Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,136,038 T→C 6.7% intergenic (+23/‑70) katG → / → yijE catalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,136,0380TC 6.7% 350.7 / 11.8 119intergenic (+23/‑70)katG/yijEcatalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein
Reads supporting (aligned to +/- strand):  ref base T (55/55);  new base C (4/4);  total (59/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

Only 100 of 130 total aligned reads displayed.

TTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGA  >  NC_000913/4135889‑4136186
                                                                                                                                                     |                                                                                                                                                    
ttCTAAGTCGGTCGTGCGTGCGGAGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGgctgct                                                                                                                                                      <  1:1647095/150‑1 (MQ=255)
ttCTAACTCCGTCCTGCGTGCGGGGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCTTGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGgctgct                                                                                                                                                      <  2:1541143/150‑1 (MQ=255)
   tAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCtgcttgc                                                                                                                                                   >  2:352782/1‑150 (MQ=255)
     aCTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTg                                                                                                                                                 <  1:1625032/150‑1 (MQ=255)
     aCTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGGGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCtgcttgccg                                                                                                                                                 <  1:498624/150‑3 (MQ=255)
     aCTCCGTCCTGCGTGCGGTGCCGGAAGTTTACGCCAGTAGCGATTCCCACGACAAGTTTGTTAAAGACTTCGTCGCGGCATGGGTGAAAGTGATGAACCTCGACAGTTTCGAACTGCTGTAATCTGACACCGTTAAGAGGCTGCTTGCTg                                                                                                                                                 >  1:1704360/1‑150 (MQ=255)
      cTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTgg                                                                                                                                                >  1:27948/1‑150 (MQ=255)
      cTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTgg                                                                                                                                                >  1:1381989/1‑150 (MQ=255)
        ccGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGCCCCTCGCCCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCa                                                                                                                                              >  1:1290535/1‑150 (MQ=255)
         cGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAg                                                                                                                                             >  1:18373/1‑150 (MQ=255)
            ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                          >  1:454976/1‑150 (MQ=255)
            ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                          >  2:511228/1‑150 (MQ=255)
             cTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGc                                                                                                                                         >  2:21620/1‑150 (MQ=255)
              tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGTCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGc                                                                                                                                               >  2:277836/1‑143 (MQ=255)
              tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCt                                                                                                                                        <  1:566077/150‑1 (MQ=255)
              tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCt                                                                                                                                        <  1:1065436/150‑1 (MQ=255)
              tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGCTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAAGCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCt                                                                                                                                        <  1:84909/150‑1 (MQ=255)
                  tgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAAc                                                                                                                                    <  2:593196/150‑1 (MQ=255)
                   gcgGTGGCGTAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCTACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACg                                                                                                                                   <  1:1094536/150‑1 (MQ=255)
                   gcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGACCCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACg                                                                                                                                   <  1:114041/150‑1 (MQ=255)
                   gcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACg                                                                                                                                   <  1:1500318/150‑1 (MQ=255)
                    cgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATCAACCTCGACCCTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGACGCTGAACGt                                                                                                                                  >  2:1694199/1‑150 (MQ=255)
                     ggTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGtt                                                                                                                                 >  2:923295/1‑150 (MQ=255)
                      gTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTGAACgggg                                                                                                                                <  1:1262820/150‑4 (MQ=39)
                      gTGGCGGAAGTTTACGCCAGTAGCGATGCCAACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGCCCGTTTCGACCTGCTGTAATCTGACCCCGTACAGCGCCTGCTTGCTGGCAGTCGCTCAACGtaa                                                                                                                                >  1:1131465/1‑148 (MQ=255)
                       tGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCt                                                                                                                               >  1:1611804/1‑150 (MQ=255)
                         gCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCttt                                                                                                                             <  2:1636605/150‑1 (MQ=255)
                          cGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTa                                                                                                                            >  2:708193/1‑150 (MQ=255)
                           ggAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTAc                                                                                                                           >  2:745743/1‑150 (MQ=255)
                               gTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGc                                                                                                                       >  1:522076/1‑150 (MQ=255)
                                    cGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGtata                                                                                                                  >  2:734503/1‑150 (MQ=255)
                                      ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                                >  1:752573/1‑150 (MQ=255)
                                      ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                                >  1:1725506/1‑150 (MQ=255)
                                      ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                                >  1:1587226/1‑150 (MQ=255)
                                        aGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATTAACCTCGACCGTTTCGACGTGCTGTAATCTGACCCCGTTAAGCGGCTGCTTGCTGGCAGTCGCTGAAGGTTCTTTTCAAGCGTATAGTgg                                                                                                              >  2:449714/1‑150 (MQ=255)
                                        aGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTgg                                                                                                              >  2:449712/1‑150 (MQ=255)
                                         gTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTGCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTggg                                                                                                             <  1:1106259/150‑1 (MQ=255)
                                          tAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAATCGCTGAACGTTCTTTACCAGCGTATAGTGGGc                                                                                                            >  1:292023/1‑150 (MQ=255)
                                               aTGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaacg                                                                                                       <  2:175379/150‑1 (MQ=255)
                                                tGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCCGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaacga                                                                                                      >  1:1266347/1‑150 (MQ=255)
                                                    cACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAAc                                                                                                  >  2:1412421/1‑150 (MQ=255)
                                                        agaAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTaca                                                                                              >  2:1652776/1‑150 (MQ=255)
                                                         gaAGTTTGTTAAAGACTTCGTGGCGGCATGTGTGAAAGTCATGAACCTCGACCGTTTCTACCTGCTGTAATCTGCCCCCGTTCCGCGGCTGCTAGCTGGCAGTCGCTGAACGTTCTTTCACCGCGGATATTGAGCGAACGAAAACTacac                                                                                             >  1:280336/1‑150 (MQ=255)
                                                                gTTAAAGACTTCGTGGCGGCAGGCGTGAAAGTGCTGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAt                                                                                      <  1:290283/150‑1 (MQ=255)
                                                                 ttAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtc                                                                                     <  1:1072069/150‑1 (MQ=255)
                                                                  tAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGCCCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtct                                                                                    >  2:304526/1‑150 (MQ=255)
                                                                   aaaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctc                                                                                   >  1:70221/1‑150 (MQ=255)
                                                                   aaaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGGTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctc                                                                                   <  1:80568/150‑1 (MQ=255)
                                                                    aaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGCCCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctct                                                                                  >  1:1669836/1‑150 (MQ=255)
                                                                    aaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctct                                                                                  <  1:623887/150‑1 (MQ=255)
                                                                      gACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCa                                                                                >  2:1689717/1‑150 (MQ=255)
                                                                      gACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCa                                                                                <  1:1405517/150‑1 (MQ=255)
                                                                        cTTCGTGGCGGCATGGGGGAAAGTGATGAACCTCGACCGGTTCGCCCTGATGTAAGCTGACCACGTGCATCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTCCCCGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATg                                                                              <  1:794551/150‑1 (MQ=255)
                                                                        attCGGGGCTGCATGGGTGAAAGTGATGAACCTCGACCGGGGCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGGATAGTGGGAGAACGAAAACTACACACTGGATCTCTCATg                                                                              <  1:787766/149‑1 (MQ=255)
                                                                           cGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGttt                                                                           >  2:1330683/1‑148 (MQ=255)
                                                                           cGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCt                                                                           >  2:496364/1‑150 (MQ=255)
                                                                                 ggcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCa                                                                     <  2:1421403/150‑1 (MQ=255)
                                                                                  gcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGATAACTACACACTGGATCTCTCATGTCTGCCGCAg                                                                    <  1:997139/150‑1 (MQ=255)
                                                                                   cATGTGTGAAAGTGATGAACCTCGACCGTTTCTACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACTAAAACTACACACTGTATCTCTCATGTCTGCCGCAgg                                                                   <  1:254959/150‑1 (MQ=255)
                                                                                   cATGGGTGAAAGTGATGAACATCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAgg                                                                   >  2:114659/1‑150 (MQ=255)
                                                                                     tGGGTGAAAGTGTTGAACCTCGACCGTTTGGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGATCGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGaa                                                                 <  2:769189/150‑1 (MQ=255)
                                                                                       ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAag                                                               <  1:950240/150‑1 (MQ=255)
                                                                                       ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTATCATGTCTGCCGCAGGAAag                                                               >  2:338799/1‑150 (MQ=255)
                                                                                        gTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGGCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAaga                                                              <  1:469211/150‑1 (MQ=255)
                                                                                         tGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCCGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAagag                                                             <  1:465746/150‑1 (MQ=255)
                                                                                           aaaGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTCCCAGCGTATAGTGGTCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCa                                                           <  1:351710/150‑1 (MQ=255)
                                                                                            aaGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCac                                                          >  1:354031/1‑149 (MQ=255)
                                                                                             aGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAc                                                         <  1:21620/150‑1 (MQ=255)
                                                                                             aGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACAATAGTATAGTGGTCGAACGATATCTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAc                                                         <  2:1381989/150‑1 (MQ=255)
                                                                                              ttgatgaACCTCGACCTTTTCTACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGTCAGTCGCATAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGTATCTCTCATGTCTGCCTCAGGAAAGAGCAAcc                                                        <  1:449714/149‑1 (MQ=255)
                                                                                              gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAct                                                        <  1:449712/150‑2 (MQ=255)
                                                                                              gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAcc                                                        >  2:1283702/1‑150 (MQ=255)
                                                                                              gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGGCTGCCGCAGGAAAGAGCAAcc                                                        <  2:898691/150‑1 (MQ=255)
                                                                                                  tgaACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACt                                                    <  1:33820/150‑1 (MQ=255)
                                                                                                       cTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCaa                                               <  2:1059925/150‑1 (MQ=255)
                                                                                                          gACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGACCGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAATAGCAACCCACTGGCAATCa                                            >  1:1644723/1‑150 (MQ=255)
                                                                                                           aCCGTGTCGACCTGATGTAAGCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGGTGAACGGGCATTACCAGCGGATAGTGGGCGAACGAAAACTAAACACAGGATCTCTAATGTATGACGCAGGAAAAAGCAACCCACGGGCAAGCaa                                           >  2:1369357/1‑149 (MQ=255)
                                                                                                                   gACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTgg                                   <  2:197017/150‑1 (MQ=255)
                                                                                                                   gACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTgg                                   <  1:123205/150‑1 (MQ=255)
                                                                                                                     ccTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGtt                                 >  2:196403/1‑150 (MQ=255)
                                                                                                                     ccTGCTGTAATCTGACCCCGTTCAGCGGCTGATTGCTGGCAGTCGCTGAACGTTATTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATATCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGAAATAAGTGGACTGGtt                                 >  1:25927/1‑150 (MQ=255)
                                                                                                                       tgctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTTTAGTGGGCGAACGAAAACTACACCCTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgg                               >  1:1319769/1‑149 (MQ=255)
                                                                                                                        gctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgtg                              <  1:1796710/150‑1 (MQ=255)
                                                                                                                         ctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGc                             >  2:357780/1‑150 (MQ=255)
                                                                                                                           gTAATCCGACCCCGTTCAGCGTCGGCGTGCTGGCAGTCGCTGAACGTTCTTTACCCGCGTATAGTGGGCGAACGAACACTCTACACTGGATCTCTCATGTCTGCCGCAGGAAAGCGCAACCCACTGGCAATCAGTGGCCTGGGTGTGCTc                           <  1:1091263/150‑1 (MQ=255)
                                                                                                                                cTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACt                      <  2:27948/150‑1 (MQ=255)
                                                                                                                                cTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACt                      <  2:18373/150‑1 (MQ=255)
                                                                                                                                atGACCCCGTTCAGCGGCTGATTGCTTGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTGATGTCTGCCGCAGGAAAGAGCAACCCAGTGGCAATCAGTGGCCTGGTTGTGCTCACACt                      <  2:1704360/149‑1 (MQ=255)
                                                                                                                                  gACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTa                    <  2:454976/150‑1 (MQ=255)
                                                                                                                                   agaacGTTCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCAGACTTAt                   >  2:174959/5‑150 (MQ=38)
                                                                                                                                    ccccGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCCATCAGTGGCCTGGTTGTGCTCACACTTATc                  >  2:918959/1‑150 (MQ=255)
                                                                                                                                         ttCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAg             <  1:600496/150‑1 (MQ=255)
                                                                                                                                         ttCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAg             >  2:817494/1‑150 (MQ=255)
                                                                                                                                          tCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGt            <  2:661090/150‑1 (MQ=255)
                                                                                                                                            aGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTta          >  1:1791824/1‑150 (MQ=255)
                                                                                                                                            aGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTta          >  1:707694/1‑150 (MQ=255)
                                                                                                                                                  tgcttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTg    <  1:1393608/150‑1 (MQ=255)
                                                                                                                                                  tgcttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTg    >  2:1299531/1‑150 (MQ=255)
                                                                                                                                                   gcttgctGGCAGTCGCTGTACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTgg   <  2:1131465/150‑1 (MQ=255)
                                                                                                                                                    cttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGa  <  2:691012/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTCTAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGA  >  NC_000913/4135889‑4136186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: