Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,136,039 T→A 6.6% intergenic (+24/‑69) katG → / → yijE catalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,136,0390TA 6.6% 350.2 / 6.6 120intergenic (+24/‑69)katG/yijEcatalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein
Reads supporting (aligned to +/- strand):  ref base T (54/57);  new base A (5/4);  total (59/61)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATT  >  NC_000913/4135892‑4136188
                                                                                                                                                   |                                                                                                                                                     
tAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCtgcttgc                                                                                                                                                     >  2:352782/1‑150 (MQ=255)
  aCTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTg                                                                                                                                                   <  1:1625032/150‑1 (MQ=255)
  aCTCCGTCCTGCGTGCGGTGCCGGAAGTTTACGCCAGTAGCGATTCCCACGACAAGTTTGTTAAAGACTTCGTCGCGGCATGGGTGAAAGTGATGAACCTCGACAGTTTCGAACTGCTGTAATCTGACACCGTTAAGAGGCTGCTTGCTg                                                                                                                                                   >  1:1704360/1‑150 (MQ=255)
   cTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTgg                                                                                                                                                  >  1:27948/1‑150 (MQ=255)
   cTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTgg                                                                                                                                                  >  1:1381989/1‑150 (MQ=255)
      cGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAg                                                                                                                                               >  1:18373/1‑150 (MQ=255)
         ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGCCCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                            >  2:169126/1‑150 (MQ=255)
         ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                            >  1:454976/1‑150 (MQ=255)
         ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                            >  2:511228/1‑150 (MQ=255)
          cTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGc                                                                                                                                           >  2:21620/1‑150 (MQ=255)
           tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGTCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGc                                                                                                                                                 <  1:277836/143‑1 (MQ=255)
           tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGTCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGc                                                                                                                                                 >  2:277836/1‑143 (MQ=255)
           tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCt                                                                                                                                          <  1:1065436/150‑1 (MQ=255)
           tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCt                                                                                                                                          <  1:566077/150‑1 (MQ=255)
           tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGCTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAAGCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCt                                                                                                                                          <  1:84909/150‑1 (MQ=255)
             ggtgctGTGGCGGAAGTTTACGCCAGTAGCGATGCCCTCGAGAAGTTTGTTTAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGTCCTGCTGTAATATTACACCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGa                                                                                                                                        <  1:1281392/149‑1 (MQ=255)
             cgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTTATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTGa                                                                                                                                        <  1:419988/150‑1 (MQ=255)
               tgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAAc                                                                                                                                      <  2:593196/150‑1 (MQ=255)
                gcgGTGGCGTAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCTACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACg                                                                                                                                     <  1:1094536/150‑1 (MQ=255)
                gcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGACCCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACg                                                                                                                                     <  1:114041/150‑1 (MQ=255)
                gcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACg                                                                                                                                     <  1:1500318/150‑1 (MQ=255)
                 cgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATCAACCTCGACCCTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGACGCTGAACGt                                                                                                                                    >  2:1694199/1‑150 (MQ=255)
                  ggTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGtt                                                                                                                                   >  2:923295/1‑150 (MQ=255)
                   gTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTGAACgggg                                                                                                                                  <  1:1262820/150‑4 (MQ=39)
                   gTGGCGGAAGTTTACGCCAGTAGCGATGCCAACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGCCCGTTTCGACCTGCTGTAATCTGACCCCGTACAGCGCCTGCTTGCTGGCAGTCGCTCAACGtaa                                                                                                                                  >  1:1131465/1‑148 (MQ=255)
                    gggCGGAAGTTTACGCCAGTAGCGATGCCCACGATAAGTTTGTTAAAGACTTCGGGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCt                                                                                                                                 <  2:214245/149‑1 (MQ=255)
                      gCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCttt                                                                                                                               <  2:1636605/150‑1 (MQ=255)
                       cGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTa                                                                                                                              >  2:708193/1‑150 (MQ=255)
                        ggAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTAc                                                                                                                             >  2:745743/1‑150 (MQ=255)
                          aaGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCa                                                                                                                           >  1:752666/1‑150 (MQ=255)
                            gTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGc                                                                                                                         >  1:522076/1‑150 (MQ=255)
                                 cGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGtata                                                                                                                    >  2:734503/1‑150 (MQ=255)
                                   ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                                  >  1:1725506/1‑150 (MQ=255)
                                   ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                                  >  1:1587226/1‑150 (MQ=255)
                                     aGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATTAACCTCGACCGTTTCGACGTGCTGTAATCTGACCCCGTTAAGCGGCTGCTTGCTGGCAGTCGCTGAAGGTTCTTTTCAAGCGTATAGTgg                                                                                                                >  2:449714/1‑150 (MQ=255)
                                     aGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTgg                                                                                                                >  2:449712/1‑150 (MQ=255)
                                      gTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTGCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTggg                                                                                                               <  1:1106259/150‑1 (MQ=255)
                                       tAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAATCGCTGAACGTTCTTTACCAGCGTATAGTGGGc                                                                                                              >  1:292023/1‑150 (MQ=255)
                                          cGATGCCCACGAGAAGGTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGGGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaa                                                                                                           <  1:886487/150‑1 (MQ=255)
                                            aTGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaacg                                                                                                         <  2:1805422/150‑1 (MQ=255)
                                             tGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCCGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaacga                                                                                                        >  1:1266347/1‑150 (MQ=255)
                                                 cACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAAc                                                                                                    >  2:1412421/1‑150 (MQ=255)
                                                  aCGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACt                                                                                                   <  1:625457/150‑1 (MQ=255)
                                                     agaAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTaca                                                                                                >  2:1652776/1‑150 (MQ=255)
                                                      gaAGTTTGTTAAAGACTTCGTGGCGGCATGTGTGAAAGTCATGAACCTCGACCGTTTCTACCTGCTGTAATCTGCCCCCGTTCCGCGGCTGCTAGCTGGCAGTCGCTGAACGTTCTTTCACCGCGGATATTGAGCGAACGAAAACTacac                                                                                               >  1:280336/1‑150 (MQ=255)
                                                             gTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCATCGTATAGTGGGCGAACGAAAACTACACACTGGAt                                                                                        >  1:53454/1‑150 (MQ=255)
                                                              ttAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtc                                                                                       <  1:1072069/150‑1 (MQ=255)
                                                               tAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGCCCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtct                                                                                      >  2:304526/1‑150 (MQ=255)
                                                               tAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTTCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtct                                                                                      >  2:1393608/1‑150 (MQ=255)
                                                                aaaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctc                                                                                     >  1:70221/1‑150 (MQ=255)
                                                                 aaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctct                                                                                    <  1:623887/150‑1 (MQ=255)
                                                                   gACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCa                                                                                  <  1:1405517/150‑1 (MQ=255)
                                                                     cTTCGTGGCGGCATGGGGGAAAGTGATGAACCTCGACCGGTTCGCCCTGATGTAAGCTGACCACGTGCATCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTCCCCGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATg                                                                                <  1:794551/150‑1 (MQ=255)
                                                                     attCGGGGCTGCATGGGTGAAAGTGATGAACCTCGACCGGGGCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGGATAGTGGGAGAACGAAAACTACACACTGGATCTCTCATg                                                                                <  1:787766/149‑1 (MQ=255)
                                                                        cGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGttt                                                                             >  2:1330683/1‑148 (MQ=255)
                                                                        cGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCt                                                                             >  2:496364/1‑150 (MQ=255)
                                                                              ggcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCa                                                                       <  2:1421403/150‑1 (MQ=255)
                                                                               gcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGATAACTACACACTGGATCTCTCATGTCTGCCGCAg                                                                      <  1:997139/150‑1 (MQ=255)
                                                                                cATGGGTGAAAGTGATGAACATCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAgg                                                                     >  2:114659/1‑150 (MQ=255)
                                                                                  tGGGTGAAAGTGTTGAACCTCGACCGTTTGGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGATCGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGaa                                                                   <  2:769189/150‑1 (MQ=255)
                                                                                   gggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGaaa                                                                  >  2:393358/1‑150 (MQ=255)
                                                                                    ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAag                                                                 <  1:950240/150‑1 (MQ=255)
                                                                                    ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAag                                                                 <  1:1761128/150‑1 (MQ=255)
                                                                                    ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTATCATGTCTGCCGCAGGAAag                                                                 >  2:338799/1‑150 (MQ=255)
                                                                                     gTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGGCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAaga                                                                <  1:469211/150‑1 (MQ=255)
                                                                                      tGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCCGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAagag                                                               <  1:465746/150‑1 (MQ=255)
                                                                                         aaGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCac                                                            >  1:354031/1‑149 (MQ=255)
                                                                                          aGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAc                                                           <  1:21620/150‑1 (MQ=255)
                                                                                           gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAct                                                          <  1:449712/150‑2 (MQ=255)
                                                                                           gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGGCTGCCGCAGGAAAGAGCAAcc                                                          <  2:898691/150‑1 (MQ=255)
                                                                                               tgaACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACt                                                      <  1:33820/150‑1 (MQ=255)
                                                                                                       gACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGACCGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAATAGCAACCCACTGGCAATCa                                              >  1:1644723/1‑150 (MQ=255)
                                                                                                        aCCGTGTCGACCTGATGTAAGCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGGTGAACGGGCATTACCAGCGGATAGTGGGCGAACGAAAACTAAACACAGGATCTCTAATGTATGACGCAGGAAAAAGCAACCCACGGGCAAGCaa                                             >  2:1369357/1‑149 (MQ=255)
                                                                                                                gACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTgg                                     <  1:123205/150‑1 (MQ=255)
                                                                                                                 aCCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGt                                    >  2:1296713/1‑150 (MQ=255)
                                                                                                                  ccTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGtt                                   >  2:196403/1‑150 (MQ=255)
                                                                                                                  ccTGCTGTAATCTGACCCCGTTCAGCGGCTGATTGCTGGCAGTCGCTGAACGTTATTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATATCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGAAATAAGTGGACTGGtt                                   >  1:25927/1‑150 (MQ=255)
                                                                                                                    tgctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTTTAGTGGGCGAACGAAAACTACACCCTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgg                                 >  1:1319769/1‑149 (MQ=255)
                                                                                                                      ctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGc                               <  1:1607037/150‑1 (MQ=255)
                                                                                                                        gTAATCCGACCCCGTTCAGCGTCGGCGTGCTGGCAGTCGCTGAACGTTCTTTACCCGCGTATAGTGGGCGAACGAACACTCTACACTGGATCTCTCATGTCTGCCGCAGGAAAGCGCAACCCACTGGCAATCAGTGGCCTGGGTGTGCTc                             <  1:1091263/150‑1 (MQ=255)
                                                                                                                             cTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACt                        <  2:27948/150‑1 (MQ=255)
                                                                                                                             cTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACt                        <  2:18373/150‑1 (MQ=255)
                                                                                                                             cTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACt                        <  1:1646121/150‑1 (MQ=255)
                                                                                                                             atGACCCCGTTCAGCGGCTGATTGCTTGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTGATGTCTGCCGCAGGAAAGAGCAACCCAGTGGCAATCAGTGGCCTGGTTGTGCTCACACt                        <  2:1704360/149‑1 (MQ=255)
                                                                                                                               gACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTa                      <  2:454976/150‑1 (MQ=255)
                                                                                                                                agaacGTTCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCAGACTTAt                     >  2:174959/5‑150 (MQ=38)
                                                                                                                                 ccccGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCCATCAGTGGCCTGGTTGTGCTCACACTTATc                    >  2:918959/1‑150 (MQ=255)
                                                                                                                                   ccGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCTTGTCTGCCGCAGGAAAGAGCAACCCTCTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTg                  <  1:188231/150‑1 (MQ=255)
                                                                                                                                      ttCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCGGCGTATAGTGGGCGAACGAAAACTACACCCTGGATCTCTCATGTCGGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCCCTCGTATCTGCAg               >  2:109546/1‑150 (MQ=255)
                                                                                                                                      ttCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAg               >  2:817494/1‑150 (MQ=255)
                                                                                                                                       tCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGt              <  2:661090/150‑1 (MQ=255)
                                                                                                                                         aGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTta            >  1:707694/1‑150 (MQ=255)
                                                                                                                                         aGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTta            >  1:1791824/1‑150 (MQ=255)
                                                                                                                                          gCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGTCCTGGTTGTGCTCACACTTATCTGGAGTtat           <  1:457310/150‑1 (MQ=255)
                                                                                                                                               tgcttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTg      <  1:1393608/150‑1 (MQ=255)
                                                                                                                                               tgcttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTg      >  2:1299531/1‑150 (MQ=255)
                                                                                                                                                gcttgctGGCAGTCGCTGTACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTgg     <  2:1131465/150‑1 (MQ=255)
                                                                                                                                                 cttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGa    <  2:691012/150‑1 (MQ=255)
                                                                                                                                                 cttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGa    >  1:335718/1‑150 (MQ=255)
                                                                                                                                                   tgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGAtt  >  2:1628774/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TAACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATT  >  NC_000913/4135892‑4136188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: