Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,136,033 G→A 6.6% intergenic (+18/‑75) katG → / → yijE catalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,136,0330GA 6.6% 373.5 / 9.3 122intergenic (+18/‑75)katG/yijEcatalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein
Reads supporting (aligned to +/- strand):  ref base G (56/56);  new base A (4/4);  total (61/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.33e-01

Only 100 of 134 total aligned reads displayed.

TTGGTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGC  >  NC_000913/4135885‑4136182
                                                                                                                                                    |                                                                                                                                                     
ttGGTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGgc                                                                                                                                                      >  1:1430533/1‑150 (MQ=255)
ttGGTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGgc                                                                                                                                                      <  1:1609692/150‑1 (MQ=255)
 tGGTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTTGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGgct                                                                                                                                                     >  1:769189/1‑150 (MQ=255)
 tGGTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGgct                                                                                                                                                     >  1:1634944/1‑150 (MQ=255)
 tGGTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTGTTAAAGACTTCGAGGCGGCATGGGTGAAATTGATGAACCTCGAACGTTTCGACCTGATGTAATCTGACCCCGTTCAGCGgctg                                                                                                                                                    >  2:457310/1‑150 (MQ=255)
  ggTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGgctg                                                                                                                                                    <  2:1556623/150‑1 (MQ=255)
  ggTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGgctg                                                                                                                                                    <  1:341112/150‑1 (MQ=255)
    ttCTAAGTCGGTCGTGCGTGCGGAGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGgctgct                                                                                                                                                  <  1:1647095/150‑1 (MQ=255)
    ttCTAACTCCGTCCTGCGTGCGGGGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCTTGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGgctgct                                                                                                                                                  <  2:1541143/150‑1 (MQ=255)
       tAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCtgcttgc                                                                                                                                               >  2:469211/1‑150 (MQ=255)
       tAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCtgcttgc                                                                                                                                               >  2:352782/1‑150 (MQ=255)
         aCTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTg                                                                                                                                             <  1:1625032/150‑1 (MQ=255)
         aCTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGGGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCtgcttgccg                                                                                                                                             <  1:498624/150‑3 (MQ=255)
         aCTCCGTCCTGCGTGCGGTGCCGGAAGTTTACGCCAGTAGCGATTCCCACGACAAGTTTGTTAAAGACTTCGTCGCGGCATGGGTGAAAGTGATGAACCTCGACAGTTTCGAACTGCTGTAATCTGACACCGTTAAGAGGCTGCTTGCTg                                                                                                                                             >  1:1704360/1‑150 (MQ=255)
          cTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTgg                                                                                                                                            <  2:979990/150‑1 (MQ=255)
          cTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTgg                                                                                                                                            >  1:27948/1‑150 (MQ=255)
           tCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCGGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGc                                                                                                                                           <  1:110388/150‑1 (MQ=255)
            ccGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGCCCCTCGCCCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCa                                                                                                                                          >  1:1290535/1‑150 (MQ=255)
                ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGCCCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                      >  2:169126/1‑150 (MQ=255)
                ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                      >  1:454976/1‑150 (MQ=255)
                ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                      >  2:626500/1‑150 (MQ=255)
                 cTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGc                                                                                                                                     >  2:21620/1‑150 (MQ=255)
                  tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGTCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGc                                                                                                                                           <  1:277836/143‑1 (MQ=255)
                  tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGTCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGc                                                                                                                                           >  2:277836/1‑143 (MQ=255)
                  tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCt                                                                                                                                    <  1:1065436/150‑1 (MQ=255)
                  tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGCTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAAGCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCt                                                                                                                                    <  1:84909/150‑1 (MQ=255)
                    ggtgctGTGGCGGAAGTTTACGCCAGTAGCGATGCCCTCGAGAAGTTTGTTTAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGTCCTGCTGTAATATTACACCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGa                                                                                                                                  <  1:1281392/149‑1 (MQ=255)
                    cgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTTATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTGa                                                                                                                                  <  1:419988/150‑1 (MQ=255)
                      tgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAAc                                                                                                                                <  2:593196/150‑1 (MQ=255)
                         ggTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGtt                                                                                                                             >  2:923295/1‑150 (MQ=255)
                          gTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTGAACgggg                                                                                                                            <  1:1262820/150‑4 (MQ=39)
                          gTGGCGGAAGTTTACGCCAGTAGCGATGCCAACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGCCCGTTTCGACCTGCTGTAATCTGACCCCGTACAGCGCCTGCTTGCTGGCAGTCGCTCAACGtaa                                                                                                                            >  1:1131465/1‑148 (MQ=255)
                               ggAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTAc                                                                                                                       >  2:745743/1‑150 (MQ=255)
                                   gTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGc                                                                                                                   >  1:522076/1‑150 (MQ=255)
                                        cGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGtata                                                                                                              >  2:734503/1‑150 (MQ=255)
                                          ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                            >  1:752573/1‑150 (MQ=255)
                                          ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                            >  1:1587226/1‑150 (MQ=255)
                                            aGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATTAACCTCGACCGTTTCGACGTGCTGTAATCTGACCCCGTTAAGCGGCTGCTTGCTGGCAGTCGCTGAAGGTTCTTTTCAAGCGTATAGTgg                                                                                                          >  2:449714/1‑150 (MQ=255)
                                            aGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTgg                                                                                                          >  2:449712/1‑150 (MQ=255)
                                             gTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTGCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTggg                                                                                                         <  1:1106259/150‑1 (MQ=255)
                                              tAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAATCGCTGAACGTTCTTTACCAGCGTATAGTGGGc                                                                                                        >  1:292023/1‑150 (MQ=255)
                                                 cGATGCCCACGAGAAGGTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGGGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaa                                                                                                     <  1:886487/150‑1 (MQ=255)
                                                   aTGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaacg                                                                                                   <  2:175379/150‑1 (MQ=255)
                                                        cACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAAc                                                                                              >  2:1412421/1‑150 (MQ=255)
                                                         aCGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACt                                                                                             <  1:625457/150‑1 (MQ=255)
                                                            agaAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTaca                                                                                          >  2:1652776/1‑150 (MQ=255)
                                                             gaAGTTTGTTAAAGACTTCGTGGCGGCATGTGTGAAAGTCATGAACCTCGACCGTTTCTACCTGCTGTAATCTGCCCCCGTTCCGCGGCTGCTAGCTGGCAGTCGCTGAACGTTCTTTCACCGCGGATATTGAGCGAACGAAAACTacac                                                                                         >  1:280336/1‑150 (MQ=255)
                                                                    gTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCATCGTATAGTGGGCGAACGAAAACTACACACTGGAt                                                                                  >  1:53454/1‑150 (MQ=255)
                                                                     ttAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtc                                                                                 <  1:1072069/150‑1 (MQ=255)
                                                                      tAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGCCCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtct                                                                                >  2:304526/1‑150 (MQ=255)
                                                                      tAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTTCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtct                                                                                >  2:1393608/1‑150 (MQ=255)
                                                                        aaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGCCCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctct                                                                              >  1:1669836/1‑150 (MQ=255)
                                                                        aaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctct                                                                              <  1:623887/150‑1 (MQ=255)
                                                                          gACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCa                                                                            >  2:1689717/1‑150 (MQ=255)
                                                                          gACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCa                                                                            <  1:1405517/150‑1 (MQ=255)
                                                                            cTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATg                                                                          <  2:1611804/150‑1 (MQ=255)
                                                                            cTTCGTGGCGGCATGGGGGAAAGTGATGAACCTCGACCGGTTCGCCCTGATGTAAGCTGACCACGTGCATCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTCCCCGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATg                                                                          <  1:794551/150‑1 (MQ=255)
                                                                            attCGGGGCTGCATGGGTGAAAGTGATGAACCTCGACCGGGGCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGGATAGTGGGAGAACGAAAACTACACACTGGATCTCTCATg                                                                          <  1:787766/149‑1 (MQ=255)
                                                                              tCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTc                                                                        >  1:1398220/1‑150 (MQ=255)
                                                                               cGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGttt                                                                       >  2:1330683/1‑148 (MQ=255)
                                                                               cGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCt                                                                       >  2:496364/1‑150 (MQ=255)
                                                                                  ggcggcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGcc                                                                    >  2:846695/1‑150 (MQ=255)
                                                                                      gcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGATAACTACACACTGGATCTCTCATGTCTGCCGCAg                                                                <  1:997139/150‑1 (MQ=255)
                                                                                       cATGTGTGAAAGTGATGAACCTCGACCGTTTCTACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACTAAAACTACACACTGTATCTCTCATGTCTGCCGCAgg                                                               <  1:254959/150‑1 (MQ=255)
                                                                                          gggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGaaa                                                            >  2:393358/1‑150 (MQ=255)
                                                                                           ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAag                                                           <  1:950240/150‑1 (MQ=255)
                                                                                           ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAag                                                           <  1:1761128/150‑1 (MQ=255)
                                                                                           ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTATCATGTCTGCCGCAGGAAag                                                           >  2:338799/1‑150 (MQ=255)
                                                                                             tGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCCGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAagag                                                         <  1:465746/150‑1 (MQ=255)
                                                                                                aaGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCac                                                      >  1:354031/1‑149 (MQ=255)
                                                                                                 aGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAc                                                     <  1:21620/150‑1 (MQ=255)
                                                                                                 aGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACAATAGTATAGTGGTCGAACGATATCTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAc                                                     <  2:1381989/150‑1 (MQ=255)
                                                                                                  gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAct                                                    <  1:449712/150‑2 (MQ=255)
                                                                                                  gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAcc                                                    >  2:1283702/1‑150 (MQ=255)
                                                                                                  gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGGCTGCCGCAGGAAAGAGCAAcc                                                    <  2:898691/150‑1 (MQ=255)
                                                                                                      tgaACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACt                                                <  1:33820/150‑1 (MQ=255)
                                                                                                           cTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCaa                                           <  2:1059925/150‑1 (MQ=255)
                                                                                                              gACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGACCGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAATAGCAACCCACTGGCAATCa                                        >  1:1644723/1‑150 (MQ=255)
                                                                                                                       gACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTgg                               <  2:197017/150‑1 (MQ=255)
                                                                                                                       gACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTgg                               <  1:123205/150‑1 (MQ=255)
                                                                                                                        aCCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGt                              >  2:1296713/1‑150 (MQ=255)
                                                                                                                         ccTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGtt                             >  2:196403/1‑150 (MQ=255)
                                                                                                                           tgctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTTTAGTGGGCGAACGAAAACTACACCCTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgg                           >  1:1319769/1‑149 (MQ=255)
                                                                                                                            gctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgtg                          <  1:1796710/150‑1 (MQ=255)
                                                                                                                             ctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGc                         <  1:1607037/150‑1 (MQ=255)
                                                                                                                               gTAATCCGACCCCGTTCAGCGTCGGCGTGCTGGCAGTCGCTGAACGTTCTTTACCCGCGTATAGTGGGCGAACGAACACTCTACACTGGATCTCTCATGTCTGCCGCAGGAAAGCGCAACCCACTGGCAATCAGTGGCCTGGGTGTGCTc                       <  1:1091263/150‑1 (MQ=255)
                                                                                                                                    cTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACt                  <  2:27948/150‑1 (MQ=255)
                                                                                                                                    cTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACt                  <  2:18373/150‑1 (MQ=255)
                                                                                                                                    atGACCCCGTTCAGCGGCTGATTGCTTGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTGATGTCTGCCGCAGGAAAGAGCAACCCAGTGGCAATCAGTGGCCTGGTTGTGCTCACACt                  <  2:1704360/149‑1 (MQ=255)
                                                                                                                                       agaacGTTCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCAGACTTAt               >  2:174959/5‑150 (MQ=38)
                                                                                                                                          ccGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCTTGTCTGCCGCAGGAAAGAGCAACCCTCTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTg            <  1:188231/150‑1 (MQ=255)
                                                                                                                                             ttCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAg         <  1:600496/150‑1 (MQ=255)
                                                                                                                                             ttCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCGGCGTATAGTGGGCGAACGAAAACTACACCCTGGATCTCTCATGTCGGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCCCTCGTATCTGCAg         >  2:109546/1‑150 (MQ=255)
                                                                                                                                             ttCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAg         >  2:817494/1‑150 (MQ=255)
                                                                                                                                             ttCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATAAGTGGCCTGGTTGTGCTCACACTTATCTGGAg         >  2:738957/1‑150 (MQ=255)
                                                                                                                                              tCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGt        <  2:661090/150‑1 (MQ=255)
                                                                                                                                                aGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTta      >  1:707694/1‑150 (MQ=255)
                                                                                                                                                aGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTta      >  1:1791824/1‑150 (MQ=255)
                                                                                                                                                 gCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGTCCTGGTTGTGCTCACACTTATCTGGAGTtat     <  1:457310/150‑1 (MQ=255)
                                                                                                                                                    gctgctTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGc  <  1:1161508/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGGTTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGC  >  NC_000913/4135885‑4136182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: