Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,307,298 G→A 8.9% P168S (CCC→TCC)  alsK ← D‑allose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,307,2980GA 8.9% 250.8 / 10.7 90P168S (CCC→TCC) alsKD‑allose kinase
Reads supporting (aligned to +/- strand):  ref base G (40/42);  new base A (5/3);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01

AGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGA  >  NC_000913/4307149‑4307441
                                                                                                                                                     |                                                                                                                                               
aGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTGTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAgggg                                                                                                                                                 <  1:67329/150‑1 (MQ=255)
 ggggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAggggg                                                                                                                                                <  1:836148/150‑1 (MQ=255)
 ggggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAggggg                                                                                                                                                <  2:1547929/150‑1 (MQ=255)
 ggggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAggggg                                                                                                                                                <  2:1741294/150‑1 (MQ=255)
 ggggCGGTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTTCCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAggggg                                                                                                                                                <  2:846948/150‑1 (MQ=255)
  gggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGa                                                                                                                                               >  2:379164/1‑150 (MQ=255)
  gggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGa                                                                                                                                               <  2:1712795/150‑1 (MQ=255)
  gggCGGTTTCCGCATGGACGACAAGCTCGCGCAATGGGGAATTTCGGGGCTGTTGTTCGTACCAGCGTCGTAGCGCCATTCCAGAGCAATTGGTTGCCCGGCACCCAGGATTGCCACACGCGCAGTGTGGGGTCATATCTCCCAGGGGGa                                                                                                                                               <  1:1766436/150‑1 (MQ=255)
   ggCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGGTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGat                                                                                                                                              <  1:1133333/150‑1 (MQ=255)
    gCGTTTTCCGCATGGACGAAAAGATCTCTCAATGGGTACTTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGACATTCCAGAGCAATTGGTTTCCACGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGata                                                                                                                                             <  2:243066/150‑1 (MQ=255)
      gTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATg                                                                                                                                           >  2:387899/1‑150 (MQ=255)
        tttCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAc                                                                                                                                         <  1:390105/150‑1 (MQ=255)
         ttCCGCATGGACGACAAGATCGCGCAATGGGGAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAcc                                                                                                                                        <  2:1147160/150‑1 (MQ=255)
         ttCCGCATGGACGAAAAGATCGCGCAATGGGTAATTGCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAcc                                                                                                                                        <  2:1065200/150‑1 (MQ=255)
          tCCGCATGGACGAAAAGCTCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCCCCCTGGGAGATATGAccc                                                                                                                                       <  2:462974/150‑1 (MQ=255)
          tCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAccc                                                                                                                                       <  1:1306564/150‑1 (MQ=255)
          tCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAccc                                                                                                                                       <  1:1789187/150‑1 (MQ=255)
             gCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGt                                                                                                                                    >  1:687470/1‑150 (MQ=255)
              cATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGtt                                                                                                                                   >  1:1479766/1‑150 (MQ=255)
                 ggACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGc                                                                                                                                >  2:460343/1‑150 (MQ=255)
                   aCGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCt                                                                                                                              >  2:7266/1‑150 (MQ=255)
                    cGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGcccg                                                                                                                             <  2:1210573/150‑3 (MQ=255)
                    cGAAAAGATCGCGCAATGCGTAATATCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTGGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                                                                             <  1:952455/150‑1 (MQ=255)
                    cGAAAAGATCGCGCAAAGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCTCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTg                                                                                                                             <  2:114124/150‑1 (MQ=255)
                      aacaGATCCCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGcc                                                                                                                           <  1:1140170/147‑1 (MQ=255)
                      aaaaGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGcc                                                                                                                           <  2:1791172/150‑1 (MQ=255)
                      aaaaGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACACAGTTCGCCTGcc                                                                                                                           >  2:1486489/1‑150 (MQ=255)
                      aaaaGATCGCGCAATGGGTAATTTCGGGGCTGTTGCTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCCGGCACCCAGGAGTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGcc                                                                                                                           <  1:1374175/150‑1 (MQ=255)
                               cgcAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCgtgt                                                                                                                  <  1:1078893/150‑1 (MQ=255)
                                gcAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGtgtg                                                                                                                 <  2:947995/150‑1 (MQ=255)
                                gcAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGGCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGtgtg                                                                                                                 <  2:1783027/150‑1 (MQ=255)
                                 cactGGGGAATTTCGGGGCTGTTGTTCGTACCAGCGGCTTAGCGCCATTCCAGAGCAATTGGTTGCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGc                                                                                                                <  2:920762/147‑1 (MQ=255)
                                 cAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGc                                                                                                                <  1:1300393/150‑1 (MQ=255)
                                   aTGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGAACCAGTTCGCCTGCCCCACCGTGTGCAc                                                                                                              >  1:832703/1‑150 (MQ=255)
                                        tAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTAGGGTCAAATCTCCCACGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTc                                                                                                         >  2:1391668/1‑150 (MQ=255)
                                              cGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCAACACGCGCAGTGTTGTGTCATATCTCCCAGGGGGATATGACCAAGTTCGCCTGCCACACCGTGTTCACACTTCCACGgc                                                                                                   >  2:186430/1‑150 (MQ=255)
                                                  gCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAc                                                                                               >  2:1677249/1‑150 (MQ=255)
                                                     gttgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGt                                                                                            >  1:1552315/1‑150 (MQ=255)
                                                       tgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACCCGCGCAGTGTTGGGTCATATCTCCCAGCGGGATATGAACCAGTTCGCCTGCCACCCAGTCTGCCCCCGTCCACGGCGCACCGTTc                                                                                          >  2:703392/1‑150 (MQ=255)
                                                       tgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTc                                                                                          >  1:1442904/1‑150 (MQ=255)
                                                       tgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTc                                                                                          <  1:699899/150‑1 (MQ=255)
                                                        gttCGTACCAGCCTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCa                                                                                         >  2:499659/1‑150 (MQ=255)
                                                          gcGTCCCAGCGTCGTAGCGCCATTCCAGAGCACTTGGTTCCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGGCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGGTCATc                                                                                       <  2:302679/149‑1 (MQ=255)
                                                            gTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCca                                                                                     >  1:1219405/1‑150 (MQ=255)
                                                               ccAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAAGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCccac                                                                                  >  2:159464/1‑150 (MQ=255)
                                                               ccAGCGTATTAGCGCCATTCCAGAGCAATTGGATTCCAGGCACCCAAGATTGCCACACGCGCAGTCTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCTCATGCCACACCGTGTGCACCCGTACACGGCGCCCCGTTAATacacac                                                                                  >  2:159466/1‑150 (MQ=255)
                                                               ccAGCGGCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCacac                                                                                  <  1:224986/150‑1 (MQ=255)
                                                                ccgCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACt                                                                                 <  1:415131/148‑1 (MQ=255)
                                                                cAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTTCACCCGTACACGGCGCACCGTTCATCCACACt                                                                                 <  2:533518/150‑1 (MQ=255)
                                                                  gCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACAGTTCATCCACACTGc                                                                               >  1:201610/1‑150 (MQ=255)
                                                                  gCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGGGCACCCGTCCACGGCGCACCGTTCATCCACACTGc                                                                               <  1:1034507/150‑1 (MQ=255)
                                                                    gTCTTAGCGCCATTCCAGAGCACTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCCCCCGTCCACGGCACACCGTTCATCCACACTGCGa                                                                             >  1:285561/1‑150 (MQ=255)
                                                                    gTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGa                                                                             >  1:1171243/1‑150 (MQ=255)
                                                                      cTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCACGGGGATATGACCCAGTTCGCCTGCCACCCCGTGTGCACCCGTCCACGGCGCACCGATCATCCACACTGCGacc                                                                           >  1:371493/1‑148 (MQ=255)
                                                                         aGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAcccc                                                                        >  2:828386/1‑150 (MQ=255)
                                                                         aGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAcccc                                                                        >  2:474174/1‑150 (MQ=255)
                                                                             ccATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATTACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATcc                                                                    >  2:1760392/1‑150 (MQ=255)
                                                                              cATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATccc                                                                   >  1:1720659/1‑150 (MQ=255)
                                                                                   cAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGTCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc                                                              >  2:1315594/1‑150 (MQ=255)
                                                                                   cAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCCCCCGTCCACGGCGCACCGTTCCTCCACACTTCGATCCCCCTCCCCGTAc                                                              >  2:1461877/1‑150 (MQ=255)
                                                                                    agagCAATTGGGTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACAGTTTTTGTTAATGATAAGGGGTCAACGTTGATCTACACTGCGAACCCCATCCCCGTAcc                                                             <  2:1603262/150‑1 (MQ=255)
                                                                                       gCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGCCCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCgag                                                          >  1:429693/1‑150 (MQ=255)
                                                                                       gCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCgag                                                          >  1:505093/1‑150 (MQ=255)
                                                                                         aaTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGACCCCCCTCCCCGTACCGAGAt                                                        >  2:895901/1‑150 (MQ=255)
                                                                                          aTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGACCCCCATCCCCGTACCGAGATa                                                       >  1:155197/1‑150 (MQ=255)
                                                                                          aTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGAAATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATa                                                       >  1:149623/1‑150 (MQ=255)
                                                                                                ttCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGgccgc                                                 >  2:1472902/1‑150 (MQ=255)
                                                                                                 tCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGgccgcc                                                <  2:687470/150‑1 (MQ=255)
                                                                                                   cAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAg                                              >  1:1116491/1‑150 (MQ=255)
                                                                                                         cccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAg                                        >  1:1825703/1‑150 (MQ=255)
                                                                                                            aGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACAACGTGTGCACCCGTCCACGGCGCACCGTTCATCCACCCTGCGACCCCCAACCCCGTACCCAGAGAGGCCGCCAGACCCAgttg                                     >  2:819666/1‑150 (MQ=255)
                                                                                                               aTTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCTCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGttgttg                                  <  2:285561/150‑1 (MQ=255)
                                                                                                               aTTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGttgttg                                  <  2:257638/150‑1 (MQ=255)
                                                                                                               aTTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGttgttg                                  <  2:1171243/150‑1 (MQ=255)
                                                                                                                 tGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCg                                <  1:1325727/150‑1 (MQ=255)
                                                                                                                         gcgcAGTGTTGGGTCATAGCTCCCCGGGGGATATGACCCAGTGCGCCTGCCACACCGGGTGCACCCGTCCACGGCGCACCGGTCCGCCCCACTGCGAACCCCATCCCCGTACCAAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCgg                        <  1:873976/150‑1 (MQ=255)
                                                                                                                            cAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGttt                     <  1:1088082/150‑1 (MQ=255)
                                                                                                                              gtgtTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTc                   <  1:186430/150‑1 (MQ=255)
                                                                                                                               tgtTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTct                  <  1:828386/150‑1 (MQ=255)
                                                                                                                               tgtTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTct                  <  1:474174/150‑1 (MQ=255)
                                                                                                                               aactGGGTCATATCCCCCTGGGAGATATTACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAACGTTCATCCACACTGCGAACCCCTTCCCCGGACCGAGATAGGCCGCCAGACCCAGTTGTTGCGTAAGGCGGTTTTct                  >  1:1612460/4‑150 (MQ=255)
                                                                                                                                gtTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTcta                 <  1:606449/150‑1 (MQ=255)
                                                                                                                                gtTGGGGCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGGCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTcta                 <  1:524595/150‑1 (MQ=255)
                                                                                                                                 ttGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTctac                <  2:1825703/150‑1 (MQ=255)
                                                                                                                                  tGGGTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTctact               >  2:454305/1‑150 (MQ=255)
                                                                                                                                  tGGGTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTctact               >  2:403547/1‑150 (MQ=255)
                                                                                                                                   gggTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGACCCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTctacta              >  1:1737328/1‑150 (MQ=255)
                                                                                                                                   gggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGTGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTctacta              >  2:1478022/1‑150 (MQ=255)
                                                                                                                                     gTCATATCCCCCTGGGAGATATGACCCAGTTCGACTGCCCCCCCGTGTGCACCCGTCCTCGGCGCACCGGTAAGACACACTGCGAGCCCCATCCCCGTACCGAGATAGACCGCCAGACCCAGTTGATGCGTAAGGAGGGTGTGTAATACg            >  1:1372934/1‑150 (MQ=255)
                                                                                                                                         tatCTCCCAGGGGGATATGCCCCAGTTCGCCTACCACACCGTGTGCACCCGTCCACGGCGCGCCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGCACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTccc        >  2:966077/1‑150 (MQ=255)
                                                                                                                                           tctcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAg      <  1:612494/150‑1 (MQ=255)
                                                                                                                                           tctcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAATGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAg      <  2:699020/150‑1 (MQ=255)
                                                                                                                                              cccAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGgag   >  2:1028827/1‑150 (MQ=255)
                                                                                                                                               ccAGGTGGATATGACCCAGTTCGCCTGCCACACAGTGTGCTACCGTCCACTGCGCACAGTTCATCCACACTGCGAACACCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGgaga  <  2:188106/150‑1 (MQ=255)
                                                                                                                                               ccAGGGGGATATGACGCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGgaga  <  2:408241/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
AGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGA  >  NC_000913/4307149‑4307441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑