Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,136,043 G→A 6.5% intergenic (+28/‑65) katG → / → yijE catalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,136,0430GA 6.5% 381.2 / 10.3 123intergenic (+28/‑65)katG/yijEcatalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein
Reads supporting (aligned to +/- strand):  ref base G (56/58);  new base A (4/4);  total (60/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

Only 100 of 131 total aligned reads displayed.

ACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCA  >  NC_000913/4135894‑4136192
                                                                                                                                                     |                                                                                                                                                     
aCTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTg                                                                                                                                                       <  1:1625032/150‑1 (MQ=255)
aCTCCGTCCTGCGTGCGGTGCCGGAAGTTTACGCCAGTAGCGATTCCCACGACAAGTTTGTTAAAGACTTCGTCGCGGCATGGGTGAAAGTGATGAACCTCGACAGTTTCGAACTGCTGTAATCTGACACCGTTAAGAGGCTGCTTGCTg                                                                                                                                                       >  1:1704360/1‑150 (MQ=255)
 cTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTgg                                                                                                                                                      >  1:27948/1‑150 (MQ=255)
 cTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTgg                                                                                                                                                      >  1:1381989/1‑150 (MQ=255)
   ccGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGCCCCTCGCCCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCa                                                                                                                                                    >  1:1290535/1‑150 (MQ=255)
       ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGCCCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                                >  2:169126/1‑150 (MQ=255)
       ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                                >  1:454976/1‑150 (MQ=255)
       ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                                >  2:626500/1‑150 (MQ=255)
       ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                                >  2:511228/1‑150 (MQ=255)
        cTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGc                                                                                                                                               >  2:21620/1‑150 (MQ=255)
         tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGTCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGc                                                                                                                                                     >  2:277836/1‑143 (MQ=255)
         tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCt                                                                                                                                              <  1:566077/150‑1 (MQ=255)
         tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCt                                                                                                                                              <  1:1065436/150‑1 (MQ=255)
         tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGCTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAAGCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCt                                                                                                                                              <  1:84909/150‑1 (MQ=255)
           cgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTTATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTGa                                                                                                                                            <  1:419988/150‑1 (MQ=255)
             tgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAAc                                                                                                                                          <  2:593196/150‑1 (MQ=255)
              gcgGTGGCGTAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCTACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACg                                                                                                                                         <  1:1094536/150‑1 (MQ=255)
              gcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGACCCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACg                                                                                                                                         <  1:114041/150‑1 (MQ=255)
              gcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACg                                                                                                                                         <  1:1500318/150‑1 (MQ=255)
               cgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATCAACCTCGACCCTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGACGCTGAACGt                                                                                                                                        >  2:1694199/1‑150 (MQ=255)
                ggTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGtt                                                                                                                                       >  2:923295/1‑150 (MQ=255)
                 gTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTGAACgggg                                                                                                                                      <  1:1262820/150‑4 (MQ=39)
                 gTGGCGGAAGTTTACGCCAGTAGCGATGCCAACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGCCCGTTTCGACCTGCTGTAATCTGACCCCGTACAGCGCCTGCTTGCTGGCAGTCGCTCAACGtaa                                                                                                                                      >  1:1131465/1‑148 (MQ=255)
                  tGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCt                                                                                                                                     >  1:1611804/1‑150 (MQ=255)
                  gggCGGAAGTTTACGCCAGTAGCGATGCCCACGATAAGTTTGTTAAAGACTTCGGGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCt                                                                                                                                     <  2:214245/149‑1 (MQ=255)
                    gCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCttt                                                                                                                                   <  2:1636605/150‑1 (MQ=255)
                     cGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTa                                                                                                                                  >  2:708193/1‑150 (MQ=255)
                      ggAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTAc                                                                                                                                 >  2:745743/1‑150 (MQ=255)
                        aaGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCa                                                                                                                               >  1:752666/1‑150 (MQ=255)
                          gTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGc                                                                                                                             >  1:522076/1‑150 (MQ=255)
                               cGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGtata                                                                                                                        >  2:734503/1‑150 (MQ=255)
                                 ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                                      >  1:1587226/1‑150 (MQ=255)
                                 ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                                      >  1:1725506/1‑150 (MQ=255)
                                 ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                                      >  1:752573/1‑150 (MQ=255)
                                   aGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATTAACCTCGACCGTTTCGACGTGCTGTAATCTGACCCCGTTAAGCGGCTGCTTGCTGGCAGTCGCTGAAGGTTCTTTTCAAGCGTATAGTgg                                                                                                                    >  2:449714/1‑150 (MQ=255)
                                   aGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTgg                                                                                                                    >  2:449712/1‑150 (MQ=255)
                                    gTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTGCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTggg                                                                                                                   <  1:1106259/150‑1 (MQ=255)
                                        cGATGCCCACGAGAAGGTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGGGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaa                                                                                                               <  1:886487/150‑1 (MQ=255)
                                          aTGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaacg                                                                                                             <  2:1805422/150‑1 (MQ=255)
                                           tGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCCGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaacga                                                                                                            >  1:1266347/1‑150 (MQ=255)
                                               cACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAAc                                                                                                        >  2:1412421/1‑150 (MQ=255)
                                                aCGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACt                                                                                                       <  1:625457/150‑1 (MQ=255)
                                                   agaAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTaca                                                                                                    >  2:1652776/1‑150 (MQ=255)
                                                    gaAGTTTGTTAAAGACTTCGTGGCGGCATGTGTGAAAGTCATGAACCTCGACCGTTTCTACCTGCTGTAATCTGCCCCCGTTCCGCGGCTGCTAGCTGGCAGTCGCTGAACGTTCTTTCACCGCGGATATTGAGCGAACGAAAACTacac                                                                                                   >  1:280336/1‑150 (MQ=255)
                                                           gTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCATCGTATAGTGGGCGAACGAAAACTACACACTGGAt                                                                                            >  1:53454/1‑150 (MQ=255)
                                                           gTTAAAGACTTCGTGGCGGCAGGCGTGAAAGTGCTGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAt                                                                                            <  1:290283/150‑1 (MQ=255)
                                                            ttAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtc                                                                                           <  1:1072069/150‑1 (MQ=255)
                                                             tAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGCCCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtct                                                                                          >  2:304526/1‑150 (MQ=255)
                                                              aaaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctc                                                                                         >  1:70221/1‑150 (MQ=255)
                                                              aaaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGGTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctc                                                                                         <  1:80568/150‑1 (MQ=255)
                                                               aaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGCCCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctct                                                                                        >  1:1669836/1‑150 (MQ=255)
                                                                 gACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCa                                                                                      <  1:1405517/150‑1 (MQ=255)
                                                                   cTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATg                                                                                    <  2:1611804/150‑1 (MQ=255)
                                                                   attCGGGGCTGCATGGGTGAAAGTGATGAACCTCGACCGGGGCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGGATAGTGGGAGAACGAAAACTACACACTGGATCTCTCATg                                                                                    <  1:787766/149‑1 (MQ=255)
                                                                     tCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTc                                                                                  >  1:1398220/1‑150 (MQ=255)
                                                                      cGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGttt                                                                                 >  2:1330683/1‑148 (MQ=255)
                                                                            ggcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCa                                                                           <  2:1421403/150‑1 (MQ=255)
                                                                             gcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGATAACTACACACTGGATCTCTCATGTCTGCCGCAg                                                                          <  1:997139/150‑1 (MQ=255)
                                                                              cATGTGTGAAAGTGATGAACCTCGACCGTTTCTACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACTAAAACTACACACTGTATCTCTCATGTCTGCCGCAgg                                                                         <  1:254959/150‑1 (MQ=255)
                                                                                tGGGTGAAAGTGTTGAACCTCGACCGTTTGGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGATCGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGaa                                                                       <  2:769189/150‑1 (MQ=255)
                                                                                 gggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGaaa                                                                      >  2:393358/1‑150 (MQ=255)
                                                                                  ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAag                                                                     <  1:1761128/150‑1 (MQ=255)
                                                                                  ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTATCATGTCTGCCGCAGGAAag                                                                     >  2:338799/1‑150 (MQ=255)
                                                                                    tGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCCGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAagag                                                                   <  1:465746/150‑1 (MQ=255)
                                                                                       aaGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCac                                                                >  1:354031/1‑149 (MQ=255)
                                                                                        aGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAc                                                               <  1:21620/150‑1 (MQ=255)
                                                                                        aGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACAATAGTATAGTGGTCGAACGATATCTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAc                                                               <  2:1381989/150‑1 (MQ=255)
                                                                                         gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAct                                                              <  1:449712/150‑2 (MQ=255)
                                                                                         gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAcc                                                              >  2:1283702/1‑150 (MQ=255)
                                                                                         gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGGCTGCCGCAGGAAAGAGCAAcc                                                              <  2:898691/150‑1 (MQ=255)
                                                                                             tgaACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACt                                                          <  1:33820/150‑1 (MQ=255)
                                                                                                  cTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCaa                                                     <  2:1059925/150‑1 (MQ=255)
                                                                                                     gACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGACCGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAATAGCAACCCACTGGCAATCa                                                  >  1:1644723/1‑150 (MQ=255)
                                                                                                      aCCGTGTCGACCTGATGTAAGCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGGTGAACGGGCATTACCAGCGGATAGTGGGCGAACGAAAACTAAACACAGGATCTCTAATGTATGACGCAGGAAAAAGCAACCCACGGGCAAGCaa                                                 >  2:1369357/1‑149 (MQ=255)
                                                                                                              gACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTgg                                         <  1:123205/150‑1 (MQ=255)
                                                                                                               aCCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGt                                        >  2:1296713/1‑150 (MQ=255)
                                                                                                               aCCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAATCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGt                                        <  2:292023/150‑1 (MQ=255)
                                                                                                                ccTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGtt                                       >  2:196403/1‑150 (MQ=255)
                                                                                                                ccTGCTGTAATCTGACCCCGTTCAGCGGCTGATTGCTGGCAGTCGCTGAACGTTATTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATATCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGAAATAAGTGGACTGGtt                                       >  1:25927/1‑150 (MQ=255)
                                                                                                                  tgctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTTTAGTGGGCGAACGAAAACTACACCCTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgg                                     >  1:1319769/1‑149 (MQ=255)
                                                                                                                   gctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgtg                                    <  1:1796710/150‑1 (MQ=255)
                                                                                                                      gTAATCCGACCCCGTTCAGCGTCGGCGTGCTGGCAGTCGCTGAACGTTCTTTACCCGCGTATAGTGGGCGAACGAACACTCTACACTGGATCTCTCATGTCTGCCGCAGGAAAGCGCAACCCACTGGCAATCAGTGGCCTGGGTGTGCTc                                 <  1:1091263/150‑1 (MQ=255)
                                                                                                                           cTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACt                            <  2:18373/150‑1 (MQ=255)
                                                                                                                           atGACCCCGTTCAGCGGCTGATTGCTTGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTGATGTCTGCCGCAGGAAAGAGCAACCCAGTGGCAATCAGTGGCCTGGTTGTGCTCACACt                            <  2:1704360/149‑1 (MQ=255)
                                                                                                                             gACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTa                          <  2:454976/150‑1 (MQ=255)
                                                                                                                              agaacGTTCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCAGACTTAt                         >  2:174959/5‑150 (MQ=38)
                                                                                                                               ccccGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCCATCAGTGGCCTGGTTGTGCTCACACTTATc                        >  2:918959/1‑150 (MQ=255)
                                                                                                                                 ccGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCTTGTCTGCCGCAGGAAAGAGCAACCCTCTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTg                      <  1:188231/150‑1 (MQ=255)
                                                                                                                                    ttCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAg                   <  1:600496/150‑1 (MQ=255)
                                                                                                                                    ttCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCGGCGTATAGTGGGCGAACGAAAACTACACCCTGGATCTCTCATGTCGGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCCCTCGTATCTGCAg                   >  2:109546/1‑150 (MQ=255)
                                                                                                                                    ttCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAg                   >  2:817494/1‑150 (MQ=255)
                                                                                                                                       aGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTta                >  1:707694/1‑150 (MQ=255)
                                                                                                                                       aGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTta                >  1:1791824/1‑150 (MQ=255)
                                                                                                                                        gCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGTCCTGGTTGTGCTCACACTTATCTGGAGTtat               <  1:457310/150‑1 (MQ=255)
                                                                                                                                              gcttgctGGCAGTCGCTGTACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTgg         <  2:1131465/150‑1 (MQ=255)
                                                                                                                                               cttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGa        >  1:335718/1‑150 (MQ=255)
                                                                                                                                               cttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGa        <  2:691012/150‑1 (MQ=255)
                                                                                                                                                    tGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTc   <  2:1290535/150‑1 (MQ=255)
                                                                                                                                                     ggCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGTCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCa  <  1:304526/150‑1 (MQ=255)
                                                                                                                                                     ggCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCa  <  2:1671961/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTCCGTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCA  >  NC_000913/4135894‑4136192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: