Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,241,067 C→T 6.3% S396S (TCG→TCA)  xylE ← D‑xylose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,241,0670CT 6.3% 292.7 / 6.2 96S396S (TCG→TCA) xylED‑xylose transporter
Reads supporting (aligned to +/- strand):  ref base C (35/54);  new base T (3/3);  total (39/57)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGAT  >  NC_000913/4240919‑4241216
                                                                                                                                                    |                                                                                                                                                     
aGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCg                                                                                                                                                      <  1:584063/150‑1 (MQ=255)
aGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCg                                                                                                                                                      <  1:1074900/150‑1 (MQ=255)
aGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCg                                                                                                                                                      <  2:1760209/150‑1 (MQ=255)
aGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCg                                                                                                                                                      <  2:1762168/150‑1 (MQ=255)
 gTAGGAGAAACCGTTCTGGAAATGGGCCACCAGCTAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCATTACCACGAATAGCATTACGGAAGAATTccta                                                                                                                                                     >  1:275519/1‑148 (MQ=255)
  tAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAc                                                                                                                                                    >  2:1252492/1‑150 (MQ=255)
   aGGAGAAACCGTGGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAca                                                                                                                                                   <  1:1066163/150‑1 (MQ=255)
    ggAGAAACCGTTGTGGAAATGGGCCCCCAGCCTGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcag                                                                                                                                                  >  2:939552/1‑150 (MQ=255)
    ggAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcag                                                                                                                                                  >  1:439174/1‑150 (MQ=255)
     gagaAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcagc                                                                                                                                                 <  1:1243876/150‑1 (MQ=255)
          aCCGTTGTGGAAATGGGCCACCAGCCAGGAGGTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAAAAGCATTCGGGAAGATTTCCGACAGCAGTAc                                                                                                                                            <  2:48292/150‑1 (MQ=255)
               tttgGAAATGGGCCACCATCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCTCCACCTCGATAGCCAGCGCTTTACCTCGAATAGCATTCGGGAAGATATCCGACAGCAGTACCCAGc                                                                                                                                       <  1:1172823/148‑1 (MQ=255)
               tgtgGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCCGCGCTTTACCACGAAGAGCATTCGGGAAGATTTCCGACAGCAGTCCCCAGc                                                                                                                                       >  2:82304/1‑150 (MQ=255)
                 tgGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCAGACAGCAGTACCAAGCAt                                                                                                                                     >  2:893467/1‑150 (MQ=255)
                   gAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCCCCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATAc                                                                                                                                   >  1:788178/1‑150 (MQ=255)
                   gAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATAc                                                                                                                                   <  2:422986/150‑1 (MQ=255)
                   gAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATAc                                                                                                                                   >  2:1687074/1‑150 (MQ=255)
                     aaTGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGACACTGGGCCGCCACCGCGATTGCCAGCGCTGTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCg                                                                                                                                 <  2:1400421/150‑1 (MQ=255)
                         ggCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGCCAGCAGTCCCCAGCATACCgtccc                                                                                                                             >  1:93026/1‑146 (MQ=255)
                         ggCCACCAGCCAGGAGTTTTTGTCAATCATCGGGAAGGTCCAGGAGACCAAGTAGTTCGCCCGCCACTGGGCCGCCACCGCGATTGCCAGAGCTTTACAACGAATAGCATTCGGGGAGCTTTCCGAAAGCAGTAAACCGCATCCAGGAcc                                                                                                                             >  1:1649158/1‑150 (MQ=255)
                          gCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACCGCAGTACCCAGCATACCGGAccc                                                                                                                            >  1:1356191/1‑150 (MQ=255)
                           ccaccaGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCTGCCAGCAGTACCCAGCATACCGGAcccc                                                                                                                           >  2:218721/1‑150 (MQ=255)
                           ccaccaGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCTGACAGCAGTACCCAGCATACCGGAcccc                                                                                                                           >  2:212970/1‑150 (MQ=255)
                           ccaccaGCCAGGAGTTTTTGTCCATAATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCTGACAGCAGTACCCAGCATACCGGAcccc                                                                                                                           >  2:1505617/1‑150 (MQ=255)
                             accaGCCAGGAGTTGATGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGATTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAg                                                                                                                         <  1:278904/150‑1 (MQ=255)
                              caagccagGAGTTTTTGTACATCATCTGGAAGGTCCAGGAGACGAAGTAGTTCGCCATCCACTGGGCCGCCACCGCGATTGACAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAgg                                                                                                                        <  1:760255/148‑1 (MQ=255)
                                 gccagGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACa                                                                                                                     >  2:179389/1‑150 (MQ=255)
                                   cagGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATg                                                                                                                   <  2:1589875/150‑1 (MQ=255)
                                   cagGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGAACCCAGGACATg                                                                                                                   >  1:1336688/1‑150 (MQ=255)
                                    agGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATgg                                                                                                                  <  2:439174/150‑1 (MQ=255)
                                     ggAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGc                                                                                                                 <  1:232191/150‑1 (MQ=255)
                                      ttgTTTTTGTCCATCTTCTGGTAGGTCCAGGAGACGTTGTAGTTGGGCTGGCAGTGGGCCGCCACCGCGATTGCCAGCGCTTTACTACGAATAGCTTTCGGGAAGATTTGCGAAAGGAGTTCCCAGCATACCTGACCCCAGGTCATGGct                                                                                                                <  2:628442/148‑2 (MQ=255)
                                            ttGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcg                                                                                                          <  2:22567/150‑1 (MQ=255)
                                            ttGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcg                                                                                                          <  1:1747876/150‑1 (MQ=255)
                                             tGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcgg                                                                                                         >  2:1258685/1‑150 (MQ=255)
                                               tCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCTCCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCa                                                                                                       <  2:1430027/150‑1 (MQ=255)
                                               tCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCa                                                                                                       <  1:203240/150‑1 (MQ=255)
                                               tCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCa                                                                                                       <  2:1795969/150‑1 (MQ=255)
                                                  atcatcGGTAAGGCCCAGGAGACGAAGAAGTTCGCCAGCCACTGTGCCGCCACCGCGATTGCCAGCGCTTTACCACTAATAGCATTCGGGAAGATTTCCGACAGCAGAACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACa                                                                                                    <  1:1070267/150‑1 (MQ=255)
                                                     atcGGGAAGGTCCAGG‑GACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGa                                                                                                <  1:1031533/150‑1 (MQ=255)
                                                       cGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGaa                                                                                               >  2:778128/1‑150 (MQ=255)
                                                          gAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAg                                                                                            <  2:1353913/150‑1 (MQ=255)
                                                           aacgcccAGGAGACGAAGGAGTTAGCCAGCCAGTGGGCCGCCACCGCGAGTGCCAGCGCTTTACCTCGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGGTAAGGGGGCAACATAGAACAGc                                                                                           <  2:1649158/145‑1 (MQ=255)
                                                           aaGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCCTACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGc                                                                                           >  1:1505577/1‑150 (MQ=255)
                                                           aaGGACCAGGAGACGAAGTAGTTCTCCAGCCACTTGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGc                                                                                           <  2:93026/150‑1 (MQ=255)
                                                            aGGTCCATGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCa                                                                                          <  1:1037155/150‑1 (MQ=255)
                                                               tCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGAATCg                                                                                       >  1:512097/1‑150 (MQ=255)
                                                                ccAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGa                                                                                      >  1:1054991/1‑150 (MQ=255)
                                                                 cAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGAc                                                                                     <  1:1718937/150‑1 (MQ=255)
                                                                   ggAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAg                                                                                   <  2:435767/150‑1 (MQ=255)
                                                                   ggAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACAGCGATTGCCAGCGCTTTACCACGAAGAGCATGCGGGCAGCGTTCCGACGGCAGTACCCCGCCTACCGGACACCAGGACATGGCTACGGGGGCCGCAGAGGCCCGCATCGAcgg                                                                                   >  2:1167592/1‑148 (MQ=255)
                                                                     agaCGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCGACATAGATCAGCATCGACAGTa                                                                                 >  1:689843/1‑150 (MQ=255)
                                                                               gtTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAAt                                                                       >  1:845186/1‑150 (MQ=255)
                                                                                  cGCCAGCCACTGGGCCGCCACCGCGTATGCCATCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGCCCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATAcc                                                                    <  2:249983/150‑1 (MQ=255)
                                                                                    ccagccaCTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGGAGCGCCACAATACCCg                                                                  >  1:1778743/1‑150 (MQ=255)
                                                                                       gccaCTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTg                                                               <  1:812087/150‑1 (MQ=255)
                                                                                        ccaCTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGc                                                              >  2:1241581/1‑150 (MQ=255)
                                                                                           cTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGCTTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTg                                                           >  1:46633/1‑150 (MQ=255)
                                                                                           cTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCTGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTg                                                           <  1:1505617/150‑1 (MQ=255)
                                                                                           cTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCTGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTg                                                           <  1:212970/150‑1 (MQ=255)
                                                                                           cTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCTGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTg                                                           <  1:218721/150‑1 (MQ=255)
                                                                                               gccgccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAgtg                                                       <  1:1763872/150‑1 (MQ=255)
                                                                                               gccgccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAgtg                                                       <  2:1060075/150‑1 (MQ=255)
                                                                                               gccgccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAgtg                                                       <  1:1080958/150‑1 (MQ=255)
                                                                                                 cgccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTa                                                     <  1:672426/150‑1 (MQ=255)
                                                                                                  gccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGCCAGCAGTACCCCGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTaa                                                    >  2:1417910/1‑150 (MQ=255)
                                                                                                   cctccGGGATTGCCATCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGTCAGCAGTACCCAGCATACCGGACCCCAGGACATGGCTAAGGCGGCAACATAGTACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTaaa                                                   <  1:1684157/147‑1 (MQ=255)
                                                                                                   ccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACATCAGTACCCAGCATACCTGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTaaa                                                   <  2:16796/150‑1 (MQ=255)
                                                                                                   ccACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTaaa                                                   <  2:1814437/150‑1 (MQ=255)
                                                                                                    cACCGCGATTGCCAGCGCTTTAACTCGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCCCACTACCCGGTGCCTGAGTGGaaaa                                                  >  2:1269273/1‑150 (MQ=255)
                                                                                                     aCCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAAc                                                 <  1:1687074/150‑1 (MQ=255)
                                                                                                      ccGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGTAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAAcg                                                >  1:988209/1‑150 (MQ=255)
                                                                                                       cgcgATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAAcgc                                               <  1:1723433/150‑1 (MQ=255)
                                                                                                       cgcgATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTCCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAAcgc                                               >  1:627692/1‑150 (MQ=255)
                                                                                                        gcgATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAAcgcg                                              <  2:1336688/150‑1 (MQ=255)
                                                                                                        gcgATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGCACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAAcgcg                                              <  2:700701/150‑1 (MQ=255)
                                                                                                         cgATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCgg                                             <  2:668072/150‑1 (MQ=255)
                                                                                                               ccAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGGGCCTGAGTGTAAAACGCGGTACCGa                                       >  2:1428611/1‑150 (MQ=255)
                                                                                                               ccAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCAAACCGGACCCCAGGACATGGAAAAGGCGGCAACATAGAACAGCAGCGACAGAAGCGCCACAATACACGGTTCCTGAGTGTAAACCGCGGTACCgc                                       >  2:728819/1‑149 (MQ=255)
                                                                                                                cAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGGACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAg                                      <  2:387535/150‑1 (MQ=255)
                                                                                                                cAGCGCTTTAACACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAg                                      <  1:893467/150‑1 (MQ=255)
                                                                                                                    gcTTTACCACGAATAGCTTTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGCCCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTa                                  <  1:1626096/150‑1 (MQ=255)
                                                                                                                       ttACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACAGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAAc                               <  1:649112/150‑1 (MQ=255)
                                                                                                                         aCCACGAATAGCATTCGGGAAGATTTCCGACAGAAGTACCCAGCATACCGGACCACAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTCCCGAGGCTAAACaa                             >  1:772403/1‑149 (MQ=255)
                                                                                                                          ccACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGCACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTCCCGAGGCTAAACATa                            >  1:883200/1‑150 (MQ=255)
                                                                                                                          ccACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATa                            >  2:1414643/1‑150 (MQ=255)
                                                                                                                            ccGAATAGCATTCGGGAAGATATCCGACAGCAGTACCCCGCATACCGGCCCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATAcc                          <  1:1731782/149‑1 (MQ=255)
                                                                                                                            aCGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACTTGGCAAAGGTGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATAcc                          <  2:843685/150‑1 (MQ=255)
                                                                                                                                      ttCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACGTACCGATTGCCAtt                <  2:1546105/150‑1 (MQ=255)
                                                                                                                                        cGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCCTTcc              >  1:456586/1‑150 (MQ=255)
                                                                                                                                        cGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTcc              >  2:616838/1‑150 (MQ=255)
                                                                                                                                        cGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTcc              <  2:1329320/150‑1 (MQ=255)
                                                                                                                                         gggAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAAAACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCg             >  1:1103387/1‑150 (MQ=255)
                                                                                                                                          ggAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGa            >  2:1052704/1‑150 (MQ=255)
                                                                                                                                           gAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCAGCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAg           <  1:1396423/150‑1 (MQ=255)
                                                                                                                                            aaGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGt          <  1:1584020/150‑1 (MQ=255)
                                                                                                                                               aTTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTgcg       <  1:82304/150‑1 (MQ=255)
                                                                                                                                                    cGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGAt  <  2:620411/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGACAGTAGCGCCACAATACCCGGTGCCTGAGTGTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGAT  >  NC_000913/4240919‑4241216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑