Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,947,332 A→T 6.4% A212A (GCT→GCA hdfR ← flhDC operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,947,3320AT 6.4% 271.7 / 6.9 94A212A (GCT→GCAhdfRflhDC operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (32/55);  new base T (3/3);  total (36/58)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-01

Only 100 of 105 total aligned reads displayed.

TATCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAATA  >  NC_000913/3947184‑3947481
                                                                                                                                                    |                                                                                                                                                     
tATCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcag                                                                                                                                                      <  1:609304/150‑1 (MQ=255)
tATCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcag                                                                                                                                                      <  1:1569232/150‑1 (MQ=255)
 aTCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcagc                                                                                                                                                     <  2:39981/150‑1 (MQ=255)
 aTCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGGGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcagc                                                                                                                                                     <  2:1201152/150‑1 (MQ=255)
  tCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGGGTTGTGCTATCGACACCGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGGAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcagca                                                                                                                                                    <  1:1024913/150‑1 (MQ=255)
    gCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGa                                                                                                                                                  >  1:1203719/1‑150 (MQ=255)
    gCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGa                                                                                                                                                  >  2:1571657/1‑150 (MQ=255)
     cTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGaa                                                                                                                                                 >  2:217695/1‑150 (MQ=255)
        ttttGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTg                                                                                                                                              <  2:1509485/150‑1 (MQ=255)
         tttGCAGCCATATGGCATACAGCGTCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTgg                                                                                                                                             <  1:60468/150‑1 (MQ=255)
           tGCAGCCCTATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGtt                                                                                                                                           >  1:1714838/1‑150 (MQ=255)
           tGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCTGCTGAACTggca                                                                                                                                           <  2:1353803/150‑3 (MQ=255)
            gCAGCCATATGGCATAAAGTGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCTGCTGAACTggcac                                                                                                                                          <  1:803749/150‑4 (MQ=255)
            gCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTg                                                                                                                                          <  2:1453938/150‑1 (MQ=255)
             cAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCTGCTGAACTggcaca                                                                                                                                         <  1:1078655/150‑5 (MQ=255)
               gCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCa                                                                                                                                       <  2:1373187/150‑1 (MQ=255)
                   tatGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTTTGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAAt                                                                                                                                   <  2:1166823/150‑1 (MQ=255)
                   tatGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAAt                                                                                                                                   <  1:1354502/150‑1 (MQ=255)
                     tGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCTAAAATTTAGCATCGCAGTCGGTTTCTGTTCCAGTTCAGCAAAGATGGTTGTCAGAATgg                                                                                                                                 >  2:212522/1‑150 (MQ=255)
                     tGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATgg                                                                                                                                 <  1:1559312/150‑1 (MQ=255)
                       gCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGc                                                                                                                               <  1:1243137/150‑1 (MQ=255)
                         aTAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCAc                                                                                                                             >  1:381158/1‑150 (MQ=255)
                         aTAAAGCGGCCGTGAAAGGGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCAc                                                                                                                             <  2:442267/150‑1 (MQ=255)
                             aGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCTGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCg                                                                                                                         >  2:1134554/1‑150 (MQ=255)
                               cGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTc                                                                                                                       <  2:1114981/150‑1 (MQ=255)
                                    gTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCAc                                                                                                                  >  2:1529556/1‑150 (MQ=255)
                                    gTGAAAGTGTTGTGCTATCCACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCAc                                                                                                                  >  1:1820092/1‑150 (MQ=255)
                                     tGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGACCAGCTGACGGGTAGCAAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAAATAGTTGTCAGAATGTGCACTTCGTCAGCAcc                                                                                                                 >  1:217043/1‑150 (MQ=255)
                                       aaaGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGa                                                                                                               >  2:1263729/1‑150 (MQ=255)
                                        aaGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGAt                                                                                                              >  1:56547/1‑150 (MQ=255)
                                         aGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCTGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATc                                                                                                             <  2:325662/150‑1 (MQ=255)
                                          gtgtTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCa                                                                                                            <  2:1654630/150‑1 (MQ=255)
                                          gtgtTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCa                                                                                                            >  1:702083/1‑150 (MQ=255)
                                                tgCTATCGACAACGGTATGCAGGACGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTg                                                                                                      <  2:949308/150‑1 (MQ=255)
                                                  cTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGcc                                                                                                    <  2:381158/150‑1 (MQ=255)
                                                  cTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGcc                                                                                                    <  2:35126/150‑1 (MQ=255)
                                                    aTCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTc                                                                                                  <  1:1429250/150‑1 (MQ=255)
                                                          aaCGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGTGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGGTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTg                                                                                            <  2:909989/150‑1 (MQ=255)
                                                          aaCGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTg                                                                                            <  2:521998/150‑1 (MQ=255)
                                                           aCGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGt                                                                                           <  1:1378154/150‑1 (MQ=255)
                                                            cGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGTCGGGGAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTggt                                                                                          <  1:464803/150‑3 (MQ=255)
                                                                aTGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGaaa                                                                                      <  2:1729738/150‑1 (MQ=255)
                                                                 tGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCGGCCTCATGCTGTTGaaaa                                                                                     >  2:1715416/1‑150 (MQ=255)
                                                                     ggCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTg                                                                                 <  1:802687/150‑1 (MQ=255)
                                                                      gccgccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTgg                                                                                <  1:1386895/150‑1 (MQ=255)
                                                                        cgccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGcc                                                                              >  2:820515/1‑150 (MQ=255)
                                                                         gccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGccc                                                                             <  2:579394/150‑1 (MQ=255)
                                                                                ttACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCa                                                                      <  2:381935/150‑1 (MQ=255)
                                                                                ttACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGACAGTTCAGCAGCCCTGGGTGTCAGAATGGGCCCTTCGTCCGCACCGATCAAACCTGCCTCATGCGGTTGAAAATCTGGCCGCCACTCa                                                                      >  2:534419/1‑150 (MQ=255)
                                                                                 tACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGcaccccgacaa                                                                     >  1:781010/1‑144 (MQ=255)
                                                                                  aCGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAg                                                                    >  2:1749622/1‑150 (MQ=255)
                                                                                   cgcgCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGt                                                                   >  2:1109596/1‑150 (MQ=255)
                                                                                     ggcCCAGCTGACGGGTAGCCAGGTTCAACAATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCATCACCGATCTAACCTGCCTGATGTTGTTGAAAATCTGGCCCCCACTCAAGTCg                                                                 <  1:367031/149‑1 (MQ=255)
                                                                                      gcCCAGCTGACGGGTAGCCAGGTGGAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGc                                                                <  2:437906/150‑1 (MQ=255)
                                                                                          aGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGAt                                                            <  2:212640/150‑1 (MQ=255)
                                                                                           gCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATa                                                           >  2:949824/1‑150 (MQ=255)
                                                                                              gACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCCCTCAAGTCGCAGATAAtt                                                        >  2:1432627/1‑150 (MQ=255)
                                                                                              gACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAAtt                                                        >  2:1425456/1‑150 (MQ=255)
                                                                                               aCGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTa                                                       <  1:1250224/150‑1 (MQ=255)
                                                                                                cGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTaa                                                      <  2:422471/150‑1 (MQ=255)
                                                                                                cGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTaa                                                      <  2:503581/150‑1 (MQ=255)
                                                                                                 gggTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAg                                                     <  1:191556/150‑1 (MQ=255)
                                                                                                        cAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTccc                                              <  2:379666/150‑1 (MQ=255)
                                                                                                         aGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTTAGATCTCCCt                                             <  1:1169145/150‑1 (MQ=255)
                                                                                                         aGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCt                                             <  1:1571657/150‑1 (MQ=255)
                                                                                                          ggTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGGCCCCACTGAAGTCGCAGATAATTAAGATCTCCCtt                                            >  2:1184242/1‑150 (MQ=255)
                                                                                                           gTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCttt                                           <  2:655748/150‑1 (MQ=255)
                                                                                                              cAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGt                                        >  2:786706/1‑150 (MQ=255)
                                                                                                                aCCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGttt                                      <  2:268560/150‑1 (MQ=255)
                                                                                                                   aTTAATCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAATATCTGTCACCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGa                                   <  1:1128442/150‑1 (MQ=255)
                                                                                                                     tAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGAAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAg                                 <  2:1203719/150‑1 (MQ=255)
                                                                                                                      aaGCATCGCAAGCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAgg                                <  2:205463/150‑1 (MQ=255)
                                                                                                                        gCATCGCAATCTGTTGCTGTGCCAGTTCCGCAGACCTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATATGGCCCCCACTCAAGTCGCAGCTAATTAAGATCTCCCTTTAGTTTTGAAtggg                              >  1:67544/1‑150 (MQ=255)
                                                                                                                        gCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAgggg                              <  2:1484889/150‑1 (MQ=255)
                                                                                                                        gCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAgggg                              <  1:217695/150‑1 (MQ=255)
                                                                                                                          aTCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCa                            >  2:637698/1‑150 (MQ=255)
                                                                                                                          aTCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCa                            >  2:746413/1‑150 (MQ=255)
                                                                                                                            cGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCCGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACt                          <  1:248724/150‑1 (MQ=255)
                                                                                                                              cAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGTCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTATGATCTCCCTTTAGTTTTGAAGGGGCACTgg                        <  1:869433/150‑1 (MQ=255)
                                                                                                                               aaTCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGt                       <  2:1431515/150‑1 (MQ=255)
                                                                                                                                  gtGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGtata                    <  2:826764/149‑1 (MQ=255)
                                                                                                                                      tGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgc                <  1:1623434/150‑1 (MQ=255)
                                                                                                                                      tGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGAACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgc                >  2:678809/1‑150 (MQ=255)
                                                                                                                                        cTGTTCCAGTTCAGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCAACGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgcgc              <  2:1811970/150‑1 (MQ=255)
                                                                                                                                        cTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgcgc              <  1:4807/150‑1 (MQ=255)
                                                                                                                                        cTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgcgc              <  1:1529556/150‑1 (MQ=255)
                                                                                                                                          gtgCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTa            <  2:8296/150‑1 (MQ=255)
                                                                                                                                          caaccAGTTCTGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTa            >  2:617771/4‑150 (MQ=255)
                                                                                                                                          caaccAGTTCTGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTa            >  2:1296472/4‑150 (MQ=255)
                                                                                                                                            gCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTaaa          >  2:37936/1‑150 (MQ=255)
                                                                                                                                            gCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGACCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTaaa          >  1:39289/1‑150 (MQ=255)
                                                                                                                                            accAGTTCTGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTaaa          >  2:420394/2‑150 (MQ=255)
                                                                                                                                             ccAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAg         >  1:1015763/1‑150 (MQ=255)
                                                                                                                                               aGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTg       <  2:1820092/150‑1 (MQ=255)
                                                                                                                                                   gagcggAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTGATGCTTTTGAAAATCTGGGCCCCACGCAAGTCGGAGATAATTTAGATCTACGTTTAGTTTTGAATGGGGACGGGTATTAAGCGCTAAAGTGAAat   >  2:1497035/2‑150 (MQ=255)
                                                                                                                                                   cagcagAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTTGTTTGAAGGGGGAACTGGATTAACCCGATAAAGTGaata   >  1:1679814/1‑148 (MQ=255)
                                                                                                                                                    agcagAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAata  <  1:1807639/150‑1 (MQ=255)
                                                                                                                                                    agcagAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAata  >  1:1656189/1‑150 (MQ=255)
                                                                                                                                                    agcagAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATATCCCTTTAGTTTTGAAGGGGAACTGGTATAAAGCGCTAAAGTGAAata  >  2:1627337/1‑150 (MQ=255)
                                                                                                                                                    agcagAACAGGTAGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCCCTCAAGTCGCAGATAATTATGAAATCCCTTTAGTTTTGAAGGGGCACTCGTATAACGCGCTAAAGTGAAata  >  2:272905/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TATCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAATA  >  NC_000913/3947184‑3947481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: