Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,760,792 T→G 6.3% N153T (AAT→ACT)  selA ← selenocysteine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,760,7920TG 6.3% 299.3 / 6.2 110N153T (AAT→ACT) selAselenocysteine synthase
Reads supporting (aligned to +/- strand):  ref base T (66/36);  new base G (6/2);  total (72/38)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TCCCTACTTCGTGTAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACC  >  NC_000913/3760646‑3760938
                                                                                                                                                  |                                                                                                                                                  
tCCCTACTTCGTGTAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTGCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCAgtgt                                                                                                                                                 >  1:703273/1‑150 (MQ=255)
 cccTACTTCGTGTAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGtt                                                                                                                                                >  2:450169/1‑150 (MQ=255)
 cccTACTTCGTGTAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCCCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATAGtt                                                                                                                                                >  1:1467515/1‑150 (MQ=255)
  ccTACTTCGTGTAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTa                                                                                                                                               >  2:491736/1‑150 (MQ=255)
    tACTTCGTGTAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTAtt                                                                                                                                             <  2:1146815/150‑1 (MQ=255)
    tACTTCGTGTAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTAtt                                                                                                                                             <  2:203562/150‑1 (MQ=255)
     aCTTCGTGTAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTg                                                                                                                                            <  2:1596569/150‑1 (MQ=255)
       ttCGTGTAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGAc                                                                                                                                          <  1:1698089/150‑1 (MQ=255)
          gtgtTGGGTGCAGCCTGCCTGACGCATAAAATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGATCGCCGCGAGATACGACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCGGCATTGTTATTGACGAt                                                                                                                                       <  2:1387867/150‑1 (MQ=255)
          gtgtAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGAt                                                                                                                                       <  1:821388/150‑1 (MQ=255)
            gtAGGGTGCAGCCTGACTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATAc                                                                                                                                     <  2:390834/150‑1 (MQ=255)
             tAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACAATGCGGCGGCGGTGTTATTGatgttggc                                                                                                                                    <  1:387636/150‑8 (MQ=38)
                   gCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATc                                                                                                                              >  1:709059/1‑150 (MQ=255)
                     aGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCtt                                                                                                                            >  1:1268222/1‑150 (MQ=255)
                       cctgcctgACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGCGATACCACCACCTCTTTTCCGCTGGCGGTGGCCGCCAACATCAATAACACCGCCGCCGCAGTGTTATTGACGATACAGGCATCTTcc                                                                                                                          >  2:668106/1‑150 (MQ=255)
                       cctgcctgACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTcc                                                                                                                          >  2:681580/1‑150 (MQ=255)
                       cctgcctgACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTcc                                                                                                                          <  2:1041009/150‑1 (MQ=255)
                       cctgcctgACGCATAACATCGGGAATACCAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTcc                                                                                                                          <  1:809737/150‑1 (MQ=255)
                         tgcctgACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGc                                                                                                                        <  1:1827043/150‑1 (MQ=255)
                         tgcctgACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGc                                                                                                                        >  2:1724054/1‑150 (MQ=255)
                           cctgACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGccc                                                                                                                      <  2:724402/150‑1 (MQ=255)
                              gACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCg                                                                                                                   <  1:1025626/150‑1 (MQ=255)
                                  cATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCCCCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCAGTGTTTTTGACGATACAGGCATCTTCCGCACCCGTAAt                                                                                                               >  2:187442/1‑150 (MQ=255)
                                      aCATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACgg                                                                                                           <  1:1302636/150‑1 (MQ=255)
                                         tCGGGAATACGAAACGCGCCGCCAATCTCCACCAGATCGCCGCGAGATACCACCACCTCTTATCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGcac                                                                                                        <  2:1392892/150‑1 (MQ=255)
                                           gggAATATGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTATCCCCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCAcag                                                                                                      >  2:831961/1‑150 (MQ=255)
                                            ggAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGGGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCAcagc                                                                                                     <  1:1177084/150‑1 (MQ=255)
                                               aTACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCTCCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACagcagc                                                                                                  <  2:1055507/150‑1 (MQ=255)
                                               aTACGAAACGCGCCGCCAATCTCCACCAGATCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACagcagc                                                                                                  >  2:1004995/1‑150 (MQ=255)
                                                   gAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACCGGCATCTTCCGCCCCCGGGATACGGCACAGCAGATgcg                                                                                              >  2:319770/1‑150 (MQ=255)
                                                    aaaCGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTgcgc                                                                                             >  2:780344/1‑150 (MQ=255)
                                                         cgccgaCAATCTCCACCACTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGTCACAGCAGCTGCGCAAgcg                                                                                        <  1:283945/150‑1 (MQ=255)
                                                          gccgccAATCTCCACAAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAgcgc                                                                                       >  1:931036/1‑150 (MQ=255)
                                                           ccgccAATCTCCCCCAGTTCGCCGCGAGCTACCACCCCCTCTTTTCCGCTGGCAGCGGCCGCCAACATCAATAACGCCGCCGCCGCATTGTAATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCCCCTGCGCCAGCGcc                                                                                      >  2:91438/1‑150 (MQ=255)
                                                           ccgccAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCAGCCAACATCAATAACACCGCCGCCGCTTTGTTATTGCCGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGcc                                                                                      >  2:1111686/1‑150 (MQ=255)
                                                            cgccAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCGTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACATTCATCTTCCGCCCCCGTAATACGGCACATCAGCTGCGCCAGCGccc                                                                                     >  2:854096/1‑150 (MQ=255)
                                                               cAATCTCCACCAGTTCGCCGCCAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCACCATCAATAACACCGCCGCCGCATTGTTATTGCCGATACAGGCATCTTCCGCCCCCGTAATACGGCACCGCAGCTGCGCCAGCGCCCgga                                                                                  >  2:1519243/1‑148 (MQ=255)
                                                                 aTCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATcg                                                                                >  1:544636/1‑150 (MQ=255)
                                                                  tctcCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATAAGGCACAGCAGCTGCGCCAGCGCCCGATcgc                                                                               >  2:942453/1‑150 (MQ=255)
                                                                   ctcCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATcgcg                                                                              <  2:168354/150‑1 (MQ=255)
                                                                   ctcCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATcgcg                                                                              >  1:983927/1‑150 (MQ=255)
                                                                     ccaccaGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGAt                                                                            >  2:945901/1‑150 (MQ=255)
                                                                      caccaGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATg                                                                           >  2:597928/1‑150 (MQ=255)
                                                                       accaGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGt                                                                          >  2:452060/1‑150 (MQ=255)
                                                                          aGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACCCCGCCGCCGCATTGTTATTTACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCcg                                                                       >  2:1459150/1‑150 (MQ=255)
                                                                           gTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACCGCAGCTGCGCCCGCGCCCGATCGCGATGTCcgc                                                                      >  2:668090/1‑150 (MQ=255)
                                                                           gTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCcgc                                                                      >  1:1581633/1‑150 (MQ=255)
                                                                            gtCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACAGCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCcgcg                                                                     <  1:269819/149‑1 (MQ=255)
                                                                             tCGCCGCGAGAAACCACCACCTCTATTCCGCTCGCAGTGGACGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATAATCCACAGCAGCTGCACCAGCGCCCGATCGCGATCTCcgcct                                                                    >  2:1738718/1‑148 (MQ=255)
                                                                               gccgcGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTcc                                                                  >  2:559955/1‑150 (MQ=255)
                                                                                ccgcGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCg                                                                 >  2:1604868/1‑150 (MQ=255)
                                                                                ccgcGAGATACCACCACCTCTTATCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCAGAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCg                                                                 <  1:216045/150‑1 (MQ=255)
                                                                                      gaTACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCACCATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCCCAGCAGCTGCGCCAGCGCCCGAGCGCGATGTCCGCGTCCGGcgtcg                                                           >  2:167469/1‑150 (MQ=255)
                                                                                       aTACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGcgtcgg                                                          >  1:1520347/1‑149 (MQ=255)
                                                                                        tACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCTACATCAATAACACCGCCGCCGCATTGTTATAGACGCTACAGGCCTCTTCCGCCCCCGTACTACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGCTGTCCGCGTCCGGCgtcgtc                                                         >  1:262960/1‑150 (MQ=255)
                                                                                         accaccaccTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTcc                                                        >  2:1652916/1‑150 (MQ=255)
                                                                                         accaccaccTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTcc                                                        >  1:72479/1‑150 (MQ=255)
                                                                                          ccaccaccTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCa                                                       >  2:229953/1‑150 (MQ=255)
                                                                                           caccaccTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCAGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGTCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAg                                                      >  1:863262/1‑150 (MQ=255)
                                                                                            accaccTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGa                                                     >  1:1609962/1‑150 (MQ=255)
                                                                                             ccaccTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACCCCGCCGCCGCAGTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCCGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGAt                                                    <  2:866488/150‑1 (MQ=255)
                                                                                             ccaccTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGAt                                                    <  1:681580/150‑1 (MQ=255)
                                                                                                   ctTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCCGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATaag                                              >  2:1545416/1‑148 (MQ=255)
                                                                                                    ttttCCGCTGGCAGTGGCCGCCAACATCAATAACCCCGCCGCCGCATTGTTATTGACGATACAGGCAGCTTCCGCTCCCGTAATACGGGACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATAccc                                             <  1:237108/150‑3 (MQ=255)
                                                                                                     tttCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCg                                            >  1:1677629/1‑150 (MQ=255)
                                                                                                     tttCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCg                                            >  1:52934/1‑150 (MQ=255)
                                                                                                        ccGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAgg                                         >  1:171238/1‑150 (MQ=255)
                                                                                                         cGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAggg                                        >  1:1817774/1‑150 (MQ=255)
                                                                                                         cGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAggg                                        >  1:1250627/1‑150 (MQ=255)
                                                                                                            tGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTcc                                     >  1:1242939/1‑149 (MQ=255)
                                                                                                               cAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTg                                  <  1:540414/150‑1 (MQ=255)
                                                                                                                 gTGGCCGCCAACATCAATAACCCCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGc                                <  2:1268222/150‑1 (MQ=255)
                                                                                                                   ggCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGa                              >  1:425278/1‑150 (MQ=255)
                                                                                                                    gccgccAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGaa                             >  2:220641/1‑150 (MQ=255)
                                                                                                                     ccgccAACTTCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCTCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAAc                            <  2:1138839/150‑1 (MQ=255)
                                                                                                                     ccgccAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACCGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAAc                            >  1:1197485/1‑150 (MQ=255)
                                                                                                                     ccgccAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAAc                            >  2:1365241/1‑150 (MQ=255)
                                                                                                                     ccgccAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAAc                            <  1:491736/150‑1 (MQ=255)
                                                                                                                      cgccAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACg                           <  1:1264188/150‑1 (MQ=255)
                                                                                                                       gccAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGc                          <  1:1724054/150‑1 (MQ=255)
                                                                                                                        ccAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGTGTCACTGGCGAACGCa                         >  2:187616/1‑150 (MQ=255)
                                                                                                                          aaCATCAATAACACCGCCGCCGCATTGTTATTGCCGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATa                       <  2:1467515/150‑1 (MQ=255)
                                                                                                                           tcgtCAATAACAATGCGGCGGCGGTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACGCGAGGGTCACTGGCGAACGCATAg                      >  2:1357691/4‑150 (MQ=255)
                                                                                                                             gtCAATAACAATGCGGCGGCGGTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGcc                    >  1:425721/2‑150 (MQ=255)
                                                                                                                             aTCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGCTCATACTCGAGGGTCACTGGCGAACGCATAGcc                    >  1:148727/1‑150 (MQ=255)
                                                                                                                               cAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGTACGCATAGCCTg                  >  2:748234/1‑150 (MQ=255)
                                                                                                                               cAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGcccg                  <  2:544053/150‑3 (MQ=255)
                                                                                                                                aaTAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGCACGCATAGCCTgc                 >  2:415956/1‑150 (MQ=255)
                                                                                                                                  tAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTgcgc               >  1:905638/1‑150 (MQ=255)
                                                                                                                                    acacCGCCGCCGCATTGTTATTGGCGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCCCTGGCGAACGCATAGCCTGCGCaa             <  1:1336519/150‑1 (MQ=255)
                                                                                                                                     cacCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAAc            >  1:1338331/1‑150 (MQ=255)
                                                                                                                                      acCGCCGCCTCATTGTTATTGACGATACAGTCATATTCCGCCCCCGTAATACGGCACACCAGCTGCGCCCGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATAATACTAGAGGGTCACTCGCGAACGCATAGCCTGCGCAAcc           >  1:462841/1‑149 (MQ=255)
                                                                                                                                       ccgccgccgCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGGCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACgg          <  1:450169/150‑1 (MQ=255)
                                                                                                                                         gccgccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCt        <  1:945901/150‑1 (MQ=255)
                                                                                                                                          ccgccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGACCGCATAGCCTGCGCAACGGCtt       >  2:1502300/1‑150 (MQ=255)
                                                                                                                                          ccgccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCtt       >  1:1779548/1‑150 (MQ=255)
                                                                                                                                          ccgccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCtt       <  2:1686025/150‑1 (MQ=255)
                                                                                                                                            gccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCCTAGCCTGCGCACCGGCTTcc     >  1:1821145/1‑150 (MQ=255)
                                                                                                                                             ccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTccc    >  2:1747316/1‑149 (MQ=255)
                                                                                                                                               gcATTGTTATTGACGATCCAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGCACGCATAGCCTGCGCAACGGCTTCCAcc  >  2:317668/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCCCTACTTCGTGTAGGGTGCAGCCTGCCTGACGCATAACATCGGGAATACGAAACGCGCCGCCAATCTCCACCAGTTCGCCGCGAGATACCACCACCTCTTTTCCGCTGGCAGTGGCCGCCAACATCAATAACACCGCCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACC  >  NC_000913/3760646‑3760938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑