Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,728,215	T→A	5.9%	K386I (AAA→ATA)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,215	0	T	A	5.9%	254.4 / 2.7	90	K386I (AAA→ATA)	xylB	xylulokinase
Reads supporting (aligned to +/- strand):  ref base T (61/23);  new base A (4/2);  total (65/25)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGC  >  NC_000913/3728067‑3728364                                                                                                                                                      |                                                                                                                                                      cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                                                                                                                      <  1:940852/150‑1 (MQ=255)   cgATCTGCGCCAGCCTTGCTGCGCCCAGTGGAGGCCCCACAT‑ACCCCCCCGTACGGTAATCTAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                                                                                                                                                    <  1:1551407/150‑1 (MQ=255)   cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                                                                                                                                                    <  1:726501/150‑1 (MQ=255)              aGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCAGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa                                                                                                                                         <  1:865690/150‑1 (MQ=255)              aGCCTTGCGGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGAAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCAAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa                                                                                                                                         <  1:149397/150‑1 (MQ=255)               gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCTCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGATTAAACCGCaaagtg                                                                                                                                        <  2:1387614/150‑6 (MQ=255)               gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTATTAAACCGCaaagtg                                                                                                                                        <  2:1647525/150‑6 (MQ=255)                ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTTATATCCTCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg                                                                                                                                       <  1:1350887/150‑1 (MQ=255)                              ttGCTGTCCCCACAT‑CCCCCCCCTTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGc                                                                                                                         <  2:148998/149‑1 (MQ=255)                              gTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAGGc                                                                                                                         <  1:1608271/150‑1 (MQ=255)                                    gCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTg                                                                                                                   <  1:693522/150‑1 (MQ=255)                                    gCCCCACAA‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTg                                                                                                                   <  1:1065349/150‑1 (MQ=255)                                     ccccACATCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTg                                                                                                                   <  2:793499/150‑1 (MQ=255)                                          caT‑CCCACCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCTGGTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg                                                                                                             <  1:761463/150‑1 (MQ=255)                                               cccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCACCGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCCTCTGCCAGCGCTTa                                                                                                         >  1:645076/1‑150 (MQ=255)                                                   cccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCACCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAccc                                                                                                     <  1:119558/150‑1 (MQ=255)                                                    ccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCa                                                                                                    <  1:50718/150‑1 (MQ=255)                                                         cGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGTGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCt                                                                                               >  2:741038/1‑150 (MQ=255)                                                         cGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATACCCCCCGcaa                                                                                               >  1:695858/1‑148 (MQ=255)                                                         cGGTAATCGAGCTGCTGACCGCTGATATACGCCAGCATCTGACGCCAGTACTCCCTACGCGCCCCGCCCCCAATCAACGTAACCCTTTGCGGTTTATACCCGCAGGCATGCCCGACATCCATGCCATCTGCAAGAGCATTTCCAACGcgg                                                                                               >  2:1188349/1‑148 (MQ=255)                                                           gTAATCGAGCTGCTGCCCGCTGATATCCGCCAGCATATGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATACCCCCCGCCTTc                                                                                             >  2:1272347/1‑150 (MQ=255)                                                            tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCCCGCCTTcc                                                                                            >  1:86509/1‑150 (MQ=255)                                                            tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTcc                                                                                            <  1:89673/150‑1 (MQ=255)                                                            tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTcc                                                                                            >  2:675312/1‑150 (MQ=255)                                                                 gAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCTCATAACCCCCGCCTTCCAGCAc                                                                                       >  1:453823/1‑150 (MQ=255)                                                                  aGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGATTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCAcc                                                                                      <  1:214988/150‑2 (MQ=255)                                                                   gctgctGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACCTCCATGCCATCTGCCATCGCATAACCCACGCCTTCCAGCACTg                                                                                     <  1:364497/150‑1 (MQ=255)                                                                    ctgctgACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAGCGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGc                                                                                    >  1:1178248/1‑150 (MQ=255)                                                                    ctgctgACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGCCATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGc                                                                                    >  2:1663544/1‑150 (MQ=255)                                                                       ctgACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCCTGCCATCTGCCGGCGCCTAACCCCCGCCTTCCCGCCCTGcgcg                                                                                 >  2:1231873/1‑150 (MQ=255)                                                                         gACCGCTGATATCCGCCAGCATGTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCAGTTTAATACCGCAGGCATGCACGACTTCCATGCCAGCTGCCAGCGCATAACCCACGCCTTCCTGCACTGCTcgcg                                                                               <  2:1724694/150‑1 (MQ=255)                                                                         gACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTcgcg                                                                               >  2:1326853/1‑150 (MQ=255)                                                                         gACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTcgcg                                                                               >  2:1039725/1‑150 (MQ=255)                                                                          aCCGCTGATATCCGCCAGCATCTGACGCCTGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCgcgc                                                                              >  2:1236239/1‑150 (MQ=255)                                                                              cTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTATAATTCCGCAGGCATGCACGACCTCCATGCCATCTGCCAGCGCATAACCCACTCCTGCCAGCACTGCTCGCTcccgg                                                                          >  1:263444/1‑147 (MQ=255)                                                                                gATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTc                                                                        <  2:646350/150‑1 (MQ=255)                                                                                gATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTc                                                                        <  1:1577615/150‑1 (MQ=255)                                                                                  tatCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCAt                                                                      >  2:1243456/1‑150 (MQ=255)                                                                                   atCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCttt                                                                     >  2:310220/1‑150 (MQ=255)                                                                                   atCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCAtt                                                                     >  2:471198/1‑150 (MQ=255)                                                                                    tCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCCTCTGCCAGCGCATACCCAACGCCTTCCAGCACTGCTCGCGCCAGTTCATTg                                                                    >  1:1213094/1‑150 (MQ=255)                                                                                    tCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCCCGCCTTCCAGCACTGCTCGCGCCAGTTCATTg                                                                    >  2:66514/1‑150 (MQ=255)                                                                                     ccGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAACAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGACAGCGCATAACCCACGCCATCCAGCACTGCTCGCGCCAGTTCATTgg                                                                   >  1:614940/1‑150 (MQ=255)                                                                                      cGCCAGCATCTGACGCCAGTACTCCCTACGCGCCCCGCCCCCAATAAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCCTGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTggg                                                                  >  2:811062/1‑150 (MQ=255)                                                                                      cGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACCTCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTggg                                                                  >  1:1817420/1‑150 (MQ=255)                                                                                       gCCTGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTgggg                                                                 <  1:1683548/150‑1 (MQ=255)                                                                                          aGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCa                                                              >  2:1687713/1‑150 (MQ=255)                                                                                               cTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtg                                                         >  1:787236/1‑150 (MQ=255)                                                                                               cTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGGtg                                                         >  2:1439133/1‑150 (MQ=255)                                                                                                tGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCCCGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCAGGTtga                                                        >  2:690088/1‑150 (MQ=255)                                                                                                   cGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATTCCGCAGGCATGCACGACATCCCTGCCATCTGCCAGCGCATAACCAACGCCTTCCAGCACTGCTCGCGCCAGATCATTGGGGCCATGTtgatga                                                     >  1:609788/1‑150 (MQ=255)                                                                                                   cGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtgatga                                                     >  2:954740/1‑150 (MQ=255)                                                                                                   cGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtgatga                                                     >  1:947514/1‑150 (MQ=255)                                                                                                   cGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTtgatga                                                     >  2:755525/1‑150 (MQ=255)                                                                                                    gCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAg                                                    >  1:741379/1‑150 (MQ=255)                                                                                                     ccAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACCCTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACACGCGCCGTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGt                                                   >  1:912905/1‑150 (MQ=255)                                                                                                     ccAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGt                                                   >  1:1525779/1‑150 (MQ=255)                                                                                                           cTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGCCATCCATGCCATCTGCCAGCGCATAACCCCCGCCTTCCCGCCCTGCTCGCGCCAGTTCATTGGGGCCATGGGGATGAGTCAAAcc                                             >  1:749067/1‑150 (MQ=255)                                                                                                            tCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACAGCCATGCCATCTGCCAGCGCATAACCCACGCCGTCCAGCACTGCTCGCGCGAGTTCATTGGGGCCAGGTTGATGGGTCAAAccc                                            >  2:4717/1‑149 (MQ=255)                                                                                                             cACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATCCCGCAGGCATGGCCGGCGGCCCTGCCATCTGCCGGCGCCTACCCCCCGCCTGCCAGCACTGCTCGCGCCAGTTCCGTCGGGCCAGGGTGCTGAGTCAGCCCaa                                           >  1:1111790/1‑150 (MQ=255)                                                                                                             cACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCCCGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCaa                                           >  2:1343523/1‑150 (MQ=255)                                                                                                              aCTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCaaa                                          <  2:1734668/150‑1 (MQ=255)                                                                                                              aCTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCaaa                                          >  1:1734668/1‑150 (MQ=255)                                                                                                               cTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAg                                         >  2:1805838/1‑150 (MQ=255)                                                                                                                  cgcgCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCAGGTTGATGAGTCAAACCAAAGaaa                                      >  1:1557476/1‑150 (MQ=255)                                                                                                                  cgcgCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGGTCATTGGGGCCATTTTGAGGCGTCAAACcaaacaaa                                      >  2:325152/1‑150 (MQ=255)                                                                                                                      ccccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAAGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCAGTGGGGCCATGTTGCTGAGTCAAACCAATGAAAAccc                                  >  1:308428/1‑150 (MQ=255)                                                                                                                       cccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAAGCCATCAGCCAGCGCATAACCCACGCCTTCACGCACAGCTCGCGCCAGTTCATAGGGGCCAAGTTGATGAGTCAAAGAAAATAAAGcccc                                 >  1:872875/1‑150 (MQ=255)                                                                                                                       cccgccccCAATCAACGTAACACTTTGCGGATTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCGAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAAcccc                                 >  2:1069435/1‑150 (MQ=255)                                                                                                                        ccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGCCATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAAccccc                                >  2:234508/1‑150 (MQ=255)                                                                                                                           cccccAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATACCCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTc                             >  1:558519/1‑150 (MQ=255)                                                                                                                           cccccAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAT‑CCCCCCGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCg                            >  2:1564215/1‑150 (MQ=255)                                                                                                                            ccccAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCg                            >  1:1776076/1‑150 (MQ=255)                                                                                                                            ccccAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCg                            >  2:593204/1‑150 (MQ=255)                                                                                                                                aaTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCCCGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTg                        >  1:1614756/1‑150 (MQ=255)                                                                                                                                  tCAACGTCACACTTTGCGGTTTAATACCGCAGGCATGCACGACAGCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTggg                      <  2:1664655/150‑1 (MQ=255)                                                                                                                                    aaCGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGa                    >  1:1137272/1‑150 (MQ=255)                                                                                                                                      cGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAACACCCCCTTCGCCTGGGGatt                  >  1:1807060/1‑150 (MQ=255)                                                                                                                                      cGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCAGCCAGCGCATAACCCACGCCTTCCAGCACTGCTAGCGCCAGTACATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTACGCCTGGGGatt                  >  1:652610/1‑150 (MQ=255)                                                                                                                                        tAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGattat                >  2:1614295/1‑150 (MQ=255)                                                                                                                                        tAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCAAGTTGAAGAGTCACACCAAAGAAAACCCCCTTAGCCTGGGGattat                >  2:1582809/1‑150 (MQ=255)                                                                                                                                          catgcctGCGGTATTAAACCGCAGGCATGCACGACATCCATGCCATCTCCCAGAGCATAACGCACGCCTTCCAGCACTGCGAGCGCCAGTTCAGTGGGGCCATGTTGATGAGTCAACCCAAAGAAACCCGCCTTCGCCGGGGGATGATtg              >  1:280049/7‑150 (MQ=255)                                                                                                                                          acacTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtg              >  1:638586/1‑150 (MQ=255)                                                                                                                                           cacTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgt             >  2:175893/1‑150 (MQ=255)                                                                                                                                             gcctGCGGTATTAAACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgt           >  2:818217/4‑150 (MQ=255)                                                                                                                                             gcctGCGGTATTAAACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCGGGGGATTATtgtgt           >  2:825220/4‑150 (MQ=255)                                                                                                                                               ttGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTgg         <  1:1039725/150‑1 (MQ=255)                                                                                                                                                 gCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCg       >  2:281984/1‑150 (MQ=255)                                                                                                                                                  cGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGt      >  2:1373904/1‑150 (MQ=255)                                                                                                                                                  cGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGt      >  1:1365252/1‑150 (MQ=255)                                                                                                                                                    gTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGTGATTATTGTGTGGCGTAc    >  2:251229/1‑150 (MQ=255)                                                                                                                                                      ttAATACCGCAGGCATGCACGACCTCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCCCTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCGTCGCCTGGGGAGTATTGTGTGGCGTACGc  >  2:1225733/1‑150 (MQ=255)                                                                                                                                                      ttAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCTGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAACCCCCCGTCGCCGGGGGATTATTGTGTGGCGTACGc  >  2:1219656/1‑150 (MQ=255)                                                                                                                                                      ttAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAACCCCCCTTCGCCTGGGGATTTTTGTGTGGCGTACGc  >  1:1146372/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                      CGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGC  >  NC_000913/3728067‑3728364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑