Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,809,359 T→G 6.3% F274V (TTC→GTC) ‡ waaA → 3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,809,3590TG 6.3% 349.5 / 11.3 123F274V (TTC→GTC) ‡waaA3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)
Reads supporting (aligned to +/- strand):  ref base T (52/62);  new base G (5/3);  total (57/66)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

Only 100 of 125 total aligned reads displayed.

GCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTT  >  NC_000913/3809211‑3809507
                                                                                                                                                    |                                                                                                                                                    
gcgcCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCtt                                                                                                                                                     >  2:730989/1‑150 (MQ=255)
gcgcCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGAGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCtt                                                                                                                                                     >  2:1079210/1‑150 (MQ=255)
  gccgcgAGTGGGGTCCACACCGCGCGGTTTTGATTGCCACCAGCACTCACGAAGGCGCTGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTcc                                                                                                                                                   <  1:1468579/150‑1 (MQ=255)
      ccAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCGTCATCCGGAACGCTTCCCGGAt                                                                                                                                              >  1:1806890/1‑150 (MQ=255)
       cAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGGAAGCGTTCCGGAt                                                                                                                                              <  2:306592/150‑1 (MQ=255)
        aGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATAAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATg                                                                                                                                             >  2:1121686/1‑150 (MQ=255)
         gTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGc                                                                                                                                            >  1:267033/1‑150 (MQ=255)
          ggggCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCg                                                                                                                                           <  1:225284/149‑1 (MQ=255)
           gggCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGa                                                                                                                                          <  2:736955/150‑1 (MQ=255)
           gggCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGa                                                                                                                                          >  2:648407/1‑150 (MQ=255)
            ggCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGGAAGCGTTCCGGATgacgg                                                                                                                                         <  1:783355/150‑5 (MQ=255)
            ggCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGAt                                                                                                                                         >  2:701102/1‑150 (MQ=255)
             gCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGAtt                                                                                                                                        >  1:1291/1‑150 (MQ=255)
             gCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGAtt                                                                                                                                        >  1:8270/1‑150 (MQ=255)
              caccacACCGCCCGGTATGGATTGCCACCAGCACTTACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTa                                                                                                                                       >  1:580273/1‑150 (MQ=255)
              caccacACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACCTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTa                                                                                                                                       >  1:63198/1‑150 (MQ=255)
              caccacACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGGAAGCGTTCCGGATgacgggg                                                                                                                                       <  1:238604/150‑7 (MQ=255)
                ccacACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAAc                                                                                                                                     >  1:1403395/1‑150 (MQ=255)
                 cacaCCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAAcc                                                                                                                                    <  2:1372201/150‑1 (MQ=255)
                 cacaCCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAAcc                                                                                                                                    <  1:75169/150‑1 (MQ=255)
                  acacCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCt                                                                                                                                   >  2:375226/1‑150 (MQ=255)
                    acCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTg                                                                                                                                 <  1:226700/150‑1 (MQ=255)
                         ccGGTATGGATTGCCACCAGCACTCACGAAGGCTAAGAGAGTGTGGTGATCGCCGCACATCAGGCATGGTTACAGCATTTCGCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAAAGCTTCCCGGATGCGATTACCCTTGTCCGc                                                                                                                            >  2:1215891/1‑150 (MQ=255)
                           ggTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCa                                                                                                                          >  1:436253/1‑150 (MQ=255)
                              aTGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATAAGGCATTGTTACAGAAATTCCAGAATTTATTGCTCATCCTTGTACCCCGTCATCCGGAACCCTTCCCGGATGAGATTAACCTTTTCCGCCAGGc                                                                                                                       >  2:346673/1‑150 (MQ=255)
                                ggATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTg                                                                                                                     >  1:177483/1‑150 (MQ=255)
                                 gATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTgg                                                                                                                    >  2:1315962/1‑150 (MQ=255)
                                 gATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTgg                                                                                                                    <  2:1510991/150‑1 (MQ=255)
                                 gATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTgg                                                                                                                    >  1:1475281/1‑150 (MQ=255)
                                   ttGCCACCATCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGAc                                                                                                                  <  1:1557141/150‑1 (MQ=255)
                                   taggcaccaGCACTCACGTAGGCGACGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACATCAATTCCCGAATTTATTGCTCATCCTGGGACCCCGTCATCGGGATCGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGAc                                                                                                                  <  1:716704/146‑1 (MQ=255)
                                       caccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTaac                                                                                                              >  1:406146/1‑149 (MQ=255)
                                        accaGCACTCACGAATGCGAAGAGAGTTTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCGCTAATTTATTGGTCATCCTTGTACCCCTTCATCCGTAACGCTTCCCGGATGCGTTTAACCTTGTCCGCCAGGCTGGACTAAGc                                                                                                             <  1:346698/150‑1 (MQ=255)
                                        accaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGc                                                                                                             <  1:620055/150‑1 (MQ=255)
                                        accaGCACTCACGAAGGAGAAGAGAGTTTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGc                                                                                                             <  1:245801/150‑1 (MQ=255)
                                         ccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTCCCCCGTCATCCGTAACGCTTGCCGGATGAGATTAACCTTGTCCGCCAGGCTGTACTAAGCt                                                                                                            >  2:1115720/1‑150 (MQ=255)
                                         ccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCt                                                                                                            >  1:1709901/1‑150 (MQ=255)
                                            gCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCtata                                                                                                         >  2:195869/1‑150 (MQ=255)
                                             cACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCTGGCTGGACTAAGCTatat                                                                                                        <  1:1799649/150‑1 (MQ=255)
                                             cACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTatat                                                                                                        <  1:861121/150‑1 (MQ=255)
                                              aCTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATc                                                                                                       >  2:1728765/1‑150 (MQ=255)
                                               cTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATca                                                                                                      >  2:893228/1‑150 (MQ=255)
                                                tCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATcac                                                                                                     <  1:1346727/150‑1 (MQ=255)
                                                 cACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATcaca                                                                                                    <  1:1213990/150‑1 (MQ=255)
                                                   cGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCGTTATTTATTGATCATCCTTGTACCCCGTCATCCGGGCCGCGTCCCGGATGCGATTGTCCTTGTCCGCGAGGCTGGACTCAGCGATATcgctcg                                                                                                  >  2:1112121/1‑145 (MQ=255)
                                                   cGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATACTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCAAGGCTGGACTAAGCTATATCACACg                                                                                                  >  1:1313121/1‑150 (MQ=255)
                                                    gAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGc                                                                                                 <  1:1415744/150‑1 (MQ=255)
                                                    gAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCATCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGc                                                                                                 <  1:1663796/150‑1 (MQ=255)
                                                        gCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCTCACGCTCtt                                                                                             >  1:276673/1‑150 (MQ=255)
                                                             gagagTGTGGTGATCGCCGCACAGCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAggg                                                                                        <  2:1319196/150‑1 (MQ=255)
                                                              agagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAgggg                                                                                       <  1:730989/150‑1 (MQ=255)
                                                                 gtgtGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAg                                                                                    <  2:645722/150‑1 (MQ=255)
                                                                 gtgtGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAg                                                                                    >  1:1448933/1‑150 (MQ=255)
                                                                    tggtgATCGCCGCACATCAGGCATTGTTACAGCAGTTCCCGAATTTATTGCTCATCCTGGTACCCCGTCCTCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTTCGCTATATCACACGCTCTTCAGGGGAAGTcc                                                                                 >  2:361334/1‑150 (MQ=255)
                                                                     ggtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTccc                                                                                >  2:535155/1‑150 (MQ=255)
                                                                      gtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTcccc                                                                               <  1:1025978/150‑1 (MQ=255)
                                                                        gATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTc                                                                             <  1:1042702/150‑1 (MQ=255)
                                                                        gATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCGTTATCACACGCTCTTCAGGGGAAGTCCCCTc                                                                             >  2:950650/1‑150 (MQ=255)
                                                                          tCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTccc                                                                           >  2:920979/1‑149 (MQ=255)
                                                                            gccgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTccacc                                                                         >  1:185399/1‑150 (MQ=255)
                                                                                cacaTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCa                                                                     <  1:535155/150‑1 (MQ=255)
                                                                                      cggCTTTGTTACAGCAATTCCCGAATTTATTGCTGATCCTGGGACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAgg                                                               <  2:1697398/149‑1 (MQ=255)
                                                                                      aGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAgg                                                               <  1:1540897/150‑1 (MQ=255)
                                                                                      aGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGt                                                              <  2:1806890/150‑1 (MQ=255)
                                                                                       ggCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAggg                                                              >  2:1463350/1‑149 (MQ=255)
                                                                                          aTTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTtgt                                                           <  1:1027033/150‑1 (MQ=255)
                                                                                           ttGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTtgtg                                                          <  1:135068/150‑1 (MQ=255)
                                                                                            tGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTgg                                                         <  2:1384463/150‑1 (MQ=255)
                                                                                               tACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTg                                                      >  1:982654/1‑150 (MQ=255)
                                                                                                 cAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGc                                                    <  1:1079210/150‑1 (MQ=255)
                                                                                                 cAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCAGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTACCCTACACCAGCACGCAGGTTGTGGTTGGc                                                    <  1:199320/150‑1 (MQ=255)
                                                                                                   gCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGa                                                  >  2:828376/1‑150 (MQ=255)
                                                                                                    cAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGAt                                                 <  2:1381419/150‑1 (MQ=255)
                                                                                                    cAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGAt                                                 <  1:471816/150‑1 (MQ=255)
                                                                                                      aTTCCCGAATTTATTGCTCATCCTGGTTCCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTGCACCAGCACGCAGGTTGTGGTTGGCGATAc                                               <  2:1500053/150‑1 (MQ=255)
                                                                                                          ccGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTTTCCGCCATCATGGACTAAGCTATATCACACGCTCTTAAGGGGAAGTCCACTCCACCAGCACGAAGGATGTGTGTGGCGATACGagg                                           >  1:367169/1‑148 (MQ=255)
                                                                                                          ccGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTTTTGTTGGCGATACCATg                                           >  2:1144826/1‑150 (MQ=255)
                                                                                                          ccGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATg                                           <  1:654176/150‑1 (MQ=255)
                                                                                                          ccGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGAGGTTGGCGATACGATg                                           <  2:1709901/150‑1 (MQ=255)
                                                                                                                 tATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGt                                    <  1:850008/150‑1 (MQ=255)
                                                                                                                 tATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGt                                    >  2:629118/1‑150 (MQ=255)
                                                                                                                  aTTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGtt                                   <  2:446591/150‑1 (MQ=255)
                                                                                                                     gCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATTCGATGGGCGAgatgat                                >  2:1809022/1‑150 (MQ=255)
                                                                                                                      cTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCGGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATg                               <  2:1680310/150‑1 (MQ=255)
                                                                                                                       tCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGtt                             <  1:995869/150‑1 (MQ=255)
                                                                                                                          tCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTAc                           <  1:1508614/150‑1 (MQ=255)
                                                                                                                            cTGGTACACCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACCCGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCTATACGATGGGCGAGTTGATGtttatg                         >  1:1256092/1‑146 (MQ=255)
                                                                                                                              ggTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCAGACGCACTACAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGGTGGCGATACGATGGGCGAGTTGATGTTACTGTa                       >  2:1297322/1‑150 (MQ=255)
                                                                                                                                tACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATg                     >  2:1433858/1‑150 (MQ=255)
                                                                                                                                    ccGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCAt                 >  1:1142011/1‑150 (MQ=255)
                                                                                                                                    atcgcATCCGGGAAGCGTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCAt                 >  2:638694/5‑150 (MQ=255)
                                                                                                                                    atcgcATCCGGGAAGCGTTCCGGATGCGATTAACCTTGTCCGCCAGGCTCGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCAt                 >  1:1684772/5‑150 (MQ=255)
                                                                                                                                      cgcATCCGGGAAGCGTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTg               >  2:593230/3‑150 (MQ=255)
                                                                                                                                       gcATCCGGGAAGCGTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGc              >  1:1589174/2‑150 (MQ=255)
                                                                                                                                           ccGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGAt          <  1:893228/150‑1 (MQ=255)
                                                                                                                                            cGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGAtc         >  2:968793/1‑150 (MQ=255)
                                                                                                                                               aaCGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCCGCCCGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTTCTGTATGGCATTGCCGATCTCg      >  2:1786865/1‑150 (MQ=255)
                                                                                                                                                 cGCTTCCCGGATGCGATTAACCTTGTCCGCCAGTCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGcc    <  2:1403395/150‑1 (MQ=255)
                                                                                                                                                 cGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGcc    <  2:550211/150‑1 (MQ=255)
                                                                                                                                                   cTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCtt  >  2:1295292/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTT  >  NC_000913/3809211‑3809507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: