Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,648,130 A→T 6.4% noncoding (68/84 nt) agrA → inactive antisense sRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,648,1300AT 6.4% 292.4 / 5.2 102noncoding (68/84 nt)agrAinactive antisense sRNA
Reads supporting (aligned to +/- strand):  ref base A (37/57);  new base T (3/4);  total (41/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTA  >  NC_000913/3647982‑3648279
                                                                                                                                                     |                                                                                                                                                     
atGGATAATCATACAGTACATGCTGGTTATAAAACCAGCACGTACTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCaa                                                                                                                                                      <  1:49363/149‑1 (MQ=255)
   gATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGt                                                                                                                                                   >  1:1697679/1‑150 (MQ=255)
       aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGCTGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGt                                                                                                                                               >  2:251783/1‑150 (MQ=255)
         aaTACTGTAAATGCAGGTTATAAAACCAGCACGTCCTTGCAATTGTTTCAGTATGGTATTAGAATTGATGCGTTAGATTATGTCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttt                                                                                                                                             <  2:143412/149‑1 (MQ=37)
           tACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttt                                                                                                                                           <  1:888473/150‑1 (MQ=255)
            aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                          >  2:1251130/1‑150 (MQ=255)
             cAGTACATGCAGGTTATAACACCTGCTCGTCCTTGCAATAGGTTCAGTATGGTATTAGCATTGATGCGTTATATGAGGGCTATCTCACTCCAGTCAGACCCACCAACTCAGGGCTGGAAAGT‑AAAATACCGTCGCAAAGTCGGttttttt                                                                                                                                         <  2:276670/150‑1 (MQ=25)
             cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                         <  1:1211584/150‑1 (MQ=255)
             cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                         <  2:1607669/150‑1 (MQ=255)
             cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTAAGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                         <  2:569126/150‑1 (MQ=255)
             cAGTACATGCAGGTTATAAAACAAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCTTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAACCCGACGCAAAGTCGGttttttt                                                                                                                                         <  1:852103/150‑1 (MQ=255)
             aaGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAACCGACTTTGCGTCGGtttttt                                                                                                                                          <  2:862042/149‑1 (MQ=38)
              aGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACTAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTa                                                                                                                                        >  1:1341082/1‑150 (MQ=255)
               gTACATGCAGTTTATAAAACCAGCAAGTACTTTCAATAGTTTCAGTATTGTATTAGCATAGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGATCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                       <  2:69688/150‑1 (MQ=25)
                tACATGCAGGTTATAAAACCAGCACGTCCTTGCTATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACTTTGCGTCGGttttttactt                                                                                                                                      <  1:1282401/150‑5 (MQ=37)
                tACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACa                                                                                                                                      <  1:1606891/150‑1 (MQ=255)
                  cgtGCTGGTTTTATAACCTGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGACTGGAAAGT‑AAAAACCCGAAGCAAAATAGGTTTTATTACAta                                                                                                                                    >  2:808221/3‑149 (MQ=255)
                  cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAGCTCGGGGGTGGAAAGT‑TAAAAACAGCAGAAAAGTCGGGATTTTTACagc                                                                                                                                    >  1:1026498/1‑148 (MQ=255)
                  cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTATCAGTATGGTATTAGCATAGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCAACAAACTCAGGGCTGGAAAGT‑AAAAAACCGCCGCATAGTCGGTTTTTTTACATc                                                                                                                                    >  1:1196791/1‑150 (MQ=37)
                    tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCg                                                                                                                                  >  1:1465186/1‑150 (MQ=255)
                    tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCg                                                                                                                                  >  1:767075/1‑150 (MQ=255)
                    tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTAAGAGCCACAAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATcag                                                                                                                                  >  1:727239/1‑148 (MQ=255)
                        ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAtt                                                                                                                              >  2:1211149/1‑150 (MQ=255)
                          ttATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCg                                                                                                                            <  1:64004/150‑1 (MQ=255)
                          ttATAAAACCAGCACGTCCTTGCAATACTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCACGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCg                                                                                                                            <  2:1370863/150‑1 (MQ=255)
                           tataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTctg                                                                                                                           >  1:1407043/1‑148 (MQ=255)
                            ataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATATCACTCCAGTCAGAGACACCAACTCAGGGCTGGAAAGT‑AAAAAAACGACGCAAACTCGGTTTTTTTACATACTGATTccgc                                                                                                                          >  2:520007/1‑147 (MQ=37)
                              aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                        >  2:1135494/1‑150 (MQ=255)
                              aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                        >  2:610240/1‑150 (MQ=255)
                              aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                        >  2:760353/1‑150 (MQ=255)
                                aaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAg                                                                                                                      <  1:697159/150‑1 (MQ=255)
                                  ccAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGc                                                                                                                    <  2:1635491/150‑1 (MQ=255)
                                  ccAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGACAGCAAAGT‑AAAAAACAGAAGCGAATTAGGGTATTTTACATCAGGAATCGGACAAGGc                                                                                                                    >  2:1514595/1‑150 (MQ=255)
                                        cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                              >  2:839766/1‑150 (MQ=255)
                                        cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                              <  2:1811707/150‑1 (MQ=255)
                                             ttGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACg                                                                                                         >  1:1264110/1‑150 (MQ=255)
                                                cAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATg                                                                                                      <  2:677708/150‑1 (MQ=255)
                                                    aGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGAccc                                                                                                  <  2:1780805/150‑1 (MQ=255)
                                                            tATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAg                                                                                          <  1:615022/150‑1 (MQ=255)
                                                            tATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAg                                                                                          >  1:1316210/1‑150 (MQ=255)
                                                                  aTTACCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGTGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatata                                                                                    <  2:148161/150‑1 (MQ=255)
                                                                      gCATTGATGCGTTAGATGATTGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATc                                                                                <  1:774355/150‑1 (MQ=255)
                                                                       cATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCg                                                                               <  2:942839/150‑1 (MQ=255)
                                                                         ttGATGCGTTTGATGCTGGCTAGCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTc                                                                             <  2:1603263/150‑1 (MQ=255)
                                                                             tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTg                                                                         <  1:820571/150‑1 (MQ=255)
                                                                             tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTg                                                                         <  1:570976/150‑1 (MQ=255)
                                                                                gTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGa                                                                      <  1:610240/150‑1 (MQ=255)
                                                                                        atgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATTTAAATCGTCACTGCGATATATAcc                                                              <  2:1456700/150‑1 (MQ=255)
                                                                                         tgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCg                                                             >  2:1770981/1‑150 (MQ=255)
                                                                                              aTCTCACTCCAGTCATATCCACCAACTCATGGCTTGAAAGT‑AAAAAACCGACGCTAAGTCGGTTTTTTTTCATCCGGATTCGGACAAGGCTTAATATGATGATGACCCAGTGAATGTATATAAATCGTCACTGCGATATATACCGAAGTg                                                        <  2:284137/150‑1 (MQ=37)
                                                                                                ctcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGG‑TTTTCTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                     <  2:1697679/150‑1 (MQ=255)
                                                                                                 tcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                     <  1:1178493/150‑1 (MQ=255)
                                                                                                 tcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctc                                                     <  1:219305/150‑1 (MQ=255)
                                                                                                      ccAGTCAGATACACCAACTCAGGGATGGAAAGT‑AAAAAACCGACGCTAAGTCTGTTTTTTTACATCCTGATTCGCACAAGGCTTAATATGACGATGACCCAGTGAAAGTATAGAAATCGTCACTGCGATATATACCGAAGTGctccctcc                                                <  1:281395/150‑1 (MQ=37)
                                                                                                       cAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCg                                               <  2:460190/150‑1 (MQ=255)
                                                                                                         gTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGcc                                             <  1:839766/150‑1 (MQ=255)
                                                                                                         ctCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGcc                                             <  2:478595/149‑1 (MQ=255)
                                                                                                          tCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCa                                            <  2:1008221/150‑1 (MQ=255)
                                                                                                            agagCCACCAACTCAGGGCAGGAAAGT‑AAAAAACCGACGCAAAGGCGGTTTTTTTACATCCGGATTCGGAAAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGc                                          <  2:702076/150‑1 (MQ=255)
                                                                                                               gCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTga                                       >  2:322153/1‑150 (MQ=255)
                                                                                                                 caccaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                     <  2:1741390/150‑1 (MQ=255)
                                                                                                                  accaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaaga                                    <  1:405075/150‑1 (MQ=255)
                                                                                                                       cTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCg                               <  1:1218678/150‑1 (MQ=255)
                                                                                                                        tCAGGGCTGGAAAGT‑AAAAAACCGACGCAAATTCGGTTTTTTTACTTCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGc                              <  2:1630219/150‑1 (MQ=255)
                                                                                                                        tCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGc                              <  2:1341082/150‑1 (MQ=255)
                                                                                                                         cAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCt                             <  2:577012/150‑1 (MQ=255)
                                                                                                                         cAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCt                             <  1:1502854/150‑1 (MQ=255)
                                                                                                                          aGGGCTGGAAAGT‑AAAAAAACGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGATATCGCTa                            <  1:1695056/150‑1 (MQ=255)
                                                                                                                             gCTGGAAAGT‑AAAAAACCGACGCAATGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                         <  2:721221/150‑1 (MQ=255)
                                                                                                                             gCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                         <  1:1135494/150‑1 (MQ=255)
                                                                                                                             gCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                         >  2:932839/1‑150 (MQ=255)
                                                                                                                             gCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACAGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtt                         >  2:1377074/1‑150 (MQ=255)
                                                                                                                              cTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAAtta                        >  2:803206/1‑149 (MQ=255)
                                                                                                                              cTGGAAAGA‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTc                        <  1:878508/150‑1 (MQ=255)
                                                                                                                                ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                      >  1:1654531/1‑150 (MQ=255)
                                                                                                                                ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACAGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                      >  2:721461/1‑150 (MQ=255)
                                                                                                                                 gAACGT‑AAAAAACCGAAGCAAAGTAGCTTTTTTTACATCACGATTAGGACAAGGCTTAATATGACGATGACCCACGGAAAGTATATAAATCTTCACTGCTAAATATAACGAAGTGCTCCCTCCTCCAGCTGAAGCAATCGCTAATTCGTg                     >  2:106715/1‑150 (MQ=255)
                                                                                                                                   aaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCa                   <  2:546295/150‑1 (MQ=255)
                                                                                                                                    aGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCaa                  >  2:46502/1‑150 (MQ=255)
                                                                                                                                     gtaaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGCCAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATAGTCACTGCGATATATACCGAAGTGCTCACTCCGCCAGCTGAAGAAATCGCTAATTCTTGCaa                  >  2:1488651/4‑150 (MQ=255)
                                                                                                                                     gT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt                 >  2:1805145/1‑150 (MQ=255)
                                                                                                                                     gT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt                 <  1:760353/150‑1 (MQ=255)
                                                                                                                                      taaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt                 >  2:1296631/3‑150 (MQ=255)
                                                                                                                                      t‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGTCGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATg                <  2:1211893/150‑1 (MQ=255)
                                                                                                                                        aaaaaaCCGACTTTGCGTCGGTTTTTTTACATCATGATTCGGACAAGGCTAAATATGACGATGACCCAGTGAAAGTATATAAATCGTAACAGCGATATATACCGAAGTTCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGAAATGt               >  1:538630/1‑150 (MQ=255)
                                                                                                                                         aaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGtt              <  2:1142091/150‑1 (MQ=255)
                                                                                                                                         aaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGtt              >  2:581845/1‑150 (MQ=255)
                                                                                                                                            aaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCTTCACTGCGATATATAACGAAGTGCTCCCTCCGCCAGCTGAAGAACTCGCTAATTCTTGCAATGTTAGc           >  1:526699/1‑150 (MQ=255)
                                                                                                                                             aCCGCCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATAAACCGAAGTGCTCCCTCAGCCAGCTGAAGAAATCGCTAATTCTTGCCATGTTAGcc          >  1:475509/1‑150 (MQ=255)
                                                                                                                                              ccGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCa         >  1:1431300/1‑150 (MQ=255)
                                                                                                                                                 aCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTg      <  1:791545/150‑1 (MQ=255)
                                                                                                                                                  cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg     >  2:1594765/1‑150 (MQ=255)
                                                                                                                                                  cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg     <  1:1410448/150‑1 (MQ=255)
                                                                                                                                                  cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg     >  2:1583091/1‑150 (MQ=255)
                                                                                                                                                   gCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGc    <  1:251783/150‑1 (MQ=255)
                                                                                                                                                    cAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt   <  1:663701/150‑1 (MQ=255)
                                                                                                                                                    cAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt   >  1:1634478/1‑150 (MQ=255)
                                                                                                                                                    cAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt   <  2:1264110/150‑1 (MQ=255)
                                                                                                                                                    cAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt   >  1:1707906/1‑150 (MQ=255)
                                                                                                                                                     aaaGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTa  <  1:1251130/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTA  >  NC_000913/3647982‑3648279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑