Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,132,430 T→A 7.8% intergenic (+163/‑186) metL → / → metF Bifunctional aspartokinase/homoserine dehydrogenase 2/5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,132,4300TA 7.8% 355.3 / 14.1 123intergenic (+163/‑186)metL/metFBifunctional aspartokinase/homoserine dehydrogenase 2/5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (45/68);  new base A (6/4);  total (51/72)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 131 total aligned reads displayed.

TAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAA  >  NC_000913/4132282‑4132578
                                                                                                                                                    |                                                                                                                                                    
taatGCCCTGTACTCATGTTTTAGGGTTTATGTTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGtttgctttg                                                                                                                                                     <  1:340355/150‑1 (MQ=255)
  atGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCa                                                                                                                                                   <  2:506634/150‑1 (MQ=255)
    gCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATa                                                                                                                                                 >  2:309879/1‑150 (MQ=255)
     cccGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATAc                                                                                                                                                >  1:179063/1‑150 (MQ=255)
       cGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGTCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCg                                                                                                                                              <  1:505686/150‑1 (MQ=255)
       cGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCg                                                                                                                                              <  2:566847/150‑1 (MQ=255)
       cGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCg                                                                                                                                              >  2:1628525/1‑150 (MQ=255)
        ggTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTATGCAAAGCAAACCgg                                                                                                                                             <  1:492523/150‑1 (MQ=255)
          tACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAt                                                                                                                                           <  1:1604350/150‑1 (MQ=255)
          tACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTATGCAAAGCAAACCGGac                                                                                                                                           <  1:1347079/150‑2 (MQ=255)
           aCTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAtt                                                                                                                                          <  1:632283/150‑1 (MQ=255)
           aCTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTATGCAAAGCAAACCGGact                                                                                                                                          <  2:1489841/150‑3 (MQ=255)
            cTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAttt                                                                                                                                         >  2:1028072/1‑150 (MQ=255)
             tCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAtttt                                                                                                                                        <  1:1515152/150‑1 (MQ=255)
               aTGTTTTCTGGTTTATGGTTTCTAATGAAATATATTGATTTATCCTAGGATTAGGGCGGATTTAGCGTTTACGACGTATCCGGCAAGAAGCACTAAGTACATGGTTTGCTTATATTTGCTGTCCGGTTTGCTTTGCATACCGGTttttct                                                                                                                                      <  2:1654237/150‑1 (MQ=255)
               aTGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAttttct                                                                                                                                      <  2:1555050/150‑1 (MQ=255)
               aTGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAttttct                                                                                                                                      <  2:1697849/150‑1 (MQ=255)
                    ttCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAttttctttttc                                                                                                                                 <  1:1333196/150‑1 (MQ=255)
                     tCGGGTTTATGGTTTCTTATGAAATATATTGTTTTATGATTGGATTAGGCCGGATTAATCGTTTACGACGAATCCGGCAAGTAGCAATAAGTACATGGTTAGTTTTTATTTGCAGTCCGGTTTGCTTTGCATACCGGAttttctttttct                                                                                                                                <  2:1175938/150‑1 (MQ=255)
                      cGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTCCGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATtttctttttctt                                                                                                                               <  1:491261/150‑1 (MQ=255)
                       gggTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTa                                                                                                                              <  2:570698/150‑1 (MQ=255)
                        ggTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGATGCAATAAGTCCATGGTTAGTTGATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTaa                                                                                                                             >  2:1060465/1‑149 (MQ=255)
                               ggTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACAACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTg                                                                                                                      >  2:757831/1‑150 (MQ=255)
                                     tAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGttt                                                                                                                >  2:36077/1‑150 (MQ=255)
                                     tAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGttt                                                                                                                >  2:750818/1‑150 (MQ=255)
                                      aaTGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGtttt                                                                                                               >  2:1800859/1‑150 (MQ=255)
                                       aTGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGttttt                                                                                                              <  1:658961/150‑1 (MQ=255)
                                         gAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTc                                                                                                            >  1:737444/1‑150 (MQ=255)
                                            atatatTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACTAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATATGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACAATCCTGAAGTTTTTTCATc                                                                                                         >  1:243822/1‑150 (MQ=255)
                                              atatTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCATGAAGCATTAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTATCTTATTCTTACCATCCTGAAGTTTTTTCATCtt                                                                                                       >  1:334902/1‑150 (MQ=255)
                                                atTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTcc                                                                                                     >  1:1494316/1‑150 (MQ=255)
                                                atTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTcc                                                                                                     >  2:85497/1‑150 (MQ=255)
                                                 ttGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTccc                                                                                                    <  1:548050/150‑1 (MQ=255)
                                                   gAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTg                                                                                                  >  1:108918/1‑150 (MQ=255)
                                                    aaTTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGa                                                                                                 <  2:1172396/150‑1 (MQ=255)
                                                         tCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGAttttt                                                                                            <  2:900410/150‑1 (MQ=255)
                                                           aTAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTcc                                                                                          >  1:126492/1‑150 (MQ=255)
                                                                      ccGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCAtt                                                                               >  2:1123425/1‑150 (MQ=255)
                                                                       cGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTg                                                                              <  2:1277068/150‑1 (MQ=255)
                                                                         gATTTAGCGTTTACGACGAATCCAGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGATTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGt                                                                            <  1:1379233/150‑1 (MQ=255)
                                                                         gATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGt                                                                            <  2:1620592/150‑1 (MQ=255)
                                                                          aTTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTc                                                                           <  1:66284/150‑1 (MQ=255)
                                                                                 gTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTc                                                                    <  2:1550332/150‑1 (MQ=255)
                                                                                  tttACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCg                                                                   >  1:898182/1‑150 (MQ=255)
                                                                                   ttACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCgg                                                                  <  2:676723/150‑1 (MQ=255)
                                                                                   ttACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCgg                                                                  >  2:1435974/1‑150 (MQ=255)
                                                                                    tACGACGTATACGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGt                                                                 <  2:484845/150‑1 (MQ=255)
                                                                                    tACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGt                                                                 <  2:850730/150‑1 (MQ=255)
                                                                                    tACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGt                                                                 >  2:495163/1‑150 (MQ=255)
                                                                                     acgacgAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGtt                                                                <  1:1757309/150‑1 (MQ=255)
                                                                                         cgaATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACg                                                            <  1:889790/150‑1 (MQ=255)
                                                                                           aaTCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCAACTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGcc                                                          >  2:764254/1‑150 (MQ=255)
                                                                                            aTCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGccc                                                         <  1:1535197/150‑1 (MQ=255)
                                                                                              ccGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCtt                                                       >  2:902522/1‑150 (MQ=255)
                                                                                              ccGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCtt                                                       >  2:1178280/1‑150 (MQ=255)
                                                                                               cGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTc                                                      <  2:835081/150‑1 (MQ=255)
                                                                                               cGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTc                                                      >  1:1281554/1‑150 (MQ=255)
                                                                                               cGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTGTTCGGTTGACGCCCTTc                                                      >  2:1242389/1‑150 (MQ=255)
                                                                                               cGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTATCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTc                                                      >  2:1116634/1‑150 (MQ=255)
                                                                                                ggCAAGAAGCAATAAGTACCTGGTTAGGTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCg                                                     <  2:1279874/150‑1 (MQ=255)
                                                                                                  cAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGc                                                   <  1:554341/150‑1 (MQ=255)
                                                                                                       agCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGTCTTTTc                                              >  1:1441077/1‑150 (MQ=255)
                                                                                                        gCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTcc                                             <  1:1285554/150‑1 (MQ=255)
                                                                                                        gCAATAAGTACATGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATACTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTGATTTTTCGGTTGACGCCCTTAGGCTTTTCCt                                            >  1:1499367/1‑150 (MQ=255)
                                                                                                         cAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCt                                            >  2:1107118/1‑150 (MQ=255)
                                                                                                         cAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCt                                            <  2:584879/150‑1 (MQ=255)
                                                                                                         cAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCt                                            <  1:1339746/150‑1 (MQ=255)
                                                                                                           aTAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTc                                          <  1:1028072/150‑1 (MQ=255)
                                                                                                           aTAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTc                                          <  1:380732/150‑1 (MQ=255)
                                                                                                            tAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCa                                         <  2:323003/150‑1 (MQ=255)
                                                                                                            tAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCa                                         <  1:1214033/150‑1 (MQ=255)
                                                                                                            tAAGTACATGGTTAGTTTATATTTGCAGTCCGGATTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCa                                         <  1:241270/150‑1 (MQ=255)
                                                                                                             aaGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCAt                                        <  2:1826697/150‑1 (MQ=255)
                                                                                                                tACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCtt                                     <  1:610092/150‑1 (MQ=255)
                                                                                                                tACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCtt                                     <  2:179063/150‑1 (MQ=255)
                                                                                                                   aTGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTAc                                  <  1:1628525/150‑1 (MQ=255)
                                                                                                                   aTGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTAc                                  <  1:1232045/150‑1 (MQ=255)
                                                                                                                   aTGGTTAGGTTATATTTGCAGTCCGGGTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTAc                                  <  2:1678205/150‑1 (MQ=255)
                                                                                                                      gTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATc                               <  2:1322509/150‑1 (MQ=255)
                                                                                                                        tAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTg                             <  1:674858/150‑1 (MQ=255)
                                                                                                                        tAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTATTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTg                             <  1:252017/150‑1 (MQ=255)
                                                                                                                         aGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTgg                            <  1:85497/150‑1 (MQ=255)
                                                                                                                         aGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTgg                            <  1:1604829/150‑1 (MQ=255)
                                                                                                                          gTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACAATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGa                           >  1:1046299/1‑150 (MQ=255)
                                                                                                                          gTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAGTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATATTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCGTCATCTTTACATCTGGa                           >  1:55735/1‑150 (MQ=255)
                                                                                                                            ttATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACg                         <  1:1590230/150‑1 (MQ=255)
                                                                                                                              atatTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTc                       <  1:1201456/150‑1 (MQ=255)
                                                                                                                                  agaaaatCCGGTATGCAAAGCAAACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTaaa                   >  2:690399/7‑150 (MQ=255)
                                                                                                                                    gCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACg                 <  1:452478/150‑1 (MQ=255)
                                                                                                                                     cAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACgg                <  2:1094633/150‑1 (MQ=255)
                                                                                                                                     aaatCCGGTATGCAAAGCAAACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACgg                >  1:1789020/4‑150 (MQ=255)
                                                                                                                                         ccGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGTTTTTTCCTCACCATCATTGGTCATTTTTAGTTTGACGACCTTAGGCTTTTCCTTCATCTTTACATATGTACTTATAAACGGATaa            >  1:361315/1‑149 (MQ=255)
                                                                                                                                         ccGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAg            >  2:569557/1‑150 (MQ=255)
                                                                                                                                          cGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCATTCGGATTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGa           >  1:213469/1‑150 (MQ=255)
                                                                                                                                            gTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGAtg         <  2:963251/150‑1 (MQ=255)
                                                                                                                                               tgctttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGc      <  2:773014/150‑1 (MQ=255)
                                                                                                                                                gctttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGca     <  1:999977/150‑1 (MQ=255)
                                                                                                                                                gctttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAAAGGATAGATGTGca     >  1:1144275/1‑150 (MQ=255)
                                                                                                                                                 ctttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCACTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGcac    >  1:671990/1‑150 (MQ=255)
                                                                                                                                                   ttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGcacaa  >  1:312165/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAA  >  NC_000913/4132282‑4132578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: