Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,792,767 A→T 5.9% intergenic (‑216/+59) kbl ← / ← yibB glycine C‑acetyltransferase/YibB family protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,792,7670AT 5.9% 379.7 / 9.6 129intergenic (‑216/+59)kbl/yibBglycine C‑acetyltransferase/YibB family protein, function unknown
Reads supporting (aligned to +/- strand):  ref base A (55/65);  new base T (4/4);  total (59/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

Only 100 of 131 total aligned reads displayed.

GCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCC  >  NC_000913/3792619‑3792915
                                                                                                                                                    |                                                                                                                                                    
 cgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAg                                                                                                                                                    >  2:859720/1‑150 (MQ=255)
   ttAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACCGCATTGATATAAAAAGAAAAAATGATGCCAAAGca                                                                                                                                                  <  1:1280815/150‑1 (MQ=255)
      acacTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGcatca                                                                                                                                               >  2:734052/1‑150 (MQ=255)
      acacTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGcatca                                                                                                                                               >  2:1732051/1‑150 (MQ=255)
         cTTCTGCAAAATTCAGGATAAATAACGTGTAACTTAATGGCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCAttt                                                                                                                                            <  2:273431/150‑1 (MQ=255)
          ttCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCAtttt                                                                                                                                           <  1:701518/150‑1 (MQ=255)
           tCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCAttttt                                                                                                                                          >  1:845989/1‑150 (MQ=255)
            cTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTc                                                                                                                                         <  2:570643/150‑1 (MQ=255)
             tGCAAAATTCAGGATAAATATCGTGTATCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATTATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttttttc                                                                                                                                        <  1:1450446/150‑3 (MQ=255)
              gCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCATTTTTTCt                                                                                                                                       <  2:478491/150‑1 (MQ=255)
              gCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCt                                                                                                                                       >  1:1545317/1‑150 (MQ=255)
               cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTa                                                                                                                                      <  2:435083/150‑1 (MQ=255)
                 aaaTTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTAtc                                                                                                                                    <  1:516116/150‑1 (MQ=255)
                 aaaTTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGAATGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCATTTTTTCtttt                                                                                                                                    <  1:1385426/150‑4 (MQ=255)
                    ttCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCa                                                                                                                                 >  2:147731/1‑150 (MQ=255)
                     tCAGGATAAATAACGTGTAACTTGATGTCTCTCAACCCCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGACAAAGCATCATTTTTCCTATCTCAg                                                                                                                                >  1:1521873/1‑150 (MQ=255)
                      cAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGt                                                                                                                               >  2:914024/1‑150 (MQ=255)
                       aGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGtt                                                                                                                              >  2:637398/1‑150 (MQ=255)
                        ggATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTg                                                                                                                             <  2:1786266/150‑1 (MQ=255)
                        ggATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTg                                                                                                                             >  1:1678614/1‑150 (MQ=255)
                              ataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCa                                                                                                                       >  1:1042671/1‑150 (MQ=255)
                              ataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCa                                                                                                                       <  2:1058320/150‑1 (MQ=255)
                                   gtgtAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATAGGTTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACg                                                                                                                  <  2:1690355/150‑1 (MQ=255)
                                    tgtAACTTGATGTCTCTAAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGc                                                                                                                 <  2:1752190/150‑1 (MQ=255)
                                     gtAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCt                                                                                                                <  2:1545317/150‑1 (MQ=255)
                                       aaCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTAc                                                                                                              <  2:1249108/150‑1 (MQ=255)
                                         cTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTAccc                                                                                                            <  1:1365707/150‑1 (MQ=255)
                                            gATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACt                                                                                                         <  2:511808/150‑1 (MQ=255)
                                                tctctcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATg                                                                                                     <  1:593522/150‑1 (MQ=255)
                                                   ctcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAg                                                                                                  >  2:615103/1‑150 (MQ=255)
                                                     cAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAggg                                                                                                >  1:1367493/1‑150 (MQ=255)
                                                     cAACACATACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATTATTGATTTTACTGCTTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCTCTAATGGAggg                                                                                                <  2:559647/150‑1 (MQ=255)
                                                        cacCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGaaa                                                                                             >  2:1046376/1‑150 (MQ=255)
                                                          ccTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAAcc                                                                                           <  2:854143/150‑1 (MQ=255)
                                                          ccTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAATTGGTATCAAAACGCTACCCACTAATGGAGGGAAAcc                                                                                           >  1:380754/1‑150 (MQ=255)
                                                                  tagtagAAAAACTGTTCAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCt                                                                                   <  1:95834/150‑1 (MQ=255)
                                                                    gtagAAAAACTGTACAGTTTTGGCTGGGCAAAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTg                                                                                 <  1:1674473/150‑1 (MQ=255)
                                                                     tagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                                <  2:62639/150‑1 (MQ=255)
                                                                     tagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTATCCTATCTCAGTTGGTATAAAAACGCTACCCACTAATGGAGGGAAACCTAttttaatga                                                                                >  2:316612/1‑145 (MQ=255)
                                                                      agAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGaa                                                                               <  1:450034/150‑1 (MQ=255)
                                                                      agAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGaa                                                                               >  1:1369974/1‑150 (MQ=255)
                                                                        aaaaaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGTATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAg                                                                             <  2:460229/150‑1 (MQ=255)
                                                                        aaaaaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAg                                                                             <  2:980353/150‑1 (MQ=255)
                                                                        aaaaaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAg                                                                             <  2:856702/150‑1 (MQ=255)
                                                                             cTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGaaaa                                                                        >  2:735913/1‑150 (MQ=255)
                                                                               gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAAtt                                                                      >  1:1137065/1‑150 (MQ=255)
                                                                               gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAAtt                                                                      >  2:524542/1‑150 (MQ=255)
                                                                                tACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATtc                                                                     <  2:1305121/150‑1 (MQ=255)
                                                                                    gTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAg                                                                 <  2:1778527/150‑1 (MQ=255)
                                                                                    gTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAg                                                                 >  2:1571999/1‑150 (MQ=255)
                                                                                          gCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTg                                                           <  1:1130640/150‑1 (MQ=255)
                                                                                          gCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACACACTAATGGAGGGAAACCTATTTTCTTGAAATGAAAATTCTCAGCTccat                                                           >  1:1637441/1‑148 (MQ=255)
                                                                                            tGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCa                                                         >  1:1201432/1‑150 (MQ=255)
                                                                                             gggCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAt                                                        >  1:1150221/1‑150 (MQ=255)
                                                                                               gCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTAcc                                                                                                             >  1:763236/1‑95 (MQ=255)
                                                                                               gCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTAcc                                                                                                             <  2:763236/95‑1 (MQ=255)
                                                                                                  tAATTATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTg                                                   <  2:1660346/150‑1 (MQ=255)
                                                                                                  tAATGATGATTGTTTTACTGCATTGATATAAAAAGAAAAAATGATGCTAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGc                                                  >  1:1808547/1‑150 (MQ=255)
                                                                                                  aaaTGATGATTGATTTTACTGCATTGATATAAAAAGTAAAAAAGATGACAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAAACTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTg                                                   <  2:93052/149‑1 (MQ=255)
                                                                                                     tgatgaTTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGccc                                                <  1:1035725/150‑1 (MQ=255)
                                                                                                      tatgatTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGcccc                                               <  2:1664041/149‑1 (MQ=255)
                                                                                                      tatgatTGATTTTAAGGCATTGATCTAAAAAGAAAAAATGATGCCACATCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTAACCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGcccc                                               <  2:294564/149‑1 (MQ=255)
                                                                                                      gatgatTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACATATTTTCTTGAAAGGAAAATTAACAGCGCCTGCATTTTTGcaca                                               >  1:1367893/1‑147 (MQ=255)
                                                                                                          attgattTTACTGCATTGGTATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaaa                                           >  2:977606/1‑150 (MQ=255)
                                                                                                          attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaaa                                           <  1:282604/150‑1 (MQ=255)
                                                                                                            tgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgt                                         <  2:903081/150‑1 (MQ=255)
                                                                                                              attTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                       >  2:1405977/1‑150 (MQ=255)
                                                                                                               ttttACTGCATTGATATAAAAAGAGAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACATATTTTCTTGAAAGGAAAATTATCAGCGCCTGCATTTTTGCAACTAAAGTGtt                                      >  2:904935/1‑150 (MQ=255)
                                                                                                                tttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                     >  1:630294/1‑150 (MQ=255)
                                                                                                                 ttACTGCATTGATATAAAAAGAAAAAATTATGCCAAAGCATCATTTTTCCTATCTCCGTTGGTATCAACACGCTAACCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCGCAGCGCCTGCATTTTTGCCCCTAAAGTGTTAc                                    <  1:316612/150‑1 (MQ=255)
                                                                                                                 ttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTAc                                    <  1:891892/150‑1 (MQ=255)
                                                                                                                  tACTTCATTGTTATAAAAAGAAAAAAAGCAGCCAAAGCATCATTATTCCTATCTCAGATGGTATCAAATCGCTACACACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACt                                   <  2:343103/150‑1 (MQ=255)
                                                                                                                  tACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACt                                   <  1:147731/150‑1 (MQ=255)
                                                                                                                  tACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTATCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACt                                   <  2:343104/150‑1 (MQ=255)
                                                                                                                    cTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTcc                                 <  1:637398/150‑1 (MQ=255)
                                                                                                                       cATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGa                              <  2:1045854/150‑1 (MQ=255)
                                                                                                                        aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGaa                             <  1:914024/150‑1 (MQ=255)
                                                                                                                         tttatatAAAAATAAAAAATGAAGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCAAAAATGGAGTGAACAATATTTTCGTGAAAGGAAAATACTAAGCGAATAAATTTTTGCCACTAAAGTCTTAATACTGAag                            >  2:279176/4‑150 (MQ=255)
                                                                                                                         ttGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCATTTGGTATCAAAAAGCTACCCACAAATGGAGGGAAACATATTTTATTGAAAGGAAAATTCTCAGAGCCTGCATTTTTGCCCATAAAGTGTTAATCATGaat                            >  1:626824/1‑149 (MQ=255)
                                                                                                                         ttGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAag                            <  1:944723/150‑1 (MQ=255)
                                                                                                                          tGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAagc                           >  2:1348872/1‑150 (MQ=255)
                                                                                                                          tGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTATTGAAAGGAAAATTCTCAGAGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAagc                           >  1:124310/1‑150 (MQ=255)
                                                                                                                              ataAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCg                       <  1:1802794/150‑1 (MQ=255)
                                                                                                                               taAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGa                      >  1:1462719/1‑150 (MQ=255)
                                                                                                                               taAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGa                      >  1:951888/1‑150 (MQ=255)
                                                                                                                                aaaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGTTATCAAAACGCTACCCACTAATGGTGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCGGAAGCAGCGaa                     >  2:212856/1‑150 (MQ=255)
                                                                                                                                aaaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGaa                     >  2:634345/1‑150 (MQ=255)
                                                                                                                                 aaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGaaa                    >  1:1346805/1‑150 (MQ=255)
                                                                                                                                   taggaaaaaTGATGCTTTGGCATCATTTTTCCTATCTCAGTTGGTATCAACACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTTAAGTGTTGCTCCTGAAGCAGGGAGACa                  >  1:1177071/5‑150 (MQ=255)
                                                                                                                                   aaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACa                  >  1:530033/1‑150 (MQ=255)
                                                                                                                                    aGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACaa                 >  1:123486/1‑150 (MQ=255)
                                                                                                                                     gAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAATCCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCACTAAAGTGTTACTCCTGAAGCAGCGAATCaca                >  1:787855/1‑148 (MQ=255)
                                                                                                                                      gaaaaaTGATGCTTTGGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAAt               >  1:1735370/2‑150 (MQ=255)
                                                                                                                                      gaaaaaTGATGCTTTGGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAAt               >  1:1077932/2‑150 (MQ=255)
                                                                                                                                      aaaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAAt               >  2:265478/1‑150 (MQ=255)
                                                                                                                                       aaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATc              <  2:1662609/150‑1 (MQ=255)
                                                                                                                                       aaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAATTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATc              <  2:380754/150‑1 (MQ=255)
                                                                                                                                          aaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACTCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGTAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGaa           <  1:194872/150‑1 (MQ=255)
                                                                                                                                              atgCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGAGCATGCATTTTTGCCCCTAAAGTGTTACTCATGAAGCAGCGAAACAAATCGCACCaa       >  2:55619/1‑149 (MQ=255)
                                                                                                                                                   aaaGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTcc  <  1:859720/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCC  >  NC_000913/3792619‑3792915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: