Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 441,069 A→T 5.7% V11V (GTT→GTA) ‡ ispA ← geranyltranstransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913441,0690AT 5.7% 404.4 / 6.8 132V11V (GTT→GTA) ‡ispAgeranyltranstransferase
Reads supporting (aligned to +/- strand):  ref base A (60/64);  new base T (6/2);  total (66/66)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

CGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGC  >  NC_000913/440921‑441215
                                                                                                                                                    |                                                                                                                                                  
cGCCGAACATATGACAGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAACCGGCTCAGCGCCTGGTTGGCCTGCTTaa                                                                                                                                                   >  1:564357/1‑150 (MQ=255)
 gCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGTTAGGACTTCTTTAc                                                                                                                                                  >  2:240485/1‑150 (MQ=255)
  ccGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCTCGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACg                                                                                                                                                 >  2:1603144/1‑150 (MQ=255)
  ccGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGAACACGGGAGTGTTCTGAAAGGGCAGTGGGGAGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACg                                                                                                                                                 >  1:920161/1‑150 (MQ=255)
    gAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCa                                                                                                                                               <  2:800935/150‑1 (MQ=255)
       cATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGc                                                                                                                                            >  2:46889/1‑150 (MQ=255)
         tatGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCtt                                                                                                                                          >  2:1530090/1‑150 (MQ=255)
         tatGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCtt                                                                                                                                          >  2:1537234/1‑150 (MQ=255)
         aatGACCGGTGGCATAAACCAGGAAAGGGCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCGTTAAGCAGGcca                                                                                                                                          <  1:246571/149‑3 (MQ=255)
          atGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTc                                                                                                                                         >  1:1605577/1‑150 (MQ=255)
           tGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGTCTGCTTAACGCAGGCTTCg                                                                                                                                        <  1:1159602/150‑1 (MQ=255)
           tGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTTACGCAGGCTTCg                                                                                                                                        >  1:1616600/1‑150 (MQ=255)
           tGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCg                                                                                                                                        <  1:41210/150‑1 (MQ=255)
           tGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCg                                                                                                                                        <  1:1690484/150‑1 (MQ=255)
           tGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCg                                                                                                                                        <  1:35435/150‑1 (MQ=255)
           tGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCg                                                                                                                                        <  2:554235/150‑1 (MQ=255)
           tGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGCGAGTGATCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTAGGCCTGCGTAACGCAGGCTTCg                                                                                                                                        >  1:1281112/1‑150 (MQ=255)
           tGACCGGTGGCATAAACCAGGAAAGGGCGCAGGCGCTTACCACCTAATAATGCGCCATACAGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCg                                                                                                                                        <  1:931169/150‑1 (MQ=255)
            gACCGGTGGCATAAACCAGGAAAGGTCGCCGGCGCTTACCACCTATTAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATATAACGGCTCAGCGCCTGGTTGGCCTGCGTTAAGCAGGccaacc                                                                                                                                       <  1:638880/150‑6 (MQ=255)
            gACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGa                                                                                                                                       <  1:693352/150‑1 (MQ=255)
             aCCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATACTGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAg                                                                                                                                      <  1:636087/150‑1 (MQ=255)
                ggTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTtg                                                                                                                                   >  2:655098/1‑150 (MQ=255)
                 gTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTGCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTtgc                                                                                                                                  <  1:586824/150‑1 (MQ=255)
                 gTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACAGCATGGTTTCGACCACGGGAGTGTTCTCAAAGGGCAGTGGGGAGATAAAACGGCTCCGCGCCTGGTTGGGATGCTTAACGCAGGCTTCGAGTtgc                                                                                                                                  >  2:700206/1‑150 (MQ=255)
                   ggCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTtgctg                                                                                                                                <  2:798330/150‑1 (MQ=255)
                    gCATAAACCAGGAAAGTTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTtgctgc                                                                                                                               <  1:1683187/150‑1 (MQ=255)
                     cATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCg                                                                                                                              >  1:1819142/1‑150 (MQ=255)
                     cATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCg                                                                                                                              <  2:502378/150‑1 (MQ=255)
                           ccAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGt                                                                                                                        >  2:759024/1‑150 (MQ=255)
                           ccAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGt                                                                                                                        <  1:1183485/150‑1 (MQ=255)
                            cAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTc                                                                                                                       <  1:385943/150‑1 (MQ=255)
                                aaaGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCCCGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCAtt                                                                                                                   >  1:509137/1‑150 (MQ=255)
                                  aGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTAc                                                                                                                 <  2:741444/150‑1 (MQ=255)
                                      cGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCAt                                                                                                             >  2:1121327/1‑150 (MQ=255)
                                          ggCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGCCCACGGGAGTGTTCTGCAAGGGCAGTGGGGCGATAAACCGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAACGTCCCTTACTCCTTGTc                                                                                                         >  2:278662/1‑150 (MQ=255)
                                          ggCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGGTGCTGCGGAAAGTCCATTACTCATTGTc                                                                                                         >  1:861771/1‑150 (MQ=255)
                                           gcgcTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTcc                                                                                                        <  1:1000046/150‑1 (MQ=255)
                                            cgcTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCg                                                                                                       <  1:1022407/150‑1 (MQ=255)
                                             gcgtACCACCTCATAATGCGCCATACTGCATGGGTGCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCGCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCgg                                                                                                      <  2:774400/147‑1 (MQ=255)
                                                tACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCGGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGgtgt                                                                                                   <  1:1062628/150‑1 (MQ=255)
                                                  ccaccTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTaa                                                                                                 >  1:458355/1‑150 (MQ=255)
                                                      ataataaTGCGCCATACTGCATGGTTGCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAgg                                                                                             <  1:87841/149‑1 (MQ=255)
                                                       taataaTGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAggg                                                                                            >  1:934203/1‑150 (MQ=255)
                                                           attGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGTAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCGATTACTCATTGTCCGGTGTAAAAGGGGTTa                                                                                        <  1:1306499/148‑1 (MQ=255)
                                                            atGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTag                                                                                       >  2:1241207/1‑150 (MQ=255)
                                                                 ccATACTGCATGGTTACGACCCCGGGAGTGTGCTGAAAGGGCTGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAgg                                                                                  <  2:137170/150‑1 (MQ=255)
                                                                   aTACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGGTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCg                                                                                <  2:651183/150‑1 (MQ=255)
                                                                      cTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTcg                                                                             >  2:1530081/1‑149 (MQ=255)
                                                                        gCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTc                                                                           >  1:988010/1‑150 (MQ=255)
                                                                         cATGGTTTCGACCACGGGAGGGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGTCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGGGTAAAAGGGGTTAGAGAGGCGTCTTCa                                                                          <  1:743752/150‑1 (MQ=255)
                                                                               ttCGACCACGGGAGGGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCa                                                                    <  2:253930/150‑1 (MQ=255)
                                                                                tCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAg                                                                   <  1:1629501/150‑1 (MQ=255)
                                                                                 cGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGa                                                                  >  1:796464/1‑150 (MQ=255)
                                                                                  gACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGAc                                                                 >  1:1225475/1‑150 (MQ=255)
                                                                                   aCCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGAca                                                                >  2:1475334/1‑150 (MQ=255)
                                                                                   aCCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGAca                                                                <  1:245623/150‑1 (MQ=255)
                                                                                     cACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCAGTACTCATTGTACGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATAGTCATAcagc                                                              >  1:1096423/1‑150 (MQ=255)
                                                                                       cgtgAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTATAAGGGGTTAGAGAGGCGTCTTCATTGTCAGAcagcag                                                            <  2:1633446/147‑1 (MQ=255)
                                                                                       cGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTTCTGCGGAAAGTCCATTACTCATTGACCGGTGTAAAAGGGGTTAGAGAGGCGTCGTCATTGTCAGAcagaag                                                            >  1:1101323/1‑150 (MQ=255)
                                                                                       cGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGCGGCGTCTTCATTGTCAGAcagcag                                                            >  2:124032/1‑150 (MQ=255)
                                                                                       cGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGAaagcag                                                            >  2:1201069/1‑150 (MQ=255)
                                                                                       cGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCGCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGAcagcag                                                            <  2:1640772/150‑1 (MQ=255)
                                                                                        gggAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGa                                                           <  2:80573/150‑1 (MQ=255)
                                                                                         ggAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGaa                                                          >  2:1024259/1‑150 (MQ=255)
                                                                                             tgtTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTg                                                      <  1:1537234/150‑1 (MQ=255)
                                                                                             tgtTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTg                                                      <  1:1530090/150‑1 (MQ=255)
                                                                                              gtTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTTGTTGGCCTGCTTAACGAAGGCTTAGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGATAGGCGTCTTCAATTACAGACATCAGAATTTGt                                                     >  2:1357305/1‑150 (MQ=255)
                                                                                              gtTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTTTCAGACAGCAGAATTTGt                                                     >  2:382820/1‑150 (MQ=255)
                                                                                               ttCTGAAAGGGCAGTGGGGCGATAAAACTGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTa                                                    <  2:1241140/150‑1 (MQ=255)
                                                                                               ttCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGCAAGTCCATTACTCATTGTCCGGTGTAATAGGGGTTAGAGAGGCGTCTTCAGTGTCAGACAGCAGAATTTGtt                                                    >  2:912017/1‑149 (MQ=255)
                                                                                               ttCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTa                                                    <  2:1605987/150‑1 (MQ=255)
                                                                                               ttCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTa                                                    <  1:565321/150‑1 (MQ=255)
                                                                                                tCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTGCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTAc                                                   <  2:402831/150‑1 (MQ=255)
                                                                                                  tGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCTAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGAAcgc                                                 >  2:1041221/1‑150 (MQ=255)
                                                                                                   gAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTAcgcg                                                >  2:1733130/1‑150 (MQ=255)
                                                                                                     aaGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCt                                              <  1:46889/150‑1 (MQ=255)
                                                                                                         gCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTc                                          <  2:564357/150‑1 (MQ=255)
                                                                                                                    aTAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTa                               >  1:1007364/1‑150 (MQ=255)
                                                                                                                            gCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGcc                       <  2:420939/150‑1 (MQ=255)
                                                                                                                             cTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCt                      >  1:758081/1‑150 (MQ=255)
                                                                                                                             cTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCt                      >  2:585444/1‑150 (MQ=255)
                                                                                                                               cAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGTAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGGCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGc                    <  1:964128/150‑1 (MQ=255)
                                                                                                                               cAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGCCAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGc                    >  2:452697/1‑150 (MQ=255)
                                                                                                                                  cgcCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGcccc                 >  1:1367679/1‑150 (MQ=255)
                                                                                                                                  cgcCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGcccc                 <  2:177792/150‑1 (MQ=255)
                                                                                                                                   gcCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGGTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCt                <  2:1605577/150‑1 (MQ=255)
                                                                                                                                    ccTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTg               >  1:685509/1‑150 (MQ=255)
                                                                                                                                    ccTGGTTGGCCTGCTTAACGAAGGCATCGAGTTGCTGCTGAAAGTCCATTAATCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCATg               <  2:864892/150‑1 (MQ=255)
                                                                                                                                      tGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGAc             >  2:1047209/1‑150 (MQ=255)
                                                                                                                                       ggTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACg            >  2:1750227/1‑150 (MQ=255)
                                                                                                                                        gTTGGCCTGCTTAAAGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCTGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGt           <  1:700206/150‑1 (MQ=255)
                                                                                                                                         ttGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTg          <  2:909316/150‑1 (MQ=255)
                                                                                                                                         gaagCCTGCGTTAAGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTg          >  2:98400/4‑150 (MQ=255)
                                                                                                                                           ggCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGcc        >  2:1113074/1‑150 (MQ=255)
                                                                                                                                            gCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCa       >  1:241025/1‑150 (MQ=255)
                                                                                                                                                gCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTg   >  1:1449815/1‑150 (MQ=255)
                                                                                                                                                 cTTACCGCAGGCGTCGAGTTGCTGCGGAAAGGCCATTACTCATTGTCCGGCGTAAAAGGGGTTAGAGAGGCGTCATCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGc  <  1:546601/150‑1 (MQ=255)
                                                                                                                                                 cTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGc  <  1:853681/150‑1 (MQ=255)
                                                                                                                                                 cTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGGCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGc  <  2:963340/150‑1 (MQ=255)
                                                                                                                                                 cTTAACGCAGGCTTCGAGGTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGc  <  2:1634127/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGC  >  NC_000913/440921‑441215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑