Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,648,128 G→T 5.0% noncoding (66/84 nt) agrA → inactive antisense sRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,648,1280GT 5.0% 278.4 / 5.3 100noncoding (66/84 nt)agrAinactive antisense sRNA
Reads supporting (aligned to +/- strand):  ref base G (37/58);  new base T (3/2);  total (40/60)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-01

Only 100 of 101 total aligned reads displayed.

AACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGC  >  NC_000913/3647980‑3648277
                                                                                                                                                     |                                                                                                                                                     
aaCTGGATAATCATACAGTACATGCATGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGc                                                                                                                                                      <  1:451825/150‑1 (MQ=255)
aaCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGc                                                                                                                                                      >  2:570976/1‑150 (MQ=255)
  atGGATAATCATACAGTACATGCTGGTTATAAAACCAGCACGTACTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCaa                                                                                                                                                    <  1:49363/149‑1 (MQ=255)
     gATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGt                                                                                                                                                 >  1:1697679/1‑150 (MQ=255)
         aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGCTGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGt                                                                                                                                             >  2:251783/1‑150 (MQ=255)
           aaTACTGTAAATGCAGGTTATAAAACCAGCACGTCCTTGCAATTGTTTCAGTATGGTATTAGAATTGATGCGTTAGATTATGTCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttt                                                                                                                                           <  2:143412/149‑1 (MQ=37)
             tACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttt                                                                                                                                         <  1:888473/150‑1 (MQ=255)
              aCAGTACATGCAGGTTATAAAACCAGCACTTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                        <  1:310082/150‑1 (MQ=255)
              aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                        >  2:1251130/1‑150 (MQ=255)
               cAGTACATGCAGGTTATAACACCTGCTCGTCCTTGCAATAGGTTCAGTATGGTATTAGCATTGATGCGTTATATGAGGGCTATCTCACTCCAGTCAGACCCACCAACTCAGGGCTGGAAAGT‑AAAATACCGTCGCAAAGTCGGttttttt                                                                                                                                       <  2:276670/150‑1 (MQ=25)
               cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                       <  1:1211584/150‑1 (MQ=255)
               cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                       <  2:1607669/150‑1 (MQ=255)
               cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTAAGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                       <  2:569126/150‑1 (MQ=255)
               cAGTACATGCAGGTTATAAAACAAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCTTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAACCCGACGCAAAGTCGGttttttt                                                                                                                                       <  1:852103/150‑1 (MQ=255)
               aaGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAACCGACTTTGCGTCGGtttttt                                                                                                                                        <  2:862042/149‑1 (MQ=38)
                aGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACTAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTa                                                                                                                                      >  1:1341082/1‑150 (MQ=255)
                 gTACATGCAGTTTATAAAACCAGCAAGTACTTTCAATAGTTTCAGTATTGTATTAGCATAGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGATCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                     <  2:69688/150‑1 (MQ=25)
                  tACATGCAGGTTATAAAACCAGCACGTCCTTGCTATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACTTTGCGTCGGttttttactt                                                                                                                                    <  1:1282401/150‑5 (MQ=37)
                  tACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACa                                                                                                                                    <  1:1606891/150‑1 (MQ=255)
                    cgtGCTGGTTTTATAACCTGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGACTGGAAAGT‑AAAAACCCGAAGCAAAATAGGTTTTATTACAta                                                                                                                                  >  2:808221/3‑149 (MQ=255)
                    cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAGCTCGGGGGTGGAAAGT‑TAAAAACAGCAGAAAAGTCGGGATTTTTACagc                                                                                                                                  >  1:1026498/1‑148 (MQ=255)
                    cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTATCAGTATGGTATTAGCATAGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCAACAAACTCAGGGCTGGAAAGT‑AAAAAACCGCCGCATAGTCGGTTTTTTTACATc                                                                                                                                  >  1:1196791/1‑150 (MQ=37)
                      tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCg                                                                                                                                >  1:1465186/1‑150 (MQ=255)
                      tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCg                                                                                                                                >  1:767075/1‑150 (MQ=255)
                      tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTAAGAGCCACAAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATcag                                                                                                                                >  1:727239/1‑148 (MQ=255)
                          ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAtt                                                                                                                            >  2:1211149/1‑150 (MQ=255)
                            ttATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCg                                                                                                                          <  1:64004/150‑1 (MQ=255)
                            ttATAAAACCAGCACGTCCTTGCAATACTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCACGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCg                                                                                                                          <  2:1370863/150‑1 (MQ=255)
                             tataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTctg                                                                                                                         >  1:1407043/1‑148 (MQ=255)
                              ataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATATCACTCCAGTCAGAGACACCAACTCAGGGCTGGAAAGT‑AAAAAAACGACGCAAACTCGGTTTTTTTACATACTGATTccgc                                                                                                                        >  2:520007/1‑147 (MQ=37)
                                aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                      >  2:1135494/1‑150 (MQ=255)
                                aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                      >  2:760353/1‑150 (MQ=255)
                                aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                      >  2:610240/1‑150 (MQ=255)
                                  aaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAg                                                                                                                    <  1:697159/150‑1 (MQ=255)
                                    ccAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGc                                                                                                                  <  2:1635491/150‑1 (MQ=255)
                                    ccAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGACAGCAAAGT‑AAAAAACAGAAGCGAATTAGGGTATTTTACATCAGGAATCGGACAAGGc                                                                                                                  >  2:1514595/1‑150 (MQ=255)
                                          cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                            >  2:839766/1‑150 (MQ=255)
                                          cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                            <  2:1811707/150‑1 (MQ=255)
                                               ttGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACg                                                                                                       >  1:1264110/1‑150 (MQ=255)
                                               ttGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACg                                                                                                       <  2:221012/150‑1 (MQ=255)
                                                  cAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATg                                                                                                    <  2:677708/150‑1 (MQ=255)
                                                      aGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGAccc                                                                                                <  2:1780805/150‑1 (MQ=255)
                                                              tATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAg                                                                                        >  1:1316210/1‑150 (MQ=255)
                                                              tATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAg                                                                                        <  1:615022/150‑1 (MQ=255)
                                                                    aTTACCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGTGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatata                                                                                  <  2:148161/150‑1 (MQ=255)
                                                                        gCATTGATGCGTTAGATGATTGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATc                                                                              <  1:774355/150‑1 (MQ=255)
                                                                         cATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCg                                                                             <  2:942839/150‑1 (MQ=255)
                                                                           ttGATGCGTTTGATGCTGGCTAGCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTc                                                                           <  2:1603263/150‑1 (MQ=255)
                                                                               tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTg                                                                       <  1:820571/150‑1 (MQ=255)
                                                                               tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTg                                                                       <  1:570976/150‑1 (MQ=255)
                                                                                  gTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGa                                                                    <  1:610240/150‑1 (MQ=255)
                                                                                          atgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATTTAAATCGTCACTGCGATATATAcc                                                            <  2:1456700/150‑1 (MQ=255)
                                                                                           tgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCg                                                           >  2:1770981/1‑150 (MQ=255)
                                                                                                aTCTCACTCCAGTCATATCCACCAACTCATGGCTTGAAAGT‑AAAAAACCGACGCTAAGTCGGTTTTTTTTCATCCGGATTCGGACAAGGCTTAATATGATGATGACCCAGTGAATGTATATAAATCGTCACTGCGATATATACCGAAGTg                                                      <  2:284137/150‑1 (MQ=37)
                                                                                                  ctcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGG‑TTTTCTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                   <  2:1697679/150‑1 (MQ=255)
                                                                                                   tctcTCCAGTCAGAGCCACCCACTCACGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATACGGCCAAGCCTTAATATGACGATGACCCAGTGAAACTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                   <  2:1196791/147‑1 (MQ=37)
                                                                                                   tcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                   <  1:219305/150‑1 (MQ=255)
                                                                                                   tcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                   <  1:1178493/150‑1 (MQ=255)
                                                                                                        ccAGTCAGATACACCAACTCAGGGATGGAAAGT‑AAAAAACCGACGCTAAGTCTGTTTTTTTACATCCTGATTCGCACAAGGCTTAATATGACGATGACCCAGTGAAAGTATAGAAATCGTCACTGCGATATATACCGAAGTGctccctcc                                              <  1:281395/150‑1 (MQ=37)
                                                                                                         cAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCg                                             <  2:460190/150‑1 (MQ=255)
                                                                                                           gTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGcc                                           <  1:839766/150‑1 (MQ=255)
                                                                                                           ctCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGcc                                           <  2:478595/149‑1 (MQ=255)
                                                                                                            tCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCa                                          <  2:1008221/150‑1 (MQ=255)
                                                                                                              agagCCACCAACTCAGGGCAGGAAAGT‑AAAAAACCGACGCAAAGGCGGTTTTTTTACATCCGGATTCGGAAAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGc                                        <  2:702076/150‑1 (MQ=255)
                                                                                                                 gCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTga                                     >  2:322153/1‑150 (MQ=255)
                                                                                                                   caccaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                   <  2:1741390/150‑1 (MQ=255)
                                                                                                                    accaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaaga                                  <  1:405075/150‑1 (MQ=255)
                                                                                                                         cTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCg                             <  1:1218678/150‑1 (MQ=255)
                                                                                                                          tCAGGGCTGGAAAGT‑AAAAAACCGACGCAAATTCGGTTTTTTTACTTCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGc                            <  2:1630219/150‑1 (MQ=255)
                                                                                                                          tCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGc                            <  2:1341082/150‑1 (MQ=255)
                                                                                                                           cAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCt                           <  1:1502854/150‑1 (MQ=255)
                                                                                                                           cAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCt                           <  2:577012/150‑1 (MQ=255)
                                                                                                                            aGGGCTGGAAAGT‑AAAAAAACGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGATATCGCTa                          <  1:1695056/150‑1 (MQ=255)
                                                                                                                               gCTGGAAAGT‑AAAAAACCGACGCAATGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                       <  2:721221/150‑1 (MQ=255)
                                                                                                                               gCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                       <  1:1135494/150‑1 (MQ=255)
                                                                                                                               gCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                       >  2:932839/1‑150 (MQ=255)
                                                                                                                               gCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACAGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                       >  2:1377074/1‑150 (MQ=255)
                                                                                                                                cTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtta                      >  2:803206/1‑149 (MQ=255)
                                                                                                                                cTGGAAAGA‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTc                      <  1:878508/150‑1 (MQ=255)
                                                                                                                                  ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                    >  1:1654531/1‑150 (MQ=255)
                                                                                                                                  ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACAGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                    >  2:721461/1‑150 (MQ=255)
                                                                                                                                   gAACGT‑AAAAAACCGAAGCAAAGTAGCTTTTTTTACATCACGATTAGGACAAGGCTTAATATGACGATGACCCACGGAAAGTATATAAATCTTCACTGCTAAATATAACGAAGTGCTCCCTCCTCCAGCTGAAGCAATCGCTAATTCGTg                   >  2:106715/1‑150 (MQ=255)
                                                                                                                                     aaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCa                 <  2:546295/150‑1 (MQ=255)
                                                                                                                                      aGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCaa                >  2:46502/1‑150 (MQ=255)
                                                                                                                                       gtaaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGCCAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATAGTCACTGCGATATATACCGAAGTGCTCACTCCGCCAGCTGAAGAAATCGCTAATTCTTGCaa                >  2:1488651/4‑150 (MQ=255)
                                                                                                                                       gT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt               >  2:1805145/1‑150 (MQ=255)
                                                                                                                                       gT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt               <  1:760353/150‑1 (MQ=255)
                                                                                                                                        taaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt               >  2:1296631/3‑150 (MQ=255)
                                                                                                                                        t‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGTCGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATg              <  2:1211893/150‑1 (MQ=255)
                                                                                                                                          aaaaaaCCGACTTTGCGTCGGTTTTTTTACATCATGATTCGGACAAGGCTAAATATGACGATGACCCAGTGAAAGTATATAAATCGTAACAGCGATATATACCGAAGTTCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGAAATGt             >  1:538630/1‑150 (MQ=255)
                                                                                                                                           aaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGtt            >  2:581845/1‑150 (MQ=255)
                                                                                                                                           aaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGtt            <  2:1142091/150‑1 (MQ=255)
                                                                                                                                              aaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCTTCACTGCGATATATAACGAAGTGCTCCCTCCGCCAGCTGAAGAACTCGCTAATTCTTGCAATGTTAGc         >  1:526699/1‑150 (MQ=255)
                                                                                                                                               aCCGCCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATAAACCGAAGTGCTCCCTCAGCCAGCTGAAGAAATCGCTAATTCTTGCCATGTTAGcc        >  1:475509/1‑150 (MQ=255)
                                                                                                                                                ccGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCa       >  1:1431300/1‑150 (MQ=255)
                                                                                                                                                   aCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTg    <  1:791545/150‑1 (MQ=255)
                                                                                                                                                    cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg   <  1:1410448/150‑1 (MQ=255)
                                                                                                                                                    cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg   >  2:1583091/1‑150 (MQ=255)
                                                                                                                                                    cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg   >  2:1594765/1‑150 (MQ=255)
                                                                                                                                                     gCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGc  <  1:251783/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGC  >  NC_000913/3647980‑3648277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑