Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,657,599 T→A 7.4% intergenic (+32/+387) hdeD → / ← arrS acid‑resistance membrane protein/antisense sRNA ArrS, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,657,5990TA 7.4% 380.3 / 13.8 133intergenic (+32/+387)hdeD/arrSacid‑resistance membrane protein/antisense sRNA ArrS, function unknown
Reads supporting (aligned to +/- strand):  ref base T (64/59);  new base A (5/5);  total (69/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 134 total aligned reads displayed.

CCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCA  >  NC_000913/3657450‑3657746
                                                                                                                                                     |                                                                                                                                                   
ccTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACt                                                                                                                                                     >  1:263006/1‑150 (MQ=255)
     gTGCAACGCCTATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCAGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGGTAAGCAGCAATAATTAACCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                                                                                <  1:1779530/150‑1 (MQ=255)
     gTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                                                                                >  2:871282/1‑150 (MQ=255)
     gTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                                                                                <  1:1253097/150‑1 (MQ=255)
     gTGCAAAGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                                                                                <  2:335667/150‑1 (MQ=255)
       gCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtgtg                                                                                                                                              >  1:658318/1‑150 (MQ=255)
       gCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtgtg                                                                                                                                              <  1:572125/150‑1 (MQ=255)
        cAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGa                                                                                                                                             >  1:672969/1‑150 (MQ=255)
         atcGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGAc                                                                                                                                            <  1:1158258/148‑1 (MQ=255)
         aaCGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGAc                                                                                                                                            <  1:1774828/150‑1 (MQ=255)
         aaCGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGAc                                                                                                                                            >  2:1280237/1‑150 (MQ=255)
         aaCGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGAc                                                                                                                                            <  2:602623/150‑1 (MQ=255)
          aCGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACt                                                                                                                                           <  2:52317/150‑1 (MQ=255)
          aCGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACt                                                                                                                                           >  1:317599/1‑150 (MQ=255)
           cGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTa                                                                                                                                          >  1:1650114/1‑150 (MQ=255)
           cGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTa                                                                                                                                          >  2:1269709/1‑150 (MQ=255)
             ccGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATg                                                                                                                                        >  2:1352459/1‑150 (MQ=255)
             ccGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATg                                                                                                                                        <  1:1732606/150‑1 (MQ=255)
             ccGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATg                                                                                                                                        <  2:1777484/150‑1 (MQ=255)
             ccGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATg                                                                                                                                        <  1:1269709/150‑1 (MQ=255)
               gATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGAt                                                                                                                                      <  1:1195283/150‑1 (MQ=255)
               gATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAGCATAGTCACATCAAGTGACTATGAt                                                                                                                                      <  1:1082749/150‑1 (MQ=255)
                 tGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATcc                                                                                                                                    <  2:203787/150‑1 (MQ=255)
                 tGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCATCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCAGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATcc                                                                                                                                    <  1:1116758/150‑1 (MQ=255)
                     gtCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGAtt                                                                                                                                >  2:797324/1‑150 (MQ=255)
                        ggTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTGCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAAt                                                                                                                             <  1:864529/149‑1 (MQ=255)
                          tAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATAc                                                                                                                           >  1:557851/1‑150 (MQ=255)
                             cGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActct                                                                                                                        >  2:1736538/1‑150 (MQ=255)
                                tGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACtctctc                                                                                                                     >  1:1507966/1‑150 (MQ=255)
                                      ccACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTAc                                                                                                               >  2:1563706/1‑150 (MQ=255)
                                      ccACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTAc                                                                                                               >  2:665156/1‑150 (MQ=255)
                                      ccACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTATTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTAc                                                                                                               >  1:1326325/1‑150 (MQ=255)
                                       cacaCTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACg                                                                                                              >  2:728729/1‑150 (MQ=255)
                                       cacaCTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCAACg                                                                                                              >  1:253763/1‑150 (MQ=255)
                                        acacTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCTCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGCTCATAGTCACTTGATGTGACTATGATCCGAATAATACTCACCCCGCTACGc                                                                                                             >  2:317249/1‑150 (MQ=255)
                                             ggtggGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAgtgt                                                                                                        >  2:321580/1‑150 (MQ=255)
                                                gggAATTTACCTGACAATTAGCGCCCCCAGCCTGTTCAGCTACGCCAGCTAGTTCGTTAATCAGCAATAATTACCCCGGTTGTCACCCGGAGCATAGGCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                                                                     <  1:286965/150‑1 (MQ=255)
                                                 ggAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTa                                                                                                    >  2:1266669/1‑150 (MQ=255)
                                                  gAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTat                                                                                                   >  2:260473/1‑149 (MQ=255)
                                                  gAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAg                                                                                                   >  2:602792/1‑150 (MQ=255)
                                                      tGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCa                                                                                               <  2:1809267/150‑1 (MQ=255)
                                                       gAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCaa                                                                                              >  2:1473582/1‑150 (MQ=255)
                                                          cTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGAAGTGTTGTAGATCAATTg                                                                                           >  2:1363275/1‑150 (MQ=255)
                                                               atTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCAc                                                                                      >  2:801917/1‑150 (MQ=255)
                                                                tttAGCGCCGCCAGCCTGTTCAGCTTCGCCAGGTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCCCCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATTCTCTCTCCGCTACGCAGTGTTGTAGAGCAATTGCGCACt                                                                                     >  1:266082/1‑150 (MQ=255)
                                                                     cgccgcCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCAt                                                                                >  2:568897/1‑150 (MQ=255)
                                                                      gccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCAtt                                                                               >  2:1241732/1‑150 (MQ=255)
                                                                       ccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTg                                                                              <  1:1784554/150‑1 (MQ=255)
                                                                       ccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTg                                                                              >  1:235844/1‑150 (MQ=255)
                                                                         gccAGCCTGTTCAGGTTCTGCAGTTTGTTCGTTAAGCAGCAATAATTACGCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTGTCCGCTACGCAGTTTTGTAGATCAATTGCGCACTATCTTTGaa                                                                            <  1:1439114/150‑1 (MQ=255)
                                                                         gccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGaa                                                                            <  1:1708699/150‑1 (MQ=255)
                                                                            aGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAaata                                                                         >  1:790704/1‑150 (MQ=255)
                                                                                tGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTa                                                                     >  2:1098198/1‑150 (MQ=255)
                                                                                 gTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAc                                                                    >  1:865734/1‑150 (MQ=255)
                                                                                     aGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCCCTATCATTGAAATAATTACCTGc                                                                >  2:746720/1‑150 (MQ=255)
                                                                                      gCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCt                                                               <  2:1048700/150‑1 (MQ=255)
                                                                                      gCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCt                                                               <  2:317599/150‑1 (MQ=255)
                                                                                            ccAGTTTGTTCGTTAAGCAGCAATAATTACCCGGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGat                                                         <  2:1310445/150‑1 (MQ=255)
                                                                                               gTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGattat                                                      <  1:962982/150‑1 (MQ=255)
                                                                                                tttGTTAGTTAAGCAGCAATAATTACCCCGGTTTTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGattatt                                                     <  1:1288952/150‑1 (MQ=255)
                                                                                                  tGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTc                                                   <  1:1199264/150‑1 (MQ=255)
                                                                                                   gTTCGTTAAGCAGGAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCa                                                  <  1:1441233/150‑1 (MQ=255)
                                                                                                   gTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCa                                                  <  2:641170/150‑1 (MQ=255)
                                                                                                      cGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAcc                                               <  2:81826/150‑1 (MQ=255)
                                                                                                      cGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAcc                                               >  1:644821/1‑150 (MQ=255)
                                                                                                      cGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAcc                                               >  1:532625/1‑150 (MQ=255)
                                                                                                       gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCt                                              <  1:1280237/150‑1 (MQ=255)
                                                                                                            gcagcaATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGAAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGa                                         >  2:701238/1‑150 (MQ=255)
                                                                                                                caATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATAAATACTCTCTCCTCTACGAAGTGTTGTATATAAATTTAGAACTATCATTGAAAAAAATAACTGATAATTATTATTTCAACCTACTGAAtta                                     >  2:93143/1‑149 (MQ=255)
                                                                                                                 aataatTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTc                                    <  2:1579496/150‑1 (MQ=255)
                                                                                                                  ataatTACCCCGGGTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCCGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCa                                   <  1:1708853/150‑1 (MQ=255)
                                                                                                                    aatTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATc                                 >  2:1512855/1‑150 (MQ=255)
                                                                                                                     atTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCt                                >  1:772024/1‑150 (MQ=255)
                                                                                                                       tACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACGTGCTAGTGATTATTTCAACCTACTGAATTTCATCTaa                              >  2:888319/1‑150 (MQ=255)
                                                                                                                             ggTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATATCAACCTACTGAATTTCATCTAAtttttt                        <  2:1778809/150‑1 (MQ=255)
                                                                                                                               ttGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTc                      <  2:1074761/150‑1 (MQ=255)
                                                                                                                               ttGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTc                      <  1:436486/150‑1 (MQ=255)
                                                                                                                                tGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCa                     >  1:267138/1‑150 (MQ=255)
                                                                                                                                   cACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCActa                  >  2:131654/1‑149 (MQ=255)
                                                                                                                                    aCCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCActct                 >  1:769627/1‑150 (MQ=255)
                                                                                                                                    aCCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCActct                 <  1:1266669/150‑1 (MQ=255)
                                                                                                                                      ccGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTAt               <  1:1352459/150‑1 (MQ=255)
                                                                                                                                       cgtaTCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCAGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATg              <  2:877696/147‑1 (MQ=255)
                                                                                                                                       cGGATCATAGTCACTTGATGTGACTATGATACGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATg              <  2:865734/150‑1 (MQ=255)
                                                                                                                                        ggATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATgg             >  2:1115249/1‑150 (MQ=255)
                                                                                                                                        ggATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATATCATCTAATTTTTTTCACTCTATgg             >  2:1742820/1‑150 (MQ=255)
                                                                                                                                        ggATCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATgg             >  2:1777577/1‑150 (MQ=255)
                                                                                                                                         tataaTAGTTACTTGATGTGACTATGATTCTTTTAATACTCTCTCCGCTACGCAGTGTTGTATATCAATTGCGGACTTTAATTGAAATAATTACCTGCTAGTGATTATTTCTACCTACTGAATTTCATCTAATTTTTTTCACTCTTTGGc            <  1:872826/146‑1 (MQ=255)
                                                                                                                                         gATCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGc            >  1:131562/1‑150 (MQ=255)
                                                                                                                                          aTCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCa           >  1:24708/1‑150 (MQ=255)
                                                                                                                                           tCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCaa          >  1:1613405/1‑150 (MQ=255)
                                                                                                                                             aTAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAAt        >  1:1538027/1‑150 (MQ=255)
                                                                                                                                              tAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAAtt       <  2:658318/150‑1 (MQ=255)
                                                                                                                                               aGTCACTTTATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCATTGTTTTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTTAATTTCATCTAATTTTTTTCACTCTATGGCAAATTa      <  1:1225484/150‑1 (MQ=255)
                                                                                                                                               aGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTa      <  1:1054948/150‑1 (MQ=255)
                                                                                                                                               aGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTCTTTTAGATCAATTGCGCACTATCATTGAAATAAAAACCTGCTAGTGAATATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTa      >  2:430861/1‑150 (MQ=255)
                                                                                                                                                gTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAg     >  2:522034/1‑150 (MQ=255)
                                                                                                                                                gTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAg     >  2:543951/1‑150 (MQ=255)
                                                                                                                                                  aaCTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGcc   <  2:1765070/149‑1 (MQ=255)
                                                                                                                                                   aCTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCa  >  2:369521/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCA  >  NC_000913/3657450‑3657746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: