BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,880,374	A→T	5.6%	L283Q (CTG→CAG) ‡	recF ←	gap repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,880,374	0	A	T	5.6%	411.7 / 11.3	144	L283Q (CTG→CAG) ‡	recF	gap repair protein
Reads supporting (aligned to +/- strand): ref base A (76/60); new base T (2/6); total (78/66)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.01e-01

Only 100 of 144 total aligned reads displayed.

ATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAG  >  NC_000913/3880227‑3880522

aTCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGa                                                                                                                                                    >  1:449650/1‑150 (MQ=255)

 tCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTACGCGGCTGGCAAGCAGCCCGCGACGCTCTTCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACTCCGCGGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGAc                                                                                                                                                   <  1:889828/150‑1 (MQ=255)

 tCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGAc                                                                                                                                                   <  2:185537/150‑1 (MQ=255)

  cgcgCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACg                                                                                                                                                  >  2:145549/1‑150 (MQ=255)

    cgcTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTa                                                                                                                                                >  1:461446/1‑150 (MQ=255)

       tGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGTTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAgg                                                                                                                                             <  1:798335/150‑1 (MQ=255)

       tGACAAAGACCTGTGAGTGCGGCGCTTTTAAGCGGCGGGCAAGCAGCCCGCGACGCGCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAgg                                                                                                                                             <  2:1570044/150‑1 (MQ=255)

        gACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgc                                                                                                                                            >  2:1712927/1‑150 (MQ=255)

          cAAAGCCCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGCGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCGTTCACGGGTGCGGACCTCTCCTTGCGCCAGACGTACGgcgc                                                                                                                                          >  1:71991/1‑150 (MQ=255)

           aaaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGca                                                                                                                                         <  2:43405/150‑1 (MQ=255)

            aaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGcac                                                                                                                                        >  2:826548/1‑150 (MQ=255)

             aGACCTGTGATTGCGTCGCTTTTGAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCTTACGTCTGGCGcaag                                                                                                                                       <  1:1764093/150‑3 (MQ=255)

             aGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCTTACGTCTGGCGcaag                                                                                                                                       <  1:1700518/150‑3 (MQ=255)

             aGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCTTACGTCTGGCGcaag                                                                                                                                       <  1:1770720/150‑3 (MQ=255)

             aGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGcaca                                                                                                                                       >  2:790672/1‑150 (MQ=255)

              gACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCTTACGTCTGGCGcaagg                                                                                                                                      <  1:1316477/150‑4 (MQ=255)

              gACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACAt                                                                                                                                      <  1:49910/150‑1 (MQ=255)

              gACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACAt                                                                                                                                      <  2:1222504/150‑1 (MQ=255)

                 cTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCTTACGTCTGGCGcaaggaga                                                                                                                                   <  1:662673/150‑7 (MQ=255)

                  tgtgATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAAc                                                                                                                                  <  1:370618/150‑1 (MQ=255)

                  tgggATTGCGTCGCCTATAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCCTCTCTCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAAc                                                                                                                                  <  2:292646/147‑1 (MQ=255)

                    tgATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCGTTCAGGGGTGAGGCACTCTCCTTGCGCCAGACGTAAGGCGCACATCACCAg                                                                                                                                >  1:249665/1‑150 (MQ=255)

                       ttGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCtt                                                                                                                             <  2:1070135/150‑1 (MQ=255)

                       ttGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCtt                                                                                                                             >  2:632133/1‑150 (MQ=255)

                         gCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCCCATCAACAGCTTaa                                                                                                                           >  2:1648037/1‑150 (MQ=255)

                           gTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCCCATCAACAGCTTAAGc                                                                                                                         >  1:547068/1‑150 (MQ=255)

                            tCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCt                                                                                                                        >  1:603028/1‑150 (MQ=255)

                             cGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTg                                                                                                                       <  1:937814/150‑1 (MQ=255)

                             cGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTg                                                                                                                       >  1:601284/1‑150 (MQ=255)

                              gCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGc                                                                                                                      >  1:812204/1‑150 (MQ=255)

                               cTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCGATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCTCATCAACAGCTTTAGCTGcc                                                                                                                     >  2:330899/1‑150 (MQ=255)

                               cTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATATATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGAAAGGCGCACATAAACAGCTTAAGCTGcc                                                                                                                     >  2:336537/1‑150 (MQ=255)

                                   tAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGCCGTAAGGCGCACATCAACAGCTTAAGCTGCCCAcg                                                                                                                 >  1:1566194/1‑150 (MQ=255)

                                   tAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCAcg                                                                                                                 >  1:1499315/1‑150 (MQ=255)

                                     aGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCAcgcg                                                                                                               >  1:159619/1‑150 (MQ=255)

                                      gCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGa                                                                                                              <  2:1347847/150‑1 (MQ=255)

                                           tGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAgg                                                                                                         <  2:960298/150‑1 (MQ=255)

                                             gCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTa                                                                                                       >  2:1472697/1‑150 (MQ=255)

                                              cAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTAt                                                                                                      >  1:976343/1‑150 (MQ=255)

                                                 gcagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCATCAGCTGCCCACGCGATAAGGTATCtt                                                                                                   >  2:283697/1‑150 (MQ=255)

                                                   agcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTcc                                                                                                 >  1:1205475/1‑150 (MQ=255)

                                                    gcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTAACAGGTAGAGACACCGCCGCCCGCTTTCCCCGGTGAGGAACTCTCCTGGCGCCAGACGTACGGCTCACATCAACAGCTTAAGCTGACCACGCGAAAAGGAATCTACCa                                                                                                >  1:1780273/1‑150 (MQ=255)

                                                        gcgACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCgg                                                                                            >  2:958477/1‑150 (MQ=255)

                                                                catcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGt                                                                                    >  2:1426904/1‑150 (MQ=255)

                                                                       aaGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGcgcg                                                                             <  2:149483/150‑1 (MQ=255)

                                                                       aaGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGcgcg                                                                             <  2:1093294/150‑1 (MQ=255)

                                                                       aaGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGGGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGcgcg                                                                             <  1:1076373/150‑1 (MQ=255)

                                                                             agagGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCGCATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGGCGGCGCGAATGCg                                                                       <  1:259104/150‑1 (MQ=255)

                                                                               agGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTa                                                                     <  1:1462673/150‑1 (MQ=255)

                                                                                ggCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTaa                                                                    >  1:562795/1‑150 (MQ=255)

                                                                                  cAAAATCATCTAACAGGTAGAGACACCGCCGCAGGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCTCAACAACAGCTTAAGCTGCCCACGGGATAAGGTATCTTCCACCGGCGCACCGTAGGCGGGACAGCGAAAGt                                                                  >  2:903770/1‑150 (MQ=255)

                                                                                        catcTATCAGGTAGAGACCCCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATACGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGGGGAAGTCCGCtt                                                            >  1:147845/1‑150 (MQ=255)

                                                                                          tcTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtg                                                          >  1:1560124/1‑150 (MQ=255)

                                                                                          tcTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCGTtg                                                          >  1:1703165/1‑150 (MQ=255)

                                                                                           cTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgt                                                         >  1:402550/1‑150 (MQ=255)

                                                                                                aGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCggg                                                    <  1:10190/150‑1 (MQ=255)

                                                                                                aGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCggg                                                    <  1:980604/150‑1 (MQ=255)

                                                                                                 ggTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGc                                                   >  1:861126/1‑150 (MQ=255)

                                                                                                   tAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCg                                                 >  2:1079964/1‑150 (MQ=255)

                                                                                                       gaCACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTgcg                                             >  1:1057410/1‑150 (MQ=255)

                                                                                                       gaCACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTgcg                                             >  1:1561769/1‑150 (MQ=255)

                                                                                                         cacCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACTGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGcgcg                                           >  1:717045/1‑150 (MQ=255)

                                                                                                         cacCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGcgcg                                           >  1:1563720/1‑150 (MQ=255)

                                                                                                         cacCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGAACATAAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCAACCGGCCCACCGTCGGCGCGAATGAGTAAGTCCGCTTTGTGCGGGCCGTCcgcg                                           >  1:1490569/1‑150 (MQ=255)

                                                                                                          acCGCCGCCCGCGTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCGTAAGCTGCCCACGCGATAAGGGAGCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCgg                                          <  2:1247566/150‑1 (MQ=255)

                                                                                                           ccgccgCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGgt                                         >  1:611025/1‑150 (MQ=255)

                                                                                                           ccgccgCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGAGAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGgt                                         >  1:273755/1‑150 (MQ=255)

                                                                                                            cgccgcCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGgtg                                        <  1:790672/150‑1 (MQ=255)

                                                                                                            cgccgcCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGgtg                                        <  1:1438355/150‑1 (MQ=255)

                                                                                                            cgccgcCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGgtg                                        <  2:128514/150‑1 (MQ=255)

                                                                                                              ccgcccgcTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTa                                      <  1:632133/150‑1 (MQ=255)

                                                                                                                   cgcTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTa                                 <  2:1733605/150‑1 (MQ=255)

                                                                                                                     cTTTCCCGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGc                               <  1:1814470/150‑1 (MQ=255)

                                                                                                                      tttCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCt                              <  2:601284/150‑1 (MQ=255)

                                                                                                                       ttCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTg                             <  1:1131265/150‑1 (MQ=255)

                                                                                                                        tCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTgg                            <  2:1753216/150‑1 (MQ=255)

                                                                                                                           cGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGa                         >  1:10866/1‑150 (MQ=255)

                                                                                                                           cGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCATTGTGCGGGCCGTGCGCGGTGTAGGGTAGCTGGCGa                         >  1:305476/1‑150 (MQ=255)

                                                                                                                            gggTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACTGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGAt                        <  2:717045/150‑1 (MQ=255)

                                                                                                                             ggTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATc                       <  2:257140/150‑1 (MQ=255)

                                                                                                                              gTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATcg                      <  2:244992/150‑1 (MQ=255)

                                                                                                                               tGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATcgc                     <  2:726966/150‑1 (MQ=255)

                                                                                                                                gcggAACTCTCCATGCGCCAGACGGACGGCGCACAACAACAGCATAAGCAGCCCCCGCGATAAGGAATCTTCCACCGGCGCACCGTCGGCGCGCATGCGTAAGTCCGCTTCGTGCGGGCCGGGCGCGGTGTAGGTTAGCTGGCGATcgcg                    <  2:861940/148‑1 (MQ=255)

                                                                                                                                gAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATagcg                    >  2:823565/1‑150 (MQ=255)

                                                                                                                                gAGGAACTCTCCTTGCGCCAGACGGAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGCTAAGGTATCTTCCACCGGCGCACCGTCGGCGCTAATGCGTAAGTCCGCGTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATcgcg                    <  2:878807/150‑1 (MQ=255)

                                                                                                                                 aGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                   >  2:435432/1‑150 (MQ=255)

                                                                                                                                 aGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                   <  1:163812/150‑1 (MQ=255)

                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCGACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGtt                  >  2:1807334/1‑150 (MQ=255)

                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATTGCGtt                  >  2:347877/1‑150 (MQ=255)

                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGtt                  <  2:923903/150‑1 (MQ=255)

                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGtt                  >  2:1463178/1‑150 (MQ=255)

                                                                                                                                   gAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTc                 <  1:787033/150‑1 (MQ=255)

                                                                                                                                     aCTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTCAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaa               <  1:291834/150‑1 (MQ=255)

                                                                                                                                      ctctCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaaa              <  1:1570294/150‑1 (MQ=255)

                                                                                                                                       tctcCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaaaa             >  1:1529851/1‑150 (MQ=255)

                                                                                                                                       tctcCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaaaa             >  1:576567/1‑150 (MQ=255)

                                                                                                                                        ctcCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGCTCGCGGTCAAAAt            >  2:1394708/1‑150 (MQ=255)

                                                                                                                                          atgtGCGCCTTACGTCTGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTCTAGGTTAGCTGGCGATCGCGTTCAAAATTa          >  1:179096/4‑150 (MQ=255)

                                                                                                                                           cTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTAc         >  1:1530300/1‑150 (MQ=255)

                                                                                                                                                  cAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAg  >  2:1765909/1‑150 (MQ=255)

ATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAG  >  NC_000913/3880227‑3880522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`