Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,317,991 G→T 5.1% intergenic (‑96/+15) phnL ← / ← phnK ribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase/carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,317,9910GT 5.1% 393.5 / 7.1 130intergenic (‑96/+15)phnL/phnKribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase/carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (65/57);  new base T (2/5);  total (67/63)
Fisher's exact test for biased strand distribution p-value = 2.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

Only 100 of 136 total aligned reads displayed.

GCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAGGCGGG  >  NC_000913/4317842‑4318139
                                                                                                                                                     |                                                                                                                                                    
gCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCGTTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                                      <  2:388830/150‑1 (MQ=255)
     tttGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                                 <  1:409421/150‑1 (MQ=38)
     tttGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                                 <  1:1234872/150‑1 (MQ=38)
      ttGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTGCGGGTGGCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                                                <  1:1705388/150‑1 (MQ=37)
         cTGGTGCATGATTAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTGCGGGTGTCGGATGCTACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                             <  2:1090477/150‑2 (MQ=25)
         cTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcc                                                                                                                                             >  1:590170/1‑149 (MQ=255)
         cTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                                             <  2:1535185/150‑1 (MQ=255)
         cTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                                             >  2:671168/1‑150 (MQ=255)
          ttgTGCAGGATGAATTTTTTACTGACGATTTGTACGTTAATCATTTTTCGCCTCGTGTTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAc                                                                                                                                            <  2:860730/148‑1 (MQ=255)
          gggTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGAGCGGGTGTCGGATGCGCCGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                                                                                                                            <  1:289176/149‑3 (MQ=37)
           ggTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAcc                                                                                                                                           <  2:929332/150‑1 (MQ=255)
             tGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTCATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCgg                                                                                                                                         >  1:867219/1‑150 (MQ=255)
                 ggATGAAGGTTTTACTGTCGGTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGGCGGATGCGACGCTGACGCGTCGTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCa                                                                                                                                     <  2:959108/150‑1 (MQ=255)
                   ctGAAGGTTTTACTGCCGATTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGTTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAt                                                                                                                                   <  1:188274/149‑1 (MQ=255)
                   aTGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGGCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAt                                                                                                                                   <  2:558707/150‑1 (MQ=255)
                   aTGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGGTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAt                                                                                                                                   <  1:91317/150‑1 (MQ=255)
                       aGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTg                                                                                                                               <  1:42686/150‑1 (MQ=255)
                        ggTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGc                                                                                                                              <  1:778677/150‑1 (MQ=255)
                        ggTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGc                                                                                                                              <  2:437567/150‑1 (MQ=255)
                        ggTTTTACTGACGTTTTGTACGTAAATCATTTTTCGCCTCGTGGTTCGGGGGTCGGCTGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGAACCGGCTCAATTCTGc                                                                                                                              <  1:1588896/150‑1 (MQ=37)
                               cTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCg                                                                                                                       >  1:118081/1‑150 (MQ=255)
                               cTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGGTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCg                                                                                                                       <  2:1262316/150‑1 (MQ=255)
                                 gACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                                     <  1:60082/150‑1 (MQ=255)
                                       ttGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTTGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACAcc                                                                                                               <  2:832552/150‑1 (MQ=255)
                                          tACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagc                                                                                                            >  1:238803/1‑150 (MQ=255)
                                          tACGTTAATCATTTTTCGCCTCGTCGTTCGGGTGTCGGCTGCGAAGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGCCACaagc                                                                                                            >  1:1230836/1‑150 (MQ=25)
                                           aCGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagca                                                                                                           <  1:899531/150‑1 (MQ=255)
                                           aCGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagca                                                                                                           >  2:410946/1‑150 (MQ=255)
                                           aCGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagca                                                                                                           >  1:1134145/1‑150 (MQ=255)
                                            cGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagcag                                                                                                          >  2:132087/1‑150 (MQ=255)
                                            cGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagcag                                                                                                          >  1:1741185/1‑150 (MQ=255)
                                               tAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCCGCtg                                                                                                       >  2:112106/1‑150 (MQ=255)
                                               tAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtg                                                                                                       <  1:1712225/150‑1 (MQ=255)
                                                 aTCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgtg                                                                                                     >  1:382944/1‑150 (MQ=255)
                                                 aTCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgtg                                                                                                     <  1:1276364/150‑1 (MQ=255)
                                                 aTCATTTTTCGCCTCGTGGTTCGGGTGGAGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgtg                                                                                                     <  1:341398/150‑1 (MQ=255)
                                                   cATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCCCCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGta                                                                                                   >  1:1360666/1‑150 (MQ=255)
                                                   cATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGta                                                                                                   <  1:1586191/150‑1 (MQ=255)
                                                    aTTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGtat                                                                                                  >  1:357621/1‑150 (MQ=255)
                                                     tttttCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGtata                                                                                                 <  1:821543/150‑1 (MQ=255)
                                                     tttttCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGGGtata                                                                                                 >  2:1326579/1‑150 (MQ=255)
                                                     tttttCGCCTCGTGGTTCGGGTGGCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGtata                                                                                                 <  1:57402/150‑1 (MQ=255)
                                                        ttCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACgg                                                                                              <  1:1126940/150‑1 (MQ=255)
                                                        ttCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACgg                                                                                              <  1:1121013/150‑1 (MQ=255)
                                                          cGCCTCGTGGTTCGGGTGGCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCAATGACACCAGCAGCTGTGTATACGgat                                                                                            <  2:1794556/150‑1 (MQ=255)
                                                            ccTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGgatga                                                                                          >  1:1789449/1‑150 (MQ=255)
                                                                  ggTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCggg                                                                                    <  2:210608/150‑1 (MQ=255)
                                                                  ggTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCggg                                                                                    >  1:1045977/1‑150 (MQ=255)
                                                                             cGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCAc                                                                         <  1:783760/150‑1 (MQ=255)
                                                                                   cGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGGCGAGCACGcggtc                                                                   >  1:1306330/1‑150 (MQ=255)
                                                                                         ggACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTaa                                                             <  1:150542/149‑1 (MQ=255)
                                                                                          gACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAATCCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAAc                                                            >  1:810051/1‑150 (MQ=255)
                                                                                          gACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAAc                                                            >  1:1160236/1‑150 (MQ=255)
                                                                                           aCGCGTCTTATCCGACCGACGGGGAGCGCATTTGGAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTTTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAAcc                                                           <  2:1113956/150‑1 (MQ=255)
                                                                                               gTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAATCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCAct                                                       >  1:1036778/1‑150 (MQ=255)
                                                                                                tCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGGTAACCCActg                                                      >  2:349186/1‑149 (MQ=255)
                                                                                                 cTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCActct                                                     <  2:789717/150‑1 (MQ=255)
                                                                                                 cTTATCCGAACTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGAAAAACCGATGACCCCGGCAGCTGTGAATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTACCACActct                                                     >  1:1424856/1‑150 (MQ=37)
                                                                                                  ttATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACtctc                                                    <  1:776887/150‑1 (MQ=255)
                                                                                                   tATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTcc                                                   >  1:1637331/1‑150 (MQ=255)
                                                                                                    aTCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTcca                                                  >  1:29334/1‑150 (MQ=255)
                                                                                                     tCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCCTCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGCTGCGGGTCGTCGAGCACGCGGTCGGTTAACCCCCTCTccac                                                 >  2:1077292/1‑150 (MQ=255)
                                                                                                        gACCTACGGGGACCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAACCCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGGTAAGCCACTCTccaccgc                                              >  2:158218/1‑148 (MQ=255)
                                                                                                          ccTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACtt                                            >  1:232508/1‑150 (MQ=255)
                                                                                                          ccTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACtt                                            <  2:1356513/150‑1 (MQ=255)
                                                                                                           cTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTg                                           <  1:705374/150‑1 (MQ=255)
                                                                                                            tACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGc                                          >  1:1623962/1‑150 (MQ=255)
                                                                                                            tACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGc                                          >  2:179516/1‑150 (MQ=255)
                                                                                                             aCGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGcc                                         >  2:134855/1‑150 (MQ=255)
                                                                                                                gggAGCGCTTTTGTAGGCCGGATAATGCGTTTACGCCGAATCCGGCACCGGCTCAATTCTACAAAACCTATGACACGAGCAGCTGTGTATTCGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCt                                      <  2:695401/150‑1 (MQ=255)
                                                                                                                   aGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCt                                   <  1:1467126/150‑1 (MQ=255)
                                                                                                                   aGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCt                                   <  2:166668/150‑1 (MQ=255)
                                                                                                                    gcgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCtt                                  >  2:970886/1‑150 (MQ=255)
                                                                                                                    gcgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCtt                                  <  2:496337/150‑1 (MQ=255)
                                                                                                                     cgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCTCTGCTtc                                 <  2:1467456/150‑1 (MQ=255)
                                                                                                                     cgcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACAGATGACACCAGCAGCTGTGTATACTGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtc                                 <  2:124962/150‑1 (MQ=255)
                                                                                                                       cATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcat                               <  2:1063424/150‑1 (MQ=255)
                                                                                                                       cATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcat                               >  2:212493/1‑150 (MQ=255)
                                                                                                                        aTTTGTAGGCCTGATAAGGCGTTTACGCCGCATCCGGCACCGGATCAATTCTGCTAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcatc                              <  2:1667332/150‑1 (MQ=255)
                                                                                                                         tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcatca                             <  1:671168/150‑1 (MQ=255)
                                                                                                                             ggtgCCGGATGCGGCGTAAACGCCTTATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAg                         >  1:1773115/4‑150 (MQ=255)
                                                                                                                              aGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGc                        >  1:1666898/1‑150 (MQ=255)
                                                                                                                              aGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGc                        >  1:7181/1‑150 (MQ=255)
                                                                                                                               tgCCGGATGCGGCGTAAACGCCTTATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCTCCAGCa                       >  1:324987/2‑150 (MQ=255)
                                                                                                                                gCCGGATAAGGCGTTTACGCCGCATCCGGCAGCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGTGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCaa                      >  1:1165341/1‑150 (MQ=255)
                                                                                                                                gCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCaa                      >  2:529278/1‑150 (MQ=255)
                                                                                                                                 ccGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCaaa                     >  1:629345/1‑150 (MQ=255)
                                                                                                                                 ccGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCaaa                     >  1:139657/1‑150 (MQ=255)
                                                                                                                                      tAAGGCGTTTACGCCGCCTCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGCGCACGCGGTCGGTTAACCCACTCTCCCCCACTTGCCCCTGCTGCCTCCCCAGCAAACGGTc                >  1:248775/1‑150 (MQ=255)
                                                                                                                                        aGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCCCTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCg              >  2:218702/1‑150 (MQ=255)
                                                                                                                                        aGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCg              >  2:375669/1‑150 (MQ=255)
                                                                                                                                        aGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCg              <  1:1398899/150‑1 (MQ=255)
                                                                                                                                         ggCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGc             >  1:1386173/1‑150 (MQ=255)
                                                                                                                                          gCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGcc            <  2:867219/150‑1 (MQ=255)
                                                                                                                                            gTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCTACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCcag          >  1:1019272/1‑150 (MQ=255)
                                                                                                                                            gTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCcag          <  2:617415/150‑1 (MQ=255)
                                                                                                                                             tttACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCcagc         <  1:954217/150‑1 (MQ=255)
                                                                                                                                                 cgccgcATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAggc     >  2:472933/1‑150 (MQ=255)
                                                                                                                                                  gccgcATCCGGCACCGGATCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAggcg    >  1:6325/1‑150 (MQ=255)
                                                                                                                                                    cgcATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAggcggg  >  2:1793961/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAGGCGGG  >  NC_000913/4317842‑4318139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑