Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,757,942 C→A 7.3% intergenic (‑115/+75) yiaY ← / ← selB L‑threonine dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,757,9420CA 7.3% 374.8 / 12.6 135intergenic (‑115/+75)yiaY/selBL‑threonine dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor
Reads supporting (aligned to +/- strand):  ref base C (72/52);  new base A (8/3);  total (80/55)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

Only 100 of 138 total aligned reads displayed.

CACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTC  >  NC_000913/3757795‑3758091
                                                                                                                                                   |                                                                                                                                                      
cacaGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCAt                                                                                                                                                      <  1:1652457/150‑1 (MQ=255)
cacaGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCAt                                                                                                                                                      >  2:199567/1‑150 (MQ=255)
aacaGAAGGAATAAAGAAAGTTGAAGCTGCCATAACACACTCCTGAAAGTTTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCAt                                                                                                                                                      <  1:925888/149‑1 (MQ=255)
 acaGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCAtt                                                                                                                                                     >  1:633512/1‑150 (MQ=255)
       ggAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACaa                                                                                                                                              >  1:40738/1‑150 (MQ=255)
       ggAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACaa                                                                                                                                              <  1:574472/150‑1 (MQ=255)
       ggAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACaa                                                                                                                                              >  1:1580324/1‑150 (MQ=255)
       ggAATAAAGAACGTTGAAGCTGCCATAACACACTACTTAAAGTGTAAAAAAGTGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACaa                                                                                                                                              <  1:1742484/150‑1 (MQ=255)
        gAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAAt                                                                                                                                             >  1:82298/1‑150 (MQ=255)
        gAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATACATTAAACAAt                                                                                                                                             >  2:155295/1‑150 (MQ=255)
         aaTAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGAACATTGTATTCATTAAACAAtg                                                                                                                                            >  1:1167157/1‑150 (MQ=255)
            aaaGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTTTAATGAATACAATGTGc                                                                                                                                         <  2:1445372/150‑1 (MQ=255)
             aaGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCt                                                                                                                                        >  2:1677867/1‑150 (MQ=255)
              aGAAAGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTAATGAGGATTTTCATTCGAAAATATGATCTGAATTAATGATTATCAAAATTAATCTAATAAAAAGCACATTTTATTCATTAAACAATTTGCtt                                                                                                                                       >  2:128536/1‑150 (MQ=255)
                   gTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCttttttt                                                                                                                                  >  2:581816/1‑150 (MQ=255)
                   gTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCttttttt                                                                                                                                  >  1:1077179/1‑150 (MQ=255)
                       aaGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCTAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAAt                                                                                                                              <  1:813140/150‑1 (MQ=255)
                         gCTGCCATAACACACTCCTGAAAGTGAAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGATTTTTTTAAAtta                                                                                                                            >  2:275608/1‑149 (MQ=255)
                          cTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCa                                                                                                                           <  1:581656/150‑1 (MQ=255)
                            gCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATa                                                                                                                         >  2:1465545/1‑150 (MQ=255)
                                 aaCACAATCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGTATTTACATTCGATATTATGATCAGTATTATTGATTATAAAAATTAATCTAATAAAAAGATAATTGTATAAATTATTAAATCTGATTTTTTTAAATTCATAAGatt                                                                                                                    >  1:299429/1‑148 (MQ=255)
                                  acacacTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTAATTAAACAATGTGCTTTTTTTAAATTCATAAGAAtt                                                                                                                   >  2:273718/1‑150 (MQ=255)
                                  acacacTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTACATACGAAAATATGATCAGTATTATTGATTATCAAAATTAAACTAATAAAAAGCACATTGTATTCATTAAAAAATGTCATTTTTTTAAATTAATAATAAtt                                                                                                                   >  1:637714/1‑150 (MQ=255)
                                    acacTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAAtttt                                                                                                                 >  2:1350122/1‑150 (MQ=255)
                                     cacTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTg                                                                                                                <  2:624309/150‑1 (MQ=255)
                                      aatacttaaaGTGTAAAAAATGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGt                                                                                                               <  2:509471/143‑1 (MQ=255)
                                       cTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTa                                                                                                              <  2:712946/150‑1 (MQ=255)
                                        tCCTGAAAGTGTAAAAAAGGGCGGACAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTAt                                                                                                             >  2:1276648/1‑150 (MQ=255)
                                          cTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTa                                                                                                           >  1:839822/1‑150 (MQ=255)
                                                  gtAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAatttattt                                                                                                   >  2:1708982/1‑150 (MQ=255)
                                                   tAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTAtttt                                                                                                  <  2:771099/150‑1 (MQ=255)
                                                   tAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTAtttt                                                                                                  >  1:1019391/1‑150 (MQ=255)
                                                   tAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTAtttt                                                                                                  >  1:1298104/1‑150 (MQ=255)
                                                   tAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTATTAAATTCATAAGAATTTTGTATTAATTTAtttt                                                                                                  >  2:725442/1‑150 (MQ=255)
                                                    aaaaaaGGTCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTa                                                                                                 <  1:1606439/150‑1 (MQ=255)
                                                     aaaaaGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTaa                                                                                                >  2:1156226/1‑150 (MQ=255)
                                                     aaaaaGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTaa                                                                                                >  2:790175/1‑150 (MQ=255)
                                                     aaaaaGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTaa                                                                                                <  1:378562/150‑1 (MQ=255)
                                                        aaGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAAc                                                                                             <  2:940749/150‑1 (MQ=255)
                                                          gggcgggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATGTAATAAAAAGCACATTGTATTAATTAAACTATTTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACtt                                                                                           <  2:391590/150‑1 (MQ=255)
                                                           ggcgggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACtta                                                                                          >  1:705947/1‑150 (MQ=255)
                                                            gcgggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCAAAAGAATTTTTTATTAATTTATTTTAAAACttaa                                                                                         >  1:1385848/1‑150 (MQ=255)
                                                               ggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACttaatta                                                                                      >  2:1251526/1‑150 (MQ=255)
                                                               ggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACttaatta                                                                                      <  1:1503161/150‑1 (MQ=255)
                                                                   gCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCa                                                                                  >  2:1617024/1‑150 (MQ=255)
                                                                              aTTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTAttt                                                                       >  2:525736/1‑150 (MQ=255)
                                                                              aTTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTAttt                                                                       <  1:1129233/150‑1 (MQ=255)
                                                                               ttttCATTCGAAAATATGTTTATTATTATTTATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTAtttt                                                                      <  2:782351/150‑1 (MQ=255)
                                                                                  tCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTcc                                                                   <  2:342387/150‑1 (MQ=255)
                                                                                   cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                                  <  1:49640/150‑1 (MQ=255)
                                                                                   cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                                  <  2:777098/150‑1 (MQ=255)
                                                                                   cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                                  <  2:209663/150‑1 (MQ=255)
                                                                                   cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                                  <  2:82298/150‑1 (MQ=255)
                                                                                    aTTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCATTATTTTTcagt                                                                 >  1:488945/1‑147 (MQ=255)
                                                                                    aTTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCAAATTGTATTCAATAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCgg                                                                 >  2:594841/1‑150 (MQ=255)
                                                                                      tCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGaa                                                               >  2:528606/1‑150 (MQ=255)
                                                                                      tCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATAATTTCATTATTTTTCAGGaa                                                               >  1:760721/1‑150 (MQ=255)
                                                                                      tCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGGGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGaa                                                               <  2:1139340/150‑1 (MQ=255)
                                                                                      acGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCATTATTTTTACGGaa                                                               >  1:760729/2‑150 (MQ=255)
                                                                                       cGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCATTATTTTTCCTGaaa                                                              >  1:1540373/1‑150 (MQ=255)
                                                                                        gAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCTGAaat                                                             >  2:1124952/1‑150 (MQ=255)
                                                                                          aaaTATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAaataat                                                          <  1:1475167/150‑1 (MQ=255)
                                                                                              atGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAaataataat                                                       <  1:75085/150‑1 (MQ=255)
                                                                                                       attattGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCgcgc                                              <  1:622794/150‑1 (MQ=255)
                                                                                                             gATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCGATaaa                                        >  2:1732768/1‑150 (MQ=255)
                                                                                                             gATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATaaa                                        >  1:1618526/1‑150 (MQ=255)
                                                                                                             gATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATaaa                                        >  2:1748428/1‑150 (MQ=255)
                                                                                                             gATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGAGCAATaaa                                        >  1:903372/1‑150 (MQ=255)
                                                                                                             aaTTATCAAAATTAATCTAATAAAAAGCACATTGTAATAATTAAACAATGTGCTTTATATAAATAAATAAGAATTTTGTATTAATTTAAATTAAAAATTAATTAATCATTTCCTTATTTTTCCGGAAAAAATAATGCGACGCGCAATaaa                                        >  1:903370/2‑150 (MQ=255)
                                                                                                               ttATCAAAATTAATCTAATAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGa                                     <  2:1616861/150‑1 (MQ=255)
                                                                                                                tATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGa                                     >  2:1140588/1‑150 (MQ=255)
                                                                                                                tATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGa                                     <  1:595958/150‑1 (MQ=255)
                                                                                                                 aTCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGAt                                    <  2:1580324/150‑1 (MQ=255)
                                                                                                                   cAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCa                                  >  2:636321/1‑150 (MQ=255)
                                                                                                                   aaaaaTTAATCTAATAAAAAGCACTTTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCa                                  <  2:850020/149‑1 (MQ=255)
                                                                                                                   aaaaaTTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCa                                  <  1:523702/149‑1 (MQ=255)
                                                                                                                    aaaaTTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATAAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCAt                                 >  2:372857/1‑150 (MQ=255)
                                                                                                                      aaTTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCAttt                               >  1:1733443/1‑150 (MQ=255)
                                                                                                                           aTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacg                          >  1:656700/1‑150 (MQ=255)
                                                                                                                           aTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacg                          <  1:1029805/150‑1 (MQ=255)
                                                                                                                            tCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacga                         >  2:1745686/1‑150 (MQ=255)
                                                                                                                             cTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgac                        >  1:617387/1‑150 (MQ=255)
                                                                                                                             cTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTACCGGAAATAATAATGCTTCGCGCAATAAATCATCATTTCCacgac                        >  1:1458873/1‑150 (MQ=255)
                                                                                                                              taataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGTAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacg                       >  1:1325268/1‑150 (MQ=255)
                                                                                                                               taaaaaaaGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacga                      >  2:1057652/4‑150 (MQ=38)
                                                                                                                                ataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTACGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacgac                     >  2:281534/1‑150 (MQ=255)
                                                                                                                                 taaAAAGCACATTTTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGTATTTTGTATTAATTTAATTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacgacg                    <  1:316744/150‑1 (MQ=255)
                                                                                                                                  aaaaaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGAcgc                   >  2:1042248/1‑150 (MQ=255)
                                                                                                                                    aaaGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGt                 >  2:1528825/1‑150 (MQ=255)
                                                                                                                                     aaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTa                <  1:1209783/150‑1 (MQ=255)
                                                                                                                                     aaGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTa                >  1:703561/1‑150 (MQ=255)
                                                                                                                                      aGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa               >  2:412643/1‑150 (MQ=255)
                                                                                                                                      aGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa               >  2:1029959/1‑150 (MQ=255)
                                                                                                                                       gCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCAGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACTAGTaaa              >  1:363697/1‑150 (MQ=255)
                                                                                                                                         acaTTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGc            >  2:406457/1‑150 (MQ=255)
                                                                                                                                              gTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATg       >  2:719867/1‑150 (MQ=255)
                                                                                                                                                aTTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCg     >  2:207653/1‑150 (MQ=255)
                                                                                                                                                 ttCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCgg    <  2:1392433/150‑1 (MQ=255)
                                                                                                                                                   cATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTATTTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTc  >  2:131378/1‑150 (MQ=255)
                                                                                                                                                   cATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTc  >  2:723822/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
CACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTC  >  NC_000913/3757795‑3758091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: