Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,245,108 T→G 6.1% intergenic (‑33/+121) malF ← / ← malE maltose transporter subunit/maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,245,1080TG 6.1% 440.7 / 14.9 147intergenic (‑33/+121)malF/malEmaltose transporter subunit/maltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (79/59);  new base G (6/3);  total (85/62)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.83e-01

Only 100 of 148 total aligned reads displayed.

CCCTTGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTT  >  NC_000913/4244962‑4245257
                                                                                                                                                  |                                                                                                                                                     
cccTTGTGCGTACATTAAAACAACAAGGTAACCCACCAGCCGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATACGGCTTTc                                                                                                                                                    >  2:872011/1‑150 (MQ=255)
cccTTGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTc                                                                                                                                                    <  1:1793717/150‑1 (MQ=255)
   ttGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACg                                                                                                                                                 <  2:102587/150‑1 (MQ=255)
      tgCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCt                                                                                                                                              >  2:572663/1‑150 (MQ=255)
      tgCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCt                                                                                                                                              >  2:12915/1‑150 (MQ=255)
       gCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGCGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACCCCtt                                                                                                                                             >  2:1518055/1‑150 (MQ=255)
        cGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTa                                                                                                                                            >  2:431882/1‑150 (MQ=255)
          tACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                          <  2:452555/150‑1 (MQ=255)
          tACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                          >  2:1473351/1‑150 (MQ=255)
          tACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                          >  2:1572415/1‑150 (MQ=255)
          tACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACAATTTCAGCGCGTCGCTATTCCACCAATGATTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                          >  1:1012331/1‑150 (MQ=255)
            cATTAAAACAACCAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCg                                                                                                                                        <  1:882228/150‑1 (MQ=255)
            cATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCg                                                                                                                                        >  2:1660196/1‑150 (MQ=255)
             aTTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                       >  1:536267/1‑150 (MQ=255)
             aTTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGAGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTATCCgt                                                                                                                                       <  1:343457/150‑2 (MQ=255)
              ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGac                                                                                                                                      >  2:288255/1‑150 (MQ=255)
              ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGac                                                                                                                                      >  1:1680931/1‑150 (MQ=255)
               tAAAACAACCAGGTAACCCACCAGCAGGCCGAGAAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTGCCTCATTCCAGGACGGATAAGGCTTTCACTCCTTATCCGaca                                                                                                                                     >  1:732485/1‑150 (MQ=255)
               tAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGaca                                                                                                                                     <  1:361784/150‑1 (MQ=255)
               tAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGaca                                                                                                                                     >  2:609608/1‑150 (MQ=255)
                aaaaCAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGacaa                                                                                                                                    <  1:471235/150‑1 (MQ=255)
                aaaaCAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGacaa                                                                                                                                    >  2:565914/1‑150 (MQ=255)
                  aacaacAATGTAACCCACCAGCATGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGacaaca                                                                                                                                  <  2:1246819/150‑1 (MQ=255)
                  aacaacAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGacacca                                                                                                                                  >  2:96642/1‑150 (MQ=255)
                      acaaGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCATCATTACAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTg                                                                                                                              >  2:1386957/1‑150 (MQ=255)
                        aaGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCTTTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGcc                                                                                                                            <  2:1011901/150‑1 (MQ=255)
                         tgttAACCCACCAGCAGGCCTAGCATACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCAACCATTGTTTATTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCt                                                                                                                           <  1:1329386/147‑1 (MQ=255)
                         aGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCt                                                                                                                           >  1:1781575/1‑150 (MQ=255)
                               cccACCAGCATGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACACCTGCCTGATGcc                                                                                                                     >  1:1343183/1‑149 (MQ=255)
                                ccaccaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACTAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGa                                                                                                                    >  2:193670/1‑150 (MQ=255)
                                ccaccaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGa                                                                                                                    >  2:1706507/1‑150 (MQ=255)
                                 caccaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGATTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGAc                                                                                                                   <  2:1223047/150‑1 (MQ=255)
                                   ccaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGc                                                                                                                 >  1:1443316/1‑150 (MQ=255)
                                     agcagGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCATCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGACTGATGCGACGCTg                                                                                                               >  2:966697/1‑150 (MQ=255)
                                       cagGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAc                                                                                                             >  1:616603/1‑150 (MQ=255)
                                       cagGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAc                                                                                                             >  1:506519/1‑150 (MQ=255)
                                         ggCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgc                                                                                                           >  2:1059424/1‑150 (MQ=255)
                                          gCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgcg                                                                                                          <  1:1272923/150‑1 (MQ=255)
                                          gCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgcg                                                                                                          <  1:162926/150‑1 (MQ=255)
                                                   aCCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg                                                                                                 >  2:371133/1‑150 (MQ=255)
                                                     cTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGc                                                                                               <  2:353501/150‑1 (MQ=255)
                                                     cTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGc                                                                                               <  2:1290229/150‑1 (MQ=255)
                                                      tAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGcc                                                                                              <  2:314625/150‑1 (MQ=255)
                                                                cATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGtt                                                                                    <  2:151036/150‑1 (MQ=255)
                                                                   ttCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCg                                                                                 <  2:1825243/150‑1 (MQ=255)
                                                                         gcgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTTACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGTttttt                                                                           >  1:32844/1‑149 (MQ=255)
                                                                         gcgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTg                                                                           <  1:1572415/150‑1 (MQ=255)
                                                                           gTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTa                                                                         >  1:501170/1‑150 (MQ=255)
                                                                                tttGCCAGCTATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATATGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCg                                                                    <  1:1518055/150‑1 (MQ=255)
                                                                                tttGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTAATTCCAGGACGTATAAGGCTTTCACCCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCg                                                                    <  2:1661308/150‑1 (MQ=255)
                                                                                 ttGCCACCAATGTTTCTTTTTAATGACATCCATGGGGGTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCgg                                                                   <  1:246381/150‑1 (MQ=255)
                                                                                     caccaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACaa                                                               >  1:980651/1‑150 (MQ=255)
                                                                                     caccaAAGTTTCTTTTTAATGAACTCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACTCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACaa                                                               <  2:885510/150‑1 (MQ=255)
                                                                                          aTGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCAATCCAGGACGGATAATGCTTTCACGCCTTTTCCGACAACAACTGCCTGATGCGTCGCTGACGAGTCTTATCAGGCGTACATTCTTTGCGGTTTTGTAGGCCGGACAAGGCGt                                                          <  1:1620332/150‑1 (MQ=255)
                                                                                                cTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTc                                                        <  1:136694/146‑1 (MQ=255)
                                                                                                cTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTc                                                        >  2:136694/1‑146 (MQ=255)
                                                                                                    ttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCa                                                >  1:833681/1‑150 (MQ=255)
                                                                                                          aCATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGc                                          >  2:1010787/1‑150 (MQ=255)
                                                                                                           cATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGAGGCGGCGTGAACGCCTTGTCCGGcc                                         <  1:1690750/150‑2 (MQ=37)
                                                                                                            aTCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGATGCGGCGTGAACGCCTTGTCCGGcct                                        <  2:1680931/150‑3 (MQ=37)
                                                                                                             tCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCAtt                                       <  1:1706507/150‑1 (MQ=255)
                                                                                                             tCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCAtt                                       >  1:1278732/1‑150 (MQ=255)
                                                                                                             tCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCAtt                                       <  1:1718761/150‑1 (MQ=255)
                                                                                                             tCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCAtt                                       <  1:371133/150‑1 (MQ=255)
                                                                                                               cATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTc                                     >  1:1212955/1‑150 (MQ=255)
                                                                                                               cATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTc                                     <  2:455335/150‑1 (MQ=255)
                                                                                                                 tGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTcac                                   <  1:572663/150‑1 (MQ=255)
                                                                                                                   gggTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAg                                 <  1:288255/150‑1 (MQ=255)
                                                                                                                   gggTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAg                                 <  1:12915/150‑1 (MQ=255)
                                                                                                                    ggTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGc                                <  1:1305644/150‑1 (MQ=255)
                                                                                                                    ggTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGc                                >  2:345386/1‑150 (MQ=255)
                                                                                                                     gTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCa                               >  1:23368/1‑150 (MQ=255)
                                                                                                                       tcttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCAtt                             <  2:1456945/150‑1 (MQ=255)
                                                                                                                          tcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGTCAACAACTGCCTGATGCGTCGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACt                          >  2:236251/1‑150 (MQ=255)
                                                                                                                          tcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACt                          >  2:676894/1‑150 (MQ=255)
                                                                                                                           ccTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACtt                         >  2:700467/1‑150 (MQ=255)
                                                                                                                           ccTCATTCCAGGACGGATAAGGATTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTATTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACtt                         >  1:982362/1‑150 (MQ=255)
                                                                                                                             tCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTgg                       <  1:431882/150‑1 (MQ=255)
                                                                                                                              cATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGt                      <  2:1209885/150‑1 (MQ=255)
                                                                                                                              cATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGt                      >  2:837016/1‑150 (MQ=255)
                                                                                                                              cATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGt                      >  2:841983/1‑150 (MQ=255)
                                                                                                                              aaTTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGGCAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGt                      <  2:1112182/149‑1 (MQ=255)
                                                                                                                               aTTCCCGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGACCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTg                     <  2:955762/150‑1 (MQ=255)
                                                                                                                               aTTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTg                     <  2:732487/150‑1 (MQ=255)
                                                                                                                                 tCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGAt                   <  2:1029321/150‑1 (MQ=255)
                                                                                                                                   cAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACCAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATAc                 >  1:746653/1‑150 (MQ=255)
                                                                                                                                   cAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATAc                 >  2:592315/1‑150 (MQ=255)
                                                                                                                                   cAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATAc                 >  2:671291/1‑150 (MQ=255)
                                                                                                                                    aGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACg                >  2:348939/1‑150 (MQ=255)
                                                                                                                                        cGGATAAGGCGTGAAAGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTc            >  2:939869/1‑150 (MQ=255)
                                                                                                                                        cGGATAAGGCGTGAAAGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTc            >  2:229722/1‑150 (MQ=255)
                                                                                                                                        cGGATAAGGCGTGAAAGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTc            >  1:1198382/1‑150 (MQ=255)
                                                                                                                                         ggATAAGGCGTGAAAGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCt           >  1:733145/1‑150 (MQ=255)
                                                                                                                                         ggATAAGGCGTGAAAGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCt           >  1:1471664/1‑150 (MQ=255)
                                                                                                                                              aGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCTCGCCGCCTCCGGCATTTCACAGCATTACTTGGTGCTTCGTGTCTGcgcg      >  2:755365/1‑150 (MQ=38)
                                                                                                                                              aGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGcgcg      <  1:1473351/150‑1 (MQ=255)
                                                                                                                                                gCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTc    >  2:1563171/1‑150 (MQ=255)
                                                                                                                                                gCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTc    >  1:1123199/1‑150 (MQ=255)
                                                                                                                                                gCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTc    <  1:1059424/150‑1 (MQ=255)
                                                                                                                                                  tttCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCtt  <  2:720302/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CCCTTGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTT  >  NC_000913/4244962‑4245257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: