Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,317,977 A→G 5.1% intergenic (‑82/+29) phnL ← / ← phnK ribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase/carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,317,9770AG 5.1% 417.9 / 9.1 136intergenic (‑82/+29)phnL/phnKribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase/carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (67/62);  new base G (2/5);  total (69/67)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 138 total aligned reads displayed.

CGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTC  >  NC_000913/4317829‑4318125
                                                                                                                                                    |                                                                                                                                                    
cGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGATGGGTGTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATaa                                                                                                                                                     <  1:245530/150‑1 (MQ=255)
cGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGATTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTCTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATaa                                                                                                                                                     >  1:582036/1‑150 (MQ=255)
  ggCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAgg                                                                                                                                                   >  2:1276364/1‑150 (MQ=255)
   gCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGc                                                                                                                                                  <  1:407497/150‑1 (MQ=255)
      ggCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGCCCTACGGGGCGCGCATTTGTAGGCCGGATAAGGCGtt                                                                                                                                               >  1:1149041/1‑150 (MQ=255)
      ggCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGtt                                                                                                                                               >  2:431931/1‑150 (MQ=255)
      ggCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGtt                                                                                                                                               <  2:272853/150‑1 (MQ=255)
      ggCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGtt                                                                                                                                               >  2:1372420/1‑150 (MQ=255)
      ggCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTATGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGGATTTGTAGGCCGGATAAGGCGtt                                                                                                                                               <  2:1047954/150‑1 (MQ=255)
        cgcACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTa                                                                                                                                             <  1:423595/150‑1 (MQ=255)
            cGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                         >  2:521930/1‑150 (MQ=255)
            agCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                         <  2:19068/149‑1 (MQ=255)
             gCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCGTTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                        <  2:388830/150‑1 (MQ=255)
              ccGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                       <  1:479825/150‑1 (MQ=255)
                  tttGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                   <  1:1234872/150‑1 (MQ=38)
                   ttGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCACATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                  >  1:624678/1‑150 (MQ=255)
                    tGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTCGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                 >  1:1102720/1‑150 (MQ=255)
                      cTGGTGCATGATTAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTGCGGGTGTCGGATGCTACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                               <  2:1090477/150‑2 (MQ=25)
                      cTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcc                                                                                                                               >  1:590170/1‑149 (MQ=255)
                      cTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                               <  2:1535185/150‑1 (MQ=255)
                      cTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                               >  2:671168/1‑150 (MQ=255)
                       ttgTGCAGGATGAATTTTTTACTGACGATTTGTACGTTAATCATTTTTCGCCTCGTGTTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAc                                                                                                                              <  2:860730/148‑1 (MQ=255)
                       gggTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGAGCGGGTGTCGGATGCGCCGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                                                                                                              <  1:289176/149‑3 (MQ=37)
                        ggTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAcc                                                                                                                             <  2:929332/150‑1 (MQ=255)
                          tGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCgg                                                                                                                           >  2:1635490/1‑150 (MQ=255)
                            cAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCt                                                                                                                         >  1:699259/1‑150 (MQ=255)
                              ggATGAAGGTTTTACTGTCGGTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGGCGGATGCGACGCTGACGCGTCGTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCa                                                                                                                       <  2:959108/150‑1 (MQ=255)
                                ctGAAGGTTTTACTGCCGATTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGTTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAt                                                                                                                     <  1:188274/149‑1 (MQ=255)
                                aTGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGGCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAt                                                                                                                     <  2:558707/150‑1 (MQ=255)
                                 tGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAtt                                                                                                                    >  1:162768/1‑150 (MQ=255)
                                    aGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTg                                                                                                                 <  1:42686/150‑1 (MQ=255)
                                    aGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCACATCCGGCACCGGCTCAATTCTg                                                                                                                 <  2:1325019/150‑1 (MQ=255)
                                     ggTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGTTGTCGGATTCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTATGCCGGATAATTCGTTTACGCCGCATCCGGCACCGGCTCAATTcatc                                                                                                                >  1:1667332/1‑147 (MQ=37)
                                     ggTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGc                                                                                                                <  1:778677/150‑1 (MQ=255)
                                     ggTTTTACTGACGTTTTGTACGTAAATCATTTTTCGCCTCGTGGTTCGGGGGTCGGCTGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGAACCGGCTCAATTCTGc                                                                                                                <  1:1588896/150‑1 (MQ=37)
                                      gTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCa                                                                                                               >  1:1554630/1‑150 (MQ=255)
                                            cTGACGATTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGTAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCg                                                                                                         >  1:881655/1‑150 (MQ=255)
                                             tGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGa                                                                                                        <  2:1154660/150‑1 (MQ=255)
                                               aCGTTTTGTACGTTATTCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATg                                                                                                      <  1:329905/150‑1 (MQ=255)
                                               aCGTTTTGTACGTTAATCATTTTTCGCCTCTTGGTTCGTGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATg                                                                                                      <  2:544380/150‑1 (MQ=255)
                                                      gTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcag                                                                                               >  1:1422393/1‑150 (MQ=255)
                                                       tACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagc                                                                                              >  1:238803/1‑150 (MQ=255)
                                                       tACGTTAATCATTTTTCGCCTCGTCGTTCGGGTGTCGGCTGCGAAGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGCCACaagc                                                                                              >  1:1230836/1‑150 (MQ=25)
                                                        aCGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagca                                                                                             >  1:1134145/1‑150 (MQ=255)
                                                        aCGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagca                                                                                             >  2:410946/1‑150 (MQ=255)
                                                        aCGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagca                                                                                             <  1:899531/150‑1 (MQ=255)
                                                         cGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagcag                                                                                            >  2:132087/1‑150 (MQ=255)
                                                         cGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagcag                                                                                            >  1:1741185/1‑150 (MQ=255)
                                                            tAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtg                                                                                         <  1:1712225/150‑1 (MQ=255)
                                                              aTCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgtg                                                                                       >  1:382944/1‑150 (MQ=255)
                                                              aTCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgtg                                                                                       <  1:1754159/150‑1 (MQ=255)
                                                              aTCATTTTTCGCCTCGTGGTTCGGGTGGAGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgtg                                                                                       <  1:341398/150‑1 (MQ=255)
                                                                cATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCCCCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGta                                                                                     >  1:1360666/1‑150 (MQ=255)
                                                                  tttttCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGtata                                                                                   <  1:821543/150‑1 (MQ=255)
                                                                     ttCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACgg                                                                                <  1:1126940/150‑1 (MQ=255)
                                                                     ttCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCAGTTGTAGGCCTGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACgg                                                                                <  2:1090629/150‑1 (MQ=255)
                                                                       cGCCTCGTGGTTCGGGTGGCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCAATGACACCAGCAGCTGTGTATACGgat                                                                              <  2:1794556/150‑1 (MQ=255)
                                                                         ccTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGgatga                                                                            >  1:1789449/1‑150 (MQ=255)
                                                                               ggTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCggg                                                                      >  1:1045977/1‑150 (MQ=255)
                                                                               ggTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCggg                                                                      <  2:210608/150‑1 (MQ=255)
                                                                                            gATGCGACGCTGACGCGTCTTATCCGACCGACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTGTACGCCGCATCCGGCACCGGCTCAATTCTGCAAACCCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCAcgc                                                         <  1:272006/150‑1 (MQ=255)
                                                                                                cGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtc                                                     >  2:1056982/1‑150 (MQ=255)
                                                                                                cGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGGCGAGCACGcggtc                                                     >  1:1306330/1‑150 (MQ=255)
                                                                                                      ggACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTaa                                               <  1:150542/149‑1 (MQ=255)
                                                                                                       gACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAATCCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAAc                                              >  1:810051/1‑150 (MQ=255)
                                                                                                       gACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAAc                                              >  1:1160236/1‑150 (MQ=255)
                                                                                                        aCGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAAcc                                             <  1:376148/150‑1 (MQ=255)
                                                                                                        aCGCGTCTTATCCGACCGACGGGGAGCGCATTTGGAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTTTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAAcc                                             <  2:1113956/150‑1 (MQ=255)
                                                                                                            gTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAATCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCAct                                         >  1:1036778/1‑150 (MQ=255)
                                                                                                             tCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGGTAACCCActg                                        >  2:349186/1‑149 (MQ=255)
                                                                                                              cTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCActct                                       <  2:789717/150‑1 (MQ=255)
                                                                                                               ttATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACtctc                                      <  1:776887/150‑1 (MQ=255)
                                                                                                                tATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCAACTCTcc                                     >  1:787938/1‑150 (MQ=255)
                                                                                                                 aTCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTcca                                    >  1:29334/1‑150 (MQ=255)
                                                                                                                    cGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTccaccc                                 >  1:129668/1‑149 (MQ=255)
                                                                                                                     gACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCcaccac                                <  2:849575/150‑1 (MQ=255)
                                                                                                                       ccTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACtt                              <  2:1356513/150‑1 (MQ=255)
                                                                                                                       ccTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACtt                              >  1:232508/1‑150 (MQ=255)
                                                                                                                        cTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTg                             <  1:705374/150‑1 (MQ=255)
                                                                                                                         tACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGc                            >  2:179516/1‑150 (MQ=255)
                                                                                                                         tACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGc                            >  1:1623962/1‑150 (MQ=255)
                                                                                                                          aCGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGcc                           >  2:134855/1‑150 (MQ=255)
                                                                                                                             gggAGCGCTTTTGTAGGCCGGATAATGCGTTTACGCCGAATCCGGCACCGGCTCAATTCTACAAAACCTATGACACGAGCAGCTGTGTATTCGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCt                        <  2:695401/150‑1 (MQ=255)
                                                                                                                                aGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCt                     <  2:166668/150‑1 (MQ=255)
                                                                                                                                aGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCt                     <  1:1467126/150‑1 (MQ=255)
                                                                                                                                 gcgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCtt                    <  2:496337/150‑1 (MQ=255)
                                                                                                                                 gcgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCtt                    >  2:970886/1‑150 (MQ=255)
                                                                                                                                  cgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCCCTCTCCACCACTTGCCCCTGCTtc                   >  2:478544/1‑150 (MQ=255)
                                                                                                                                  cgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCTCTGCTtc                   <  2:1467456/150‑1 (MQ=255)
                                                                                                                                  cgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtc                   >  1:746745/1‑150 (MQ=255)
                                                                                                                                  cgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACAGATGACACCAGCAGCTGTGTATACTGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtc                   <  2:124962/150‑1 (MQ=255)
                                                                                                                                    cATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcat                 >  2:212493/1‑150 (MQ=255)
                                                                                                                                    cATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcat                 <  2:1063424/150‑1 (MQ=255)
                                                                                                                                     aTTTGTAGGCCTGATAAGGCGTTTACGCCGCATCCGGCACCGGATCAATTCTGCTAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcatc                <  2:1667332/150‑1 (MQ=255)
                                                                                                                                      tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcatca               <  1:671168/150‑1 (MQ=255)
                                                                                                                                           aGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGc          >  1:1666898/1‑150 (MQ=255)
                                                                                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCAGCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGTGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCaa        >  1:1165341/1‑150 (MQ=255)
                                                                                                                                              ccGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTACCCCACTCTCCCCCACTTGCCCCTGCTTCATCACCAGCaaa       >  1:1189249/1‑150 (MQ=255)
                                                                                                                                              ccGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCaaa       >  1:629345/1‑150 (MQ=255)
                                                                                                                                              ccGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCaaa       >  1:139657/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTC  >  NC_000913/4317829‑4318125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: