Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,281,680 T→A 5.3% intergenic (+51/+103) yjcE → / ← yjcF putative cation/proton antiporter/pentapeptide repeats protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,281,6800TA 5.3% 449.0 / 9.3 150intergenic (+51/+103)yjcE/yjcFputative cation/proton antiporter/pentapeptide repeats protein
Reads supporting (aligned to +/- strand):  ref base T (70/72);  new base A (5/3);  total (75/75)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01

Only 100 of 152 total aligned reads displayed.

ACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGAC  >  NC_000913/4281532‑4281827
                                                                                                                                                     |                                                                                                                                                   
accTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGCTCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCttt                                                                                                                                                    >  1:250978/1‑150 (MQ=255)
  cTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTg                                                                                                                                                  <  1:526107/150‑1 (MQ=255)
   tGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAATTCAGTAAGCCTAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGc                                                                                                                                                 <  2:1148760/150‑1 (MQ=255)
       cgcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCt                                                                                                                                             >  2:605611/1‑150 (MQ=255)
       cgcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGAAGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCt                                                                                                                                             >  1:122055/1‑150 (MQ=255)
       cgcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGAGCTCGATTTGCTTGAAGCGTAGCTAATTGAGGAAAATCAGTAAGCCTAAAGCGACTTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGAACCt                                                                                                                                             >  1:280994/1‑150 (MQ=255)
        gcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCtt                                                                                                                                            >  2:192019/1‑150 (MQ=255)
         ccACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGCTAATCTGAAAAAGGTGCAATCTTTTGCACCttt                                                                                                                                           >  2:476013/1‑150 (MQ=255)
         ccACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGAACGAGCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACAAGGCCCGAGGTAATCTGAAAACGGTGCAATCTTTTGCACCttt                                                                                                                                           >  2:883384/1‑150 (MQ=255)
         ccACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACATCCCGATCTCGATTTGCTTGAAGCGGGGCTAAGAGAGGACAATCAGTAACCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGGGCAATCTTTTGCACCatt                                                                                                                                           >  1:328061/1‑150 (MQ=255)
            aacGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTTATCTGAAAAAGGTGCAAAAGATTGCACCtttttc                                                                                                                                        <  1:231826/149‑4 (MQ=255)
             acGTGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACa                                                                                                                                       <  1:1594570/150‑1 (MQ=255)
             acGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACa                                                                                                                                       >  2:788097/1‑150 (MQ=255)
              cGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAg                                                                                                                                      >  1:457802/1‑150 (MQ=255)
               gggAGATCAGCAACGAAACGCTGCTAAAATTAATGCACGATCTCGATTTGCTTTAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAACAGGTGCAATCTTTTGCACCTTTAACAgg                                                                                                                                     <  1:1575348/150‑1 (MQ=255)
               gggAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTACTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAgg                                                                                                                                     <  2:1317111/150‑1 (MQ=255)
                 gagaTCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGtt                                                                                                                                   >  2:382595/1‑150 (MQ=255)
                   taTCAGCAACGAAACGCTGCAAAAATTACTTCACTATCTCGATTTCCTTGAAGCGTTGCTAATTGACGAAAATCACTAAGCCGAAAGCCACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAg                                                                                                                                 <  1:1653513/149‑1 (MQ=255)
                        gCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCgg                                                                                                                            >  1:579970/1‑150 (MQ=255)
                          aaCGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGc                                                                                                                          >  1:416938/1‑150 (MQ=255)
                          aaCGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGc                                                                                                                          <  1:1609173/150‑1 (MQ=255)
                          aaCGAAACGCTGCAAAAATTACTGCACGAGCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGc                                                                                                                          <  1:208600/150‑1 (MQ=255)
                            cGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTa                                                                                                                        <  2:1569165/150‑1 (MQ=255)
                              aaaCGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAAc                                                                                                                      <  1:1489214/150‑1 (MQ=255)
                               aaCGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACa                                                                                                                     <  1:1567103/150‑1 (MQ=255)
                                   cTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAGAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTTAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCCCGAGCTAAcctgtt                                                                                                                 <  2:1219340/150‑6 (MQ=255)
                                    tGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCa                                                                                                                 <  2:1039187/150‑1 (MQ=255)
                                    tGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGATAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCAt                                                                                                                <  1:1288097/150‑1 (MQ=255)
                                     gCAAAAATTACTGCACTATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGATGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATg                                                                                                               <  1:1111994/150‑1 (MQ=255)
                                     gCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGTAAATCAGTATGCCGAAAGCGACGTAACCAGGCCAGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATg                                                                                                               <  2:878025/150‑1 (MQ=255)
                                     gCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAATATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCCCCTTTAACAGGTTAGCTCGGGCTAACATTCATg                                                                                                               >  2:1064185/1‑150 (MQ=255)
                                     gCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATg                                                                                                               <  1:762881/150‑1 (MQ=255)
                                     gCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATg                                                                                                               <  2:1812690/150‑1 (MQ=255)
                                      cAAAAATTACTGCACGATCTCGATTTGCTTGACGCGTTGCTAATTGAGGGAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCCCCTTTAACAGGTTAGCTCGGGCTAACATTCATGa                                                                                                              >  2:339047/1‑150 (MQ=255)
                                      cAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGa                                                                                                              >  2:608665/1‑150 (MQ=255)
                                        aaaaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATCAtt                                                                                                            >  1:1695358/1‑150 (MQ=255)
                                         aaaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGGAAGCCGAATGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTc                                                                                                           <  1:959964/150‑1 (MQ=255)
                                          aaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCt                                                                                                          <  2:1277672/150‑1 (MQ=255)
                                             tACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAACGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTaaa                                                                                                       <  1:628190/150‑1 (MQ=255)
                                              aCTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGGCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATTATTCTaaaa                                                                                                      >  1:1122944/1‑150 (MQ=255)
                                               cTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAAc                                                                                                     <  2:12826/150‑1 (MQ=255)
                                                    cGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaaata                                                                                                >  2:493465/1‑150 (MQ=255)
                                                      aTCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGTCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaaataaa                                                                                              <  1:24666/150‑1 (MQ=255)
                                                      aTCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaaataaa                                                                                              >  1:966154/1‑150 (MQ=255)
                                                      aTCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaaataaa                                                                                              >  2:33332/1‑150 (MQ=255)
                                                      aTCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaaataaa                                                                                              >  2:823993/1‑150 (MQ=255)
                                                        ctcGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAAtt                                                                                            <  2:1353275/150‑1 (MQ=255)
                                                             tttGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTAtctc                                                                                       <  1:751570/150‑1 (MQ=255)
                                                               tGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTAtctctc                                                                                     >  1:1000692/1‑150 (MQ=255)
                                                                 cTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCtt                                                                                   <  2:1024395/150‑1 (MQ=255)
                                                                  ttGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCttt                                                                                  >  2:682812/1‑150 (MQ=255)
                                                                     aaGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTaa                                                                               >  1:1708905/1‑150 (MQ=255)
                                                                      aGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTaaa                                                                              >  2:892344/1‑150 (MQ=255)
                                                                      aGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTATTTTaaa                                                                              >  2:886376/1‑150 (MQ=255)
                                                                       gCGTTGCTAATTGAGGAAAATCAGTAATCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAg                                                                             <  2:322070/150‑1 (MQ=255)
                                                                       gCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAg                                                                             <  2:322283/150‑1 (MQ=255)
                                                                        cGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGt                                                                            <  2:1523052/150‑1 (MQ=255)
                                                                         gTTGATAATTGAGGAAATTCAGTAAGCCGAAAGCGACGTAACCAGGCCCGATGTAATCTGAAAAAGGTGCTATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTc                                                                           <  2:936872/150‑1 (MQ=255)
                                                                           tGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCaa                                                                         >  2:1110370/1‑150 (MQ=255)
                                                                               aaTTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCa                                                                     >  2:1562796/1‑150 (MQ=255)
                                                                               aaTTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCa                                                                     <  2:558655/150‑1 (MQ=255)
                                                                                 ttGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGAAATATTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTAATGATTCTAAAACAAAGTAATTATCTATCTTTTAAAGTCAATTCAtt                                                                   >  1:89302/1‑150 (MQ=255)
                                                                                  tGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTg                                                                  >  1:10129/1‑150 (MQ=255)
                                                                                  tGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTg                                                                  <  2:1682568/150‑1 (MQ=255)
                                                                                    aGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTgga                                                                >  2:1210171/1‑149 (MQ=255)
                                                                                    aGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGc                                                                <  2:766453/150‑1 (MQ=255)
                                                                                    aGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGc                                                                <  2:689433/150‑1 (MQ=255)
                                                                                        aaaTCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAATCAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGtt                                                            >  1:832906/1‑150 (MQ=255)
                                                                                          aTCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGttct                                                          <  2:1599405/150‑1 (MQ=255)
                                                                                           tCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGttctt                                                         <  2:259410/150‑1 (MQ=255)
                                                                                                  gCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTACTTCGACTTa                                                  <  2:760404/150‑1 (MQ=255)
                                                                                                   ccGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTaa                                                 >  2:1532696/1‑150 (MQ=255)
                                                                                                            aCGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCt                                        >  1:253675/1‑150 (MQ=255)
                                                                                                             cGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTg                                       <  2:754552/150‑1 (MQ=255)
                                                                                                              ttAACCAGGCCCGAGGTAATATGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGc                                      <  1:1371410/149‑1 (MQ=255)
                                                                                                              gTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGc                                      <  1:1262978/150‑1 (MQ=255)
                                                                                                               tAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCa                                     >  1:73434/1‑150 (MQ=255)
                                                                                                                 aCCAGGCCCGAGGTAATCTGATAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATc                                   <  1:1526426/150‑1 (MQ=255)
                                                                                                                  ccAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCt                                  >  1:520753/1‑150 (MQ=255)
                                                                                                                        ccGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGTCTGTTCTTCGACTTAACCTCAATCTGCATATTGCata                            >  1:1204136/1‑150 (MQ=255)
                                                                                                                         cGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCataa                           <  1:451481/150‑1 (MQ=255)
                                                                                                                         cGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCataa                           >  2:154899/1‑150 (MQ=255)
                                                                                                                             gTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATc                       <  1:1158496/150‑1 (MQ=255)
                                                                                                                              tAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAAACTCAATCTGCATCTTGCATAATATCt                      >  1:1170320/1‑150 (MQ=255)
                                                                                                                                acctgttaaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGc                    >  2:559562/8‑150 (MQ=39)
                                                                                                                                aTCTGAAAAAGGTGCAATCTTTTGCAACTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGc                    <  1:788097/150‑1 (MQ=255)
                                                                                                                                  cTGAATAAGGTGCAATCTTTTGCAGCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACATATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAg                  <  2:449265/150‑1 (MQ=255)
                                                                                                                                   tGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGt                 >  2:768398/1‑150 (MQ=255)
                                                                                                                                   tGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGt                 >  2:1610291/1‑150 (MQ=255)
                                                                                                                                      taaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAAt              >  2:1122909/2‑150 (MQ=255)
                                                                                                                                      taaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAAt              >  1:1331915/2‑150 (MQ=255)
                                                                                                                                       aaaGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATc             <  1:33332/150‑1 (MQ=255)
                                                                                                                                       aaaGGTGCAATCTTTTGAACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATc             <  1:823993/150‑1 (MQ=255)
                                                                                                                                           ggTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCtta          >  1:1374753/1‑149 (MQ=255)
                                                                                                                                           ggTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTc          <  1:1243935/150‑1 (MQ=255)
                                                                                                                                            gTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCa         <  1:382595/150‑1 (MQ=255)
                                                                                                                                             tGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCCTCaa        >  1:565729/1‑149 (MQ=255)
                                                                                                                                              gCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATa       >  1:607092/1‑150 (MQ=255)
                                                                                                                                                aaTCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGc     >  1:1081253/1‑150 (MQ=255)
                                                                                                                                                 aTCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCg    <  1:1068154/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGAC  >  NC_000913/4281532‑4281827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: