Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,281,679 T→G 5.4% intergenic (+50/+104) yjcE → / ← yjcF putative cation/proton antiporter/pentapeptide repeats protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,281,6790TG 5.4% 451.4 / 12.9 150intergenic (+50/+104)yjcE/yjcFputative cation/proton antiporter/pentapeptide repeats protein
Reads supporting (aligned to +/- strand):  ref base T (70/72);  new base G (5/3);  total (75/75)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01

Only 100 of 153 total aligned reads displayed.

CCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGAC  >  NC_000913/4281530‑4281827
                                                                                                                                                     |                                                                                                                                                    
ccaccTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCt                                                                                                                                                      <  2:1497650/150‑1 (MQ=255)
  cccTGCGCGCCACACGGGAGATCAGCAACGAAACGCTTCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCttt                                                                                                                                                    <  1:857280/149‑1 (MQ=255)
  accTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGCTCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCttt                                                                                                                                                    >  1:250978/1‑150 (MQ=255)
         cgcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCt                                                                                                                                             >  2:605611/1‑150 (MQ=255)
         cgcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGAAGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCt                                                                                                                                             >  1:122055/1‑150 (MQ=255)
         cgcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGAGCTCGATTTGCTTGAAGCGTAGCTAATTGAGGAAAATCAGTAAGCCTAAAGCGACTTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGAACCt                                                                                                                                             >  1:280994/1‑150 (MQ=255)
          gcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCtt                                                                                                                                            >  2:192019/1‑150 (MQ=255)
           ccACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGAACGAGCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACAAGGCCCGAGGTAATCTGAAAACGGTGCAATCTTTTGCACCttt                                                                                                                                           >  2:883384/1‑150 (MQ=255)
           ccACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACATCCCGATCTCGATTTGCTTGAAGCGGGGCTAAGAGAGGACAATCAGTAACCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGGGCAATCTTTTGCACCatt                                                                                                                                           >  1:328061/1‑150 (MQ=255)
              aacGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTTATCTGAAAAAGGTGCAAAAGATTGCACCtttttc                                                                                                                                        <  1:231826/149‑4 (MQ=255)
               acGTGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACa                                                                                                                                       <  1:1594570/150‑1 (MQ=255)
               acGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCGCGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACa                                                                                                                                       <  1:686473/150‑1 (MQ=255)
               acGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACa                                                                                                                                       >  2:788097/1‑150 (MQ=255)
                 gggAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTACTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAgg                                                                                                                                     <  2:1317111/150‑1 (MQ=255)
                     taTCAGCAACGAAACGCTGCAAAAATTACTTCACTATCTCGATTTCCTTGAAGCGTTGCTAATTGACGAAAATCACTAAGCCGAAAGCCACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAg                                                                                                                                 <  1:1653513/149‑1 (MQ=255)
                        cAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTc                                                                                                                              >  1:789066/1‑150 (MQ=255)
                          gCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCgg                                                                                                                            >  1:760404/1‑150 (MQ=255)
                            aaCGAAACGCTGCAAAAATTACTGCACGAGCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGc                                                                                                                          <  1:208600/150‑1 (MQ=255)
                              cGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTa                                                                                                                        <  2:1569165/150‑1 (MQ=255)
                                aaaCGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAAc                                                                                                                      <  1:1489214/150‑1 (MQ=255)
                                 aaCGCTGCAAAAATTACTGCACGATGTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACa                                                                                                                     <  2:1506394/150‑1 (MQ=255)
                                 aaCGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACa                                                                                                                     <  1:1567103/150‑1 (MQ=255)
                                     cTGAAAAAATTAATGCACGAACTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGTCGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCa                                                                                                                 <  1:1645875/150‑1 (MQ=255)
                                      tGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGATAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCAt                                                                                                                <  1:1288097/150‑1 (MQ=255)
                                       gCAAAAATTACTGCACTATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGATGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATg                                                                                                               <  1:1111994/150‑1 (MQ=255)
                                       gCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGTAAATCAGTATGCCGAAAGCGACGTAACCAGGCCAGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATg                                                                                                               <  2:878025/150‑1 (MQ=255)
                                       gCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAATATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCCCCTTTAACAGGTTAGCTCGGGCTAACATTCATg                                                                                                               >  2:1064185/1‑150 (MQ=255)
                                       gCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATg                                                                                                               <  1:762881/150‑1 (MQ=255)
                                       gCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATg                                                                                                               <  2:1812690/150‑1 (MQ=255)
                                        cAAAAATTACTGCACGATCTCGATTTGCTTGACGCGTTGCTAATTGAGGGAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCCCCTTTAACAGGTTAGCTCGGGCTAACATTCATGa                                                                                                              >  2:339047/1‑150 (MQ=255)
                                        cAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGa                                                                                                              >  2:608665/1‑150 (MQ=255)
                                          aaaaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGAtt                                                                                                            >  1:558655/1‑150 (MQ=255)
                                           aaaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGGAAGCCGAATGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTc                                                                                                           <  1:959964/150‑1 (MQ=255)
                                            aaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCt                                                                                                          <  2:521712/150‑1 (MQ=255)
                                            aaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCt                                                                                                          <  2:1277672/150‑1 (MQ=255)
                                                aCTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGGCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATTATTCTaaaa                                                                                                      >  1:1122944/1‑150 (MQ=255)
                                                   gCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaa                                                                                                   <  2:1276845/150‑1 (MQ=255)
                                                      cGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaaata                                                                                                >  2:493465/1‑150 (MQ=255)
                                                        aTCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGTCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaaataaa                                                                                              <  1:24666/150‑1 (MQ=255)
                                                        aTCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaaataaa                                                                                              >  2:823993/1‑150 (MQ=255)
                                                              aTTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGATGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTAtct                                                                                        >  1:1593247/1‑150 (MQ=255)
                                                                 tGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTAtctctc                                                                                     >  1:1000692/1‑150 (MQ=255)
                                                                  gCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATctctct                                                                                    >  1:601507/1‑150 (MQ=255)
                                                                   cTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCtt                                                                                   <  2:1024395/150‑1 (MQ=255)
                                                                    ttGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCttt                                                                                  >  2:682812/1‑150 (MQ=255)
                                                                    ttGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCttt                                                                                  <  2:1190861/150‑1 (MQ=255)
                                                                       aaGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTaa                                                                               >  1:1708905/1‑150 (MQ=255)
                                                                        aGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTaaa                                                                              >  2:892344/1‑150 (MQ=255)
                                                                        aGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTATTTTaaa                                                                              >  2:886376/1‑150 (MQ=255)
                                                                         gCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAg                                                                             <  2:322283/150‑1 (MQ=255)
                                                                           gTTGATAATTGAGGAAATTCAGTAAGCCGAAAGCGACGTAACCAGGCCCGATGTAATCTGAAAAAGGTGCTATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTc                                                                           <  2:936872/150‑1 (MQ=255)
                                                                                 aaTTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCa                                                                     >  2:1562796/1‑150 (MQ=255)
                                                                                   ttGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGAAATATTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTAATGATTCTAAAACAAAGTAATTATCTATCTTTTAAAGTCAATTCAtt                                                                   >  1:89302/1‑150 (MQ=255)
                                                                                    tGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTg                                                                  >  1:10129/1‑150 (MQ=255)
                                                                                    tGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTg                                                                  <  2:1682568/150‑1 (MQ=255)
                                                                                      aGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAGAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGc                                                                >  2:1533250/1‑150 (MQ=255)
                                                                                      aGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTgga                                                                >  2:1210171/1‑149 (MQ=255)
                                                                                      aGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGc                                                                <  2:689433/150‑1 (MQ=255)
                                                                                      aGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGc                                                                <  2:766453/150‑1 (MQ=255)
                                                                                       ggAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCt                                                               >  2:112124/1‑150 (MQ=255)
                                                                                            aTCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGttct                                                          <  2:1599405/150‑1 (MQ=255)
                                                                                             tCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGttctt                                                         <  2:259410/150‑1 (MQ=255)
                                                                                               aGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCATTTCATTGGCTGTTCTTCg                                                       >  2:459520/1‑150 (MQ=255)
                                                                                                  aaGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACt                                                    >  1:1702931/1‑150 (MQ=255)
                                                                                                    gCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTACTTCGACTTa                                                  <  2:760404/150‑1 (MQ=255)
                                                                                                     ccGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTaa                                                 >  2:1532696/1‑150 (MQ=255)
                                                                                                            cGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAAt                                          >  2:105051/1‑150 (MQ=255)
                                                                                                              aagTAACCAGGCCCGAGGTAATCTGAAAAATGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCt                                        <  2:1287139/148‑1 (MQ=255)
                                                                                                              aCGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCt                                        >  1:253675/1‑150 (MQ=255)
                                                                                                               cGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTg                                       <  2:754552/150‑1 (MQ=255)
                                                                                                                ttAACCAGGCCCGAGGTAATATGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGc                                      <  1:1371410/149‑1 (MQ=255)
                                                                                                                gTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGc                                      <  1:1262978/150‑1 (MQ=255)
                                                                                                                 tAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCa                                     >  1:73434/1‑150 (MQ=255)
                                                                                                                    ccAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATGTCTCTTTTAAAGTCAATTCATTGGATGTTCTGCGACTTAACCTCAATCTGCATCt                                  >  2:282872/1‑150 (MQ=255)
                                                                                                                    ccAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCt                                  >  1:520753/1‑150 (MQ=255)
                                                                                                                     cAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCtt                                 >  1:445484/1‑150 (MQ=255)
                                                                                                                       ggCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGc                               >  1:1536480/1‑150 (MQ=255)
                                                                                                                         cccGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCat                             <  2:355787/150‑1 (MQ=255)
                                                                                                                          ccGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGTCTGTTCTTCGACTTAACCTCAATCTGCATATTGCata                            >  1:1204136/1‑150 (MQ=255)
                                                                                                                           cGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCataa                           <  1:451481/150‑1 (MQ=255)
                                                                                                                               gTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATc                       <  1:1158496/150‑1 (MQ=255)
                                                                                                                                tAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAAACTCAATCTGCATCTTGCATAATATCt                      >  1:1170320/1‑150 (MQ=255)
                                                                                                                                  acctgttaaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGc                    >  2:559562/8‑150 (MQ=39)
                                                                                                                                    cTGAATAAGGTGCAATCTTTTGCAGCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACATATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAg                  <  2:449265/150‑1 (MQ=255)
                                                                                                                                     tGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGt                 >  2:768398/1‑150 (MQ=255)
                                                                                                                                     tGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGt                 >  2:1610291/1‑150 (MQ=255)
                                                                                                                                        taaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAAt              >  2:1122909/2‑150 (MQ=255)
                                                                                                                                        taaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAAt              >  2:1016951/2‑150 (MQ=255)
                                                                                                                                        taaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAAt              >  1:1331915/2‑150 (MQ=255)
                                                                                                                                         aaaGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATc             <  1:33332/150‑1 (MQ=255)
                                                                                                                                         aaaGGTGCAATCTTTTGAACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATc             <  1:823993/150‑1 (MQ=255)
                                                                                                                                            ggTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCtta          >  1:1374753/1‑149 (MQ=255)
                                                                                                                                            ggTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTc          <  1:1243935/150‑1 (MQ=255)
                                                                                                                                             gTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCa         <  1:382595/150‑1 (MQ=255)
                                                                                                                                              tGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCCTCaa        >  1:565729/1‑149 (MQ=255)
                                                                                                                                               gCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATa       >  1:607092/1‑150 (MQ=255)
                                                                                                                                                 aaTCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGc     >  1:1081253/1‑150 (MQ=255)
                                                                                                                                                 aaTCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTATCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGc     >  1:514432/1‑150 (MQ=255)
                                                                                                                                                  aTCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCg    <  1:1068154/150‑1 (MQ=255)
                                                                                                                                                    cTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAACACAAAAAAATTATATCTCTTTTAAAGTCAATTCATTTGCTGTTCTTCGCCTTAACCTCAATCTGCATCTTGAATAATCTCTGCAGTAATCTTCATAGCGAc  <  2:328061/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGAC  >  NC_000913/4281530‑4281827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: