BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,748,110	C→A	6.2%	L343M (CTG→ATG) ‡	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,748,110	0	C	A	6.2%	343.8 / 10.2	113	L343M (CTG→ATG) ‡	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base C (48/58); new base A (2/5); total (50/63)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

Only 100 of 116 total aligned reads displayed.

TGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACT  >  NC_000913/3747962‑3748259

    tttATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTAc                                                                                                                                                  >  1:296014/1‑150 (MQ=255)

    tttATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTAc                                                                                                                                                  <  2:692783/150‑1 (MQ=255)

       aTCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgc                                                                                                                                               <  1:812476/150‑1 (MQ=255)

        tCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgca                                                                                                                                              >  2:246326/1‑150 (MQ=255)

        tCGATCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGTGAAATCTCGGTTGATGAGATCAATCAGGCCGGTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgca                                                                                                                                              <  1:1452862/150‑1 (MQ=255)

         cGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCCGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTATGgcag                                                                                                                                             >  1:1181456/1‑150 (MQ=255)

          gTTGACGTAGCCAGCCCTACCTCTTCCGGCAACCTCCAATGGTTTACCGCAAAGTGGGGAGACATCTCGTTTGATGAGATCAATCAGGCCGGTGCCAGCTTGCCGAAGGCTTGTGGCGATCTCTTTTTCCTGCCGTACAGGAACGgcagg                                                                                                                                            <  1:180513/150‑2 (MQ=255)

          gTTCACGAAGCCCGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTAGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcagc                                                                                                                                            >  1:735182/1‑150 (MQ=255)

          gTTCACGAAGCCAGCCCTACCTCTTCCGGCCACCTCGAATGGTTTACCGCAACGTGGGGCGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGgcagg                                                                                                                                            <  1:876539/150‑2 (MQ=255)

          gTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcagc                                                                                                                                            <  1:738129/150‑1 (MQ=255)

          gTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcagc                                                                                                                                            <  1:738132/150‑1 (MQ=255)

          gTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGgcagg                                                                                                                                            <  2:403831/150‑2 (MQ=255)

             cACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGGCAGGaaa                                                                                                                                         <  1:149195/150‑2 (MQ=255)

             aaCGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGGCAGGaaa                                                                                                                                         <  2:1314424/149‑2 (MQ=255)

               cGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGc                                                                                                                                       <  1:1605253/150‑1 (MQ=255)

               cGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGc                                                                                                                                       <  2:567161/150‑1 (MQ=255)

                 aaGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTGTACCGCCCAGTGGGGAGAAATCACGATTGAAGCGAACAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTTCCGTTCCTGTACGGCAGCCccgccg                                                                                                                                     >  1:885277/1‑150 (MQ=255)

                   gccagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCACCGCCGGa                                                                                                                                   >  2:1001952/1‑150 (MQ=255)

                    ccagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGAc                                                                                                                                  <  1:1288148/150‑1 (MQ=255)

                    ccagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGAc                                                                                                                                  <  2:2450/150‑1 (MQ=255)

                    ccagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGAc                                                                                                                                  <  1:1191359/150‑1 (MQ=255)

                       gccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGAGCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCg                                                                                                                               >  1:1578904/1‑150 (MQ=255)

                         ccTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAAAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCgag                                                                                                                             <  1:752431/150‑1 (MQ=255)

                          cTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCgaga                                                                                                                            <  1:1075913/150‑1 (MQ=255)

                             ccTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCACCGCCGGACTCGAGATGa                                                                                                                         >  1:787946/1‑150 (MQ=255)

                               tctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGCGATGAcc                                                                                                                       >  2:1307127/1‑150 (MQ=255)

                               tctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGAcc                                                                                                                       <  2:1449533/150‑1 (MQ=255)

                               tctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGAcc                                                                                                                       >  1:545340/1‑150 (MQ=255)

                               tctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATCAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGAcc                                                                                                                       <  2:81436/150‑1 (MQ=255)

                                ctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCa                                                                                                                      <  2:363121/150‑1 (MQ=255)

                                 ttCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAg                                                                                                                     <  1:1030405/150‑1 (MQ=255)

                                 ttCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAg                                                                                                                     <  1:1251714/150‑1 (MQ=255)

                                   ccGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTg                                                                                                                   <  2:766812/150‑1 (MQ=255)

                                     ggCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGt                                                                                                                 >  1:56113/1‑150 (MQ=255)

                                     ggCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGt                                                                                                                 >  2:1011015/1‑150 (MQ=255)

                                        aaCCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTc                                                                                                              <  2:1606319/150‑1 (MQ=255)

                                           cTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTAc                                                                                                           <  1:391985/150‑1 (MQ=255)

                                             cGAATGGTTTACCGCACAGTGGGGTGAAATGTCGTGTGATGAGATCAATCAGGCCGGTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGGTCCTGGACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACgg                                                                                                         <  1:478781/150‑1 (MQ=255)

                                             cGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACgg                                                                                                         <  1:753296/150‑1 (MQ=255)

                                              gAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACggg                                                                                                        <  1:633835/150‑1 (MQ=255)

                                              gAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACggg                                                                                                        <  2:296014/150‑1 (MQ=255)

                                              gAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACggg                                                                                                        <  2:571000/150‑1 (MQ=255)

                                               aaTGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGa                                                                                                       <  1:1652559/150‑1 (MQ=255)

                                                  ggTTTACCGCACAGTGTGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGTAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGc                                                                                                    <  1:892900/150‑1 (MQ=255)

                                                   gTTTACAGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGATGCCAGCTTCCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCGGCCCCGCCGGACTCGCGCTGCCCAGTGGGTTGTCCGGGATGcc                                                                                                   >  1:274790/1‑149 (MQ=255)

                                                      tACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGc                                                                                                >  2:1157317/1‑150 (MQ=255)

                                                         ctcacaGTGGGGAGAACTCTCGTATGATGAGATCACTCAGGCCGGTGCCAGCTTGCCTAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGGTTCTACGGGATGCAGTCCAt                                                                                             <  2:159390/148‑1 (MQ=255)

                                                             caGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTcac                                                                                         >  1:240821/1‑150 (MQ=255)

                                                               gTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCacac                                                                                       >  2:762328/1‑150 (MQ=255)

                                                                tGGGGAGCAATCTCGTTTGAAGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACAcc                                                                                      <  1:20773/150‑1 (MQ=255)

                                                                   ggAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgc                                                                                   >  2:1244873/1‑150 (MQ=255)

                                                                      gaAATCTCGTTTGATGAGATCAATCACGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgcgcg                                                                                >  1:1088097/1‑150 (MQ=255)

                                                                       aaaTCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCGTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCgcgcgc                                                                               <  2:1609254/150‑1 (MQ=255)

                                                                        aaTCTCGTTTGATGAGATCAATCGGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCa                                                                              >  1:1613752/1‑150 (MQ=255)

                                                                          tctcGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCAcc                                                                            <  1:10333/150‑1 (MQ=255)

                                                                          tctcGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCAcc                                                                            >  1:1128434/1‑150 (MQ=255)

                                                                                 tgatgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCa                                                                     <  1:1710487/150‑1 (MQ=255)

                                                                                  gatgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAg                                                                    >  1:824225/1‑150 (MQ=255)

                                                                                  gatgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCAGTTCCTGTACGGCAGCAACCCCGGACTCGAGATGACCAGGGGTTTCTACGTGATGCAGGCCATTCAAACCCGCGCGCACCTGTTGCAg                                                                    >  2:326408/1‑150 (MQ=255)

                                                                                   atgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGGTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAgg                                                                   <  1:915206/150‑1 (MQ=255)

                                                                                      agaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGATCCTGTACGGCAGCTACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCGCACCCGCGCGCACCTGTTGCAGGCCa                                                                >  1:137194/1‑150 (MQ=255)

                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGCCTCGAGATGACCAGTGGTTTCTACGGGATGCACGCCATTCGCACCCGCGCGCAACTGTTGCAGGCGAt                                                               >  1:336822/1‑150 (MQ=255)

                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAt                                                               >  2:844111/1‑150 (MQ=255)

                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAt                                                               >  2:1445532/1‑150 (MQ=255)

                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAt                                                               >  1:160437/1‑150 (MQ=255)

                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGCTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAt                                                               >  2:691538/1‑150 (MQ=255)

                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGAAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCTCACCTGTTGAAGGCCAt                                                               >  2:1438314/1‑150 (MQ=255)

                                                                                        atcaatcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATc                                                              <  1:591852/150‑1 (MQ=255)

                                                                                         tcaatcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCt                                                             <  2:1366245/150‑1 (MQ=255)

                                                                                          caatcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTa                                                            <  2:1086587/150‑1 (MQ=255)

                                                                                            atcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATg                                                          <  2:1399087/150‑1 (MQ=255)

                                                                                                    gTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGggtg                                                  <  2:56113/150‑1 (MQ=255)

                                                                                                       gCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACCCCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGgtggtg                                               >  2:1572011/1‑150 (MQ=255)

                                                                                                         cAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGtt                                             <  1:1011015/150‑1 (MQ=255)

                                                                                                          aGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGCTGTTc                                            >  2:1784777/1‑150 (MQ=255)

                                                                                                           gCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCa                                           >  1:964682/1‑150 (MQ=255)

                                                                                                            cTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAg                                          >  1:1177254/1‑150 (MQ=255)

                                                                                                               gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCa                                       <  2:419497/150‑1 (MQ=255)

                                                                                                               gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCa                                       <  1:802128/150‑1 (MQ=255)

                                                                                                               gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGGTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCa                                       <  1:16119/150‑1 (MQ=255)

                                                                                                                ccGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCACCGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCat                                      <  1:1770998/150‑1 (MQ=255)

                                                                                                                ccGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCat                                      <  2:1796175/150‑1 (MQ=255)

                                                                                                                 cGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCata                                     <  1:1519683/150‑1 (MQ=255)

                                                                                                                   aaGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATg                                   >  2:1025345/1‑150 (MQ=255)

                                                                                                                     ggCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAc                                 <  1:1682470/150‑1 (MQ=255)

                                                                                                                       cTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAccc                               >  1:1124798/1‑150 (MQ=255)

                                                                                                                                aTCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACCCCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAAcc                      >  1:843776/1‑150 (MQ=255)

                                                                                                                                    ctTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAAt                  <  1:1729253/150‑1 (MQ=255)

                                                                                                                                     tttttCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGCCCCACCTCAACCGAATg                 >  2:758091/1‑150 (MQ=255)

                                                                                                                                     tttttCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATg                 >  2:1355914/1‑150 (MQ=255)

                                                                                                                                         tCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGcgcg             >  2:896462/1‑150 (MQ=255)

                                                                                                                                          gcTGCCGTACAGGAACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGa            >  2:662126/2‑150 (MQ=255)

                                                                                                                                          ccTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGATGCCGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCGCAACCGAATGcgcgc            >  2:1714166/1‑149 (MQ=255)

                                                                                                                                           cTGCCGTACAGGAACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGCCCCACCTCAACCGAATGCGCGaa           >  2:595891/1‑150 (MQ=255)

                                                                                                                                             gCCGTTCCTGTCCGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACg         <  2:885275/150‑1 (MQ=255)

                                                                                                                                             gCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACGCCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTCGTGTTCAGCCATCTGACCCACCTCCACCGAATGCGCGAACg         >  1:84492/1‑150 (MQ=255)

                                                                                                                                             gCCGGTCCTGTACGGCAGCAACGCCGGACTCGAGATGTCCAGTGGGTGCTACGGGATGCAGGCCATTCACACCCGCGCGGACCTGTTGCAGGCCATCTATGAAGGGGTGGGGTTAAGCCATATGACCCACCTCAACCGAATGCGCGAACg         <  2:885277/150‑1 (MQ=255)

                                                                                                                                                 tttcTGTACGGCAGCAAAGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGtttt     <  2:888767/147‑1 (MQ=255)

                                                                                                                                                   gcTGTACGGCAGCAAGGAAGGACTCGAGTTGACGTGTGGTTTCTACGGGATGCAGGCCTTTCACACCCGAGCGCACCTGTTTCAGGCATTATATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGAGAACGTTTTAc   <  2:1574998/149‑1 (MQ=255)

                                                                                                                                                    cTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACt  <  1:896462/150‑1 (MQ=255)

TGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACT  >  NC_000913/3747962‑3748259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`