Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,088,091 G→C 9.0% intergenic (+34/+16) yiiG → / ← frvR DUF3829 family lipoprotein/putative frv operon regulator; contains a PTS EIIA domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,088,0910GC 9.0% 345.3 / 22.4 111intergenic (+34/+16)yiiG/frvRDUF3829 family lipoprotein/putative frv operon regulator; contains a PTS EIIA domain
Reads supporting (aligned to +/- strand):  ref base G (56/45);  new base C (6/4);  total (62/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

Only 100 of 115 total aligned reads displayed.

ACTGGGATAAAGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCG  >  NC_000913/4087942‑4088239
                                                                                                                                                     |                                                                                                                                                    
aCTGGGATAAAGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATgcgg                                                                                                                                                      <  1:703203/150‑1 (MQ=255)
     gATAAAGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCAt                                                                                                                                                 >  1:879282/1‑150 (MQ=255)
         aaGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGa                                                                                                                                             >  2:238913/1‑150 (MQ=255)
          agagCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGac                                                                                                                                            >  1:1275908/1‑150 (MQ=255)
            agCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGCCGCATCCGacac                                                                                                                                          <  2:465498/150‑1 (MQ=255)
            agCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGGTGCTGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGCCGCAGCCGacac                                                                                                                                          <  1:1259205/150‑1 (MQ=255)
              cAACTTCAGGACGCAACATCACGCTGGATGGTCGAAGACTCTTTCCCGATAGCATTAGGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGCCGCATCCGacactg                                                                                                                                        <  1:1737320/150‑3 (MQ=255)
               aaCTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCTAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGt                                                                                                                                       <  2:1644392/150‑1 (MQ=255)
                aCTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGtt                                                                                                                                      >  1:706248/1‑150 (MQ=255)
                 cTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTc                                                                                                                                     >  1:1458041/1‑150 (MQ=255)
                  ttCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCCACCCAGttaa                                                                                                                                    >  2:1529371/1‑148 (MQ=255)
                     aGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATACGACACAGTTAAccc                                                                                                                                 >  2:1540866/1‑150 (MQ=255)
                       gACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCt                                                                                                                               <  2:90981/150‑1 (MQ=255)
                        aCGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCtt                                                                                                                              <  1:1769210/150‑1 (MQ=255)
                         cGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCttt                                                                                                                             >  2:1102304/1‑149 (MQ=255)
                         cGCAAACTCAACCTGGATGGTCGAAGACTCGTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGCATGCGGCGCATCCGACACAGTTCACCCCTtg                                                                                                                             <  1:55614/150‑1 (MQ=255)
                         cGCAAACTAAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCTAGTACAACGAAATGGTTTATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTtg                                                                                                                             <  2:1595858/150‑1 (MQ=255)
                          gCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTtgt                                                                                                                            <  2:422899/150‑1 (MQ=255)
                           cAAACTCAAGCTGGATGGTCGAAGACTCTTTCCAGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGGTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTAACAACTtgtt                                                                                                                           >  1:127074/1‑149 (MQ=255)
                           cAAACTCAAGCTGGATGGTCGAAGACTCTTTCCAGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGATAAACGTTTTTGATGATAAATGCAGTGTCGGATGCTGCGCATCCGACACAGTTCACCCCTtttg                                                                                                                           >  1:107041/1‑150 (MQ=255)
                           cAAACTCAAGCTGGATGGGCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTtgtg                                                                                                                           <  2:766816/150‑1 (MQ=255)
                            aaaCTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGa                                                                                                                          >  2:676171/1‑150 (MQ=255)
                            aaaCTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGa                                                                                                                          <  1:1093583/150‑1 (MQ=255)
                            aaaCTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGa                                                                                                                          <  1:806140/150‑1 (MQ=255)
                             aaCTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGaa                                                                                                                         >  2:202161/1‑150 (MQ=255)
                              aCTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGAATATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATTAGGCGCATCCGAAACACTTCACCCATTTTGaat                                                                                                                        >  2:929760/1‑149 (MQ=255)
                               cTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGt                                                                                                                       <  2:1526285/150‑1 (MQ=255)
                                 cAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTATACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTaa                                                                                                                     >  2:989018/1‑150 (MQ=255)
                                   aGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGGCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACt                                                                                                                   >  1:344391/1‑150 (MQ=255)
                                   aGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCTGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACt                                                                                                                   <  2:1389546/150‑1 (MQ=255)
                                   aGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACt                                                                                                                   <  2:1729578/150‑1 (MQ=255)
                                   aGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACt                                                                                                                   <  2:706248/150‑1 (MQ=255)
                                    gCTGCATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTc                                                                                                                  <  1:1823901/150‑1 (MQ=255)
                                       ggATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGGGGCGAATACGAAACAATTCACCCCTTGTGAATGAACTGCaa                                                                                                               >  1:1527596/1‑149 (MQ=255)
                                        tatgttggAAGACTCTTTCCGGAGAGCATTACGAGAGTACAAAGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAATAGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATa                                                                                                              <  1:61076/143‑1 (MQ=255)
                                          tGGTCGAAGACTCTTTCCCGAGAGCAATACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATaaa                                                                                                            >  2:1119020/1‑150 (MQ=255)
                                            gTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATg                                                                                                          <  2:1319996/150‑1 (MQ=255)
                                             tCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGc                                                                                                         >  2:712746/1‑150 (MQ=255)
                                              cGAAGACTCTTTCCCTTGAGCATTACGCGATTACAACTAAATGGTTGATGACTATAATAGCGTGCGTTAAACGTTTTTGATGATAAATGCAGTTTAGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGcc                                                                                                        <  1:1378067/150‑1 (MQ=255)
                                               gAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACCCAGTTCACCCCTTGGGAAGTAACTCCATAAATGCCt                                                                                                       >  2:903322/1‑150 (MQ=255)
                                               gAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCt                                                                                                       >  1:436882/1‑150 (MQ=255)
                                               gAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTAACCCCTTGTGAAGTAACTCCATAAATGCCt                                                                                                       >  1:1192940/1‑150 (MQ=255)
                                               gAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGACCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCa                                                                                                     <  2:1747751/150‑1 (MQ=255)
                                                 aGACTCTTTCCCGAGAGCATTACGCGAGTACAACGATATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCa                                                                                                     <  1:1048624/150‑1 (MQ=255)
                                                  gACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCat                                                                                                    <  2:662676/150‑1 (MQ=255)
                                                     tctTTCCCGAGAGCATTACGTGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATc                                                                                                 >  2:602935/1‑150 (MQ=255)
                                                      ctTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATcc                                                                                                >  2:53062/1‑150 (MQ=255)
                                                         tCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAATTGCTGTGTCTGATGCGGCGCATCCGACACAGTTCACGCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGt                                                                                             <  1:727895/150‑1 (MQ=255)
                                                            cGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa                                                                                          >  2:1277370/1‑150 (MQ=255)
                                                            cGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGATTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa                                                                                          >  2:1296117/1‑150 (MQ=255)
                                                             gagaGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAc                                                                                         <  1:882484/150‑1 (MQ=255)
                                                                agCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATACGACACAGTTAACCCCATGTGAAGTAACTCCATAAAAGCCTCATATCCTGTAAACACg                                                                                      >  1:1787837/1‑150 (MQ=255)
                                                                 gCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCgg                                                                                     <  1:108791/150‑1 (MQ=255)
                                                                  cATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGc                                                                                    <  1:238913/150‑1 (MQ=255)
                                                                   aTTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCa                                                                                   >  1:999543/1‑150 (MQ=255)
                                                                     tACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTATCCCGGCaat                                                                                 <  1:1119020/150‑1 (MQ=255)
                                                                         cgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAAAGCCTCATATCCTGTTAACCCGGCaataata                                                                             >  2:611828/1‑149 (MQ=255)
                                                                           aGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtctc                                                                           >  2:252300/1‑150 (MQ=255)
                                                                           aGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTAGGATTCGGCGCATCCGACACAGTTAACCCCTTGTGAAGTAACTACATAAATGCCTAATATCCTGTTTAACCGGCAATAAtcta                                                                           >  1:606532/1‑149 (MQ=255)
                                                                              aCAACGAAATGGTAGATGACTATAATAGCCAGCGTATAACGTTTTTGATTATAAATGCAGTGTCGGATGCGGCGCTTCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAATTGCCTCATATCCTGTTAACCCGGCAATAATCTccgc                                                                        <  1:314326/150‑1 (MQ=255)
                                                                                aaCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgccg                                                                      <  2:1642785/150‑1 (MQ=255)
                                                                                 aCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCgg                                                                     >  2:1233873/1‑150 (MQ=255)
                                                                                 aCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTAGGATGCGGCGCATAAGACACAGTTAAACCCAATTGAAGTAAAAACATAAAATCCTCATATAAAGTAAAAACCGCAATAAtctacacacg                                                                     >  1:1722869/1‑143 (MQ=255)
                                                                                       tGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTg                                                               <  2:1002130/150‑1 (MQ=255)
                                                                                       tGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTg                                                               <  1:399457/150‑1 (MQ=255)
                                                                                          ttGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATCCTGaca                                                            >  1:3202/1‑150 (MQ=255)
                                                                                               gaCTCTAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAata                                                       <  1:90507/150‑1 (MQ=255)
                                                                                               gaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGCCACAGTTCACCCCTTGTGAAGTAACTACATAAATGACTCATATACAGTTAACCCGGCAATAATCTACGCCGGATGCTGACACAata                                                       >  1:1426203/1‑150 (MQ=255)
                                                                                               gaCTAAAATAGCCTGCGTTAAACGTTTTAGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAata                                                       <  1:659507/150‑1 (MQ=255)
                                                                                                aCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAatat                                                      >  1:521826/1‑150 (MQ=255)
                                                                                                 cTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATg                                                     >  2:144042/1‑150 (MQ=255)
                                                                                                 cTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATg                                                     >  1:765194/1‑150 (MQ=255)
                                                                                                    taataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGCCACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCtt                                                  >  1:417002/1‑150 (MQ=255)
                                                                                                     aataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTg                                                 >  2:978628/1‑150 (MQ=255)
                                                                                                         gCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAg                                             <  2:1735050/150‑1 (MQ=255)
                                                                                                         gCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAg                                             <  2:1309301/150‑1 (MQ=255)
                                                                                                           cTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGAt                                           <  2:1458041/150‑1 (MQ=255)
                                                                                                            tGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATa                                          <  1:438481/150‑1 (MQ=255)
                                                                                                             gCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAg                                         <  2:344391/150‑1 (MQ=255)
                                                                                                              cGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCCCCCCTTGTGAAGTATCTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAAATCCGCCGGATGCTGACACAATATGCCTGCCAGATAgg                                        >  2:1109606/1‑149 (MQ=255)
                                                                                                                  aaaCGTTTTTGATGATAAATGCAGTGTCGTATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTaaa                                    <  1:1579206/150‑1 (MQ=255)
                                                                                                                          ttGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTa                            <  2:449376/150‑1 (MQ=255)
                                                                                                                          ttGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTa                            >  1:1099687/1‑150 (MQ=255)
                                                                                                                          ttGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTa                            <  2:1070483/150‑1 (MQ=255)
                                                                                                                          ttGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTa                            <  1:53062/150‑1 (MQ=255)
                                                                                                                             atgatAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCt                         >  1:1711614/1‑150 (MQ=255)
                                                                                                                             atgaaAAATGCAGTGTCGGATGCGGCGCATCCGCCACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCt                         >  1:1090658/1‑150 (MQ=255)
                                                                                                                                   aaTGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGAc                   >  1:935118/1‑150 (MQ=255)
                                                                                                                                      gCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGCcgcg                >  2:427756/1‑150 (MQ=255)
                                                                                                                                       ctgtgtCGGATGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACgcgc               >  2:1237265/3‑150 (MQ=255)
                                                                                                                                           gtCGGATGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcg           >  1:871509/1‑150 (MQ=255)
                                                                                                                                           gtCGGATGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcg           >  1:1265057/1‑150 (MQ=255)
                                                                                                                                           gtCGGATGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCGGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcg           >  1:1175580/1‑150 (MQ=255)
                                                                                                                                            tCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATTCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcgg          >  1:1300226/1‑150 (MQ=255)
                                                                                                                                             cGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcggc         >  2:1694410/1‑150 (MQ=255)
                                                                                                                                              ggATGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAt                                                                   <  1:461569/91‑1 (MQ=255)
                                                                                                                                              ggATGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAt                                                                   >  2:461569/1‑91 (MQ=255)
                                                                                                                                                 tGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcggcggcg     >  2:1051147/1‑150 (MQ=255)
                                                                                                                                                    ggcgCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGgcggcggcg  >  2:975550/1‑150 (MQ=255)
                                                                                                                                                    ggcgCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGgcggcggcg  <  1:1540866/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACTGGGATAAAGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCG  >  NC_000913/4087942‑4088239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: