Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 454,856 G→A 5.8% intergenic (+67/‑277) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913454,8560GA 5.8% 323.7 / 5.6 107intergenic (+67/‑277)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (44/55);  new base A (4/2);  total (49/58)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.45e-01

Only 100 of 108 total aligned reads displayed.

CTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAA  >  NC_000913/454707‑455004
                                                                                                                                                     |                                                                                                                                                    
cTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGATGCTAAAGATTTTATGAAAAACGTCCTGCg                                                                                                                                                      >  1:402100/1‑150 (MQ=255)
      cTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgt                                                                                                                                                >  1:228314/1‑150 (MQ=255)
      cTATTAAGGAGTGGGAAGGGTTGCAGGACACCCTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCAGTATGTTGCTAAATATTTTATGAAAAACGGCATGCGGGCCgt                                                                                                                                                >  2:1531382/1‑150 (MQ=255)
       tATTACGGAGTGGGACGGGTGGGAGGACACCGTCTTTGCCTCTCCTCCCTGGCGGGCAGCAGGCAGCATCGCGAAACAACGCATTTGCAACTGTCGGCGCTTTTCCTGTATGTTGCTACAGATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                                               <  2:140884/150‑1 (MQ=255)
       tATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGCAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                                               <  2:1104732/150‑1 (MQ=255)
        aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgttt                                                                                                                                              <  1:1052297/150‑1 (MQ=255)
        aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGACGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttt                                                                                                                                              >  1:1651672/1‑150 (MQ=255)
         ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCGCGCAGGCCgtttt                                                                                                                                             <  1:1801796/150‑1 (MQ=255)
          tAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttg                                                                                                                                            >  2:1177703/1‑150 (MQ=255)
           aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgt                                                                                                                                           >  2:1506896/1‑150 (MQ=255)
           aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgt                                                                                                                                           <  1:1017855/150‑1 (MQ=255)
           aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgt                                                                                                                                           <  1:1466947/150‑1 (MQ=255)
            aGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgtt                                                                                                                                          <  2:94543/150‑1 (MQ=255)
            aGGAGTGGGAAGGGGTGCAGGAGACCGTCTTTGCCTCTCCTCCCTTTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAATGATTTTATGAAAAACGGCCTGCGGGCCgttttgtt                                                                                                                                          <  1:651593/150‑1 (MQ=255)
             ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgttt                                                                                                                                         >  2:836153/1‑150 (MQ=255)
              gAGTGGGCAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTGTTCCATTATGTTGATAAAGATTTTATGACAAACGGCCCGCCGGCCgtttttcata                                                                                                                                        <  1:1135333/150‑6 (MQ=255)
              gAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgtttt                                                                                                                                        >  1:1482208/1‑150 (MQ=255)
                 tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTc                                                                                                                                     <  1:377711/150‑1 (MQ=255)
                 tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTc                                                                                                                                     <  1:1502375/150‑1 (MQ=255)
                 ggggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGGGCAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGGCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTc                                                                                                                                     <  1:1121417/149‑1 (MQ=255)
                  gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGGTTTGTCt                                                                                                                                    >  1:430338/1‑150 (MQ=255)
                  gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTCCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                    >  2:1167937/1‑150 (MQ=255)
                      aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAt                                                                                                                                <  1:517961/150‑1 (MQ=255)
                       gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtt                                                                                                                               >  2:565523/1‑150 (MQ=255)
                       gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtt                                                                                                                               >  1:322164/1‑150 (MQ=255)
                        ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAttt                                                                                                                              <  2:1368004/150‑1 (MQ=255)
                          ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTg                                                                                                                            <  1:1423731/150‑1 (MQ=255)
                          ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGAGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAATACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTg                                                                                                                            <  1:1482707/150‑1 (MQ=255)
                           tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgc                                                                                                                           <  2:204922/150‑1 (MQ=255)
                            gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgcg                                                                                                                          >  1:899950/1‑150 (MQ=255)
                            gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgcg                                                                                                                          >  2:431321/1‑150 (MQ=255)
                               ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtt                                                                                                                       <  2:114520/150‑1 (MQ=255)
                                   acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTg                                                                                                                   <  1:1077487/150‑1 (MQ=255)
                                   acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTg                                                                                                                   <  1:510096/150‑1 (MQ=255)
                                   acCGTCTGTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAAAGCATTTTCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTg                                                                                                                   <  2:1236219/150‑1 (MQ=255)
                                    ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGc                                                                                                                  >  2:1740267/1‑150 (MQ=255)
                                    ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGc                                                                                                                  <  2:1778479/150‑1 (MQ=255)
                                     cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGcc                                                                                                                 <  1:1640760/150‑1 (MQ=255)
                                      gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTGTCTGGATTTTGCGCTTTTTGccc                                                                                                                >  1:1544698/1‑150 (MQ=255)
                                      gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGccc                                                                                                                >  2:1110691/1‑150 (MQ=255)
                                        cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAATGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAg                                                                                                              <  2:1599106/150‑1 (MQ=255)
                                        cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAg                                                                                                              <  1:928355/150‑1 (MQ=255)
                                           tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAACCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCGGGATTTTGCGCTTTTTGCCCAGCAt                                                                                                           >  2:1582063/1‑150 (MQ=255)
                                           tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt                                                                                                           >  2:1201107/1‑150 (MQ=255)
                                            gCCTCTCCTCCCTGTCTTTGCGCAGGAAGCTTCGCGTAAAAACGCATTTGCAACTGGCGTCGCTTTTCCAGTATGTTGCTAAATATTTTATGAAAACCGGCCTGAGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCATCAtt                                                                                                          <  1:511736/150‑1 (MQ=255)
                                             ccTCTCCTCCCTGTCTTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                                         <  2:1524921/150‑1 (MQ=255)
                                              tcctgctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                        >  1:1237256/6‑150 (MQ=255)
                                               tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                                                       >  1:217629/1‑150 (MQ=255)
                                                  ccgcccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTGCCAGTATGTTGCTAAAGATTATATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg                                                                                                    <  1:335152/147‑1 (MQ=255)
                                                    tccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaa                                                                                                  <  1:845994/150‑1 (MQ=255)
                                                     cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaa                                                                                                 >  1:1510776/1‑150 (MQ=255)
                                                      ccTGTCGTGGAGCAGCAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCGGCTGGCCGTTTTGTTTTGGCAGGATTTTGCGCTTTTTTCCCAGCATTCAGACGaaaa                                                                                                >  1:1638514/1‑150 (MQ=255)
                                                       cTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAt                                                                                               <  1:1177703/150‑1 (MQ=255)
                                                        tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAtt                                                                                              >  1:673591/1‑150 (MQ=255)
                                                        tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAtt                                                                                              >  2:522973/1‑150 (MQ=255)
                                                           cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGcc                                                                                           <  2:510074/150‑1 (MQ=255)
                                                                 gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGaa                                                                                     >  2:1456027/1‑150 (MQ=255)
                                                                 gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGaa                                                                                     >  1:1692263/1‑150 (MQ=255)
                                                                    ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtg                                                                                  >  2:669209/1‑150 (MQ=255)
                                                                    ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtg                                                                                  >  2:803019/1‑150 (MQ=255)
                                                                    ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACCGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAttt                                                                                  >  2:1414097/1‑149 (MQ=255)
                                                                      aaGCATATCTTAAAAACGCATTTGCAACTGTCGGCGCATTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCTTTCAGACGAAAATTGCCCGGGAATtgtg                                                                                <  1:367624/150‑1 (MQ=255)
                                                                            cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaaaa                                                                          <  1:1721676/150‑1 (MQ=255)
                                                                            cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaaaa                                                                          <  1:1200314/150‑1 (MQ=255)
                                                                              cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGACTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATa                                                                        <  1:954971/150‑1 (MQ=255)
                                                                                 aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGTAATTGTGAAAAAATAcgc                                                                     <  1:1366093/150‑1 (MQ=255)
                                                                                      cGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAg                                                                >  2:1700647/1‑150 (MQ=255)
                                                                                        cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcg                                                              <  1:1629831/150‑1 (MQ=255)
                                                                                           ttGCAACTTTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTAATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAGGAAAATTGCCCGGTAATTGTGAAAAAATACGCGACAgcgcgc                                                           <  1:883652/150‑1 (MQ=255)
                                                                                           ttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgcgc                                                           <  2:252320/150‑1 (MQ=255)
                                                                                           ttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgcgc                                                           <  1:1036406/150‑1 (MQ=255)
                                                                                            tGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCa                                                          <  2:899950/150‑1 (MQ=255)
                                                                                             gCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCaa                                                         >  2:1231279/1‑150 (MQ=255)
                                                                                               aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                                                       <  1:418675/150‑1 (MQ=255)
                                                                                                 cTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc                                                     >  1:1300308/1‑150 (MQ=255)
                                                                                                  tttcgtcgCTTTTCCAGTATGTTTCTAATTATTTTATTAAAAACGTCATGCGGGACGTTTTGTTTTGTCTGGATTTTGCTCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAAcc                                                    <  1:1650898/148‑1 (MQ=255)
                                                                                                       gcgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCTCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                                               <  2:64960/150‑1 (MQ=255)
                                                                                                        cgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTTTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                                              >  1:1721985/1‑150 (MQ=255)
                                                                                                        cgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                                              <  1:1094865/150‑1 (MQ=255)
                                                                                                         gcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                             <  2:1200937/150‑1 (MQ=255)
                                                                                                           ttttCCAGTATGTTGCTAAAGATTTTATGAAAACCTGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                                           <  1:105891/150‑1 (MQ=255)
                                                                                                            tttCCAGTATGTTGCTAAATATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACt                                          <  1:1767951/150‑1 (MQ=255)
                                                                                                            tttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACt                                          <  1:1167937/150‑1 (MQ=255)
                                                                                                             tttcAGTTTGTTGTTAAAGATTTTTTGAAAAACGGCCTGCGGTCCGTTTTGTTTTGTCTTGTTTTTGCGCTTTTTGCCCAGCATTCAGAGGAAAATTGCCCGGGATTTGTGAAAAAATTCGCGACAGCGCGCAATAACCGTTGTTGACTc                                         <  1:537033/147‑1 (MQ=255)
                                                                                                               ccAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGGAATAACCGTTCTCGACTCAt                                       >  2:115892/1‑150 (MQ=255)
                                                                                                               ccAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACAGTTCTCGACACAt                                       >  2:123019/1‑150 (MQ=255)
                                                                                                               ccAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGATTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGATCTCGACTCAt                                       >  1:1036899/1‑150 (MQ=255)
                                                                                                                       ttGCTAAATATTTTATGAAAAAGGCCCTGCGGGCCGTTTTGTTTTGTCTGTTTTTTGCGATTTTTGCCCATCATTCAGACTAAAATTGCCCGGTAATTTTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa                               <  1:1506896/150‑1 (MQ=255)
                                                                                                                         gCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATg                             >  1:979239/1‑150 (MQ=255)
                                                                                                                                   ttATGAAAAACGGCCTTCGGGCGGTTTTGTTTTGTGTGTATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGTTATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAAt                   <  2:1515537/150‑1 (MQ=255)
                                                                                                                                   caaaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAAt                   >  2:52644/7‑150 (MQ=255)
                                                                                                                                       taaaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg               <  1:1821940/149‑1 (MQ=255)
                                                                                                                                       acaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg               >  2:1779906/3‑150 (MQ=255)
                                                                                                                                       acaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTGCGCTTTTTGCCCAGCATTCCGGCGAAAATTGCCCGGGAATTGGGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGGTGCCGGTATTATGCcgg              >  1:1739496/3‑149 (MQ=255)
                                                                                                                                        aaaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTCCCCAGCATTCAGACTAAAATTGCCCGGTAATTGTCAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgc              <  1:845660/150‑1 (MQ=255)
                                                                                                                                         aaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg             >  1:1616795/1‑150 (MQ=255)
                                                                                                                                              ggCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTa        <  2:1482208/150‑1 (MQ=255)
                                                                                                                                               gCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAt       >  1:693181/1‑150 (MQ=255)
                                                                                                                                               gCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTACCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAt       >  2:196410/1‑150 (MQ=255)
                                                                                                                                                    cGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCAATTTGaa  >  1:1685158/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAA  >  NC_000913/454707‑455004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: