Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,136,048 T→C 6.6% intergenic (+33/‑60) katG → / → yijE catalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,136,0480TC 6.6% 350.3 / 11.6 123intergenic (+33/‑60)katG/yijEcatalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein
Reads supporting (aligned to +/- strand):  ref base T (53/60);  new base C (4/4);  total (58/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 123 total aligned reads displayed.

CCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATG  >  NC_000913/4135901‑4136194
                                                                                                                                                   |                                                                                                                                                  
ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGCCCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                                  >  2:169126/1‑150 (MQ=255)
ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                                  >  1:454976/1‑150 (MQ=255)
ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                                  >  2:511228/1‑150 (MQ=255)
ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                                  >  2:626500/1‑150 (MQ=255)
 cTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGc                                                                                                                                                 >  2:21620/1‑150 (MQ=255)
  tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCt                                                                                                                                                <  1:566077/150‑1 (MQ=255)
  tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCt                                                                                                                                                <  1:1065436/150‑1 (MQ=255)
  tgcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGCTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAAGCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCt                                                                                                                                                <  1:84909/150‑1 (MQ=255)
    ggtgctGTGGCGGAAGTTTACGCCAGTAGCGATGCCCTCGAGAAGTTTGTTTAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGTCCTGCTGTAATATTACACCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGa                                                                                                                                              <  1:1281392/149‑1 (MQ=255)
    cgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTTATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTGa                                                                                                                                              <  1:419988/150‑1 (MQ=255)
      tgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAAc                                                                                                                                            <  2:593196/150‑1 (MQ=255)
       gcgGTGGCGTAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCTACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACg                                                                                                                                           <  1:1094536/150‑1 (MQ=255)
       gcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGACCCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACg                                                                                                                                           <  1:114041/150‑1 (MQ=255)
        cgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATCAACCTCGACCCTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGACGCTGAACGt                                                                                                                                          >  2:1694199/1‑150 (MQ=255)
          gTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTGAACgggg                                                                                                                                        <  1:1262820/150‑4 (MQ=39)
           tGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCt                                                                                                                                       >  1:1611804/1‑150 (MQ=255)
             gCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCttt                                                                                                                                     <  2:1636605/150‑1 (MQ=255)
              cGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTa                                                                                                                                    >  2:708193/1‑150 (MQ=255)
               ggAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTAc                                                                                                                                   >  2:745743/1‑150 (MQ=255)
                 aaGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCa                                                                                                                                 >  1:752666/1‑150 (MQ=255)
                        cGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGtata                                                                                                                          >  2:734503/1‑150 (MQ=255)
                          ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                                        >  1:1725506/1‑150 (MQ=255)
                          ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                                        >  1:1587226/1‑150 (MQ=255)
                            aGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATTAACCTCGACCGTTTCGACGTGCTGTAATCTGACCCCGTTAAGCGGCTGCTTGCTGGCAGTCGCTGAAGGTTCTTTTCAAGCGTATAGTgg                                                                                                                      >  2:449714/1‑150 (MQ=255)
                            aGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTgg                                                                                                                      >  2:449712/1‑150 (MQ=255)
                             gTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTGCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTggg                                                                                                                     <  1:1106259/150‑1 (MQ=255)
                              tAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAATCGCTGAACGTTCTTTACCAGCGTATAGTGGGc                                                                                                                    >  1:292023/1‑150 (MQ=255)
                                   aTGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaacg                                                                                                               <  2:1805422/150‑1 (MQ=255)
                                   aTGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaacg                                                                                                               <  2:175379/150‑1 (MQ=255)
                                        cACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAAc                                                                                                          >  2:1412421/1‑150 (MQ=255)
                                            agaAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTaca                                                                                                      >  2:1652776/1‑150 (MQ=255)
                                                    gTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCATCGTATAGTGGGCGAACGAAAACTACACACTGGAt                                                                                              >  1:53454/1‑150 (MQ=255)
                                                    gTTAAAGACTTCGTGGCGGCAGGCGTGAAAGTGCTGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAt                                                                                              <  1:290283/150‑1 (MQ=255)
                                                     ttAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtc                                                                                             <  1:1072069/150‑1 (MQ=255)
                                                      tAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGCCCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtct                                                                                            >  2:304526/1‑150 (MQ=255)
                                                      tAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTTCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtct                                                                                            >  2:1393608/1‑150 (MQ=255)
                                                       aaaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctc                                                                                           >  1:70221/1‑150 (MQ=255)
                                                       aaaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGGTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctc                                                                                           <  1:80568/150‑1 (MQ=255)
                                                        aaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGCCCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctct                                                                                          >  1:1669836/1‑150 (MQ=255)
                                                        aaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctct                                                                                          <  1:623887/150‑1 (MQ=255)
                                                          gACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCa                                                                                        >  2:1689717/1‑150 (MQ=255)
                                                          gACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCa                                                                                        <  1:1405517/150‑1 (MQ=255)
                                                            cTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATg                                                                                      <  2:1611804/150‑1 (MQ=255)
                                                            cTTCGTGGCGGCATGGGGGAAAGTGATGAACCTCGACCGGTTCGCCCTGATGTAAGCTGACCACGTGCATCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTCCCCGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATg                                                                                      <  1:794551/150‑1 (MQ=255)
                                                            attCGGGGCTGCATGGGTGAAAGTGATGAACCTCGACCGGGGCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGGATAGTGGGAGAACGAAAACTACACACTGGATCTCTCATg                                                                                      <  1:787766/149‑1 (MQ=255)
                                                              tCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTc                                                                                    >  1:1398220/1‑150 (MQ=255)
                                                               cGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGttt                                                                                   >  2:1330683/1‑148 (MQ=255)
                                                               cGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCt                                                                                   >  2:496364/1‑150 (MQ=255)
                                                                  ggcggcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGcc                                                                                >  2:846695/1‑150 (MQ=255)
                                                                      gcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGATAACTACACACTGGATCTCTCATGTCTGCCGCAg                                                                            <  1:997139/150‑1 (MQ=255)
                                                                       cATGTGTGAAAGTGATGAACCTCGACCGTTTCTACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACTAAAACTACACACTGTATCTCTCATGTCTGCCGCAgg                                                                           <  1:254959/150‑1 (MQ=255)
                                                                       cATGGGTGAAAGTGATGAACATCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAgg                                                                           >  2:114659/1‑150 (MQ=255)
                                                                          gggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGaaa                                                                        >  2:393358/1‑150 (MQ=255)
                                                                           ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAag                                                                       <  1:1761128/150‑1 (MQ=255)
                                                                           ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAag                                                                       <  1:950240/150‑1 (MQ=255)
                                                                            gTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGGCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAaga                                                                      <  1:469211/150‑1 (MQ=255)
                                                                             tGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCCGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAagag                                                                     <  1:465746/150‑1 (MQ=255)
                                                                               aaaGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTCCCAGCGTATAGTGGTCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCa                                                                   <  1:351710/150‑1 (MQ=255)
                                                                                aaGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCac                                                                  >  1:354031/1‑149 (MQ=255)
                                                                                 aGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAc                                                                 <  1:21620/150‑1 (MQ=255)
                                                                                 aGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACAATAGTATAGTGGTCGAACGATATCTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAc                                                                 <  2:1381989/150‑1 (MQ=255)
                                                                                  ttgatgaACCTCGACCTTTTCTACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGTCAGTCGCATAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGTATCTCTCATGTCTGCCTCAGGAAAGAGCAAcc                                                                <  1:449714/149‑1 (MQ=255)
                                                                                  gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAcc                                                                >  2:1283702/1‑150 (MQ=255)
                                                                                  gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGGCTGCCGCAGGAAAGAGCAAcc                                                                <  2:898691/150‑1 (MQ=255)
                                                                                           cTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCaa                                                       <  2:1059925/150‑1 (MQ=255)
                                                                                              gACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGACCGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAATAGCAACCCACTGGCAATCa                                                    >  1:1644723/1‑150 (MQ=255)
                                                                                               aCCGTGTCGACCTGATGTAAGCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGGTGAACGGGCATTACCAGCGGATAGTGGGCGAACGAAAACTAAACACAGGATCTCTAATGTATGACGCAGGAAAAAGCAACCCACGGGCAAGCaa                                                   >  2:1369357/1‑149 (MQ=255)
                                                                                                       gACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTgg                                           <  2:197017/150‑1 (MQ=255)
                                                                                                       gACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTgg                                           <  1:123205/150‑1 (MQ=255)
                                                                                                        aCCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGt                                          >  2:1296713/1‑150 (MQ=255)
                                                                                                        aCCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAATCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGt                                          <  2:292023/150‑1 (MQ=255)
                                                                                                         ccTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGtt                                         >  2:196403/1‑150 (MQ=255)
                                                                                                         ccTGCTGTAATCTGACCCCGTTCAGCGGCTGATTGCTGGCAGTCGCTGAACGTTATTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATATCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGAAATAAGTGGACTGGtt                                         >  1:25927/1‑150 (MQ=255)
                                                                                                           tgctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTTTAGTGGGCGAACGAAAACTACACCCTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgg                                       >  1:1319769/1‑149 (MQ=255)
                                                                                                            gctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgtg                                      <  1:1796710/150‑1 (MQ=255)
                                                                                                             ctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGc                                     >  2:357780/1‑150 (MQ=255)
                                                                                                             ctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGc                                     <  1:1607037/150‑1 (MQ=255)
                                                                                                               gTAATCCGACCCCGTTCAGCGTCGGCGTGCTGGCAGTCGCTGAACGTTCTTTACCCGCGTATAGTGGGCGAACGAACACTCTACACTGGATCTCTCATGTCTGCCGCAGGAAAGCGCAACCCACTGGCAATCAGTGGCCTGGGTGTGCTc                                   <  1:1091263/150‑1 (MQ=255)
                                                                                                                    cTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACt                              <  2:18373/150‑1 (MQ=255)
                                                                                                                    cTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACt                              <  2:27948/150‑1 (MQ=255)
                                                                                                                    atGACCCCGTTCAGCGGCTGATTGCTTGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTGATGTCTGCCGCAGGAAAGAGCAACCCAGTGGCAATCAGTGGCCTGGTTGTGCTCACACt                              <  2:1704360/149‑1 (MQ=255)
                                                                                                                        ccccGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCCATCAGTGGCCTGGTTGTGCTCACACTTATc                          >  2:918959/1‑150 (MQ=255)
                                                                                                                          ccGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCTTGTCTGCCGCAGGAAAGAGCAACCCTCTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTg                        <  1:188231/150‑1 (MQ=255)
                                                                                                                             ttCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAg                     <  1:600496/150‑1 (MQ=255)
                                                                                                                             ttCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCGGCGTATAGTGGGCGAACGAAAACTACACCCTGGATCTCTCATGTCGGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCCCTCGTATCTGCAg                     >  2:109546/1‑150 (MQ=255)
                                                                                                                             ttCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAg                     >  2:817494/1‑150 (MQ=255)
                                                                                                                             ttCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATAAGTGGCCTGGTTGTGCTCACACTTATCTGGAg                     >  2:738957/1‑150 (MQ=255)
                                                                                                                              tCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGt                    <  2:661090/150‑1 (MQ=255)
                                                                                                                                aGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTta                  >  1:707694/1‑150 (MQ=255)
                                                                                                                                 gCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGTCCTGGTTGTGCTCACACTTATCTGGAGTtat                 <  1:457310/150‑1 (MQ=255)
                                                                                                                                    gctgctTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGc              <  1:1161508/150‑1 (MQ=255)
                                                                                                                                      tgcttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTg            <  1:1393608/150‑1 (MQ=255)
                                                                                                                                        cttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGa          >  1:335718/1‑150 (MQ=255)
                                                                                                                                          tgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGAtt        >  2:1628774/1‑150 (MQ=255)
                                                                                                                                            ctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGAtttt      >  1:1751446/1‑150 (MQ=255)
                                                                                                                                             tGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTc     <  2:1290535/150‑1 (MQ=255)
                                                                                                                                              ggCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGTCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCa    <  1:304526/150‑1 (MQ=255)
                                                                                                                                              ggCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCa    >  1:735449/1‑150 (MQ=255)
                                                                                                                                               gCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCAt   <  1:745743/150‑1 (MQ=255)
                                                                                                                                                cAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATg  <  1:352782/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATG  >  NC_000913/4135901‑4136194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: