gaGCGACACGCGGGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGa              >  1:1661782/2‑150 (MQ=255)
                                                                                                                                         cAGCGACCCGGGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGGTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCGGa              >  1:191595/1‑150 (MQ=255)
                                                                                                                                         cAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCgc              >  2:1238787/1‑149 (MQ=255)
                                                                                                                                         cAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGa              >  1:1732276/1‑150 (MQ=255)
                                                                                                                                         cAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGa              <  2:441187/150‑1 (MQ=255)
                                                                                                                                                  gcgTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTc     <  1:1324031/150‑1 (MQ=255)
                                                                                                                                                     tgtCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGGTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGt  <  1:45086/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGT  >  NC_000913/3616250‑3616548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑