Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,980,726 G→A 5.7% intergenic (+30/‑161) wecG → / → yifK UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase/putative APC family amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,980,7260GA 5.7% 389.4 / 7.6 122intergenic (+30/‑161)wecG/yifKUDP‑N‑acetyl‑D‑mannosaminuronic acid transferase/putative APC family amino acid transporter
Reads supporting (aligned to +/- strand):  ref base G (51/64);  new base A (4/3);  total (55/67)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 125 total aligned reads displayed.

AAAATCTGGCAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACA  >  NC_000913/3980577‑3980874
                                                                                                                                                     |                                                                                                                                                    
aaaaTCTGGCAAACGCTGGGGCTGGTGTGGCTCTAGCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGTCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTgg                                                                                                                                                      <  1:869181/150‑1 (MQ=255)
 aaaTCTGGCAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGt                                                                                                                                                     <  1:927203/150‑1 (MQ=255)
  aaTCTGGCAAAGGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTc                                                                                                                                                    <  1:420503/150‑1 (MQ=255)
  aaTCTGGCAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTc                                                                                                                                                    <  1:1785127/150‑1 (MQ=255)
  aaTCTGGCAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTc                                                                                                                                                    >  2:214635/1‑150 (MQ=255)
    tCTGGCAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCAc                                                                                                                                                  <  1:923044/150‑1 (MQ=255)
         cAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGt                                                                                                                                             >  1:1531811/1‑150 (MQ=255)
           aaCGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCTCTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGACCACTCCGTCg                                                                                                                                           <  1:1821406/150‑1 (MQ=255)
           aaCGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCg                                                                                                                                           <  2:740812/150‑1 (MQ=255)
           aaCGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCg                                                                                                                                           <  2:1654788/150‑1 (MQ=255)
           aaCGCTGGGGCTGGAGTGGCTCTACCGCCTGCTGTCGCAGCCGAGCCGCAGTAAGCGGCAGCTTCGTTTGCTGCGGTATTTACGCTGGCACTACACCGGCAACCTATGCTGTTCCTCTCGTTCTACAGCGACGGAGTGGTCACTCCGTcc                                                                                                                                           >  1:97650/1‑149 (MQ=255)
           aaCGCTGGGGCGGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTGAATTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGACCACTCCGTCg                                                                                                                                           <  1:263207/150‑1 (MQ=255)
               cTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTg                                                                                                                                       <  1:214635/150‑1 (MQ=255)
                 ggggCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTc                                                                                                                                     <  1:1262283/150‑1 (MQ=255)
                  gggCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGACCACTCCGTCGctttaca                                                                                                                                    <  1:678113/150‑7 (MQ=255)
                   ggCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTg                                                                                                                                   >  2:1127750/1‑150 (MQ=255)
                     cTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCt                                                                                                                                 <  2:1670023/150‑1 (MQ=255)
                       ggAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCttt                                                                                                                               >  2:622284/1‑150 (MQ=255)
                       ggAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCGATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCttt                                                                                                                               <  2:1410211/150‑1 (MQ=255)
                         gggggCTCAACCGACTGCTTTGGCAGCCGTGCCGCTTTAAGTGTCAGCTTCGTTTGCGGCGTTATATACGCTGTCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTCAAGCGACGGTGTGGTCACTCCGTCGCCTGTCTGCttttt                                                                                                                             <  2:307103/147‑1 (MQ=255)
                         aGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGGCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCttttt                                                                                                                             <  1:890395/150‑1 (MQ=255)
                           tGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCTTTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCTCTACTCCGGCATCCTATGTTTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGGCTTTCTGCTTTTtta                                                                                                                           <  1:941444/150‑1 (MQ=255)
                            ggCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTttat                                                                                                                          <  2:675975/150‑1 (MQ=255)
                             gCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATAATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTttatt                                                                                                                         >  1:660426/1‑150 (MQ=255)
                             gCTATACCGCCTGCTTTCGCAGCCGAGCCGAATTAAGCGTAAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCCGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTttatt                                                                                                                         <  2:1604167/150‑1 (MQ=255)
                                  aCCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTAcaca                                                                                                                    <  1:809546/150‑1 (MQ=255)
                                  aCCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTAcaca                                                                                                                    >  1:1356276/1‑150 (MQ=255)
                                  aCCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTAcaca                                                                                                                    <  2:522614/150‑1 (MQ=255)
                                       cTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCa                                                                                                               <  1:1464990/150‑1 (MQ=255)
                                       cTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCAATACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCa                                                                                                               <  2:1560336/150‑1 (MQ=255)
                                              cGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAAt                                                                                                        >  1:423981/1‑150 (MQ=255)
                                                 agccgagccgCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAAtttt                                                                                                     <  2:1240405/150‑1 (MQ=255)
                                                  gccgagccgCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAAttttt                                                                                                    <  2:1090134/150‑1 (MQ=255)
                                                     gagccgCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAAt                                                                                                 <  2:520572/150‑1 (MQ=255)
                                                       gccgCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGc                                                                                               >  1:202578/1‑150 (MQ=255)
                                                       gccgCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGAATTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGc                                                                                               <  1:457515/150‑1 (MQ=255)
                                                         cgCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCtt                                                                                             >  2:1249951/1‑150 (MQ=255)
                                                          gCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCttt                                                                                            <  1:1365626/150‑1 (MQ=255)
                                                           cATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTAAAAATTTGAATGCtttg                                                                                           <  2:1459953/150‑2 (MQ=255)
                                                              tAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTAttt                                                                                        <  1:140399/150‑1 (MQ=255)
                                                               aaGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTg                                                                                       >  1:1201539/1‑150 (MQ=255)
                                                                aGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGc                                                                                      >  1:1443693/1‑150 (MQ=255)
                                                                aGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGc                                                                                      >  1:12538/1‑150 (MQ=255)
                                                                 gCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACGTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTAAAAATTTGAATGCtttgtgtaat                                                                                     <  2:1601710/150‑8 (MQ=255)
                                                                 gCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGcc                                                                                     <  2:791815/150‑1 (MQ=255)
                                                                       gCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtct                                                                               >  1:60171/1‑150 (MQ=255)
                                                                        cTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtctc                                                                              >  2:593131/1‑150 (MQ=255)
                                                                         ttCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTcc                                                                             <  2:450810/150‑1 (MQ=255)
                                                                             tttGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGaa                                                                         <  2:502282/150‑1 (MQ=255)
                                                                              ttGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAAt                                                                        <  1:1533026/150‑1 (MQ=255)
                                                                               tgctgcGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAAtt                                                                       >  1:1197420/1‑150 (MQ=255)
                                                                                gctgcGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTa                                                                      <  2:165632/150‑1 (MQ=255)
                                                                                gctgcGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTa                                                                      <  2:1191329/150‑1 (MQ=255)
                                                                                  tgcGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACg                                                                    >  1:38513/1‑150 (MQ=255)
                                                                                      ttATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGaaaa                                                                <  2:1804007/150‑1 (MQ=255)
                                                                                       tATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAAc                                                               <  1:1758833/150‑1 (MQ=255)
                                                                                             cGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGc                                                         <  1:382192/150‑1 (MQ=255)
                                                                                                tGGCACTACACCGGCAACCGATGATTTTCCTCTCTTTGTAACGCGACGGAGAGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCTATCAAATTTTTAATGCTTTAGTTGCCATTTCTCCTGAATTACGAAAACATTTGCacc                                                      >  2:343122/1‑148 (MQ=255)
                                                                                                   cACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACt                                                   >  2:1136019/1‑150 (MQ=255)
                                                                                                   cACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACt                                                   <  2:1471750/150‑1 (MQ=255)
                                                                                                    aCTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTc                                                  <  1:310757/150‑1 (MQ=255)
                                                                                                     cTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCg                                                 <  2:157407/150‑1 (MQ=255)
                                                                                                       acacCGGCAACCTATGCTTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATATGCAACACTCGAt                                               >  1:878981/1‑150 (MQ=255)
                                                                                                         acCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGt                                             <  2:1495401/150‑1 (MQ=255)
                                                                                                          ccGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTa                                            >  2:1239169/1‑150 (MQ=255)
                                                                                                          ccGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTa                                            >  2:1824232/1‑150 (MQ=255)
                                                                                                            ggCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTAcc                                          >  1:1357413/1‑150 (MQ=255)
                                                                                                            ggCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTAcc                                          <  2:1653540/150‑1 (MQ=255)
                                                                                                            ggCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTAcc                                          <  2:1531811/150‑1 (MQ=255)
                                                                                                             gCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTAccc                                         <  1:1293123/150‑1 (MQ=255)
                                                                                                                aCCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATa                                      >  1:172537/1‑150 (MQ=255)
                                                                                                                 ccTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATaa                                     >  2:884046/1‑150 (MQ=255)
                                                                                                                 ccTATGATTTTCCACTCTTTGAAAAGCGACGGAGTGGTCACTCCTTCTCATTTATGCTTTTTTATTACACAAAGCATACAAATTTTTAATGCTTTATTTGCCATTTCTCCTTAAATACAAAAACATTTGCAAAACTCGATGTACCCATaa                                     >  1:358684/1‑150 (MQ=255)
                                                                                                                    aTGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGa                                  <  1:1523170/150‑1 (MQ=255)
                                                                                                                          ttCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCg                            <  1:1326975/150‑1 (MQ=255)
                                                                                                                          ttCCTATCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCg                            <  2:176877/150‑1 (MQ=255)
                                                                                                                           tCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCgg                           >  1:1141562/1‑150 (MQ=255)
                                                                                                                            ccTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGt                          <  2:1356276/150‑1 (MQ=255)
                                                                                                                             ctctctTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTa                         <  1:513213/150‑1 (MQ=255)
                                                                                                                               ctctTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTTAATTACGAAAACATTTGCAAAACTCGATGTACCCATAACGATAACCGGTAac                       >  2:112134/1‑150 (MQ=255)
                                                                                                                               ctctTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAac                       >  2:617587/1‑150 (MQ=255)
                                                                                                                                tctTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAaca                      >  2:152634/1‑150 (MQ=255)
                                                                                                                                   ttGTAAAGCGACGGAGTGGTCTCTCCGTCGCCTGTCTGCTTTTTAATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCg                   <  2:573841/150‑1 (MQ=255)
                                                                                                                                   ttGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCg                   >  1:458504/1‑150 (MQ=255)
                                                                                                                                    tGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCgg                  >  2:431196/1‑150 (MQ=255)
                                                                                                                                    agacaggCGACGGAGTGACCACTCCGTCGCTTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCgg                  >  1:1740080/7‑150 (MQ=255)
                                                                                                                                       aaaGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa               >  2:463118/1‑150 (MQ=255)
                                                                                                                                        aaGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAg              <  2:799737/150‑1 (MQ=255)
                                                                                                                                        aaGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAg              >  2:56232/1‑150 (MQ=255)
                                                                                                                                         ggCGACGGAGTGACCACTCCGTCGCTTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGc             >  1:1484459/2‑150 (MQ=255)
                                                                                                                                         aGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGc             >  1:555070/1‑150 (MQ=255)
                                                                                                                                         aGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGc             <  1:826129/150‑1 (MQ=255)
                                                                                                                                           cGACGGAGTGACCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTAACCATAACGATAACCGGTAACACCGGAAAGCAt           >  1:1008636/1‑150 (MQ=255)
                                                                                                                                            gACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATg          <  1:1249951/150‑1 (MQ=255)
                                                                                                                                              cGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCa        >  2:256547/1‑150 (MQ=255)
                                                                                                                                               ggAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCaa       <  2:1813972/150‑1 (MQ=255)
                                                                                                                                               ggAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTAAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCaa       >  2:149866/1‑150 (MQ=255)
                                                                                                                                                 tgttgTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTTCGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAaac     <  1:153716/146‑1 (MQ=255)
                                                                                                                                                 aGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAaac     <  1:574126/150‑1 (MQ=255)
                                                                                                                                                    ggTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAaacaca  >  1:496802/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAAATCTGGCAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACA  >  NC_000913/3980577‑3980874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: