Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,748,111 T→G 5.4% L343R (CTG→CGG) ‡ lyxK → L‑xylulose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,748,1110TG 5.4% 349.0 / 6.1 115L343R (CTG→CGG) ‡lyxKL‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base T (47/61);  new base G (2/5);  total (49/66)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTG  >  NC_000913/3747962‑3748260
                                                                                                                                                     |                                                                                                                                                     
tGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCt                                                                                                                                                       >  2:790229/1‑150 (MQ=255)
    tttATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTAc                                                                                                                                                   <  2:692783/150‑1 (MQ=255)
    tttATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTAc                                                                                                                                                   <  1:375363/150‑1 (MQ=255)
       aTCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgc                                                                                                                                                <  1:812476/150‑1 (MQ=255)
       aTCGTTCACGAAGCCAGCCCTACCTCATCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgc                                                                                                                                                <  1:1757874/150‑1 (MQ=255)
        tCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgca                                                                                                                                               >  2:246326/1‑150 (MQ=255)
        tCGATCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGTGAAATCTCGGTTGATGAGATCAATCAGGCCGGTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgca                                                                                                                                               <  1:1452862/150‑1 (MQ=255)
         cGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCCGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTATGgcag                                                                                                                                              >  1:1181456/1‑150 (MQ=255)
          gTTGACGTAGCCAGCCCTACCTCTTCCGGCAACCTCCAATGGTTTACCGCAAAGTGGGGAGACATCTCGTTTGATGAGATCAATCAGGCCGGTGCCAGCTTGCCGAAGGCTTGTGGCGATCTCTTTTTCCTGCCGTACAGGAACGgcagg                                                                                                                                             <  1:180513/150‑2 (MQ=255)
          gTTCACGAAGCCCGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTAGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcagc                                                                                                                                             >  1:735182/1‑150 (MQ=255)
          gTTCACGAAGCCAGCCCTACCTCTTCCGGCCACCTCGAATGGTTTACCGCAACGTGGGGCGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGgcagg                                                                                                                                             <  1:876539/150‑2 (MQ=255)
          gTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcagc                                                                                                                                             <  1:738132/150‑1 (MQ=255)
          gTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcagc                                                                                                                                             <  1:738129/150‑1 (MQ=255)
          gTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGgcagg                                                                                                                                             <  2:403831/150‑2 (MQ=255)
            tCACGAAGCCAGCCCTTCCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTCCTGCCGTTCCTGTACGGCAGCAAc                                                                                                                                          >  2:637461/1‑150 (MQ=255)
             cACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGGCAGGaaa                                                                                                                                          <  1:149195/150‑2 (MQ=255)
             aaCGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGGCAGGaaa                                                                                                                                          <  2:1314424/149‑2 (MQ=255)
               cGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGc                                                                                                                                        <  2:567161/150‑1 (MQ=255)
                 aaGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCCGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGTGCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCg                                                                                                                                      >  1:885275/1‑150 (MQ=255)
                 aaGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTGTACCGCCCAGTGGGGAGAAATCACGATTGAAGCGAACAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTTCCGTTCCTGTACGGCAGCCccgccg                                                                                                                                      >  1:885277/1‑150 (MQ=255)
                   gccagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCACCGCCGGa                                                                                                                                    >  2:1001952/1‑150 (MQ=255)
                    ccagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGAc                                                                                                                                   <  1:1288148/150‑1 (MQ=255)
                    ccagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGAc                                                                                                                                   <  1:1191359/150‑1 (MQ=255)
                       gccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCg                                                                                                                                <  2:1531548/150‑1 (MQ=255)
                       gccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGAGCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCg                                                                                                                                >  1:1578904/1‑150 (MQ=255)
                         ccTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAAAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCgag                                                                                                                              <  1:752431/150‑1 (MQ=255)
                          cTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCgaga                                                                                                                             <  1:1075913/150‑1 (MQ=255)
                             ccTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCACCGCCGGACTCGAGATGa                                                                                                                          >  1:787946/1‑150 (MQ=255)
                               tctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGCGATGAcc                                                                                                                        >  2:1307127/1‑150 (MQ=255)
                               tctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGAcc                                                                                                                        <  2:1449533/150‑1 (MQ=255)
                               tctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGAcc                                                                                                                        >  1:545340/1‑150 (MQ=255)
                                ctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCa                                                                                                                       <  2:363121/150‑1 (MQ=255)
                                 ttCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAg                                                                                                                      <  1:1030405/150‑1 (MQ=255)
                                 ttCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAg                                                                                                                      <  1:1251714/150‑1 (MQ=255)
                                   ccGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTg                                                                                                                    <  1:789291/150‑1 (MQ=255)
                                   ccGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTg                                                                                                                    <  2:766812/150‑1 (MQ=255)
                                     ggCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGt                                                                                                                  >  1:56113/1‑150 (MQ=255)
                                     ggCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGt                                                                                                                  >  2:1011015/1‑150 (MQ=255)
                                        aaCCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTc                                                                                                               <  2:1606319/150‑1 (MQ=255)
                                           cTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTAc                                                                                                            <  1:391985/150‑1 (MQ=255)
                                           cTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTAc                                                                                                            >  2:1719353/1‑150 (MQ=255)
                                           cTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTAc                                                                                                            <  2:1023217/150‑1 (MQ=255)
                                             cGAATGGTTTACCGCACAGTGGGGTGAAATGTCGTGTGATGAGATCAATCAGGCCGGTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGGTCCTGGACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACgg                                                                                                          <  1:478781/150‑1 (MQ=255)
                                              gAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACggg                                                                                                         <  1:633835/150‑1 (MQ=255)
                                              gAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACggg                                                                                                         <  2:296014/150‑1 (MQ=255)
                                              gAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACggg                                                                                                         <  2:571000/150‑1 (MQ=255)
                                               aaTGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGa                                                                                                        <  1:1652559/150‑1 (MQ=255)
                                                  ggTTTACCGCACAGTGTGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGTAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGc                                                                                                     <  1:892900/150‑1 (MQ=255)
                                                   gTTTACAGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGATGCCAGCTTCCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCGGCCCCGCCGGACTCGCGCTGCCCAGTGGGTTGTCCGGGATGcc                                                                                                    >  1:274790/1‑149 (MQ=255)
                                                             caGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTcac                                                                                          >  1:240821/1‑150 (MQ=255)
                                                               gTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCacac                                                                                        >  2:762328/1‑150 (MQ=255)
                                                                tGGGGAGCAATCTCGTTTGAAGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACAcc                                                                                       <  1:20773/150‑1 (MQ=255)
                                                                   ggAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgc                                                                                    >  2:1244873/1‑150 (MQ=255)
                                                                      gaAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgcgcg                                                                                 >  2:388593/1‑150 (MQ=255)
                                                                      gaAATCTCGTTTGATGAGATCAATCACGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgcgcg                                                                                 >  1:1088097/1‑150 (MQ=255)
                                                                       aaaTCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCGTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCgcgcgc                                                                                <  2:1609254/150‑1 (MQ=255)
                                                                        aaTCTCGTTTGATGAGATCAATCGGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCa                                                                               >  1:1613752/1‑150 (MQ=255)
                                                                          tctcGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCAcc                                                                             <  1:10333/150‑1 (MQ=255)
                                                                                ttGATGAGATCAATCAGGCCGTAGCCAGCTTGCCGAAGGCTGGGGGCGCTCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGc                                                                       <  2:1331229/150‑1 (MQ=255)
                                                                                 tgatgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCa                                                                      <  1:1710487/150‑1 (MQ=255)
                                                                                  gatgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAg                                                                     >  1:824225/1‑150 (MQ=255)
                                                                                  gatgaGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCAGTTCCTGTACGGCAGCAACCCCGGACTCGAGATGACCAGGGGTTTCTACGTGATGCAGGCCATTCAAACCCGCGCGCACCTGTTGCAg                                                                     >  2:326408/1‑150 (MQ=255)
                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGCCTCGAGATGACCAGTGGTTTCTACGGGATGCACGCCATTCGCACCCGCGCGCAACTGTTGCAGGCGAt                                                                >  1:336822/1‑150 (MQ=255)
                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAt                                                                >  2:844111/1‑150 (MQ=255)
                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAt                                                                >  2:1445532/1‑150 (MQ=255)
                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAt                                                                >  1:160437/1‑150 (MQ=255)
                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGCTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAt                                                                >  2:691538/1‑150 (MQ=255)
                                                                                       gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGAAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCTCACCTGTTGAAGGCCAt                                                                >  2:1438314/1‑150 (MQ=255)
                                                                                         tcaatcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCt                                                              <  2:1366245/150‑1 (MQ=255)
                                                                                          caatcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTa                                                             <  2:1086587/150‑1 (MQ=255)
                                                                                                    gTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGggtg                                                   <  2:56113/150‑1 (MQ=255)
                                                                                                       gCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACCCCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGgtggtg                                                >  2:1572011/1‑150 (MQ=255)
                                                                                                         cAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGtt                                              <  1:1011015/150‑1 (MQ=255)
                                                                                                          aGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGCTGTTc                                             >  2:1784777/1‑150 (MQ=255)
                                                                                                           gCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCa                                            >  1:964682/1‑150 (MQ=255)
                                                                                                            cTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAg                                           >  1:1177254/1‑150 (MQ=255)
                                                                                                               gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCa                                        <  1:802128/150‑1 (MQ=255)
                                                                                                               gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGGTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCa                                        <  1:16119/150‑1 (MQ=255)
                                                                                                                ccGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCACCGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCat                                       <  1:1770998/150‑1 (MQ=255)
                                                                                                                ccGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCat                                       <  2:1796175/150‑1 (MQ=255)
                                                                                                                 cGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCata                                      <  1:1519683/150‑1 (MQ=255)
                                                                                                                   aaGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATg                                    >  2:1025345/1‑150 (MQ=255)
                                                                                                                     ggCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAc                                  <  1:1682470/150‑1 (MQ=255)
                                                                                                                       cTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAccc                                >  1:1124798/1‑150 (MQ=255)
                                                                                                                          ggggCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTAAGGGATGCAGGCCATTCACACCCGCGCGCCCCTGTTGCAGGCCTTCTATGAAGGGGTGGTGTTCAGCCATATGACCCAcc                             >  1:1161204/1‑150 (MQ=255)
                                                                                                                                aTCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACCCCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAAcc                       >  1:843776/1‑150 (MQ=255)
                                                                                                                                     tttttCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGCCCCACCTCAACCGAATg                  >  2:758091/1‑150 (MQ=255)
                                                                                                                                     tttttCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATg                  >  2:1355914/1‑150 (MQ=255)
                                                                                                                                      ttttCCTGCCGTTCCTGTACGGCAGCAACGCCGGGCTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGCCCCCCCTCACCCGAATgc                 >  1:699865/1‑150 (MQ=255)
                                                                                                                                        ttCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATgcgc               <  1:1610767/150‑1 (MQ=255)
                                                                                                                                         tCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGcgcg              >  2:896462/1‑150 (MQ=255)
                                                                                                                                          ccTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGATGCCGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCGCAACCGAATGcgcgc             >  2:1714166/1‑149 (MQ=255)
                                                                                                                                           cTGCCGTACAGGAACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGCCCCACCTCAACCGAATGCGCGaa            >  2:595891/1‑150 (MQ=255)
                                                                                                                                             gCCGTTCCTGTCCGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACg          <  2:885275/150‑1 (MQ=255)
                                                                                                                                             gCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTAGGGGATGCAGGCCTTTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCTGCCATATGACCCACCTCAACCGAATGCGCGAACg          <  1:139189/150‑1 (MQ=255)
                                                                                                                                             gCCGGTCCTGTACGGCAGCAACGCCGGACTCGAGATGTCCAGTGGGTGCTACGGGATGCAGGCCATTCACACCCGCGCGGACCTGTTGCAGGCCATCTATGAAGGGGTGGGGTTAAGCCATATGACCCACCTCAACCGAATGCGCGAACg          <  2:885277/150‑1 (MQ=255)
                                                                                                                                                 tttcTGTACGGCAGCAAAGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGtttt      <  2:888767/147‑1 (MQ=255)
                                                                                                                                                   gcTGTACGGCAGCAAGGAAGGACTCGAGTTGACGTGTGGTTTCTACGGGATGCAGGCCTTTCACACCCGAGCGCACCTGTTTCAGGCATTATATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGAGAACGTTTTAc    <  2:1574998/149‑1 (MQ=255)
                                                                                                                                                    cTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACt   <  1:896462/150‑1 (MQ=255)
                                                                                                                                                     tGTACGTCAGAAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTg  <  2:49494/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTG  >  NC_000913/3747962‑3748260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: